P50233 (DHI2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Corticosteroid 11-beta-dehydrogenase isozyme 2
EC=1.1.1.-

Alternative name(s):
11-beta-hydroxysteroid dehydrogenase type 2
Short name=11-DH2
Short name=11-beta-HSD2
NAD-dependent 11-beta-hydroxysteroid dehydrogenase

Gene names

Name:	Hsd11b2
Synonyms:	Hsd11k

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	400 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the conversion of cortisol to the inactive metabolite cortisone. Modulates intracellular glucocorticoid levels, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids.
Catalytic activity	An 11-beta-hydroxysteroid + NAD+ = an 11-oxosteroid + NADH.
Subunit structure	Interacts with ligand-free cytoplasmic NR3C2 By similarity.
Subcellular location	Microsome. Endoplasmic reticulum Potential.
Tissue specificity	Highly expressed in kidney, adrenal gland and distal colon. Detected at much lower levels in lung.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Microsome
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	female pregnancy Inferred from expression pattern PubMed 19050325. Source: RGD glucocorticoid metabolic process Inferred from direct assay PubMed 16763064. Source: RGD regulation of blood volume by renal aldosterone Inferred from mutant phenotype PubMed 15718388. Source: RGD response to drug Inferred from expression pattern PubMed 10792625. Source: RGD response to food Inferred from expression pattern PubMed 18548384. Source: RGD response to glucocorticoid stimulus Inferred from expression pattern PubMed 19490994. Source: RGD response to hypoxia Inferred from expression pattern PubMed 19470702. Source: RGD response to insulin stimulus Inferred from expression pattern PubMed 9495277. Source: RGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 15761036. Source: RGD endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell intracellular membrane-bounded organelle Inferred from direct assay PubMed 15761036. Source: RGD
Molecular_function	11-beta-hydroxysteroid dehydrogenase [NAD(P)] activity Inferred from direct assay PubMed 11755176. Source: RGD NAD binding Inferred from direct assay PubMed 15761036. Source: RGD steroid binding Inferred from physical interaction PubMed 15761036. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 400	400	Corticosteroid 11-beta-dehydrogenase isozyme 2		PRO_0000054630
Regions
	Nucleotide binding	82 – 111	30	NAD By similarity
Sites
	Active site	232	1	Proton acceptor By similarity
	Binding site	219	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P50233 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: A1BAAA328E2F189D	FASTA	400	43,726
	10 20 30 40 50 60 MERWPWPSGG AWLLVAARAL LQLLRSDLRL GRPLLAALAL LAALDWLCQR LLPPPAALVV 70 80 90 100 110 120 LAGAGWIALS RLARPPRLPV ATRAVLITGC DTGFGKETAK KLDAMGFTVL ATVLDLNGPG 130 140 150 160 170 180 ALELRARCSP RLKLLQMDLT KPEDISRVLE ITKAHTASTG LWGLVNNAGL NMVVADVELS 190 200 210 220 230 240 PVVTFRECME VNFFGALELT KGLLPLLRHS RGRIVTVGSP AGDMPYPCLA AYGTSKAAIA 250 260 270 280 290 300 LLMDTFSCEL LPWGIKVSII QPGCFKTEAV TNVNLWEKRK QLLLANLPRE LLQAYGEDYI 310 320 330 340 350 360 EHLHGQFLNS LRMALPDLSP VVDAIIDALL AAQPRSRYYT GRGLGLMYFI HHYLPGGLRR 370 380 390 400 RFLQNFFISH LLPRALRPGQ PGPVHDTTQD PNPSPTVSAL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, expression, and tissue distribution of the rat nicotinamide adenine dinucleotide-dependent 11 beta-hydroxysteroid dehydrogenase." Zhou M.-Y., Gomez-Sanchez E.P., Cox D.L., Cosby D., Gomez-Sanchez C.E. Endocrinology 136:3729-3734(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Kidney.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[3]	Lubec G., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 19-25, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Hippocampus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U22424 mRNA. Translation: AAA87007.1. BC087023 mRNA. Translation: AAH87023.1.
IPI	IPI00196451.
RefSeq	NP_058777.1. NM_017081.2.
UniGene	Rn.10186.
3D structure databases
ProteinModelPortal	P50233.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000023130.
Proteomic databases
PaxDb	P50233.
PRIDE	P50233.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000023130; ENSRNOP00000023130; ENSRNOG00000017084.
GeneID	25117.
KEGG	rno:25117.
UCSC	RGD:2835. rat.
Organism-specific databases
CTD	3291.
RGD	2835. Hsd11b2.
Phylogenomic databases
eggNOG	COG1028.
GeneTree	ENSGT00650000093222.
HOVERGEN	HBG005482.
InParanoid	P50233.
KO	K00071.
OMA	CFKTESV.
OrthoDB	EOG49CQ89.
Gene expression databases
ArrayExpress	P50233.
Genevestigator	P50233.
GermOnline	ENSRNOG00000017084. Rattus norvegicus.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR020904. Sc_DH/Rdtase_CS. [Graphical view]
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P50233.
ChEMBL	CHEMBL2908.
NextBio	605485.

Entry information

Entry name

DHI2_RAT

Accession

Primary (citable) accession number: P50233

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program