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Protein

Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene

BIO3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the transfer of the alpha-amino group from S-adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form 7,8-diaminopelargonic acid (DAPA). It is the only animotransferase known to utilize SAM as an amino donor.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + 8-amino-7-oxononanoate = S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate.

Cofactori

Pathwayi: biotin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 7,8-diaminononanoate from 8-amino-7-oxononanoate (SAM route).
Proteins known to be involved in this subpathway in this organism are:
  1. Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (BIO3)
This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 7,8-diaminononanoate from 8-amino-7-oxononanoate (SAM route), the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei267-keto-8-aminopelargonic acidBy similarity1
Binding sitei270Pyridoxal phosphateBy similarity1
Binding sitei3147-keto-8-aminopelargonic acidBy similarity1
Binding sitei3507-keto-8-aminopelargonic acid; via carbonyl oxygenBy similarity1
Binding sitei352Pyridoxal phosphateBy similarity1
Binding sitei4417-keto-8-aminopelargonic acidBy similarity1

GO - Molecular functioni

GO - Biological processi

  • biotin biosynthetic process Source: SGD

Keywordsi

Molecular functionAminotransferase, Transferase
Biological processBiotin biosynthesis
LigandPyridoxal phosphate, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciYEAST:YNR058W-MONOMER
UniPathwayiUPA00078; UER00160

Names & Taxonomyi

Protein namesi
Recommended name:
Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC:2.6.1.62)
Alternative name(s):
7,8-diamino-pelargonic acid aminotransferase
Short name:
DAPA AT
Short name:
DAPA aminotransferase
Diaminopelargonic acid synthase
Gene namesi
Name:BIO3
Ordered Locus Names:YNR058W
ORF Names:N3510
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNR058W
SGDiS000005341 BIO3

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001203781 – 480Adenosylmethionine-8-amino-7-oxononanoate aminotransferaseAdd BLAST480

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei314N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PaxDbiP50277
PRIDEiP50277

Interactioni

Protein-protein interaction databases

BioGridi35884, 19 interactors
DIPiDIP-4822N
IntActiP50277, 8 interactors
MINTiP50277
STRINGi4932.YNR058W

Structurei

3D structure databases

ProteinModelPortaliP50277
SMRiP50277
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni65 – 667-keto-8-aminopelargonic acid bindingBy similarity2
Regioni126 – 127Pyridoxal phosphate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000020209
InParanoidiP50277
KOiK00833
OMAiFGGITHD
OrthoDBiEOG092C26XW

Family and domain databases

CDDicd00610 OAT_like, 1 hit
Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 2 hits
InterProiView protein in InterPro
IPR005814 Aminotrans_3
IPR005815 BioA
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PfamiView protein in Pfam
PF00202 Aminotran_3, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
TIGRFAMsiTIGR00508 bioA, 1 hit

Sequencei

Sequence statusi: Complete.

P50277-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQEISYTPD VAELLDFDKK HIWHPYTSLS SPLNVYPVKS AHGCKLVLDT
60 70 80 90 100
DSPVDVEVID AMSSWWCVIH GYNNPELNEA LTKQMLKFSH VLLGGFTHKG
110 120 130 140 150
AVNLVQKLLK VIDEPSLQYC FLADSGSVAV EVALKMALQS NMSGEATKNR
160 170 180 190 200
TKFLTIKNGY HGDTFGAMSV CDPENSMHHI YNDRLSENIF AQAPSIVDGL
210 220 230 240 250
PTSQNGFEDH WNAEEVTDLK KQFELHSDKI CAVILEPILQ GAGGLRPYHP
260 270 280 290 300
QFLIEVQKLC NQYDVLFIMD EIATGFGRTG EIFAFKHCQK YQDQHGISPS
310 320 330 340 350
DQIKVVPDIL CVGKGLTSGY MTMSAVVVND KVASRISSPN SPTGGCFMHG
360 370 380 390 400
PTFMGNALAC SVAEKSMDIL LRGEWRKQVS AIENQIYREL YQYIKNPDNG
410 420 430 440 450
LIGTVVKRVS VIGAVGIVEL YKKTDPEWFQ KKFISKGVHI RPFNCLCYIM
460 470 480
PPYVITTEEL TKVNQVLIEV LHEWKSHINQ
Length:480
Mass (Da):53,709
Last modified:October 1, 1996 - v1
Checksum:i00A8BC42DCBED19A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti140S → P in AAU09779 (PubMed:17322287).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti229K → E in strain: ATCC 28383 and ATCC 204626. 1 Publication1
Natural varianti295H → R in strain: ATCC 28383 and ATCC 204626. 1 Publication1
Natural varianti384N → D in strain: ATCC 28383 and ATCC 204626. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U47112 Genomic DNA Translation: AAA88905.1
U53467 Genomic DNA Translation: AAB63970.1
AB200248 Genomic DNA Translation: BAE00005.1
Z71673 Genomic DNA Translation: CAA96340.1
AY723862 Genomic DNA Translation: AAU09779.1
BK006947 Genomic DNA Translation: DAA10599.1
PIRiS63390
RefSeqiNP_014456.1, NM_001183235.1

Genome annotation databases

EnsemblFungiiYNR058W; YNR058W; YNR058W
GeneIDi855795
KEGGisce:YNR058W

Similar proteinsi

Entry informationi

Entry nameiBIOA_YEAST
AccessioniPrimary (citable) accession number: P50277
Secondary accession number(s): D6W1N3, E9P967, Q4R1J0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 28, 2018
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health