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P50277

- BIOA_YEAST

UniProt

P50277 - BIOA_YEAST

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Protein

Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene

BIO3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of the alpha-amino group from S-adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form 7,8-diaminopelargonic acid (DAPA). It is the only animotransferase known to utilize SAM as an amino donor.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + 8-amino-7-oxononanoate = S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate.

Cofactori

Pyridoxal phosphate.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei26 – 2617-keto-8-aminopelargonic acidBy similarity
Binding sitei270 – 2701Pyridoxal phosphateBy similarity
Binding sitei314 – 31417-keto-8-aminopelargonic acidBy similarity
Binding sitei350 – 35017-keto-8-aminopelargonic acid; via carbonyl oxygenBy similarity
Binding sitei352 – 3521Pyridoxal phosphateBy similarity
Binding sitei441 – 44117-keto-8-aminopelargonic acidBy similarity

GO - Molecular functioni

  1. adenosylmethionine-8-amino-7-oxononanoate transaminase activity Source: SGD
  2. pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  1. biotin biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

Pyridoxal phosphate, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciYEAST:YNR058W-MONOMER.
UniPathwayiUPA00078; UER00160.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC:2.6.1.62)
Alternative name(s):
7,8-diamino-pelargonic acid aminotransferase
Short name:
DAPA AT
Short name:
DAPA aminotransferase
Diaminopelargonic acid synthase
Gene namesi
Name:BIO3
Ordered Locus Names:YNR058W
ORF Names:N3510
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNR058w.
SGDiS000005341. BIO3.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 480480Adenosylmethionine-8-amino-7-oxononanoate aminotransferasePRO_0000120378Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei314 – 3141N6-(pyridoxal phosphate)lysineBy similarity

Expressioni

Gene expression databases

GenevestigatoriP50277.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
MSH3P253361EBI-3632,EBI-11362

Protein-protein interaction databases

BioGridi35884. 9 interactions.
DIPiDIP-4822N.
IntActiP50277. 1 interaction.
MINTiMINT-562496.

Structurei

3D structure databases

ProteinModelPortaliP50277.
SMRiP50277. Positions 15-463.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni65 – 6627-keto-8-aminopelargonic acid bindingBy similarity
Regioni126 – 1272Pyridoxal phosphate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0161.
HOGENOMiHOG000020209.
InParanoidiP50277.
KOiK00833.
OMAiMATMAIC.
OrthoDBiEOG7Q2NFK.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 2 hits.
InterProiIPR005814. Aminotrans_3.
IPR005815. BioA.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11986. PTHR11986. 1 hit.
PfamiPF00202. Aminotran_3. 1 hit.
[Graphical view]
PIRSFiPIRSF000521. Transaminase_4ab_Lys_Orn. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR00508. bioA. 1 hit.

Sequencei

Sequence statusi: Complete.

P50277-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSQEISYTPD VAELLDFDKK HIWHPYTSLS SPLNVYPVKS AHGCKLVLDT
60 70 80 90 100
DSPVDVEVID AMSSWWCVIH GYNNPELNEA LTKQMLKFSH VLLGGFTHKG
110 120 130 140 150
AVNLVQKLLK VIDEPSLQYC FLADSGSVAV EVALKMALQS NMSGEATKNR
160 170 180 190 200
TKFLTIKNGY HGDTFGAMSV CDPENSMHHI YNDRLSENIF AQAPSIVDGL
210 220 230 240 250
PTSQNGFEDH WNAEEVTDLK KQFELHSDKI CAVILEPILQ GAGGLRPYHP
260 270 280 290 300
QFLIEVQKLC NQYDVLFIMD EIATGFGRTG EIFAFKHCQK YQDQHGISPS
310 320 330 340 350
DQIKVVPDIL CVGKGLTSGY MTMSAVVVND KVASRISSPN SPTGGCFMHG
360 370 380 390 400
PTFMGNALAC SVAEKSMDIL LRGEWRKQVS AIENQIYREL YQYIKNPDNG
410 420 430 440 450
LIGTVVKRVS VIGAVGIVEL YKKTDPEWFQ KKFISKGVHI RPFNCLCYIM
460 470 480
PPYVITTEEL TKVNQVLIEV LHEWKSHINQ
Length:480
Mass (Da):53,709
Last modified:October 1, 1996 - v1
Checksum:i00A8BC42DCBED19A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti140 – 1401S → P in AAU09779. (PubMed:17322287)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti229 – 2291K → E in strain: ATCC 28383 and ATCC 204626. 1 Publication
Natural varianti295 – 2951H → R in strain: ATCC 28383 and ATCC 204626. 1 Publication
Natural varianti384 – 3841N → D in strain: ATCC 28383 and ATCC 204626. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U47112 Genomic DNA. Translation: AAA88905.1.
U53467 Genomic DNA. Translation: AAB63970.1.
AB200248 Genomic DNA. Translation: BAE00005.1.
Z71673 Genomic DNA. Translation: CAA96340.1.
AY723862 Genomic DNA. Translation: AAU09779.1.
BK006947 Genomic DNA. Translation: DAA10599.1.
PIRiS63390.
RefSeqiNP_014456.1. NM_001183235.1.

