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P50295

- ARY2_MOUSE

UniProt

P50295 - ARY2_MOUSE

Protein

Arylamine N-acetyltransferase 2

Gene

Nat2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Participates in the detoxification of a plethora of hydrazine and arylamine drugs. 2-aminofluorene and p-aminobenzoic acid (PABA) are preferred substrates for NAT-2. Less activity with anisidine and barely detectable with SMZ.

    Catalytic activityi

    Acetyl-CoA + an arylamine = CoA + an N-acetylarylamine.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei68 – 681Acyl-thioester intermediateBy similarity
    Binding sitei103 – 1031Coenzyme ABy similarity
    Binding sitei104 – 1041Coenzyme A; via amide nitrogenBy similarity
    Active sitei107 – 1071By similarity
    Active sitei122 – 1221By similarity
    Binding sitei208 – 2081Coenzyme ABy similarity

    GO - Molecular functioni

    1. arylamine N-acetyltransferase activity Source: MGI

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Enzyme and pathway databases

    BRENDAi2.3.1.5. 3474.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Arylamine N-acetyltransferase 2 (EC:2.3.1.5)
    Alternative name(s):
    Arylamide acetylase 2
    N-acetyltransferase type 2
    Short name:
    NAT-2
    Gene namesi
    Name:Nat2
    Synonyms:Aac2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 8

    Organism-specific databases

    MGIiMGI:109201. Nat2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 290290Arylamine N-acetyltransferase 2PRO_0000107909Add
    BLAST

    Proteomic databases

    MaxQBiP50295.
    PaxDbiP50295.
    PRIDEiP50295.

    PTM databases

    PhosphoSiteiP50295.

    Expressioni

    Gene expression databases

    BgeeiP50295.
    GenevestigatoriP50295.

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-1855751.

    Structurei

    3D structure databases

    ProteinModelPortaliP50295.
    SMRiP50295. Positions 2-290.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni106 – 1072Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG2162.
    HOGENOMiHOG000205435.
    HOVERGENiHBG000165.
    InParanoidiP50295.
    KOiK00622.
    OMAiISLICIN.
    OrthoDBiEOG7BP83H.
    PhylomeDBiP50295.
    TreeFamiTF106311.

    Family and domain databases

    InterProiIPR001447. Arylamine_N-AcTrfase.
    [Graphical view]
    PANTHERiPTHR11786. PTHR11786. 1 hit.
    PfamiPF00797. Acetyltransf_2. 1 hit.
    [Graphical view]
    PRINTSiPR01543. ANATRNSFRASE.

    Sequencei

    Sequence statusi: Complete.

    P50295-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDIEAYFERI GYQSTRSKLD LKTLTEILQH QIRAIPFENL NIHCGESMEL    50
    SLEAIFDQIV RKKRGGWCLQ VNHLLYWALT KLGFETTMLG GYVFNTPANK 100
    YSSGMIHLLV QVTISGKDYI VDAGFGRSYQ MWEPLELTSG KDQPQVPAIF 150
    RLTEENGTWY LDQIRREQYV PNQEFINSDL LEKNKYRKIY SFTLEPRTIE 200
    DFESMNTYLQ TSPASVFTSK SFCSLQTPEG VHCLVGSTLT YRRFSYKDNV 250
    DLVEFKSLTE EEIEDVLRTI FGVSLERKLV PKHGDRFFTI 290
    Length:290
    Mass (Da):33,701
    Last modified:October 1, 1996 - v1
    Checksum:i704E000DE48CE557
    GO

    Polymorphismi

    There are two forms of NAT2: a rapid/stable isoform (Asn-99) and a slow/unstable isoform (Ile-99).

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti99 – 991N → I in allele NAT2*9; slow/unstable isoform.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U35886 mRNA. Translation: AAA78943.1.
    U35887 mRNA. Translation: AAA78944.1.
    U37249 Genomic DNA. Translation: AAA80353.1.
    U37250 Genomic DNA. Translation: AAA80354.1.
    CCDSiCCDS22338.1.
    PIRiB61267.
    RefSeqiNP_001162049.1. NM_001168577.1.
    NP_035004.1. NM_010874.3.
    UniGeneiMm.4695.

    Genome annotation databases

    EnsembliENSMUST00000093470; ENSMUSP00000091181; ENSMUSG00000051147.
    ENSMUST00000163856; ENSMUSP00000130065; ENSMUSG00000051147.
    GeneIDi17961.
    KEGGimmu:17961.
    UCSCiuc009lvw.2. mouse.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U35886 mRNA. Translation: AAA78943.1 .
    U35887 mRNA. Translation: AAA78944.1 .
    U37249 Genomic DNA. Translation: AAA80353.1 .
    U37250 Genomic DNA. Translation: AAA80354.1 .
    CCDSi CCDS22338.1.
    PIRi B61267.
    RefSeqi NP_001162049.1. NM_001168577.1.
    NP_035004.1. NM_010874.3.
    UniGenei Mm.4695.

    3D structure databases

    ProteinModelPortali P50295.
    SMRi P50295. Positions 2-290.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-1855751.

    Chemistry

    BindingDBi P50295.
    ChEMBLi CHEMBL5724.

    PTM databases

    PhosphoSitei P50295.

    Proteomic databases

    MaxQBi P50295.
    PaxDbi P50295.
    PRIDEi P50295.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000093470 ; ENSMUSP00000091181 ; ENSMUSG00000051147 .
    ENSMUST00000163856 ; ENSMUSP00000130065 ; ENSMUSG00000051147 .
    GeneIDi 17961.
    KEGGi mmu:17961.
    UCSCi uc009lvw.2. mouse.

    Organism-specific databases

    CTDi 10.
    MGIi MGI:109201. Nat2.

    Phylogenomic databases

    eggNOGi COG2162.
    HOGENOMi HOG000205435.
    HOVERGENi HBG000165.
    InParanoidi P50295.
    KOi K00622.
    OMAi ISLICIN.
    OrthoDBi EOG7BP83H.
    PhylomeDBi P50295.
    TreeFami TF106311.

    Enzyme and pathway databases

    BRENDAi 2.3.1.5. 3474.

    Miscellaneous databases

    NextBioi 292887.
    PROi P50295.
    SOURCEi Search...

    Gene expression databases

    Bgeei P50295.
    Genevestigatori P50295.

    Family and domain databases

    InterProi IPR001447. Arylamine_N-AcTrfase.
    [Graphical view ]
    PANTHERi PTHR11786. PTHR11786. 1 hit.
    Pfami PF00797. Acetyltransf_2. 1 hit.
    [Graphical view ]
    PRINTSi PR01543. ANATRNSFRASE.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular genetic basis of rapid and slow acetylation in mice."
      Martell K.J., Vatsis K.P., Weber W.W.
      Mol. Pharmacol. 40:218-227(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: A/J and C57BL/6.
    2. Hein D.W., Doll M.A.
      Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: C3H/HeJ.
      Tissue: Heart.
    3. "Arylamine N-acetyltransferase in Balb/c mice: identification of a novel mouse isoenzyme by cloning and expression in vitro."
      Kelly S.L., Sim E.
      Biochem. J. 302:347-353(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE, CHARACTERIZATION.
      Strain: BALB/c.
      Tissue: Liver.
    4. "Cloned mouse N-acetyltransferases: enzymatic properties of expressed Nat-1 and Nat-2 gene products."
      Martell K.J., Levy G.N., Weber W.W.
      Mol. Pharmacol. 42:265-272(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.

    Entry informationi

    Entry nameiARY2_MOUSE
    AccessioniPrimary (citable) accession number: P50295
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3