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Protein

Matrilysin

Gene

Mmp7

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase (By similarity).By similarity

Catalytic activityi

Cleavage of 14-Ala-|-Leu-15 and 16-Tyr-|-Leu-17 in B chain of insulin. No action on collagen types I, II, IV, V. Cleaves gelatin chain alpha-2(I) > alpha-1(I).

Cofactori

Protein has several cofactor binding sites:
  • Ca2+By similarityNote: Binds 2 calcium ions per subunit.By similarity
  • Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi90Zinc 2; in inhibited formBy similarity1
Metal bindingi156Calcium 1By similarity1
Metal bindingi166Zinc 1By similarity1
Metal bindingi168Zinc 1By similarity1
Metal bindingi173Calcium 2By similarity1
Metal bindingi174Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi176Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi178Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi181Zinc 1By similarity1
Metal bindingi188Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi190Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi192Calcium 1By similarity1
Metal bindingi194Zinc 1By similarity1
Metal bindingi196Calcium 2By similarity1
Metal bindingi199Calcium 2By similarity1
Metal bindingi217Zinc 2; catalyticBy similarity1
Active sitei218PROSITE-ProRule annotation1
Metal bindingi221Zinc 2; catalyticBy similarity1
Metal bindingi227Zinc 2; catalyticBy similarity1

GO - Molecular functioni

  • heparin binding Source: RGD
  • metalloendopeptidase activity Source: InterPro
  • zinc ion binding Source: InterPro

GO - Biological processi

  • aging Source: RGD
  • cellular response to mechanical stimulus Source: RGD
  • collagen catabolic process Source: UniProtKB-KW
  • estrous cycle Source: RGD
  • maternal process involved in female pregnancy Source: RGD
  • response to nutrient levels Source: RGD

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
Biological processCollagen degradation
LigandCalcium, Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.4.24.23 5301
ReactomeiR-RNO-1442490 Collagen degradation
R-RNO-1474228 Degradation of the extracellular matrix
R-RNO-1592389 Activation of Matrix Metalloproteinases
R-RNO-2022090 Assembly of collagen fibrils and other multimeric structures

Protein family/group databases

MEROPSiM10.008

Names & Taxonomyi

Protein namesi
Recommended name:
Matrilysin (EC:3.4.24.23)
Alternative name(s):
Matrin
Matrix metalloproteinase-7
Short name:
MMP-7
Pump-1 protease
Uterine metalloproteinase
Gene namesi
Name:Mmp7
Synonyms:Mmp-7
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi3100 Mmp7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20By similarityAdd BLAST20
PropeptideiPRO_000002874221 – 97Activation peptideBy similarityAdd BLAST77
ChainiPRO_000002874398 – 267MatrilysinAdd BLAST170

Keywords - PTMi

Zymogen

Proteomic databases

PaxDbiP50280
PRIDEiP50280

PTM databases

PhosphoSitePlusiP50280

Expressioni

Gene expression databases

BgeeiENSRNOG00000010507
GenevisibleiP50280 RN

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000014041

Structurei

3D structure databases

ProteinModelPortaliP50280
SMRiP50280
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi88 – 95Cysteine switchBy similarity8

Domaini

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Sequence similaritiesi

Belongs to the peptidase M10A family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1565 Eukaryota
ENOG410XQ5D LUCA
GeneTreeiENSGT00760000118870
HOGENOMiHOG000239471
HOVERGENiHBG052484
InParanoidiP50280
KOiK01397
OMAiYPTYGNG
OrthoDBiEOG091G03DP
PhylomeDBiP50280
TreeFamiTF315428

Family and domain databases

CDDicd04278 ZnMc_MMP, 1 hit
Gene3Di3.40.390.10, 1 hit
InterProiView protein in InterPro
IPR033739 M10A_MMP
IPR028707 Matrilysin
IPR024079 MetalloPept_cat_dom_sf
IPR001818 Pept_M10_metallopeptidase
IPR021190 Pept_M10A
IPR021158 Pept_M10A_Zn_BS
IPR006026 Peptidase_Metallo
IPR002477 Peptidoglycan-bd-like
IPR036365 PGBD-like_sf
PANTHERiPTHR10201:SF143 PTHR10201:SF143, 1 hit
PfamiView protein in Pfam
PF00413 Peptidase_M10, 1 hit
PF01471 PG_binding_1, 1 hit
PRINTSiPR00138 MATRIXIN
SMARTiView protein in SMART
SM00235 ZnMc, 1 hit
SUPFAMiSSF47090 SSF47090, 1 hit
PROSITEiView protein in PROSITE
PS00546 CYSTEINE_SWITCH, 1 hit
PS00142 ZINC_PROTEASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50280-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAMRLTLFR IVCLLPGCLA LPLSQEAGEV TALQWEQAQN YLRKFYLHDS
60 70 80 90 100
KTKKATSAVD KLREMQKFFG LPETGKLSPR VMEIMQKPRC GVPDVAEFSL
110 120 130 140 150
MPNSPKWHSR TVTYRIVSYT TDLPRFLVDQ IVKRALRMWS MQIPLNFKRV
160 170 180 190 200
SWGTADIIIG FARGDHGDNF PFDGPGNTLG HAFAPGPGLG GDAHFDKDEY
210 220 230 240 250
WTDGEDSGVN FLFVATHELG HSLGLGHSSV PSSVMYPTYQ GDHSEDFSLT
260
KDDIAGIQKL YGKRNKL
Length:267
Mass (Da):29,885
Last modified:October 1, 1996 - v1
Checksum:iEBA3C3D9527A4C7B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L24374 mRNA Translation: AAA99432.1
BC064657 mRNA Translation: AAH64657.1
PIRiA57490
RefSeqiNP_036996.1, NM_012864.2
UniGeneiRn.10282

Genome annotation databases

EnsembliENSRNOT00000014041; ENSRNOP00000014041; ENSRNOG00000010507
GeneIDi25335
KEGGirno:25335
UCSCiRGD:3100 rat

Similar proteinsi

Entry informationi

Entry nameiMMP7_RAT
AccessioniPrimary (citable) accession number: P50280
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

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