Genome annotation databases

EnsemblFungiiYNR058W; YNR058W; YNR058W.
GeneIDi855795.
KEGGisce:YNR058W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U47112 Genomic DNA. Translation: AAA88905.1 .
U53467 Genomic DNA. Translation: AAB63970.1 .
AB200248 Genomic DNA. Translation: BAE00005.1 .
Z71673 Genomic DNA. Translation: CAA96340.1 .
AY723862 Genomic DNA. Translation: AAU09779.1 .
BK006947 Genomic DNA. Translation: DAA10599.1 .
PIRi S63390.
RefSeqi NP_014456.1. NM_001183235.1.

3D structure databases

ProteinModelPortali P50277.
SMRi P50277. Positions 15-463.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35884. 9 interactions.
DIPi DIP-4822N.
IntActi P50277. 1 interaction.
MINTi MINT-562496.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNR058W ; YNR058W ; YNR058W .
GeneIDi 855795.
KEGGi sce:YNR058W.

Organism-specific databases

CYGDi YNR058w.
SGDi S000005341. BIO3.

Phylogenomic databases

eggNOGi COG0161.
HOGENOMi HOG000020209.
InParanoidi P50277.
KOi K00833.
OMAi MATMAIC.
OrthoDBi EOG7Q2NFK.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00160 .
BioCyci YEAST:YNR058W-MONOMER.

Miscellaneous databases

NextBioi 980291.
PROi P50277.

Gene expression databases

Genevestigatori P50277.

Family and domain databases

Gene3Di 3.40.640.10. 1 hit.
3.90.1150.10. 2 hits.
InterProi IPR005814. Aminotrans_3.
IPR005815. BioA.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view ]
PANTHERi PTHR11986. PTHR11986. 1 hit.
Pfami PF00202. Aminotran_3. 1 hit.
[Graphical view ]
PIRSFi PIRSF000521. Transaminase_4ab_Lys_Orn. 1 hit.
SUPFAMi SSF53383. SSF53383. 1 hit.
TIGRFAMsi TIGR00508. bioA. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the biotin biosynthesis pathway in Saccharomyces cerevisiae and evidence for a cluster containing BIO5, a novel gene involved in vitamer uptake."
    Phalip V., Kuhn I., Lemoine Y., Jeltsch J.-M.
    Gene 232:43-51(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: ATCC 28383 / FL100 / VTT C-80102.
  2. "Identification and characterization of a novel biotin biosynthesis gene in Saccharomyces cerevisiae."
    Wu H., Ito K., Shimoi H.
    Appl. Environ. Microbiol. 71:6845-6855(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS GLU-229; ARG-295 AND ASP-384.
    Strain: ATCC 204626 / S288c / A364A.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.

Entry informationi

Entry nameiBIOA_YEAST
AccessioniPrimary (citable) accession number: P50277
Secondary accession number(s): D6W1N3, E9P967, Q4R1J0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3