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P50294

- ARY1_MOUSE

UniProt

P50294 - ARY1_MOUSE

Protein

Arylamine N-acetyltransferase 1

Gene

Nat1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Participates in the detoxification of a plethora of hydrazine and arylamine drugs. Isoniazid, 2-aminofluorene and anisidine are preferred substrates for NAT-1. No activity with p-aminobenzoic acid (PABA) nor SMZ.

    Catalytic activityi

    Acetyl-CoA + an arylamine = CoA + an N-acetylarylamine.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei68 – 681Acyl-thioester intermediateBy similarity
    Binding sitei103 – 1031Coenzyme ABy similarity
    Active sitei107 – 1071By similarity
    Active sitei122 – 1221By similarity
    Binding sitei208 – 2081Coenzyme ABy similarity

    GO - Molecular functioni

    1. arylamine N-acetyltransferase activity Source: MGI

    GO - Biological processi

    1. aging Source: Ensembl
    2. heart development Source: Ensembl
    3. liver development Source: Ensembl
    4. response to thyroxine Source: Ensembl

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Enzyme and pathway databases

    BRENDAi2.3.1.5. 3474.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Arylamine N-acetyltransferase 1 (EC:2.3.1.5)
    Alternative name(s):
    Arylamide acetylase 1
    N-acetyltransferase type 1
    Short name:
    NAT-1
    Gene namesi
    Name:Nat1
    Synonyms:Aac1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 8

    Organism-specific databases

    MGIiMGI:97279. Nat1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 290290Arylamine N-acetyltransferase 1PRO_0000107908Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiP50294.
    PRIDEiP50294.

    PTM databases

    PhosphoSiteiP50294.

    Expressioni

    Gene expression databases

    BgeeiP50294.
    GenevestigatoriP50294.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000026677.

    Structurei

    3D structure databases

    ProteinModelPortaliP50294.
    SMRiP50294. Positions 2-290.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni106 – 1072Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG2162.
    HOGENOMiHOG000205435.
    HOVERGENiHBG000165.
    InParanoidiP50294.
    KOiK00622.
    OMAiSKYSSEM.
    OrthoDBiEOG7BP83H.
    PhylomeDBiP50294.
    TreeFamiTF106311.

    Family and domain databases

    InterProiIPR001447. Arylamine_N-AcTrfase.
    [Graphical view]
    PANTHERiPTHR11786. PTHR11786. 1 hit.
    PfamiPF00797. Acetyltransf_2. 1 hit.
    [Graphical view]
    PRINTSiPR01543. ANATRNSFRASE.

    Sequencei

    Sequence statusi: Complete.

    P50294-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDIEAYFERI GYKNSVNKLD LATLTEVLQH QMRAVPFENL NMHCGEAMHL    50
    DLQDIFDHIV RKKRGGWCLQ VNHLLYWALT KMGFETTMLG GYVYITPVSK 100
    YSSEMVHLLV QVTISDRKYI VDSAYGGSYQ MWEPLELTSG KDQPQVPAIF 150
    LLTEENGTWY LDQIRREQYV PNEEFVNSDL LEKNKYRKIY SFTLEPRVIE 200
    DFEYVNSYLQ TSPASVFVST SFCSLQTSEG VHCLVGSTFT SRRFSYKDDV 250
    DLVEFKYVNE EEIEDVLKTA FGISLERKFV PKHGELVFTI 290
    Length:290
    Mass (Da):33,713
    Last modified:October 1, 1996 - v1
    Checksum:i228C64130A2AD881
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U35885 mRNA. Translation: AAA78942.1.
    U37119 Genomic DNA. Translation: AAA80667.1.
    CCDSiCCDS22337.1.
    PIRiA61267.
    RefSeqiNP_032699.1. NM_008673.1.
    XP_006509654.1. XM_006509591.1.
    UniGeneiMm.14125.

    Genome annotation databases

    EnsembliENSMUST00000026677; ENSMUSP00000026677; ENSMUSG00000025588.
    GeneIDi17960.
    KEGGimmu:17960.
    UCSCiuc009lvv.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U35885 mRNA. Translation: AAA78942.1 .
    U37119 Genomic DNA. Translation: AAA80667.1 .
    CCDSi CCDS22337.1.
    PIRi A61267.
    RefSeqi NP_032699.1. NM_008673.1.
    XP_006509654.1. XM_006509591.1.
    UniGenei Mm.14125.

    3D structure databases

    ProteinModelPortali P50294.
    SMRi P50294. Positions 2-290.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000026677.

    Chemistry

    ChEMBLi CHEMBL5723.

    PTM databases

    PhosphoSitei P50294.

    Proteomic databases

    PaxDbi P50294.
    PRIDEi P50294.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000026677 ; ENSMUSP00000026677 ; ENSMUSG00000025588 .
    GeneIDi 17960.
    KEGGi mmu:17960.
    UCSCi uc009lvv.1. mouse.

    Organism-specific databases

    CTDi 9.
    MGIi MGI:97279. Nat1.

    Phylogenomic databases

    eggNOGi COG2162.
    HOGENOMi HOG000205435.
    HOVERGENi HBG000165.
    InParanoidi P50294.
    KOi K00622.
    OMAi SKYSSEM.
    OrthoDBi EOG7BP83H.
    PhylomeDBi P50294.
    TreeFami TF106311.

    Enzyme and pathway databases

    BRENDAi 2.3.1.5. 3474.

    Miscellaneous databases

    NextBioi 292883.
    PROi P50294.
    SOURCEi Search...

    Gene expression databases

    Bgeei P50294.
    Genevestigatori P50294.

    Family and domain databases

    InterProi IPR001447. Arylamine_N-AcTrfase.
    [Graphical view ]
    PANTHERi PTHR11786. PTHR11786. 1 hit.
    Pfami PF00797. Acetyltransf_2. 1 hit.
    [Graphical view ]
    PRINTSi PR01543. ANATRNSFRASE.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular genetic basis of rapid and slow acetylation in mice."
      Martell K.J., Vatsis K.P., Weber W.W.
      Mol. Pharmacol. 40:218-227(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: C57BL/6.
    2. Hein D.W., Doll M.A.
      Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: C3H/HeJ.
      Tissue: Heart.
    3. "Arylamine N-acetyltransferase in Balb/c mice: identification of a novel mouse isoenzyme by cloning and expression in vitro."
      Kelly S.L., Sim E.
      Biochem. J. 302:347-353(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE, CHARACTERIZATION.
      Strain: BALB/c.
      Tissue: Liver.
    4. "Cloned mouse N-acetyltransferases: enzymatic properties of expressed Nat-1 and Nat-2 gene products."
      Martell K.J., Levy G.N., Weber W.W.
      Mol. Pharmacol. 42:265-272(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.

    Entry informationi

    Entry nameiARY1_MOUSE
    AccessioniPrimary (citable) accession number: P50294
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3