Synaptotagmin-4 - Rattus norvegicus (Rat)

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P50232 (SYT4_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified September 21, 2011. Version 94. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Synaptotagmin-4

Alternative name(s):
Synaptotagmin IV
Short name=SytIV

Gene names

Name:

Syt4

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	425 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in Ca²⁺-dependent exocytosis of secretory vesicles through Ca²⁺ and phospholipid binding to the C2 domain or may serve as Ca²⁺ sensors in the process of vesicular trafficking and exocytosis.
Cofactor	Binds 3 calcium ions per subunit. The ions are bound to the C2 domains By similarity.
Subcellular location	Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane; Single-pass membrane protein.
Sequence similarities	Belongs to the synaptotagmin family. Contains 2 C2 domains.

Ontologies

Keywords
Cellular component	Cell junction Cytoplasmic vesicle Membrane Synapse
Domain	Repeat Transmembrane Transmembrane helix
Ligand	Calcium Metal-binding
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	calcium ion-dependent exocytosis Non-traceable author statement Ref.2. Source: RGD neurotransmitter secretion Non-traceable author statement Ref.2. Source: RGD
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW synaptic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	clathrin binding Inferred from direct assay. Source: BHF-UCL transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 425

425

Synaptotagmin-4

PRO_0000183950

Regions

Topological domain

1 – 16

Vesicular Potential

Transmembrane

17 – 37

Helical; Potential

Topological domain

38 – 425

388

Cytoplasmic Potential

Domain

155 – 258

104

C2 1

Domain

289 – 392

104

C2 2

Region

147 – 297

151

Phospholipid binding Probable

Sites

Metal binding

184

Calcium 1 By similarity

Metal binding

184

Calcium 2 By similarity

Metal binding

191

Calcium 1 By similarity

Metal binding

245

Calcium 1; via carbonyl oxygen By similarity

Metal binding

246

Calcium 1 By similarity

Metal binding

246

Calcium 2 By similarity

Metal binding

246

Calcium 3 By similarity

Metal binding

249

Calcium 3 By similarity

Metal binding

252

Calcium 2 By similarity

Metal binding

252

Calcium 3 By similarity

Secondary structure

425

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P50232 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 6AC88E00878936BB
Show »

FASTA

425

47,685

        10         20         30         40         50         60 
MAPITTSRVE FDEIPTVVGI FSAFGLVFTV SLFAWICCQR RSAKSNKTPP YKFVHVLKGV 

        70         80         90        100        110        120 
DIYPENLSSK KKFGGDDKSE AKRKAALPNL SLHLDLEKRD LNGNFPKTNP KAGSSSDLEN 

       130        140        150        160        170        180 
VTPKLFPETE KEAVSPESLK SSTSLTSEEK QEKLGTLFLS LEYNFEKKAF VVNIKEAQGL 

       190        200        210        220        230        240 
PAMDEQSMTS DPYIKMTILP EKKHKVKTRV LRKTLDPVFD ETFTFYGVPY PHIQELSLHF 

       250        260        270        280        290        300 
TVLSFDRFSR DDVIGEVLVP LSGIELSDGK MLMTREIIKR NAKKSSGRGE LLVSLCYQST 

       310        320        330        340        350        360 
TNTLTVVVLK ARHLPKSDVS GLSDPYVKVN LYHAKKRISK KKTHVKKCTP NAVFNELFVF 

       370        380        390        400        410        420 
DIPCESLEEI SVEFLVLDSE RGSRNEVIGR LVLGATAEGS GGGHWKEICD FPRRQIAKWH 


MLCDG

« Hide

References

[1]	"Functional properties of multiple synaptotagmins in brain." Ullrich B., Li C., Zhang J.Z., McMahon H., Anderson R.G., Geppert M., Suedhof T.C. Neuron 13:1281-1291(1994) [PubMed: 7993622] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Synaptotagmin IV is an immediate early gene induced by depolarization in PC12 cells and in brain." Vician L., Lim I.K., Ferguson G., Tocco G., Baudry M., Herschman H.R. Proc. Natl. Acad. Sci. U.S.A. 92:2164-2168(1995) [PubMed: 7892240] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U14398 mRNA. Translation: AAA68519.1.
L38247 mRNA. Translation: AAA67327.1.

IPI

IPI00231989.

PIR

I59355.

RefSeq

NP_113881.1. NM_031693.2.

UniGene

Rn.126675.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1W15	X-ray	1.93	A	288-425	[»]
1W16	X-ray	2.30	A	288-425	[»]

ProteinModelPortal

P50232.

SMR

P50232. Positions 156-278, 288-425.

ModBase

Search...

Protein-protein interaction databases

IntAct

P50232. 2 interactions.

STRING

P50232.

PTM databases

PhosphoSite

P50232.

Proteomic databases

PRIDE

P50232.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSRNOT00000023594; ENSRNOP00000023593; ENSRNOG00000017333.

GeneID

64440.

KEGG

rno:64440.

UCSC

L38247. rat.

Organism-specific databases

CTD

6860.

RGD

68397. Syt4.

Phylogenomic databases

eggNOG

roNOG11953.

HOVERGEN

HBG005010.

InParanoid

P50232.

OMA

ENVTPKL.

OrthoDB

EOG4GMTX0.

PhylomeDB

P50232.

Gene expression databases

ArrayExpress

P50232.

Genevestigator

P50232.

GermOnline

ENSRNOG00000017333. Rattus norvegicus.

Family and domain databases

InterPro

IPR000008. C2_Ca-dep.
IPR008973. C2_Ca/lipid-bd_dom_CaLB.
IPR018029. C2_membr_targeting.
IPR001565. Synaptotagmin.
[Graphical view]

Pfam

PF00168. C2. 2 hits.
[Graphical view]

PRINTS

PR00399. SYNAPTOTAGMN.

SMART

SM00239. C2. 2 hits.
[Graphical view]

SUPFAM

SSF49562. C2_CaLB. 2 hits.

PROSITE

PS50004. C2. 2 hits.
[Graphical view]

ProtoNet

Search...

Other

NextBio

613158.

Entry information

Entry name

SYT4_RAT

Accession

Primary (citable) accession number: P50232

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	September 21, 2011
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents