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P50233

- DHI2_RAT

UniProt

P50233 - DHI2_RAT

Protein

Corticosteroid 11-beta-dehydrogenase isozyme 2

Gene

Hsd11b2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of cortisol to the inactive metabolite cortisone. Modulates intracellular glucocorticoid levels, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids.

    Catalytic activityi

    An 11-beta-hydroxysteroid + NAD+ = an 11-oxosteroid + NADH.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei219 – 2191SubstrateBy similarity
    Active sitei232 – 2321Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi82 – 11130NADBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. 11-beta-hydroxysteroid dehydrogenase [NAD(P)] activity Source: RGD
    2. NAD binding Source: RGD
    3. oxidoreductase activity Source: RGD
    4. steroid binding Source: RGD

    GO - Biological processi

    1. female pregnancy Source: RGD
    2. glucocorticoid metabolic process Source: RGD
    3. metabolic process Source: RGD
    4. regulation of blood volume by renal aldosterone Source: RGD
    5. response to drug Source: RGD
    6. response to food Source: RGD
    7. response to glucocorticoid Source: RGD
    8. response to hypoxia Source: RGD
    9. response to insulin Source: RGD
    10. response to steroid hormone Source: RGD

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NAD

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Corticosteroid 11-beta-dehydrogenase isozyme 2 (EC:1.1.1.-)
    Alternative name(s):
    11-beta-hydroxysteroid dehydrogenase type 2
    Short name:
    11-DH2
    Short name:
    11-beta-HSD2
    NAD-dependent 11-beta-hydroxysteroid dehydrogenase
    Gene namesi
    Name:Hsd11b2
    Synonyms:Hsd11k
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 19

    Organism-specific databases

    RGDi2835. Hsd11b2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: RGD
    2. endoplasmic reticulum Source: UniProtKB-SubCell
    3. intracellular membrane-bounded organelle Source: RGD

    Keywords - Cellular componenti

    Endoplasmic reticulum, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 400400Corticosteroid 11-beta-dehydrogenase isozyme 2PRO_0000054630Add
    BLAST

    Proteomic databases

    PaxDbiP50233.
    PRIDEiP50233.

    Expressioni

    Tissue specificityi

    Highly expressed in kidney, adrenal gland and distal colon. Detected at much lower levels in lung.

    Gene expression databases

    GenevestigatoriP50233.

    Interactioni

    Subunit structurei

    Interacts with ligand-free cytoplasmic NR3C2.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000023130.

    Structurei

    3D structure databases

    ProteinModelPortaliP50233.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1028.
    GeneTreeiENSGT00650000093222.
    HOVERGENiHBG005482.
    InParanoidiP50233.
    KOiK00071.
    OMAiCFKTESV.
    OrthoDBiEOG7FXZZX.
    PhylomeDBiP50233.
    TreeFamiTF325617.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    [Graphical view]
    PfamiPF00106. adh_short. 1 hit.
    [Graphical view]
    PRINTSiPR00081. GDHRDH.
    PROSITEiPS00061. ADH_SHORT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P50233-1 [UniParc]FASTAAdd to Basket

    « Hide

    MERWPWPSGG AWLLVAARAL LQLLRSDLRL GRPLLAALAL LAALDWLCQR    50
    LLPPPAALVV LAGAGWIALS RLARPPRLPV ATRAVLITGC DTGFGKETAK 100
    KLDAMGFTVL ATVLDLNGPG ALELRARCSP RLKLLQMDLT KPEDISRVLE 150
    ITKAHTASTG LWGLVNNAGL NMVVADVELS PVVTFRECME VNFFGALELT 200
    KGLLPLLRHS RGRIVTVGSP AGDMPYPCLA AYGTSKAAIA LLMDTFSCEL 250
    LPWGIKVSII QPGCFKTEAV TNVNLWEKRK QLLLANLPRE LLQAYGEDYI 300
    EHLHGQFLNS LRMALPDLSP VVDAIIDALL AAQPRSRYYT GRGLGLMYFI 350
    HHYLPGGLRR RFLQNFFISH LLPRALRPGQ PGPVHDTTQD PNPSPTVSAL 400
    Length:400
    Mass (Da):43,726
    Last modified:October 1, 1996 - v1
    Checksum:iA1BAAA328E2F189D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U22424 mRNA. Translation: AAA87007.1.
    BC087023 mRNA. Translation: AAH87023.1.
    RefSeqiNP_058777.1. NM_017081.2.
    UniGeneiRn.10186.

    Genome annotation databases

    EnsembliENSRNOT00000023130; ENSRNOP00000023130; ENSRNOG00000017084.
    GeneIDi25117.
    KEGGirno:25117.
    UCSCiRGD:2835. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U22424 mRNA. Translation: AAA87007.1 .
    BC087023 mRNA. Translation: AAH87023.1 .
    RefSeqi NP_058777.1. NM_017081.2.
    UniGenei Rn.10186.

    3D structure databases

    ProteinModelPortali P50233.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000023130.

    Chemistry

    BindingDBi P50233.
    ChEMBLi CHEMBL2908.

    Proteomic databases

    PaxDbi P50233.
    PRIDEi P50233.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000023130 ; ENSRNOP00000023130 ; ENSRNOG00000017084 .
    GeneIDi 25117.
    KEGGi rno:25117.
    UCSCi RGD:2835. rat.

    Organism-specific databases

    CTDi 3291.
    RGDi 2835. Hsd11b2.

    Phylogenomic databases

    eggNOGi COG1028.
    GeneTreei ENSGT00650000093222.
    HOVERGENi HBG005482.
    InParanoidi P50233.
    KOi K00071.
    OMAi CFKTESV.
    OrthoDBi EOG7FXZZX.
    PhylomeDBi P50233.
    TreeFami TF325617.

    Miscellaneous databases

    NextBioi 605485.
    PROi P50233.

    Gene expression databases

    Genevestigatori P50233.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    [Graphical view ]
    Pfami PF00106. adh_short. 1 hit.
    [Graphical view ]
    PRINTSi PR00081. GDHRDH.
    PROSITEi PS00061. ADH_SHORT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, expression, and tissue distribution of the rat nicotinamide adenine dinucleotide-dependent 11 beta-hydroxysteroid dehydrogenase."
      Zhou M.-Y., Gomez-Sanchez E.P., Cox D.L., Cosby D., Gomez-Sanchez C.E.
      Endocrinology 136:3729-3734(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Kidney.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.
    3. Lubec G., Chen W.-Q.
      Submitted (JAN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 19-25, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Hippocampus.

    Entry informationi

    Entry nameiDHI2_RAT
    AccessioniPrimary (citable) accession number: P50233
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 98 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3