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Protein

High-affinity methionine permease

Gene

MUP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

High affinity permease for methionine.

GO - Molecular functioni

GO - Biological processi

  • cysteine transport Source: UniProtKB
  • methionine import Source: SGD
  • methionine transport Source: UniProtKB
  • sulfur amino acid transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30772-MONOMER.
ReactomeiR-SCE-352230. Amino acid transport across the plasma membrane.

Protein family/group databases

TCDBi2.A.3.8.4. the amino acid-polyamine-organocation (apc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
High-affinity methionine permease
Gene namesi
Name:MUP1
Ordered Locus Names:YGR055W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR055W.
SGDiS000003287. MUP1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6161CytoplasmicSequence analysisAdd
BLAST
Transmembranei62 – 8221HelicalSequence analysisAdd
BLAST
Topological domaini83 – 9210ExtracellularSequence analysis
Transmembranei93 – 11321HelicalSequence analysisAdd
BLAST
Topological domaini114 – 14027CytoplasmicSequence analysisAdd
BLAST
Transmembranei141 – 16121HelicalSequence analysisAdd
BLAST
Topological domaini162 – 18221ExtracellularSequence analysisAdd
BLAST
Transmembranei183 – 20321HelicalSequence analysisAdd
BLAST
Topological domaini204 – 2074CytoplasmicSequence analysis
Transmembranei208 – 22821HelicalSequence analysisAdd
BLAST
Topological domaini229 – 29365ExtracellularSequence analysisAdd
BLAST
Transmembranei294 – 31421HelicalSequence analysisAdd
BLAST
Topological domaini315 – 34026CytoplasmicSequence analysisAdd
BLAST
Transmembranei341 – 36121HelicalSequence analysisAdd
BLAST
Topological domaini362 – 41857ExtracellularSequence analysisAdd
BLAST
Transmembranei419 – 43921HelicalSequence analysisAdd
BLAST
Topological domaini440 – 45516CytoplasmicSequence analysisAdd
BLAST
Transmembranei456 – 47621HelicalSequence analysisAdd
BLAST
Topological domaini477 – 49014ExtracellularSequence analysisAdd
BLAST
Transmembranei491 – 51121HelicalSequence analysisAdd
BLAST
Topological domaini512 – 57463CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: SGD
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 574574High-affinity methionine permeasePRO_0000096648Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki28 – 28Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei552 – 5521PhosphothreonineCombined sources
Modified residuei573 – 5731PhosphoserineCombined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP50276.

PTM databases

iPTMnetiP50276.

Interactioni

Protein-protein interaction databases

BioGridi33300. 114 interactions.
DIPiDIP-7914N.
IntActiP50276. 57 interactions.
MINTiMINT-1357522.

Structurei

3D structure databases

ProteinModelPortaliP50276.
SMRiP50276. Positions 60-385.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

To yeast low affinity methionine permease (MUP3).Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00760000119037.
HOGENOMiHOG000187297.
InParanoidiP50276.
OMAiISITGWV.
OrthoDBiEOG7K9KC1.

Family and domain databases

InterProiIPR002293. AA/rel_permease1.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF13520. AA_permease_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006060. AA_transporter. 1 hit.

Sequencei

Sequence statusi: Complete.

