Reviewed, UniProtKB/Swiss-Prot P50293 (ARY2_MESAU)

Names and origin

Protein names

Recommended name:
    Arylamine N-acetyltransferase 2
    EC=2.3.1.5
Alternative name(s):
    Arylamide acetylase 2
    Polymorphic arylamine N-acetyltransferase
      Short name=PNAT
    N-acetyltransferase type 2
      Short name=NAT-2
      Short name=AT-2

Gene names

Name:	NAT2
Synonyms:	AAC2

Organism

Mesocricetus auratus (Golden hamster)

Taxonomic identifier

10036 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Mesocricetus

Protein attributes

Sequence length	290 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Participates in the detoxification of a plethora of hydrazine and arylamine drugs.
Catalytic activity	Acetyl-CoA + an arylamine = CoA + an N-acetylarylamine.
Subcellular location	Cytoplasm.
Polymorphism	There are two forms of NAT2: a rapid isoform and a slow isoform.
Sequence similarities	Belongs to the arylamine N-acetyltransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Molecular function	Acyltransferase Transferase
PTM	Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	arylamine N-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 290	290	Arylamine N-acetyltransferase 2		PRO_0000107907
Regions
	Region	106 – 107	2	Substrate binding By similarity
Sites
	Active site	68	1	Acyl-thioester intermediate By similarity
	Active site	107	1	By similarity
	Active site	122	1	By similarity
	Binding site	103	1	Coenzyme A By similarity
	Binding site	104	1	Coenzyme A; via amide nitrogen By similarity
	Binding site	208	1	Coenzyme A By similarity
Amino acid modifications
	Modified residue	246	1	Phosphotyrosine By similarity
Natural variations
	Natural variant	243 – 290	48	Missing in slow isoform.

Sequences

	Sequence		Length	Mass (Da)
	P50293-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 54C10F5F6990F6FD	FASTA	290	33,833
	10 20 30 40 50 60 MDIEAYFERI GYQNSRNKLD LQTLTEILQH QIRAIPFENL NIHCGESMEL SLETIFDQIV 70 80 90 100 110 120 RKKRGGWCLQ VNHLLYWALT KMGFETTMLG GYVFNTPANK YSSGMIHLLV QVTISDRNYI 130 140 150 160 170 180 VDAGFGRSLQ MWEPLELVSG KDHPQVPAIF RLTEENETWY LDQIRREQYV PNQAFVNSDL 190 200 210 220 230 240 LEKNKYRKIY SFTLEPRTIE DFESMNTYLQ TSPASVFTSK SFCSLQTPEG VHCLVGCTLT 250 260 270 280 290 YRRFSYKDNV DLVEFKSLKE EEIEDVLKTI FGISLEKKLV PKHGDRFFTI

References

[1]	"Polymorphic arylamine N-acetyltransferase encoding gene (NAT2) from homozygous rapid and slow acetylator congenic Syrian hamsters." Ferguson R.J., Doll M.A., Baumstark B.R., Hein D.W. Gene 140:247-249(1994) [PubMed: 8144033] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Heart.
[2]	"Biochemical and genetic analysis of two acetyltransferases from hamster tissues that can metabolize aromatic amine derivatives." Land S.J., Jones R.F., King C.M. Carcinogenesis 15:1585-1595(1994) [PubMed: 8055637] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Tissue: Liver.
[3]	"Primary structure and molecular basis of polymorphic appearance of an acetyltransferase (AT-II)* in hamsters." Nagata K., Ozawa S., Miyata M., Shimada M., Yamazoe Y., Kato R. Pharmacogenetics 4:91-100(1994) [PubMed: 7915937] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBLGenBankDDBJ

U03468 Genomic DNA. Translation: AAB60524.1.
U03467 Genomic DNA. Translation: AAB60523.1.
L24912 mRNA. Translation: AAA21829.1.
S72005 Genomic DNA. Translation: AAB31917.1.
S72007 Genomic DNA. Translation: AAB31918.1.

PIR

I78930.
I78931.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2GWV	model	-	A	1-290	[»]

SMR

P50293. Positions 2-290.

ModBase

Search...

Phylogenomic databases

HOVERGEN

P50293.

Enzyme and pathway databases

BRENDA

2.3.1.5. 824.
2.3.1.56. 824.

Family and domain databases

InterPro

IPR001447. N-AcTrfase.
[Graphical view]

PANTHER

PTHR11786. Acetyltransf2. 1 hit.

Pfam

PF00797. Acetyltransf_2. 1 hit.
[Graphical view]

PRINTS

PR01543. ANATRNSFRASE.

ProDom

PD002783. Acetyltransf2. 1 hit.
[Graphical view] [Entries sharing at least one domain]

ProtoNet

Search...

Entry information

Entry name

ARY2_MESAU

Accession

Primary (citable) accession number: P50293

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)