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P50264 (FMS1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Polyamine oxidase FMS1
EC=1.5.3.17
Alternative name(s):
Fenpropimorph resistance multicopy suppressor 1
Gene names
Name:FMS1
Ordered Locus Names:YMR020W
ORF Names:YM9711.09
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length508 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the production of beta-alanine, a precursor of pantothenic acid. Multicopy suppressor of fenpropimorph resistance. Ref.4 Ref.6

Catalytic activity

Spermine + O2 + H2O = spermidine + 3-aminopropanal + H2O2.

Spermidine + O2 + H2O = putrescine + 3-aminopropanal + H2O2.

N(1)-acetylspermine + O2 + H2O = spermidine + 3-acetamidopropanal + H2O2.

N(1)-acetylspermidine + O2 + H2O = putrescine + 3-acetamidopropanal + H2O2.

N(8)-acetylspermidine + O2 + H2O = 4-acetamidobutanal + trimethylenediamine + H2O2.

Cofactor

Binds 1 FAD per subunit. Ref.4

Miscellaneous

Present with 6960 molecules/cell in log phase SD medium. Ref.5

Sequence similarities

Belongs to the flavin monoamine oxidase family.

Ontologies

Keywords
   LigandFAD
Flavoprotein
   Molecular functionOxidoreductase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological processpantothenate biosynthetic process

Inferred from mutant phenotype. Source: SGD

polyamine catabolic process

Inferred from genetic interaction. Source: SGD

   Cellular componentcytoplasm

Inferred from direct assay. Source: SGD

   Molecular functionpolyamine oxidase activity

Inferred from direct assay. Source: SGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 508508Polyamine oxidase FMS1
PRO_0000087317

Secondary structure

1 ..................................................................................... 508
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P50264 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 8E135295301EB3CF

FASTA50857,806
        10         20         30         40         50         60 
MNTVSPAKKK VIIIGAGIAG LKAASTLHQN GIQDCLVLEA RDRVGGRLQT VTGYQGRKYD 

        70         80         90        100        110        120 
IGASWHHDTL TNPLFLEEAQ LSLNDGRTRF VFDDDNFIYI DEERGRVDHD KELLLEIVDN 

       130        140        150        160        170        180 
EMSKFAELEF HQHLGVSDCS FFQLVMKYLL QRRQFLTNDQ IRYLPQLCRY LELWHGLDWK 

       190        200        210        220        230        240 
LLSAKDTYFG HQGRNAFALN YDSVVQRIAQ SFPQNWLKLS CEVKSITREP SKNVTVNCED 

       250        260        270        280        290        300 
GTVYNADYVI ITVPQSVLNL SVQPEKNLRG RIEFQPPLKP VIQDAFDKIH FGALGKVIFE 

       310        320        330        340        350        360 
FEECCWSNES SKIVTLANST NEFVEIVRNA ENLDELDSML EREDSQKHTS VTCWSQPLFF 

       370        380        390        400        410        420 
VNLSKSTGVA SFMMLMQAPL TNHIESIRED KERLFSFFQP VLNKIMKCLD SEDVIDGMRP 

       430        440        450        460        470        480 
IENIANANKP VLRNIIVSNW TRDPYSRGAY SACFPGDDPV DMVVAMSNGQ DSRIRFAGEH 

       490        500 
TIMDGAGCAY GAWESGRREA TRISDLLK

« Hide

References

« Hide 'large scale' references
[1]"Characterization of the Saccharomyces cerevisiae FMS1 gene related to Candida albicans corticosteroid-binding protein 1."
Joets J., Pousset D., Marcireau C., Karst F.
Curr. Genet. 30:115-120(1996) [PubMed: 8660467] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed: 9169872] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Yeast Fms1 is a FAD-utilizing polyamine oxidase."
Landry J., Sternglanz R.
Biochem. Biophys. Res. Commun. 303:771-776(2003) [PubMed: 12670477] [Abstract]
Cited for: FUNCTION, COFACTOR.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Spermidine but not spermine is essential for hypusine biosynthesis and growth in Saccharomyces cerevisiae: spermine is converted to spermidine in vivo by the FMS1-amine oxidase."
Chattopadhyay M.K., Tabor C.W., Tabor H.
Proc. Natl. Acad. Sci. U.S.A. 100:13869-13874(2003) [PubMed: 14617780] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X81848 Genomic DNA. Translation: CAA57442.1.
Z49211 Genomic DNA. Translation: CAA89122.1.
BK006946 Genomic DNA. Translation: DAA09918.1.
PIRS54021.
RefSeqNP_013733.1. NM_001182516.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1RSGX-ray1.90A/B1-508[»]
1XPQX-ray2.60A/B/C/D1-508[»]
1YY5X-ray2.30A/B1-508[»]
1Z6LX-ray2.50A/B1-508[»]
3BI2X-ray2.30A/B1-508[»]
3BI4X-ray2.20A/B1-508[»]
3BI5X-ray2.50A/B1-508[»]
3BNMX-ray2.20A/B1-508[»]
3BNUX-ray2.20A/B1-508[»]
3CN8X-ray2.40A/B1-508[»]
3CNDX-ray2.50A/B1-508[»]
3CNPX-ray2.50A/B1-508[»]
3CNSX-ray2.40A/B1-508[»]
3CNTX-ray2.70A/B1-508[»]
ProteinModelPortalP50264.
SMRP50264. Positions 8-508.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-3959N.
MINTMINT-492079.
STRINGP50264.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR020W; YMR020W; YMR020W.
GeneID855034.
KEGGsce:YMR020W.
NMPDRfig|4932.3.peg.4779.

Organism-specific databases

CYGDYMR020w.
SGDS000004622. FMS1.

Phylogenomic databases

eggNOGfuNOG05690.
GeneTreeEFGT00050000002560.
HOGENOMHBG398221.
OMAAGEHTIM.
OrthoDBEOG4M0J9J.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-13663.

Gene expression databases

ArrayExpressP50264.
GenevestigatorP50264.
GermOnlineYMR020W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR002937. Amino_oxidase.
[Graphical view]
KOK13367.
PfamPF01593. Amino_oxidase. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio978242.

Entry information

Entry nameFMS1_YEAST
AccessionPrimary (citable) accession number: P50264
Secondary accession number(s): D6VZJ4
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: December 14, 2011
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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