P50294 (ARY1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arylamine N-acetyltransferase 1
EC=2.3.1.5

Alternative name(s):
Arylamide acetylase 1
N-acetyltransferase type 1
Short name=NAT-1

Gene names

Name:	Nat1
Synonyms:	Aac1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	290 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Participates in the detoxification of a plethora of hydrazine and arylamine drugs. Isoniazid, 2-aminofluorene and anisidine are preferred substrates for NAT-1. No activity with p-aminobenzoic acid (PABA) nor SMZ.
Catalytic activity	Acetyl-CoA + an arylamine = CoA + an N-acetylarylamine.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the arylamine N-acetyltransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	aging Inferred from electronic annotation. Source: Compara heart development Inferred from electronic annotation. Source: Compara liver development Inferred from electronic annotation. Source: Compara response to thyroxine stimulus Inferred from electronic annotation. Source: Compara
Cellular_component	cytosol Inferred from electronic annotation. Source: Compara
Molecular_function	arylamine N-acetyltransferase activity Inferred from direct assay Ref.3. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 290	290	Arylamine N-acetyltransferase 1		PRO_0000107908
Regions
	Region	106 – 107	2	Substrate binding By similarity
Sites
	Active site	68	1	Acyl-thioester intermediate By similarity
	Active site	107	1	By similarity
	Active site	122	1	By similarity
	Binding site	103	1	Coenzyme A By similarity
	Binding site	208	1	Coenzyme A By similarity

Sequences

	Sequence		Length	Mass (Da)
	P50294 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 228C64130A2AD881	FASTA	290	33,713
	10 20 30 40 50 60 MDIEAYFERI GYKNSVNKLD LATLTEVLQH QMRAVPFENL NMHCGEAMHL DLQDIFDHIV 70 80 90 100 110 120 RKKRGGWCLQ VNHLLYWALT KMGFETTMLG GYVYITPVSK YSSEMVHLLV QVTISDRKYI 130 140 150 160 170 180 VDSAYGGSYQ MWEPLELTSG KDQPQVPAIF LLTEENGTWY LDQIRREQYV PNEEFVNSDL 190 200 210 220 230 240 LEKNKYRKIY SFTLEPRVIE DFEYVNSYLQ TSPASVFVST SFCSLQTSEG VHCLVGSTFT 250 260 270 280 290 SRRFSYKDDV DLVEFKYVNE EEIEDVLKTA FGISLERKFV PKHGELVFTI

References

[1]	"Molecular genetic basis of rapid and slow acetylation in mice." Martell K.J., Vatsis K.P., Weber W.W. Mol. Pharmacol. 40:218-227(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: C57BL/6.
[2]	Hein D.W., Doll M.A. Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: C3H/HeJ. Tissue: Heart.
[3]	"Arylamine N-acetyltransferase in Balb/c mice: identification of a novel mouse isoenzyme by cloning and expression in vitro." Kelly S.L., Sim E. Biochem. J. 302:347-353(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE, CHARACTERIZATION. Strain: BALB/c. Tissue: Liver.
[4]	"Cloned mouse N-acetyltransferases: enzymatic properties of expressed Nat-1 and Nat-2 gene products." Martell K.J., Levy G.N., Weber W.W. Mol. Pharmacol. 42:265-272(1992) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U35885 mRNA. Translation: AAA78942.1. U37119 Genomic DNA. Translation: AAA80667.1.
IPI	IPI00116457.
PIR	A61267.
RefSeq	NP_032699.1. NM_008673.1.
UniGene	Mm.14125.
3D structure databases
ProteinModelPortal	P50294.
SMR	P50294. Positions 2-290.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000026677.
PTM databases
PhosphoSite	P50294.
Proteomic databases
PaxDb	P50294.
PRIDE	P50294.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000026677; ENSMUSP00000026677; ENSMUSG00000025588.
GeneID	17960.
KEGG	mmu:17960.
Organism-specific databases
CTD	9.
MGI	MGI:97279. Nat1.
Phylogenomic databases
eggNOG	COG2162.
HOGENOM	HOG000205435.
HOVERGEN	HBG000165.
InParanoid	P50294.
KO	K00622.
OMA	CEETMEL.
OrthoDB	EOG46Q6T2.
Enzyme and pathway databases
BRENDA	2.3.1.5. 3474.
Gene expression databases
ArrayExpress	P50294.
Bgee	P50294.
Genevestigator	P50294.
GermOnline	ENSMUSG00000025588. Mus musculus.
Family and domain databases
InterPro	IPR001447. Arylamine_N-AcTrfase. [Graphical view]
PANTHER	PTHR11786. PTHR11786. 1 hit.
Pfam	PF00797. Acetyltransf_2. 1 hit. [Graphical view]
PRINTS	PR01543. ANATRNSFRASE.
ProtoNet	Search...
Other
ChEMBL	CHEMBL5723.
NextBio	292883.
SOURCE	Search...

Entry information

Entry name

ARY1_MOUSE

Accession

Primary (citable) accession number: P50294

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program