P50292 (ARY1_MESAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arylamine N-acetyltransferase 1
EC=2.3.1.5

Alternative name(s):
Arylamide acetylase 1
Monomorphic arylamine N-acetyltransferase
Short name=MNAT
N-acetyltransferase type 1
Short name=NAT-1

Gene names

Name:	NAT1
Synonyms:	AAC1

Organism

Mesocricetus auratus (Golden hamster)

Taxonomic identifier

10036 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Mesocricetus

Protein attributes

Sequence length	290 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Participates in the detoxification of a plethora of hydrazine and arylamine drugs.
Catalytic activity	Acetyl-CoA + an arylamine = CoA + an N-acetylarylamine.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the arylamine N-acetyltransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	arylamine N-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 290	290	Arylamine N-acetyltransferase 1		PRO_0000107906
Regions
	Region	106 – 107	2	Substrate binding By similarity
Sites
	Active site	68	1	Acyl-thioester intermediate By similarity
	Active site	107	1	By similarity
	Active site	122	1	By similarity
	Binding site	103	1	Coenzyme A By similarity
	Binding site	208	1	Coenzyme A By similarity
	Binding site	287	1	Coenzyme A By similarity

Sequences

	Sequence		Length	Mass (Da)
	P50292 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 06C26A0CCEACAF02	FASTA	290	33,500
	10 20 30 40 50 60 MDIEAYFERI GYNNPVYTLD LATLTEVLQH QMRTIPFENL NMHCGEAMDL GLEATFDQIV 70 80 90 100 110 120 RKKRGGWCLQ VNHLLYWALT QMGFETTMLG GYVYIVPVSK YSSEMIHLLV QVTISDRNYI 130 140 150 160 170 180 VDAAYGGSYQ MWEPVELASG KDQPQVPAIF RLTEENETWY LDQIRREQHV PNQEFVNSDL 190 200 210 220 230 240 LEKNTYRKIY SFTLQPRTIE DFEYANTYLQ ISPVSVFVNT SFCSLQTSEG VCCLIGSTIA 250 260 270 280 290 RRKFSYKENV DLVEFKNVSE EEIEDVLKTA FGVSLERKFV PKNGNLSFSI

References

[1]	"An arylamine acetyltransferase (AT-I) from Syrian golden hamster liver: cloning, complete nucleotide sequence, and expression in mammalian cells." Abu-Zeid M., Nagata K., Miyata M., Ozawa S., Fukuhara M., Yamazoe Y. Mol. Carcinog. 4:81-88(1991) [PubMed: 2009137] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"Syrian hamster monomorphic N-acetyltransferase (NAT1) alleles: amplification, cloning, sequencing, and expression in E. coli." Ferguson R.J., Doll M.A., Rustan T.D., Baumstark B.R., Hein D.W. Pharmacogenetics 4:82-90(1994) [PubMed: 8081415] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Heart.
[3]	"Primary structure and molecular basis of polymorphic appearance of an acetyltransferase (AT-II)* in hamsters." Nagata K., Ozawa S., Miyata M., Shimada M., Yamazoe Y., Kato R. Pharmacogenetics 4:91-100(1994) [PubMed: 7915937] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Hamster monomorphic arylamine N-acetyltransferase: expression in Escherichia coli and purification." Bergstrom C.P., Wagner C.R., Ann D.K., Hanna P.E. Protein Expr. Purif. 6:45-55(1995) [PubMed: 7756838] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X54142 mRNA. Translation: CAA38081.1. U05271 Genomic DNA. Translation: AAB60522.1. S72004 Genomic DNA. Translation: AAB31916.1.
PIR	A61397.
3D structure databases
ProteinModelPortal	P50292.
SMR	P50292. Positions 2-290.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG000165.
Enzyme and pathway databases
BRENDA	2.3.1.5. 3239.
Family and domain databases
InterPro	IPR001447. N-AcTrfase. [Graphical view]
PANTHER	PTHR11786. Acetyltransf2. 1 hit.
Pfam	PF00797. Acetyltransf_2. 1 hit. [Graphical view]
PRINTS	PR01543. ANATRNSFRASE.
ProtoNet	Search...

Entry information

Entry name

ARY1_MESAU

Accession

Primary (citable) accession number: P50292

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 31, 2011
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program