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P50295 (ARY2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Arylamine N-acetyltransferase 2

EC=2.3.1.5
Alternative name(s):
Arylamide acetylase 2
N-acetyltransferase type 2
Short name=NAT-2
Gene names
Name:Nat2
Synonyms:Aac2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length290 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Participates in the detoxification of a plethora of hydrazine and arylamine drugs. 2-aminofluorene and p-aminobenzoic acid (PABA) are preferred substrates for NAT-2. Less activity with anisidine and barely detectable with SMZ.

Catalytic activity

Acetyl-CoA + an arylamine = CoA + an N-acetylarylamine.

Subcellular location

Cytoplasm.

Polymorphism

There are two forms of NAT2: a rapid/stable isoform (Asn-99)and a slow/unstable isoform (Ile-99)

Sequence similarities

Belongs to the arylamine N-acetyltransferase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   Coding sequence diversityPolymorphism
   Molecular functionAcyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionarylamine N-acetyltransferase activity

Inferred from direct assay PubMed 15872051Ref.3. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 290290Arylamine N-acetyltransferase 2
PRO_0000107909

Regions

Region106 – 1072Substrate binding By similarity

Sites

Active site681Acyl-thioester intermediate By similarity
Active site1071 By similarity
Active site1221 By similarity
Binding site1031Coenzyme A By similarity
Binding site1041Coenzyme A; via amide nitrogen By similarity
Binding site2081Coenzyme A By similarity

Natural variations

Natural variant991N → I in allele NAT2*9; slow/unstable isoform.

Sequences

Sequence LengthMass (Da)Tools
P50295 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 704E000DE48CE557

FASTA29033,701
        10         20         30         40         50         60 
MDIEAYFERI GYQSTRSKLD LKTLTEILQH QIRAIPFENL NIHCGESMEL SLEAIFDQIV 

        70         80         90        100        110        120 
RKKRGGWCLQ VNHLLYWALT KLGFETTMLG GYVFNTPANK YSSGMIHLLV QVTISGKDYI 

       130        140        150        160        170        180 
VDAGFGRSYQ MWEPLELTSG KDQPQVPAIF RLTEENGTWY LDQIRREQYV PNQEFINSDL 

       190        200        210        220        230        240 
LEKNKYRKIY SFTLEPRTIE DFESMNTYLQ TSPASVFTSK SFCSLQTPEG VHCLVGSTLT 

       250        260        270        280        290 
YRRFSYKDNV DLVEFKSLTE EEIEDVLRTI FGVSLERKLV PKHGDRFFTI 

« Hide

References

[1]"Molecular genetic basis of rapid and slow acetylation in mice."
Martell K.J., Vatsis K.P., Weber W.W.
Mol. Pharmacol. 40:218-227(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: A/J and C57BL/6.
[2]Hein D.W., Doll M.A.
Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: C3H/HeJ.
Tissue: Heart.
[3]"Arylamine N-acetyltransferase in Balb/c mice: identification of a novel mouse isoenzyme by cloning and expression in vitro."
Kelly S.L., Sim E.
Biochem. J. 302:347-353(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE, CHARACTERIZATION.
Strain: BALB/c.
Tissue: Liver.
[4]"Cloned mouse N-acetyltransferases: enzymatic properties of expressed Nat-1 and Nat-2 gene products."
Martell K.J., Levy G.N., Weber W.W.
Mol. Pharmacol. 42:265-272(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U35886 mRNA. Translation: AAA78943.1.
U35887 mRNA. Translation: AAA78944.1.
U37249 Genomic DNA. Translation: AAA80353.1.
U37250 Genomic DNA. Translation: AAA80354.1.
CCDSCCDS22338.1.
PIRB61267.
RefSeqNP_001162049.1. NM_001168577.1.
NP_035004.1. NM_010874.3.
UniGeneMm.4695.

3D structure databases

ProteinModelPortalP50295.
SMRP50295. Positions 2-290.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-1855751.

Chemistry

BindingDBP50295.
ChEMBLCHEMBL5724.

PTM databases

PhosphoSiteP50295.

Proteomic databases

MaxQBP50295.
PaxDbP50295.
PRIDEP50295.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000093470; ENSMUSP00000091181; ENSMUSG00000051147.
ENSMUST00000163856; ENSMUSP00000130065; ENSMUSG00000051147.
GeneID17961.
KEGGmmu:17961.
UCSCuc009lvw.2. mouse.

Organism-specific databases

CTD10.
MGIMGI:109201. Nat2.

Phylogenomic databases

eggNOGCOG2162.
HOGENOMHOG000205435.
HOVERGENHBG000165.
InParanoidP50295.
KOK00622.
OMAISLICIN.
OrthoDBEOG7BP83H.
PhylomeDBP50295.
TreeFamTF106311.

Enzyme and pathway databases

BRENDA2.3.1.5. 3474.

Gene expression databases

BgeeP50295.
GenevestigatorP50295.

Family and domain databases

InterProIPR001447. Arylamine_N-AcTrfase.
[Graphical view]
PANTHERPTHR11786. PTHR11786. 1 hit.
PfamPF00797. Acetyltransf_2. 1 hit.
[Graphical view]
PRINTSPR01543. ANATRNSFRASE.
ProtoNetSearch...

Other

NextBio292887.
PROP50295.
SOURCESearch...

Entry information

Entry nameARY2_MOUSE
AccessionPrimary (citable) accession number: P50295
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 9, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot