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P50277

- BIOA_YEAST

UniProt

P50277 - BIOA_YEAST

Protein

Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene

BIO3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Catalyzes the transfer of the alpha-amino group from S-adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form 7,8-diaminopelargonic acid (DAPA). It is the only animotransferase known to utilize SAM as an amino donor.1 Publication

    Catalytic activityi

    S-adenosyl-L-methionine + 8-amino-7-oxononanoate = S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate.

    Cofactori

    Pyridoxal phosphate.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei26 – 2617-keto-8-aminopelargonic acidBy similarity
    Binding sitei270 – 2701Pyridoxal phosphateBy similarity
    Binding sitei314 – 31417-keto-8-aminopelargonic acidBy similarity
    Binding sitei350 – 35017-keto-8-aminopelargonic acid; via carbonyl oxygenBy similarity
    Binding sitei352 – 3521Pyridoxal phosphateBy similarity
    Binding sitei441 – 44117-keto-8-aminopelargonic acidBy similarity

    GO - Molecular functioni

    1. adenosylmethionine-8-amino-7-oxononanoate transaminase activity Source: SGD
    2. pyridoxal phosphate binding Source: InterPro

    GO - Biological processi

    1. biotin biosynthetic process Source: SGD

    Keywords - Molecular functioni

    Aminotransferase, Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    Pyridoxal phosphate, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciYEAST:YNR058W-MONOMER.
    UniPathwayiUPA00078; UER00160.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC:2.6.1.62)
    Alternative name(s):
    7,8-diamino-pelargonic acid aminotransferase
    Short name:
    DAPA AT
    Short name:
    DAPA aminotransferase
    Diaminopelargonic acid synthase
    Gene namesi
    Name:BIO3
    Ordered Locus Names:YNR058W
    ORF Names:N3510
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNR058w.
    SGDiS000005341. BIO3.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 480480Adenosylmethionine-8-amino-7-oxononanoate aminotransferasePRO_0000120378Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei314 – 3141N6-(pyridoxal phosphate)lysineBy similarity

    Expressioni

    Gene expression databases

    GenevestigatoriP50277.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    MSH3P253361EBI-3632,EBI-11362

    Protein-protein interaction databases

    BioGridi35884. 9 interactions.
    DIPiDIP-4822N.
    IntActiP50277. 1 interaction.
    MINTiMINT-562496.

    Structurei

    3D structure databases

    ProteinModelPortaliP50277.
    SMRiP50277. Positions 15-473.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni65 – 6627-keto-8-aminopelargonic acid bindingBy similarity
    Regioni126 – 1272Pyridoxal phosphate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0161.
    HOGENOMiHOG000020209.
    KOiK00833.
    OMAiMATMAIC.
    OrthoDBiEOG7Q2NFK.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 2 hits.
    InterProiIPR005814. Aminotrans_3.
    IPR005815. BioA.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PANTHERiPTHR11986. PTHR11986. 1 hit.
    PfamiPF00202. Aminotran_3. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000521. Transaminase_4ab_Lys_Orn. 1 hit.
    SUPFAMiSSF53383. SSF53383. 1 hit.
    TIGRFAMsiTIGR00508. bioA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P50277-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQEISYTPD VAELLDFDKK HIWHPYTSLS SPLNVYPVKS AHGCKLVLDT    50
    DSPVDVEVID AMSSWWCVIH GYNNPELNEA LTKQMLKFSH VLLGGFTHKG 100
    AVNLVQKLLK VIDEPSLQYC FLADSGSVAV EVALKMALQS NMSGEATKNR 150
    TKFLTIKNGY HGDTFGAMSV CDPENSMHHI YNDRLSENIF AQAPSIVDGL 200
    PTSQNGFEDH WNAEEVTDLK KQFELHSDKI CAVILEPILQ GAGGLRPYHP 250
    QFLIEVQKLC NQYDVLFIMD EIATGFGRTG EIFAFKHCQK YQDQHGISPS 300
    DQIKVVPDIL CVGKGLTSGY MTMSAVVVND KVASRISSPN SPTGGCFMHG 350
    PTFMGNALAC SVAEKSMDIL LRGEWRKQVS AIENQIYREL YQYIKNPDNG 400
    LIGTVVKRVS VIGAVGIVEL YKKTDPEWFQ KKFISKGVHI RPFNCLCYIM 450
    PPYVITTEEL TKVNQVLIEV LHEWKSHINQ 480
    Length:480
    Mass (Da):53,709
    Last modified:October 1, 1996 - v1
    Checksum:i00A8BC42DCBED19A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti140 – 1401S → P in AAU09779. (PubMed:17322287)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti229 – 2291K → E in strain: ATCC 28383 and ATCC 204626. 1 Publication
    Natural varianti295 – 2951H → R in strain: ATCC 28383 and ATCC 204626. 1 Publication
    Natural varianti384 – 3841N → D in strain: ATCC 28383 and ATCC 204626. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U47112 Genomic DNA. Translation: AAA88905.1.
    U53467 Genomic DNA. Translation: AAB63970.1.
    AB200248 Genomic DNA. Translation: BAE00005.1.
    Z71673 Genomic DNA. Translation: CAA96340.1.
    AY723862 Genomic DNA. Translation: AAU09779.1.
    BK006947 Genomic DNA. Translation: DAA10599.1.
    PIRiS63390.
    RefSeqiNP_014456.1. NM_001183235.1.

    Genome annotation databases

    EnsemblFungiiYNR058W; YNR058W; YNR058W.
    GeneIDi855795.
    KEGGisce:YNR058W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U47112 Genomic DNA. Translation: AAA88905.1 .
    U53467 Genomic DNA. Translation: AAB63970.1 .
    AB200248 Genomic DNA. Translation: BAE00005.1 .
    Z71673 Genomic DNA. Translation: CAA96340.1 .
    AY723862 Genomic DNA. Translation: AAU09779.1 .
    BK006947 Genomic DNA. Translation: DAA10599.1 .
    PIRi S63390.
    RefSeqi NP_014456.1. NM_001183235.1.

    3D structure databases

    ProteinModelPortali P50277.
    SMRi P50277. Positions 15-473.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35884. 9 interactions.
    DIPi DIP-4822N.
    IntActi P50277. 1 interaction.
    MINTi MINT-562496.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNR058W ; YNR058W ; YNR058W .
    GeneIDi 855795.
    KEGGi sce:YNR058W.

    Organism-specific databases

    CYGDi YNR058w.
    SGDi S000005341. BIO3.

    Phylogenomic databases

    eggNOGi COG0161.
    HOGENOMi HOG000020209.
    KOi K00833.
    OMAi MATMAIC.
    OrthoDBi EOG7Q2NFK.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00160 .
    BioCyci YEAST:YNR058W-MONOMER.

    Miscellaneous databases

    NextBioi 980291.
    PROi P50277.

    Gene expression databases

    Genevestigatori P50277.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 2 hits.
    InterProi IPR005814. Aminotrans_3.
    IPR005815. BioA.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view ]
    PANTHERi PTHR11986. PTHR11986. 1 hit.
    Pfami PF00202. Aminotran_3. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000521. Transaminase_4ab_Lys_Orn. 1 hit.
    SUPFAMi SSF53383. SSF53383. 1 hit.
    TIGRFAMsi TIGR00508. bioA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of the biotin biosynthesis pathway in Saccharomyces cerevisiae and evidence for a cluster containing BIO5, a novel gene involved in vitamer uptake."
      Phalip V., Kuhn I., Lemoine Y., Jeltsch J.-M.
      Gene 232:43-51(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
      Strain: ATCC 28383 / FL100 / VTT C-80102.
    2. "Identification and characterization of a novel biotin biosynthesis gene in Saccharomyces cerevisiae."
      Wu H., Ito K., Shimoi H.
      Appl. Environ. Microbiol. 71:6845-6855(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS GLU-229; ARG-295 AND ASP-384.
      Strain: ATCC 204626 / S288c / A364A.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.

    Entry informationi

    Entry nameiBIOA_YEAST
    AccessioniPrimary (citable) accession number: P50277
    Secondary accession number(s): D6W1N3, E9P967, Q4R1J0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 130 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3