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Protein

Arylamine N-acetyltransferase 2

Gene

Nat2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Participates in the detoxification of a plethora of hydrazine and arylamine drugs. 2-aminofluorene and p-aminobenzoic acid (PABA) are preferred substrates for NAT-2. Less activity with anisidine and barely detectable with SMZ.

Catalytic activityi

Acetyl-CoA + an arylamine = CoA + an N-acetylarylamine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei68 – 681Acyl-thioester intermediateBy similarity
Binding sitei103 – 1031Coenzyme ABy similarity
Binding sitei104 – 1041Coenzyme A; via amide nitrogenBy similarity
Active sitei107 – 1071By similarity
Active sitei122 – 1221By similarity
Binding sitei208 – 2081Coenzyme ABy similarity

GO - Molecular functioni

  1. arylamine N-acetyltransferase activity Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.3.1.5. 3474.
ReactomeiREACT_237536. Acetylation.

Names & Taxonomyi

Protein namesi
Recommended name:
Arylamine N-acetyltransferase 2 (EC:2.3.1.5)
Alternative name(s):
Arylamide acetylase 2
N-acetyltransferase type 2
Short name:
NAT-2
Gene namesi
Name:Nat2
Synonyms:Aac2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 8

Organism-specific databases

MGIiMGI:109201. Nat2.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 290290Arylamine N-acetyltransferase 2PRO_0000107909Add
BLAST

Proteomic databases

MaxQBiP50295.
PaxDbiP50295.
PRIDEiP50295.

PTM databases

PhosphoSiteiP50295.

Expressioni

Gene expression databases

BgeeiP50295.
ExpressionAtlasiP50295. baseline and differential.
GenevestigatoriP50295.

Interactioni

Protein-protein interaction databases

MINTiMINT-1855751.

Structurei

3D structure databases

ProteinModelPortaliP50295.
SMRiP50295. Positions 2-290.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni106 – 1072Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2162.
HOGENOMiHOG000205435.
HOVERGENiHBG000165.
InParanoidiP50295.
KOiK00622.
OMAiTHRRFNY.
OrthoDBiEOG7BP83H.
PhylomeDBiP50295.
TreeFamiTF106311.

Family and domain databases

InterProiIPR001447. Arylamine_N-AcTrfase.
[Graphical view]
PANTHERiPTHR11786. PTHR11786. 1 hit.
PfamiPF00797. Acetyltransf_2. 1 hit.
[Graphical view]
PRINTSiPR01543. ANATRNSFRASE.

Sequencei

Sequence statusi: Complete.

P50295-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDIEAYFERI GYQSTRSKLD LKTLTEILQH QIRAIPFENL NIHCGESMEL
60 70 80 90 100
SLEAIFDQIV RKKRGGWCLQ VNHLLYWALT KLGFETTMLG GYVFNTPANK
110 120 130 140 150
YSSGMIHLLV QVTISGKDYI VDAGFGRSYQ MWEPLELTSG KDQPQVPAIF
160 170 180 190 200
RLTEENGTWY LDQIRREQYV PNQEFINSDL LEKNKYRKIY SFTLEPRTIE
210 220 230 240 250
DFESMNTYLQ TSPASVFTSK SFCSLQTPEG VHCLVGSTLT YRRFSYKDNV
260 270 280 290
DLVEFKSLTE EEIEDVLRTI FGVSLERKLV PKHGDRFFTI
Length:290
Mass (Da):33,701
Last modified:October 1, 1996 - v1
Checksum:i704E000DE48CE557
GO

Polymorphismi

There are two forms of NAT2: a rapid/stable isoform (Asn-99) and a slow/unstable isoform (Ile-99).

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti99 – 991N → I in allele NAT2*9; slow/unstable isoform.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35886 mRNA. Translation: AAA78943.1.
U35887 mRNA. Translation: AAA78944.1.
U37249 Genomic DNA. Translation: AAA80353.1.
U37250 Genomic DNA. Translation: AAA80354.1.
CCDSiCCDS22338.1.
PIRiB61267.
RefSeqiNP_001162049.1. NM_001168577.1.
NP_035004.1. NM_010874.3.
UniGeneiMm.4695.

Genome annotation databases

EnsembliENSMUST00000093470; ENSMUSP00000091181; ENSMUSG00000051147.
ENSMUST00000163856; ENSMUSP00000130065; ENSMUSG00000051147.
GeneIDi17961.
KEGGimmu:17961.
UCSCiuc009lvw.2. mouse.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35886 mRNA. Translation: AAA78943.1.
U35887 mRNA. Translation: AAA78944.1.
U37249 Genomic DNA. Translation: AAA80353.1.
U37250 Genomic DNA. Translation: AAA80354.1.
CCDSiCCDS22338.1.
PIRiB61267.
RefSeqiNP_001162049.1. NM_001168577.1.
NP_035004.1. NM_010874.3.
UniGeneiMm.4695.

3D structure databases

ProteinModelPortaliP50295.
SMRiP50295. Positions 2-290.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1855751.

Chemistry

BindingDBiP50295.
ChEMBLiCHEMBL5724.

PTM databases

PhosphoSiteiP50295.

Proteomic databases

MaxQBiP50295.
PaxDbiP50295.
PRIDEiP50295.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000093470; ENSMUSP00000091181; ENSMUSG00000051147.
ENSMUST00000163856; ENSMUSP00000130065; ENSMUSG00000051147.
GeneIDi17961.
KEGGimmu:17961.
UCSCiuc009lvw.2. mouse.

Organism-specific databases

CTDi10.
MGIiMGI:109201. Nat2.

Phylogenomic databases

eggNOGiCOG2162.
HOGENOMiHOG000205435.
HOVERGENiHBG000165.
InParanoidiP50295.
KOiK00622.
OMAiTHRRFNY.
OrthoDBiEOG7BP83H.
PhylomeDBiP50295.
TreeFamiTF106311.

Enzyme and pathway databases

BRENDAi2.3.1.5. 3474.
ReactomeiREACT_237536. Acetylation.

Miscellaneous databases

NextBioi292887.
PROiP50295.
SOURCEiSearch...

Gene expression databases

BgeeiP50295.
ExpressionAtlasiP50295. baseline and differential.
GenevestigatoriP50295.

Family and domain databases

InterProiIPR001447. Arylamine_N-AcTrfase.
[Graphical view]
PANTHERiPTHR11786. PTHR11786. 1 hit.
PfamiPF00797. Acetyltransf_2. 1 hit.
[Graphical view]
PRINTSiPR01543. ANATRNSFRASE.
ProtoNetiSearch...

Publicationsi

  1. "Molecular genetic basis of rapid and slow acetylation in mice."
    Martell K.J., Vatsis K.P., Weber W.W.
    Mol. Pharmacol. 40:218-227(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: A/J and C57BL/6.
  2. Hein D.W., Doll M.A.
    Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C3H/HeJ.
    Tissue: Heart.
  3. "Arylamine N-acetyltransferase in Balb/c mice: identification of a novel mouse isoenzyme by cloning and expression in vitro."
    Kelly S.L., Sim E.
    Biochem. J. 302:347-353(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE, CHARACTERIZATION.
    Strain: BALB/c.
    Tissue: Liver.
  4. "Cloned mouse N-acetyltransferases: enzymatic properties of expressed Nat-1 and Nat-2 gene products."
    Martell K.J., Levy G.N., Weber W.W.
    Mol. Pharmacol. 42:265-272(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiARY2_MOUSE
AccessioniPrimary (citable) accession number: P50295
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: January 7, 2015
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.