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P50233 (DHI2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Corticosteroid 11-beta-dehydrogenase isozyme 2

EC=1.1.1.-
Alternative name(s):
11-beta-hydroxysteroid dehydrogenase type 2
Short name=11-DH2
Short name=11-beta-HSD2
NAD-dependent 11-beta-hydroxysteroid dehydrogenase
Gene names
Name:Hsd11b2
Synonyms:Hsd11k
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length400 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the conversion of cortisol to the inactive metabolite cortisone. Modulates intracellular glucocorticoid levels, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids.

Catalytic activity

An 11-beta-hydroxysteroid + NAD+ = an 11-oxosteroid + NADH.

Subunit structure

Interacts with ligand-free cytoplasmic NR3C2 By similarity.

Subcellular location

Microsome. Endoplasmic reticulum Potential.

Tissue specificity

Highly expressed in kidney, adrenal gland and distal colon. Detected at much lower levels in lung.

Sequence similarities

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
   Cellular componentEndoplasmic reticulum
Microsome
   LigandNAD
   Molecular functionOxidoreductase
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processfemale pregnancy

Inferred from expression pattern PubMed 19050325. Source: RGD

glucocorticoid metabolic process

Inferred from direct assay PubMed 16763064. Source: RGD

metabolic process

Inferred from direct assay PubMed 11751610. Source: RGD

regulation of blood volume by renal aldosterone

Inferred from mutant phenotype PubMed 15718388. Source: RGD

response to drug

Inferred from expression pattern PubMed 10792625. Source: RGD

response to food

Inferred from expression pattern PubMed 18548384. Source: RGD

response to glucocorticoid

Inferred from expression pattern PubMed 19490994. Source: RGD

response to hypoxia

Inferred from expression pattern PubMed 19470702. Source: RGD

response to insulin

Inferred from expression pattern PubMed 9495277. Source: RGD

response to steroid hormone

Inferred from expression pattern PubMed 18032797. Source: RGD

   Cellular_componentcytoplasm

Inferred from direct assay PubMed 15761036. Source: RGD

endoplasmic reticulum

Inferred from electronic annotation. Source: UniProtKB-SubCell

intracellular membrane-bounded organelle

Inferred from direct assay PubMed 15761036. Source: RGD

   Molecular_function11-beta-hydroxysteroid dehydrogenase [NAD(P)] activity

Inferred from direct assay PubMed 11755176. Source: RGD

NAD binding

Inferred from direct assay PubMed 15761036. Source: RGD

oxidoreductase activity

Inferred from direct assay PubMed 11751610. Source: RGD

steroid binding

Inferred from physical interaction PubMed 15761036. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 400400Corticosteroid 11-beta-dehydrogenase isozyme 2
PRO_0000054630

Regions

Nucleotide binding82 – 11130NAD By similarity

Sites

Active site2321Proton acceptor By similarity
Binding site2191Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
P50233 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: A1BAAA328E2F189D

FASTA40043,726
        10         20         30         40         50         60 
MERWPWPSGG AWLLVAARAL LQLLRSDLRL GRPLLAALAL LAALDWLCQR LLPPPAALVV 

        70         80         90        100        110        120 
LAGAGWIALS RLARPPRLPV ATRAVLITGC DTGFGKETAK KLDAMGFTVL ATVLDLNGPG 

       130        140        150        160        170        180 
ALELRARCSP RLKLLQMDLT KPEDISRVLE ITKAHTASTG LWGLVNNAGL NMVVADVELS 

       190        200        210        220        230        240 
PVVTFRECME VNFFGALELT KGLLPLLRHS RGRIVTVGSP AGDMPYPCLA AYGTSKAAIA 

       250        260        270        280        290        300 
LLMDTFSCEL LPWGIKVSII QPGCFKTEAV TNVNLWEKRK QLLLANLPRE LLQAYGEDYI 

       310        320        330        340        350        360 
EHLHGQFLNS LRMALPDLSP VVDAIIDALL AAQPRSRYYT GRGLGLMYFI HHYLPGGLRR 

       370        380        390        400 
RFLQNFFISH LLPRALRPGQ PGPVHDTTQD PNPSPTVSAL 

« Hide

References

« Hide 'large scale' references
[1]"Cloning, expression, and tissue distribution of the rat nicotinamide adenine dinucleotide-dependent 11 beta-hydroxysteroid dehydrogenase."
Zhou M.-Y., Gomez-Sanchez E.P., Cox D.L., Cosby D., Gomez-Sanchez C.E.
Endocrinology 136:3729-3734(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Kidney.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.
[3]Lubec G., Chen W.-Q.
Submitted (JAN-2009) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 19-25, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: Sprague-Dawley.
Tissue: Hippocampus.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U22424 mRNA. Translation: AAA87007.1.
BC087023 mRNA. Translation: AAH87023.1.
RefSeqNP_058777.1. NM_017081.2.
UniGeneRn.10186.

3D structure databases

ProteinModelPortalP50233.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000023130.

Chemistry

BindingDBP50233.
ChEMBLCHEMBL2908.

Proteomic databases

PaxDbP50233.
PRIDEP50233.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000023130; ENSRNOP00000023130; ENSRNOG00000017084.
GeneID25117.
KEGGrno:25117.
UCSCRGD:2835. rat.

Organism-specific databases

CTD3291.
RGD2835. Hsd11b2.

Phylogenomic databases

eggNOGCOG1028.
GeneTreeENSGT00650000093222.
HOVERGENHBG005482.
InParanoidP50233.
KOK00071.
OMACFKTESV.
OrthoDBEOG7FXZZX.
PhylomeDBP50233.
TreeFamTF325617.

Gene expression databases

GenevestigatorP50233.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSPR00081. GDHRDH.
PROSITEPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio605485.
PROP50233.

Entry information

Entry nameDHI2_RAT
AccessionPrimary (citable) accession number: P50233
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 14, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families