Reviewed, UniProtKB/Swiss-Prot P50275 (ASE1_YEAST)

Names and origin

Protein names

Recommended name:
    Anaphase spindle elongation protein

Gene names

Name:	ASE1
Ordered Locus Names:	YOR058C
ORF Names:	YOR29-09

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	885 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for anaphase spindle elongation.
Miscellaneous	Present with 556 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the MAP65/ASE1 family.

Ontologies

Keywords
Cellular component	Microtubule
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cytokinesis, completion of separation Inferred from mutant phenotype. Source: SGD microtubule bundle formation Inferred from direct assay. Source: SGD mitotic anaphase B Ref.1 Inferred from mutant phenotype. Source: SGD mitotic spindle elongation Ref.1 Inferred from mutant phenotype. Source: SGD mitotic spindle stabilization Inferred from mutant phenotype. Source: SGD spindle midzone assembly Inferred from mutant phenotype. Source: SGD spindle pole body separation Inferred from genetic interaction. Source: SGD
Cellular component	nuclear microtubule Ref.1 Inferred from direct assay. Source: SGD spindle midzone Ref.1 Inferred from direct assay. Source: SGD
Molecular function	microtubule binding Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 885	885	Anaphase spindle elongation protein		PRO_0000064694

Sequences

	Sequence		Length	Mass (Da)
	P50275-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 6EDE11E887E89207	FASTA	885	101,624
	10 20 30 40 50 60 METATSSPLP IKSRRNSENS GSTTVIPHMN PSLATPLTVS TMVNQSNSKE FMKLTPVRIR 70 80 90 100 110 120 DFGSPLKNVS TNYHFLDSEN GKGNTMDNMY RENFILISKD LEKLLENLNV IYQNIGYSNT 130 140 150 160 170 180 EIITKEKIIF TTISNSIKQF FEQADEELKR LSAENGIEQD ILNNILERIN DPSGIKTIPD 190 200 210 220 230 240 LYIRNAILLQ ESKTVPQSPK KPLSLLSKKA ALDTAKKFVL GSFLPRLRDY LKSLITLKHL 250 260 270 280 290 300 IQSVKENLPG LTEADNEAIA EFPELSTLTA YLLQIENGKG DIGLSMKFII DNRKDILKGS 310 320 330 340 350 360 AFKTINEESV KHMNEVIKIY EEEYERRFKS VLTKKVSISS ICEQLGTPLA TLIGEDFEQD 370 380 390 400 410 420 LRSYGEEENS TSEIPNFHPV DRERMSKIDI TLEKLQAIHK ERADKKRLLM EQCQKLWTRL 430 440 450 460 470 480 KISQEYIKTF MRNNSSLSTE SLGRISKEVM RLEAMKKKLI KKLISDSWDK IQELWRTLQY 490 500 510 520 530 540 SEESRSKFII VFEELRNSAT TLQEDELLLE TCENELKRLE EKLTLYKPIL KLISDFESLQ 550 560 570 580 590 600 EDQEFLERSS KDSSRLLSRN SHKILLTEEK MRKRITRHFP RVINDLRIKL EEADGLFDQP 610 620 630 640 650 660 FLFKGKPLSE AIDIQQQEIE AKYPRCRVRM QRSKKGKCGA NKENKVIKNT FKATESSIRV 670 680 690 700 710 720 PIGLNLNDAN ITYKTPSKKT IQGLTKNDLS QENSLARHMQ GTTKLSSPNR RATRLLAPTV 730 740 750 760 770 780 ISRNSKGNIE RPTLNRNRSS DLSSSPRINH THGEHAVKPR QLFPIPLNKV DTKGSHIPQL 790 800 810 820 830 840 TKEKALELLK RSTGTTGKEN VRSPERKSSL EDYAQKLSSP YKEPEHSIYK LSMSPEGKFQ 850 860 870 880 LNIQQKDIES GFDDTSMMED ENDKDFITWK NEQVSKLNGF SFTDI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two microtubule-associated proteins required for anaphase spindle movement in Saccharomyces cerevisiae." Pellman D., Bagget M., Tu Y.H., Fink G.R. J. Cell Biol. 130:1373-1385(1995) [PubMed: 7559759] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The sequence of a 54.7 kb fragment of yeast chromosome XV reveals the presence of two tRNAs and 24 new open reading frames." Valens M., Bohn C., Daignan-Fornier B., Dang V.-D., Bolotin-Fukuhara M. Yeast 13:379-390(1997) [PubMed: 9133743] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U20235 Genomic DNA. Translation: AAA75026.1. Z74966 Genomic DNA. Translation: CAA99251.1. Z70678 Genomic DNA. Translation: CAA94543.1.
PIR	S59660.
RefSeq	NP_116582.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:7343N.
IntAct	P50275. 1 interaction.
STRING	P50275.
Proteomic databases
PRIDE	P50275.
Genome annotation databases
Ensembl	YOR058C; YOR058C; YOR058C; Saccharomyces cerevisiae. [Genome view]
GeneID	854223.
GenomeReviews	Gene locus YOR058C in contig Y13140_GR.
KEGG	sce:YOR058C.
NMPDR	fig|4932.3.peg.5801.
Organism-specific databases
CYGD	YOR058c.
SGD	S000005584. ASE1.
Phylogenomic databases
HOGENOM	P50275.
OMA	RNSHKIL.
Gene expression databases
ArrayExpress	P50275.
Genevestigator	P50275.
GermOnline	YOR058C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007145. MAP65_ASE1. [Graphical view]
PANTHER	PTHR19321. MAP65_ASE1. 1 hit.
Pfam	PF03999. MAP65_ASE1. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	976092.

Entry information

Entry name

ASE1_YEAST

Accession

Primary (citable) accession number: P50275

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 3, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)