P50276-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEGRTFLSQ LNVFNKENYQ FSSSTTKKEV SNSTVDADNG ASDFEAGQQF
60 70 80 90 100
ATELDQGEKQ LGILSCIGLI CNRMLGTGVF AVSSTIYTLC GSVGLALIMW
110 120 130 140 150
AVGAIIAISG LYVYMEFGTA IPKNGGEKNY LEAIFRKPKF FITCMYAAYI
160 170 180 190 200
FFLGWAAGNS INTAIMFLTA ADTEVTKWNQ RGIGVAVVFF AFLINSLNVK
210 220 230 240 250
IGLYLQNILG IFKIGIVLFI SITGWVALGG GLKDGYQSHN FRNAFEGTET
260 270 280 290 300
ATAYGIVNAL YSVIWSFVGY SNVNYALGEV KNPVRTLKIA GPTSMVFLAI
310 320 330 340 350
IYIFVNIAYF AVVPKDKLIS SKLILAADFF DIVFGGQAKR AAAALVGLSA
360 370 380 390 400
LGNVLSVIFS QGRIIQQLGR EGVLPFSNFF ASSKPFNSPM VGLFQHFIVC
410 420 430 440 450
TVTILAPPPG DAYLLVQNLI SYPMNIINFA ISAGLLWIYW QRRQGKIEWN
460 470 480 490 500
PPIKAGVFVT GFFTLSNLYL IIAPYVPPSN GESVYSSMPY WIHCVIAWGI
510 520 530 540 550
FFFGGVYYVV WAQLLPRWGH YKLVSKDVLG EDGFWRVKIA KVYDDTIGDV
560 570
DTQEDGVIET NIIEHYKSEQ EKSL
Length:574
Mass (Da):63,221
Last modified:October 1, 1996 - v1
Checksum:iF95D342814710837
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40316 Genomic DNA. Translation: AAB63529.1.
DQ115391 Genomic DNA. Translation: AAZ22468.1.
Z72840 Genomic DNA. Translation: CAA97055.1.
BK006941 Genomic DNA. Translation: DAA08151.1.
PIRiS61943.
RefSeqiNP_011569.1. NM_001181184.1.

Genome annotation databases

EnsemblFungiiYGR055W; YGR055W; YGR055W.
GeneIDi852946.
KEGGisce:YGR055W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40316 Genomic DNA. Translation: AAB63529.1.
DQ115391 Genomic DNA. Translation: AAZ22468.1.
Z72840 Genomic DNA. Translation: CAA97055.1.
BK006941 Genomic DNA. Translation: DAA08151.1.
PIRiS61943.
RefSeqiNP_011569.1. NM_001181184.1.

3D structure databases

ProteinModelPortaliP50276.
SMRiP50276. Positions 60-385.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33300. 114 interactions.
DIPiDIP-7914N.
IntActiP50276. 57 interactions.
MINTiMINT-1357522.

Protein family/group databases

TCDBi2.A.3.8.4. the amino acid-polyamine-organocation (apc) family.

PTM databases

iPTMnetiP50276.

Proteomic databases

MaxQBiP50276.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR055W; YGR055W; YGR055W.
GeneIDi852946.
KEGGisce:YGR055W.

Organism-specific databases

EuPathDBiFungiDB:YGR055W.
SGDiS000003287. MUP1.

Phylogenomic databases

GeneTreeiENSGT00760000119037.
HOGENOMiHOG000187297.
InParanoidiP50276.
OMAiISITGWV.
OrthoDBiEOG7K9KC1.

Enzyme and pathway databases

BioCyciYEAST:G3O-30772-MONOMER.
ReactomeiR-SCE-352230. Amino acid transport across the plasma membrane.

Miscellaneous databases

PROiP50276.

Family and domain databases

InterProiIPR002293. AA/rel_permease1.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF13520. AA_permease_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006060. AA_transporter. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The study of methionine uptake in Saccharomyces cerevisiae reveals a new family of amino acid permeases."
    Isnard A.D., Thomas D., Surdin-Kerjan Y.
    J. Mol. Biol. 262:473-484(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 26786 / X2180-1A.
  2. "Quantitative trait loci mapped to single-nucleotide resolution in yeast."
    Deutschbauer A.M., Davis R.W.
    Nat. Genet. 37:1333-1340(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: SK1.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-552, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-552 AND SER-573, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-28, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMUP1_YEAST
AccessioniPrimary (citable) accession number: P50276
Secondary accession number(s): D6VUJ0, Q45U31
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 6, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.