P50286 (ASPG_WOLSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
L-asparaginase
Short name=L-ASNase
EC=3.5.1.1

Alternative name(s):
L-asparagine amidohydrolase

Gene names

Name:	ansA
Synonyms:	ansB
Ordered Locus Names:	WS0660

Organism

Wolinella succinogenes [Complete proteome] [HAMAP]

Taxonomic identifier

844 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Wolinella

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	L-asparagine + H2O = L-aspartate + NH3.
Subunit structure	Homotetramer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the asparaginase 1 family.
Sequence caution	The sequence CAE09790.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	asparagine metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	asparaginase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 330

330

L-asparaginase

PRO_0000171086

Regions

Region

93 – 94

2

Substrate binding By similarity

Sites

Active site

14

1

O-isoaspartyl threonine intermediate Probable

Experimental info

Sequence conflict

121

1

S → P in CAA61503. Ref.1

Sequence conflict

121

1

S → P in CAA58658. Ref.1

Secondary structure

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330

Helix Strand Turn

Details...

Beta strand

5 – 13

9

Helix

32 – 40

9

Helix

42 – 46

5

Beta strand

49 – 54

6

Helix

60 – 62

3

Helix

65 – 80

16

Beta strand

87 – 90

4

Beta strand

93 – 95

3

Helix

96 – 106

11

Beta strand

113 – 116

4

Helix

129 – 140

12

Helix

143 – 145

3

Beta strand

150 – 154

5

Beta strand

157 – 160

4

Turn

161 – 163

3

Beta strand

183 – 187

5

Beta strand

190 – 193

4

Beta strand

195 – 197

3

Helix

202 – 204

3

Beta strand

218 – 222

5

Helix

230 – 237

8

Beta strand

241 – 248

8

Turn

249 – 251

3

Helix

255 – 266

12

Beta strand

270 – 280

11

Beta strand

284 – 288

5

Helix

290 – 293

4

Beta strand

295 – 297

3

Helix

303 – 314

12

Helix

320 – 327

8

Sequences

	Sequence		Length	Mass (Da)
	P50286 [UniParc]. Last modified September 26, 2003. Version 2. Checksum: B706B41958E924CE	FASTA	330	34,856
	10 20 30 40 50 60 MAKPQVTILA TGGTIAGSGE SSVKSSYSAG AVTVDKLLAA VPAINDLATI KGEQISSIGS 70 80 90 100 110 120 QEMTGKVWLK LAKRVNELLA QKETEAVIIT HGTDTMEETA FFLNLTVKSQ KPVVLVGAMR 130 140 150 160 170 180 SGSSMSADGP MNLYNAVNVA INKASTNKGV VIVMNDEIHA AREATKLNTT AVNAFASPNT 190 200 210 220 230 240 GKIGTVYYGK VEYFTQSVRP HTLASEFDIS KIEELPRVDI LYAHPDDTDV LVNAALQAGA 250 260 270 280 290 300 KGIIHAGMGN GNPFPLTQNA LEKAAKSGVV VARSSRVGSG STTQEAEVDD KKLGFVATES 310 320 330 LNPQKARVLL MLALTKTSDR EAIQKIFSTY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Crystal structure and amino acid sequence of Wolinella succinogenes L-asparaginase." Lubkowski J., Palm G.J., Gilliland G.L., Derst C., Roehm K.H., Wlodawer A. Eur. J. Biochem. 241:201-207(1996) [PubMed: 8898907] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
[2]	"Complete genome sequence and analysis of Wolinella succinogenes." Baar C., Eppinger M., Raddatz G., Simon J., Lanz C., Klimmek O., Nandakumar R., Gross R., Rosinus A., Keller H., Jagtap P., Linke B., Meyer F., Lederer H., Schuster S.C. Proc. Natl. Acad. Sci. U.S.A. 100:11690-11695(2003) [PubMed: 14500908] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSMZ 1740.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X89215 Genomic DNA. Translation: CAA61503.1.
X83689 Genomic DNA. Translation: CAA58658.1.
BX571658 Genomic DNA. Translation: CAE09790.1. Different initiation.

PIR

S74205.

RefSeq

NP_906890.1. NC_005090.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1WSA	X-ray	2.20	A/B	1-330	[»]

ProteinModelPortal

P50286.

SMR

P50286. Positions 3-330.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

2555338.

GenomeReviews

Gene locus WS0660 in contig BX571656_GR.

KEGG

wsu:WS0660.

NMPDR

fig|273121.1.peg.617.

PATRIC

24038396. VBIWolSuc63014_0628.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

HBG497495.

ProtClustDB

CLSK922838.

Enzyme and pathway databases

BioCyc

WSUC273121:WS0660-MONOMER.

Family and domain databases

InterPro

IPR004550. AsnASE_II.
IPR006034. Asparaginase/glutaminase.
IPR020827. Asparaginase/glutaminase_CS.
[Graphical view]

KO

K01424.

PANTHER

PTHR11707. Asp/Glutamnse. 1 hit.

Pfam

PF00710. Asparaginase. 1 hit.
[Graphical view]

PIRSF

PIRSF001220. L-ASNase_gatD. 1 hit.

PRINTS

PR00139. ASNGLNASE.

SMART

SM00870. Asparaginase. 1 hit.
[Graphical view]

SUPFAM

SSF53774. Asp/Glutamnse. 1 hit.

TIGRFAMs

TIGR00520. AsnASE_II. 1 hit.

PROSITE

PS00144. ASN_GLN_ASE_1. 1 hit.
PS00917. ASN_GLN_ASE_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ASPG_WOLSU

Accession

Primary (citable) accession number: P50286

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	September 26, 2003
Last modified:	January 25, 2012
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program