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Protein

Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene

BIO3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of the alpha-amino group from S-adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form 7,8-diaminopelargonic acid (DAPA). It is the only animotransferase known to utilize SAM as an amino donor.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + 8-amino-7-oxononanoate = S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate.

Cofactori

Pathwayi: biotin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 7,8-diaminononanoate from 8-amino-7-oxononanoate (SAM route).
Proteins known to be involved in this subpathway in this organism are:
  1. Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (BIO3)
This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 7,8-diaminononanoate from 8-amino-7-oxononanoate (SAM route), the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei267-keto-8-aminopelargonic acidBy similarity1
Binding sitei270Pyridoxal phosphateBy similarity1
Binding sitei3147-keto-8-aminopelargonic acidBy similarity1
Binding sitei3507-keto-8-aminopelargonic acid; via carbonyl oxygenBy similarity1
Binding sitei352Pyridoxal phosphateBy similarity1
Binding sitei4417-keto-8-aminopelargonic acidBy similarity1

GO - Molecular functioni

GO - Biological processi

  • biotin biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

Pyridoxal phosphate, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciYEAST:YNR058W-MONOMER.
UniPathwayiUPA00078; UER00160.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC:2.6.1.62)
Alternative name(s):
7,8-diamino-pelargonic acid aminotransferase
Short name:
DAPA AT
Short name:
DAPA aminotransferase
Diaminopelargonic acid synthase
Gene namesi
Name:BIO3
Ordered Locus Names:YNR058W
ORF Names:N3510
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNR058W.
SGDiS000005341. BIO3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • mitochondrion Source: GO_Central
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001203781 – 480Adenosylmethionine-8-amino-7-oxononanoate aminotransferaseAdd BLAST480

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei314N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PRIDEiP50277.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi35884. 9 interactors.
DIPiDIP-4822N.
IntActiP50277. 1 interactor.
MINTiMINT-562496.

Structurei

3D structure databases

ProteinModelPortaliP50277.
SMRiP50277.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni65 – 667-keto-8-aminopelargonic acid bindingBy similarity2
Regioni126 – 127Pyridoxal phosphate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000020209.
InParanoidiP50277.
KOiK00833.
OMAiVHEGACE.
OrthoDBiEOG092C26XW.

Family and domain databases

CDDicd00610. OAT_like. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 2 hits.
InterProiIPR005814. Aminotrans_3.
IPR005815. BioA.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11986. PTHR11986. 2 hits.
PfamiPF00202. Aminotran_3. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR00508. bioA. 1 hit.

Sequencei

Sequence statusi: Complete.

P50277-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQEISYTPD VAELLDFDKK HIWHPYTSLS SPLNVYPVKS AHGCKLVLDT
60 70 80 90 100
DSPVDVEVID AMSSWWCVIH GYNNPELNEA LTKQMLKFSH VLLGGFTHKG
110 120 130 140 150
AVNLVQKLLK VIDEPSLQYC FLADSGSVAV EVALKMALQS NMSGEATKNR
160 170 180 190 200
TKFLTIKNGY HGDTFGAMSV CDPENSMHHI YNDRLSENIF AQAPSIVDGL
210 220 230 240 250
PTSQNGFEDH WNAEEVTDLK KQFELHSDKI CAVILEPILQ GAGGLRPYHP
260 270 280 290 300
QFLIEVQKLC NQYDVLFIMD EIATGFGRTG EIFAFKHCQK YQDQHGISPS
310 320 330 340 350
DQIKVVPDIL CVGKGLTSGY MTMSAVVVND KVASRISSPN SPTGGCFMHG
360 370 380 390 400
PTFMGNALAC SVAEKSMDIL LRGEWRKQVS AIENQIYREL YQYIKNPDNG
410 420 430 440 450
LIGTVVKRVS VIGAVGIVEL YKKTDPEWFQ KKFISKGVHI RPFNCLCYIM
460 470 480
PPYVITTEEL TKVNQVLIEV LHEWKSHINQ
Length:480
Mass (Da):53,709
Last modified:October 1, 1996 - v1
Checksum:i00A8BC42DCBED19A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti140S → P in AAU09779 (PubMed:17322287).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti229K → E in strain: ATCC 28383 and ATCC 204626. 1 Publication1
Natural varianti295H → R in strain: ATCC 28383 and ATCC 204626. 1 Publication1
Natural varianti384N → D in strain: ATCC 28383 and ATCC 204626. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U47112 Genomic DNA. Translation: AAA88905.1.
U53467 Genomic DNA. Translation: AAB63970.1.
AB200248 Genomic DNA. Translation: BAE00005.1.
Z71673 Genomic DNA. Translation: CAA96340.1.
AY723862 Genomic DNA. Translation: AAU09779.1.
BK006947 Genomic DNA. Translation: DAA10599.1.
PIRiS63390.
RefSeqiNP_014456.1. NM_001183235.1.

Genome annotation databases

EnsemblFungiiYNR058W; YNR058W; YNR058W.
GeneIDi855795.
KEGGisce:YNR058W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U47112 Genomic DNA. Translation: AAA88905.1.
U53467 Genomic DNA. Translation: AAB63970.1.
AB200248 Genomic DNA. Translation: BAE00005.1.
Z71673 Genomic DNA. Translation: CAA96340.1.
AY723862 Genomic DNA. Translation: AAU09779.1.
BK006947 Genomic DNA. Translation: DAA10599.1.
PIRiS63390.
RefSeqiNP_014456.1. NM_001183235.1.

3D structure databases

ProteinModelPortaliP50277.
SMRiP50277.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35884. 9 interactors.
DIPiDIP-4822N.
IntActiP50277. 1 interactor.
MINTiMINT-562496.

Proteomic databases

PRIDEiP50277.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNR058W; YNR058W; YNR058W.
GeneIDi855795.
KEGGisce:YNR058W.

Organism-specific databases

EuPathDBiFungiDB:YNR058W.
SGDiS000005341. BIO3.

Phylogenomic databases

HOGENOMiHOG000020209.
InParanoidiP50277.
KOiK00833.
OMAiVHEGACE.
OrthoDBiEOG092C26XW.

Enzyme and pathway databases

UniPathwayiUPA00078; UER00160.
BioCyciYEAST:YNR058W-MONOMER.

Miscellaneous databases

PROiP50277.

Family and domain databases

CDDicd00610. OAT_like. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 2 hits.
InterProiIPR005814. Aminotrans_3.
IPR005815. BioA.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11986. PTHR11986. 2 hits.
PfamiPF00202. Aminotran_3. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR00508. bioA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBIOA_YEAST
AccessioniPrimary (citable) accession number: P50277
Secondary accession number(s): D6W1N3, E9P967, Q4R1J0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 149 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.