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Protein

Arylamine N-acetyltransferase 1

Gene

Nat1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Participates in the detoxification of a plethora of hydrazine and arylamine drugs. Isoniazid, 2-aminofluorene and anisidine are preferred substrates for NAT-1. No activity with p-aminobenzoic acid (PABA) nor SMZ.

Catalytic activityi

Acetyl-CoA + an arylamine = CoA + an N-acetylarylamine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei68 – 681Acyl-thioester intermediateBy similarity
Binding sitei103 – 1031Coenzyme ABy similarity
Active sitei107 – 1071By similarity
Active sitei122 – 1221By similarity
Binding sitei208 – 2081Coenzyme ABy similarity

GO - Molecular functioni

  • arylamine N-acetyltransferase activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.3.1.5. 3474.
ReactomeiREACT_343875. Acetylation.

Names & Taxonomyi

Protein namesi
Recommended name:
Arylamine N-acetyltransferase 1 (EC:2.3.1.5)
Alternative name(s):
Arylamide acetylase 1
N-acetyltransferase type 1
Short name:
NAT-1
Gene namesi
Name:Nat1
Synonyms:Aac1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:97279. Nat1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 290290Arylamine N-acetyltransferase 1PRO_0000107908Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP50294.
PRIDEiP50294.

PTM databases

PhosphoSiteiP50294.

Expressioni

Gene expression databases

BgeeiP50294.
ExpressionAtlasiP50294. baseline and differential.
GenevisibleiP50294. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000026677.

Structurei

3D structure databases

ProteinModelPortaliP50294.
SMRiP50294. Positions 2-290.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni106 – 1072Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2162.
HOGENOMiHOG000205435.
HOVERGENiHBG000165.
InParanoidiP50294.
KOiK00622.
OMAiPATHTLM.
OrthoDBiEOG7BP83H.
PhylomeDBiP50294.
TreeFamiTF106311.

Family and domain databases

InterProiIPR001447. Arylamine_N-AcTrfase.
[Graphical view]
PANTHERiPTHR11786. PTHR11786. 1 hit.
PfamiPF00797. Acetyltransf_2. 1 hit.
[Graphical view]
PRINTSiPR01543. ANATRNSFRASE.

Sequencei

Sequence statusi: Complete.

P50294-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDIEAYFERI GYKNSVNKLD LATLTEVLQH QMRAVPFENL NMHCGEAMHL
60 70 80 90 100
DLQDIFDHIV RKKRGGWCLQ VNHLLYWALT KMGFETTMLG GYVYITPVSK
110 120 130 140 150
YSSEMVHLLV QVTISDRKYI VDSAYGGSYQ MWEPLELTSG KDQPQVPAIF
160 170 180 190 200
LLTEENGTWY LDQIRREQYV PNEEFVNSDL LEKNKYRKIY SFTLEPRVIE
210 220 230 240 250
DFEYVNSYLQ TSPASVFVST SFCSLQTSEG VHCLVGSTFT SRRFSYKDDV
260 270 280 290
DLVEFKYVNE EEIEDVLKTA FGISLERKFV PKHGELVFTI
Length:290
Mass (Da):33,713
Last modified:October 1, 1996 - v1
Checksum:i228C64130A2AD881
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35885 mRNA. Translation: AAA78942.1.
U37119 Genomic DNA. Translation: AAA80667.1.
CCDSiCCDS22337.1.
PIRiA61267.
RefSeqiNP_032699.1. NM_008673.1.
UniGeneiMm.14125.

Genome annotation databases

EnsembliENSMUST00000026677; ENSMUSP00000026677; ENSMUSG00000025588.
GeneIDi17960.
KEGGimmu:17960.
UCSCiuc009lvv.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35885 mRNA. Translation: AAA78942.1.
U37119 Genomic DNA. Translation: AAA80667.1.
CCDSiCCDS22337.1.
PIRiA61267.
RefSeqiNP_032699.1. NM_008673.1.
UniGeneiMm.14125.

3D structure databases

ProteinModelPortaliP50294.
SMRiP50294. Positions 2-290.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000026677.

Chemistry

ChEMBLiCHEMBL5723.

PTM databases

PhosphoSiteiP50294.

Proteomic databases

PaxDbiP50294.
PRIDEiP50294.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026677; ENSMUSP00000026677; ENSMUSG00000025588.
GeneIDi17960.
KEGGimmu:17960.
UCSCiuc009lvv.1. mouse.

Organism-specific databases

CTDi9.
MGIiMGI:97279. Nat1.

Phylogenomic databases

eggNOGiCOG2162.
HOGENOMiHOG000205435.
HOVERGENiHBG000165.
InParanoidiP50294.
KOiK00622.
OMAiPATHTLM.
OrthoDBiEOG7BP83H.
PhylomeDBiP50294.
TreeFamiTF106311.

Enzyme and pathway databases

BRENDAi2.3.1.5. 3474.
ReactomeiREACT_343875. Acetylation.

Miscellaneous databases

NextBioi292883.
PROiP50294.
SOURCEiSearch...

Gene expression databases

BgeeiP50294.
ExpressionAtlasiP50294. baseline and differential.
GenevisibleiP50294. MM.

Family and domain databases

InterProiIPR001447. Arylamine_N-AcTrfase.
[Graphical view]
PANTHERiPTHR11786. PTHR11786. 1 hit.
PfamiPF00797. Acetyltransf_2. 1 hit.
[Graphical view]
PRINTSiPR01543. ANATRNSFRASE.
ProtoNetiSearch...

Publicationsi

  1. "Molecular genetic basis of rapid and slow acetylation in mice."
    Martell K.J., Vatsis K.P., Weber W.W.
    Mol. Pharmacol. 40:218-227(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6.
  2. Hein D.W., Doll M.A.
    Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C3H/HeJ.
    Tissue: Heart.
  3. "Arylamine N-acetyltransferase in Balb/c mice: identification of a novel mouse isoenzyme by cloning and expression in vitro."
    Kelly S.L., Sim E.
    Biochem. J. 302:347-353(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE, CHARACTERIZATION.
    Strain: BALB/c.
    Tissue: Liver.
  4. "Cloned mouse N-acetyltransferases: enzymatic properties of expressed Nat-1 and Nat-2 gene products."
    Martell K.J., Levy G.N., Weber W.W.
    Mol. Pharmacol. 42:265-272(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiARY1_MOUSE
AccessioniPrimary (citable) accession number: P50294
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 24, 2015
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.