P50295 (ARY2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arylamine N-acetyltransferase 2
EC=2.3.1.5

Alternative name(s):
Arylamide acetylase 2
N-acetyltransferase type 2
Short name=NAT-2

Gene names

Name:	Nat2
Synonyms:	Aac2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	290 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Participates in the detoxification of a plethora of hydrazine and arylamine drugs. 2-aminofluorene and p-aminobenzoic acid (PABA) are preferred substrates for NAT-2. Less activity with anisidine and barely detectable with SMZ.
Catalytic activity	Acetyl-CoA + an arylamine = CoA + an N-acetylarylamine.
Subcellular location	Cytoplasm.
Polymorphism	There are two forms of NAT2: a rapid/stable Asn-99) and a slow/unstable Ile-99).
Sequence similarities	Belongs to the arylamine N-acetyltransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Molecular function	Acyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	arylamine N-acetyltransferase activity Inferred from direct assay Ref.3. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 290	290	Arylamine N-acetyltransferase 2		PRO_0000107909
Regions
	Region	106 – 107	2	Substrate binding By similarity
Sites
	Active site	68	1	Acyl-thioester intermediate By similarity
	Active site	107	1	By similarity
	Active site	122	1	By similarity
	Binding site	103	1	Coenzyme A By similarity
	Binding site	104	1	Coenzyme A; via amide nitrogen By similarity
	Binding site	208	1	Coenzyme A By similarity
Amino acid modifications
	Modified residue	246	1	Phosphotyrosine Ref.5
Natural variations
	Natural variant	99	1	N → I in allele NAT2*9; slow/unstable isoform.

Sequences

	Sequence		Length	Mass (Da)
	P50295 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 704E000DE48CE557	FASTA	290	33,701
	10 20 30 40 50 60 MDIEAYFERI GYQSTRSKLD LKTLTEILQH QIRAIPFENL NIHCGESMEL SLEAIFDQIV 70 80 90 100 110 120 RKKRGGWCLQ VNHLLYWALT KLGFETTMLG GYVFNTPANK YSSGMIHLLV QVTISGKDYI 130 140 150 160 170 180 VDAGFGRSYQ MWEPLELTSG KDQPQVPAIF RLTEENGTWY LDQIRREQYV PNQEFINSDL 190 200 210 220 230 240 LEKNKYRKIY SFTLEPRTIE DFESMNTYLQ TSPASVFTSK SFCSLQTPEG VHCLVGSTLT 250 260 270 280 290 YRRFSYKDNV DLVEFKSLTE EEIEDVLRTI FGVSLERKLV PKHGDRFFTI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular genetic basis of rapid and slow acetylation in mice." Martell K.J., Vatsis K.P., Weber W.W. Mol. Pharmacol. 40:218-227(1991) [PubMed: 1875909] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: A/J and C57BL/6.
[2]	Hein D.W., Doll M.A. Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: C3H/HeJ. Tissue: Heart.
[3]	"Arylamine N-acetyltransferase in Balb/c mice: identification of a novel mouse isoenzyme by cloning and expression in vitro." Kelly S.L., Sim E. Biochem. J. 302:347-353(1994) [PubMed: 7545952] [Abstract] Cited for: NUCLEOTIDE SEQUENCE, CHARACTERIZATION. Strain: BALB/c. Tissue: Liver.
[4]	"Cloned mouse N-acetyltransferases: enzymatic properties of expressed Nat-1 and Nat-2 gene products." Martell K.J., Levy G.N., Weber W.W. Mol. Pharmacol. 42:265-272(1992) [PubMed: 1513324] [Abstract] Cited for: CHARACTERIZATION.
[5]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-246, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U35886 mRNA. Translation: AAA78943.1. U35887 mRNA. Translation: AAA78944.1. U37249 Genomic DNA. Translation: AAA80353.1. U37250 Genomic DNA. Translation: AAA80354.1.
IPI	IPI00116459.
PIR	B61267.
RefSeq	NP_001162049.1. NM_001168577.1. NP_035004.1. NM_010874.3.
UniGene	Mm.4695.
3D structure databases
ProteinModelPortal	P50295.
SMR	P50295. Positions 2-290.
ModBase	Search...
Protein-protein interaction databases
STRING	P50295.
PTM databases
PhosphoSite	P50295.
Proteomic databases
PRIDE	P50295.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000093470; ENSMUSP00000091181; ENSMUSG00000051147. ENSMUST00000163856; ENSMUSP00000130065; ENSMUSG00000051147.
GeneID	17961.
KEGG	mmu:17961.
Organism-specific databases
CTD	10.
MGI	MGI:109201. Nat2.
Phylogenomic databases
eggNOG	roNOG04860.
HOGENOM	HBG544563.
HOVERGEN	HBG000165.
InParanoid	P50295.
OMA	DFESVNI.
OrthoDB	EOG46Q6T2.
PhylomeDB	P50295.
Enzyme and pathway databases
BRENDA	2.3.1.5. 3474.
Gene expression databases
ArrayExpress	P50295.
Bgee	P50295.
Genevestigator	P50295.
GermOnline	ENSMUSG00000051147. Mus musculus.
Family and domain databases
InterPro	IPR001447. N-AcTrfase. [Graphical view]
KO	K00622.
PANTHER	PTHR11786. Acetyltransf2. 1 hit.
Pfam	PF00797. Acetyltransf_2. 1 hit. [Graphical view]
PRINTS	PR01543. ANATRNSFRASE.
ProtoNet	Search...
Other
NextBio	292887.
SOURCE	Search...

Entry information

Entry name

ARY2_MOUSE

Accession

Primary (citable) accession number: P50295

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 16, 2011
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program