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Reviewed, UniProtKB/Swiss-Prot P50294 (ARY1_MOUSE)

Last modified January 19, 2010. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Arylamine N-acetyltransferase 1
    EC=2.3.1.5
Alternative name(s):
    Arylamide acetylase 1
    N-acetyltransferase type 1
      Short name=NAT-1
Gene names
Name: Nat1
Synonyms: Aac1
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMus

Protein attributes

Sequence length290 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Participates in the detoxification of a plethora of hydrazine and arylamine drugs. Isoniazid, 2-aminofluorene and anisidine are preferred substrates for NAT-1. No activity with p-aminobenzoic acid (PABA) nor SMZ.

Catalytic activity

Acetyl-CoA + an arylamine = CoA + an N-acetylarylamine.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the arylamine N-acetyltransferase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   Molecular functionAcyltransferase
Transferase
Gene Ontology (GO)
   Biological processmetabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionarylamine N-acetyltransferase activity Ref.3

Inferred from direct assay. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 290290Arylamine N-acetyltransferase 1
PRO_0000107908

Regions

Region106 – 1072Substrate binding By similarity

Sites

Active site681Acyl-thioester intermediate By similarity
Active site1071 By similarity
Active site1221 By similarity
Binding site1031Coenzyme A By similarity
Binding site2081Coenzyme A By similarity

Sequences

Sequence LengthMass (Da)Tools
P50294-1 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 228C64130A2AD881

FASTA29033,713
        10         20         30         40         50         60 
MDIEAYFERI GYKNSVNKLD LATLTEVLQH QMRAVPFENL NMHCGEAMHL DLQDIFDHIV 

        70         80         90        100        110        120 
RKKRGGWCLQ VNHLLYWALT KMGFETTMLG GYVYITPVSK YSSEMVHLLV QVTISDRKYI 

       130        140        150        160        170        180 
VDSAYGGSYQ MWEPLELTSG KDQPQVPAIF LLTEENGTWY LDQIRREQYV PNEEFVNSDL 

       190        200        210        220        230        240 
LEKNKYRKIY SFTLEPRVIE DFEYVNSYLQ TSPASVFVST SFCSLQTSEG VHCLVGSTFT 

       250        260        270        280        290 
SRRFSYKDDV DLVEFKYVNE EEIEDVLKTA FGISLERKFV PKHGELVFTI 

« Hide

References

[1]"Molecular genetic basis of rapid and slow acetylation in mice."
Martell K.J., Vatsis K.P., Weber W.W.
Mol. Pharmacol. 40:218-227(1991) [PubMed: 1875909] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: C57BL/6.
[2]Hein D.W., Doll M.A.
Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: C3H/HeJ.
Tissue: Heart.
[3]"Arylamine N-acetyltransferase in Balb/c mice: identification of a novel mouse isoenzyme by cloning and expression in vitro."
Kelly S.L., Sim E.
Biochem. J. 302:347-353(1994) [PubMed: 7545952] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE, CHARACTERIZATION.
Strain: BALB/c.
Tissue: Liver.
[4]"Cloned mouse N-acetyltransferases: enzymatic properties of expressed Nat-1 and Nat-2 gene products."
Martell K.J., Levy G.N., Weber W.W.
Mol. Pharmacol. 42:265-272(1992) [PubMed: 1513324] [Abstract]
Cited for: CHARACTERIZATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U35885 mRNA. Translation: AAA78942.1.
U37119 Genomic DNA. Translation: AAA80667.1.
IPIIPI00116457.
PIRA61267.
RefSeqNP_032699.1.
UniGeneMm.14125

3D structure databases

SMRP50294. Positions 2-290.
ModBaseSearch...

Protein-protein interaction databases

STRINGP50294.

PTM databases

PhosphoSiteP50294.

Proteomic databases

PRIDEP50294.

Genome annotation databases

EnsemblENSMUST00000026677; ENSMUSP00000026677; ENSMUSG00000025588; Mus musculus. [Genome view]
GeneID17960.
KEGGmmu:17960.
UCSCuc009lvv.1. mouse.

Organism-specific databases

CTD17960.
MGIMGI:97279. Nat1.

Phylogenomic databases

eggNOGroNOG04860.
HOGENOMHBG544563.
HOVERGENP50294.
InParanoidP50294.
OMAPVSKYSS.
OrthoDBEOG9QZB6C.
PhylomeDBP50294.

Enzyme and pathway databases

BRENDA2.3.1.5. 244.

Gene expression databases

ArrayExpressP50294.
BgeeP50294.
GenevestigatorP50294.
GermOnlineENSMUSG00000025588. Mus musculus.

Family and domain databases

InterProIPR001447. N-AcTrfase.
[Graphical view]
PANTHERPTHR11786. Acetyltransf2. 1 hit.
PfamPF00797. Acetyltransf_2. 1 hit.
[Graphical view]
PRINTSPR01543. ANATRNSFRASE.
ProtoNetSearch...

Other Resources

NextBio292883.
SOURCESearch...

Entry information

Entry nameARY1_MOUSE
AccessionPrimary (citable) accession number: P50294
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: January 19, 2010
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents