Reviewed, UniProtKB/Swiss-Prot P50285 (FMO1_MOUSE)

Names and origin

Protein names

Recommended name:
    Dimethylaniline monooxygenase [N-oxide-forming] 1
    EC=1.14.13.8
Alternative name(s):
    Hepatic flavin-containing monooxygenase 1
      Short name=FMO 1
    Dimethylaniline oxidase 1

Gene names

Name:	Fmo1
Synonyms:	Fmo-1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	532 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This protein is involved in the oxidative metabolism of a variety of xenobiotics such as drugs and pesticides. Form I catalyzes the N-oxygenation of secondary and tertiary amines.
Catalytic activity	N,N-dimethylaniline + NADPH + O2 = N,N-dimethylaniline N-oxide + NADP+ + H2O.
Cofactor	FAD.
Subcellular location	Microsome membrane. Endoplasmic reticulum membrane.
Tissue specificity	Liver By similarity.
Sequence similarities	Belongs to the FMO family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane
Ligand	FAD Flavoprotein NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW intrinsic to endoplasmic reticulum membrane Inferred from electronic annotation. Source: InterPro microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NADP or NADPH binding Inferred from electronic annotation. Source: InterPro flavin-containing monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 532	532	Dimethylaniline monooxygenase [N-oxide-forming] 1		PRO_0000147640
Regions
	Nucleotide binding	9 – 14	6	FAD Potential
	Nucleotide binding	191 – 196	6	NADP Potential
Sites
	Site	208	1	Important for substrate binding By similarity

Sequences

	Sequence		Length	Mass (Da)
	P50285-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: C3CEDC7FC82DAC59	FASTA	532	59,915
	10 20 30 40 50 60 MVKRVAIVGA GVSGLASIKC CLEEGLEPTC FERSSDLGGL WRFTEHVEEG RASLYKSVVS 70 80 90 100 110 120 NSSREMSCYP DFPFPEDYPN FVPNSLFLEY LKLYSTQFNL QRCIYFNTKV CSITKRPDFA 130 140 150 160 170 180 VSGQWEVVTV TNGKQNSAIF DAVMVCTGFL TNPHLPLDSF PGILTFKGEY FHSRQYKHPD 190 200 210 220 230 240 IFKDKRVLVV GMGNSGTDIA VEASHLAKKV FLSTTGGAWV ISRVFDSGYP WDMIFMTRFQ 250 260 270 280 290 300 NMLRNLLPTP IVSWLISKKM NSWFNHVNYG VAPEDRTQLR EPVLNDELPG RIITGKVFIK 310 320 330 340 350 360 PSIKEVKENS VVFNNTPKEE PIDIIVFATG YTFAFPFLDE SVVKVEDGQA SLYKYIFPAH 370 380 390 400 410 420 LPKPTLAVIG LIKPLGSMVP TGETQARWVV QVLKGATTLP PPSVMMEEVN ERKKNKHSGF 430 440 450 460 470 480 GLCYCKALQT DYITYIDDLL TSINAKPDLR AMLLTDPRLA LSIFFGPCTP YHFRLTGPGK 490 500 510 520 530 WEGARKAILT QWDRTVKVTK TRTIQESPSS FETLLKLFSF LALLIAVFLI FL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Itoh K., Nakamura K., Kimura T., Itoh S., Kamataki T. Submitted (MAY-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Molecular cloning, sequence, and expression of mouse flavin-containing monooxygenases 1 and 5 (FMO1 and FMO5)." Cherrington N.J., Falls J.G., Rose R.L., Clements K.M., Philpot R.M., Levi P.E., Hodgson E. J. Biochem. Mol. Toxicol. 12:205-212(1998) [PubMed: 9580872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: CD-1. Tissue: Liver.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Liver.
[4]	Lubec G., Sunyer B., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 297-307, MASS SPECTROMETRY. Strain: OF1. Tissue: Hippocampus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D16215 mRNA. Translation: BAA03745.1. U87456 mRNA. Translation: AAB47569.1. BC011229 mRNA. Translation: AAH11229.1.
IPI	IPI00116432.
PIR	B61243.
RefSeq	NP_034361.1.
UniGene	Mm.976
3D structure databases
SMR	P50285. Positions 1-467.
ModBase	Search...
Protein-protein interaction databases
STRING	P50285.
PTM databases
PhosphoSite	P50285.
Proteomic databases
PRIDE	P50285.
Genome annotation databases
Ensembl	ENSMUST00000046049; ENSMUSP00000037259; ENSMUSG00000040181; Mus musculus. [Genome view]
GeneID	14261.
KEGG	mmu:14261.
UCSC	uc007dgx.1. mouse.
Organism-specific databases
CTD	14261.
MGI	MGI:1310002. Fmo1.
Phylogenomic databases
eggNOG	roNOG11485.
HOGENOM	HBG700572.
HOVERGEN	P50285.
InParanoid	P50285.
OMA	MLLTDPR.
OrthoDB	EOG9F7R4X.
PhylomeDB	P50285.
Enzyme and pathway databases
BRENDA	1.14.13.8. 244.
Gene expression databases
ArrayExpress	P50285.
Bgee	P50285.
CleanEx	MM_FMO1.
Genevestigator	P50285.
GermOnline	ENSMUSG00000040181. Mus musculus.
Family and domain databases
InterPro	IPR012143. dManiline_mOase. IPR000960. Flavin_mOase. IPR020946. Flavin_mOase-like. IPR002253. Flavin_mOase_1. [Graphical view]
Pfam	PF00743. FMO-like. 1 hit. [Graphical view]
PIRSF	PIRSF000332. FMO. 1 hit.
PRINTS	PR00370. FMOXYGENASE. PR01121. FMOXYGENASE1.
ProtoNet	Search...
Other Resources
NextBio	285603.
SOURCE	Search...

Entry information

Entry name

FMO1_MOUSE

Accession

Primary (citable) accession number: P50285

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	January 19, 2010
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)