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P50233

- DHI2_RAT

UniProt

P50233 - DHI2_RAT

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Protein

Corticosteroid 11-beta-dehydrogenase isozyme 2

Gene

Hsd11b2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of cortisol to the inactive metabolite cortisone. Modulates intracellular glucocorticoid levels, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids.

Catalytic activityi

An 11-beta-hydroxysteroid + NAD+ = an 11-oxosteroid + NADH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei219 – 2191SubstrateBy similarity
Active sitei232 – 2321Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi82 – 11130NADBy similarityAdd
BLAST

GO - Molecular functioni

  1. 11-beta-hydroxysteroid dehydrogenase [NAD(P)] activity Source: RGD
  2. NAD binding Source: RGD
  3. oxidoreductase activity Source: RGD
  4. steroid binding Source: RGD

GO - Biological processi

  1. female pregnancy Source: RGD
  2. glucocorticoid metabolic process Source: RGD
  3. metabolic process Source: RGD
  4. regulation of blood volume by renal aldosterone Source: RGD
  5. response to drug Source: RGD
  6. response to food Source: RGD
  7. response to glucocorticoid Source: RGD
  8. response to hypoxia Source: RGD
  9. response to insulin Source: RGD
  10. response to steroid hormone Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Corticosteroid 11-beta-dehydrogenase isozyme 2 (EC:1.1.1.-)
Alternative name(s):
11-beta-hydroxysteroid dehydrogenase type 2
Short name:
11-DH2
Short name:
11-beta-HSD2
NAD-dependent 11-beta-hydroxysteroid dehydrogenase
Gene namesi
Name:Hsd11b2
Synonyms:Hsd11k
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 19

Organism-specific databases

RGDi2835. Hsd11b2.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: RGD
  2. endoplasmic reticulum Source: UniProtKB-KW
  3. intracellular membrane-bounded organelle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 400400Corticosteroid 11-beta-dehydrogenase isozyme 2PRO_0000054630Add
BLAST

Proteomic databases

PaxDbiP50233.
PRIDEiP50233.

Expressioni

Tissue specificityi

Highly expressed in kidney, adrenal gland and distal colon. Detected at much lower levels in lung.

Gene expression databases

ExpressionAtlasiP50233. baseline and differential.
GenevestigatoriP50233.

Interactioni

Subunit structurei

Interacts with ligand-free cytoplasmic NR3C2.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023130.

Structurei

3D structure databases

ProteinModelPortaliP50233.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1028.
GeneTreeiENSGT00770000120485.
HOVERGENiHBG005482.
InParanoidiP50233.
KOiK00071.
OMAiCFKTESV.
OrthoDBiEOG7FXZZX.
PhylomeDBiP50233.
TreeFamiTF325617.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50233-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MERWPWPSGG AWLLVAARAL LQLLRSDLRL GRPLLAALAL LAALDWLCQR
60 70 80 90 100
LLPPPAALVV LAGAGWIALS RLARPPRLPV ATRAVLITGC DTGFGKETAK
110 120 130 140 150
KLDAMGFTVL ATVLDLNGPG ALELRARCSP RLKLLQMDLT KPEDISRVLE
160 170 180 190 200
ITKAHTASTG LWGLVNNAGL NMVVADVELS PVVTFRECME VNFFGALELT
210 220 230 240 250
KGLLPLLRHS RGRIVTVGSP AGDMPYPCLA AYGTSKAAIA LLMDTFSCEL
260 270 280 290 300
LPWGIKVSII QPGCFKTEAV TNVNLWEKRK QLLLANLPRE LLQAYGEDYI
310 320 330 340 350
EHLHGQFLNS LRMALPDLSP VVDAIIDALL AAQPRSRYYT GRGLGLMYFI
360 370 380 390 400
HHYLPGGLRR RFLQNFFISH LLPRALRPGQ PGPVHDTTQD PNPSPTVSAL
Length:400
Mass (Da):43,726
Last modified:October 1, 1996 - v1
Checksum:iA1BAAA328E2F189D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22424 mRNA. Translation: AAA87007.1.
BC087023 mRNA. Translation: AAH87023.1.
RefSeqiNP_058777.1. NM_017081.2.
UniGeneiRn.10186.

Genome annotation databases

EnsembliENSRNOT00000023130; ENSRNOP00000023130; ENSRNOG00000017084.
GeneIDi25117.
KEGGirno:25117.
UCSCiRGD:2835. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22424 mRNA. Translation: AAA87007.1 .
BC087023 mRNA. Translation: AAH87023.1 .
RefSeqi NP_058777.1. NM_017081.2.
UniGenei Rn.10186.

3D structure databases

ProteinModelPortali P50233.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000023130.

Chemistry

BindingDBi P50233.
ChEMBLi CHEMBL2908.

Proteomic databases

PaxDbi P50233.
PRIDEi P50233.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000023130 ; ENSRNOP00000023130 ; ENSRNOG00000017084 .
GeneIDi 25117.
KEGGi rno:25117.
UCSCi RGD:2835. rat.

Organism-specific databases

CTDi 3291.
RGDi 2835. Hsd11b2.

Phylogenomic databases

eggNOGi COG1028.
GeneTreei ENSGT00770000120485.
HOVERGENi HBG005482.
InParanoidi P50233.
KOi K00071.
OMAi CFKTESV.
OrthoDBi EOG7FXZZX.
PhylomeDBi P50233.
TreeFami TF325617.

Miscellaneous databases

NextBioi 605485.
PROi P50233.

Gene expression databases

ExpressionAtlasi P50233. baseline and differential.
Genevestigatori P50233.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view ]
Pfami PF00106. adh_short. 1 hit.
[Graphical view ]
PRINTSi PR00081. GDHRDH.
PROSITEi PS00061. ADH_SHORT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, expression, and tissue distribution of the rat nicotinamide adenine dinucleotide-dependent 11 beta-hydroxysteroid dehydrogenase."
    Zhou M.-Y., Gomez-Sanchez E.P., Cox D.L., Cosby D., Gomez-Sanchez C.E.
    Endocrinology 136:3729-3734(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Kidney.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  3. Lubec G., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 19-25, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Hippocampus.

Entry informationi

Entry nameiDHI2_RAT
AccessioniPrimary (citable) accession number: P50233
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 26, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3