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P50229

- CCL3_RAT

UniProt

P50229 - CCL3_RAT

Protein

C-C motif chemokine 3

Gene

Ccl3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Monokine with inflammatory and chemokinetic properties. Has chemotactic activity for monocytes, neutrophils, eosinophils, basophils, and lymphocytes. Required for lung TNF-alpha production, neutrophil recruitment and subsequent lung injury and may function as an autocrine mediator for the macrophage production of TNF-alpha which in turn up-regulates vascular adhesion molecules required for neutrophil influx. This protein binds heparin.

    GO - Molecular functioni

    1. calcium-dependent protein kinase C activity Source: UniProtKB
    2. chemoattractant activity Source: UniProtKB
    3. chemokine activity Source: RGD
    4. heparin binding Source: UniProtKB-KW
    5. kinase activity Source: UniProtKB
    6. phospholipase activator activity Source: UniProtKB
    7. protein kinase activity Source: UniProtKB

    GO - Biological processi

    1. astrocyte cell migration Source: UniProtKB
    2. behavior Source: UniProtKB
    3. calcium ion transport Source: UniProtKB
    4. calcium-mediated signaling Source: UniProtKB
    5. cell activation Source: UniProtKB
    6. cell-cell signaling Source: UniProtKB
    7. cellular calcium ion homeostasis Source: UniProtKB
    8. cellular response to organic cyclic compound Source: UniProtKB
    9. chemotaxis Source: UniProtKB
    10. cytoskeleton organization Source: UniProtKB
    11. eosinophil chemotaxis Source: UniProtKB
    12. eosinophil degranulation Source: UniProtKB
    13. exocytosis Source: UniProtKB
    14. granulocyte chemotaxis Source: UniProtKB
    15. inflammatory response Source: RGD
    16. leukocyte chemotaxis Source: RGD
    17. lipopolysaccharide-mediated signaling pathway Source: UniProtKB
    18. lymphocyte chemotaxis Source: UniProtKB
    19. macrophage chemotaxis Source: UniProtKB
    20. MAPK cascade Source: UniProtKB
    21. monocyte chemotaxis Source: UniProtKB
    22. negative regulation by host of viral transcription Source: UniProtKB
    23. negative regulation of gene expression Source: UniProtKB
    24. negative regulation of osteoclast differentiation Source: UniProtKB
    25. neutrophil chemotaxis Source: RGD
    26. osteoblast differentiation Source: UniProtKB
    27. positive chemotaxis Source: GOC
    28. positive regulation of calcium ion transport Source: UniProtKB
    29. positive regulation of calcium-mediated signaling Source: UniProtKB
    30. positive regulation of catalytic activity Source: GOC
    31. positive regulation of cell migration Source: UniProtKB
    32. positive regulation of cytosolic calcium ion concentration Source: RGD
    33. positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
    34. positive regulation of gene expression Source: UniProtKB
    35. positive regulation of inflammatory response Source: UniProtKB
    36. positive regulation of interleukin-1 beta secretion Source: UniProtKB
    37. positive regulation of natural killer cell chemotaxis Source: UniProtKB
    38. positive regulation of neuron apoptotic process Source: UniProtKB
    39. positive regulation of osteoclast differentiation Source: RGD
    40. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
    41. positive regulation of tumor necrosis factor production Source: UniProtKB
    42. protein kinase B signaling Source: UniProtKB
    43. protein phosphorylation Source: UniProtKB
    44. regulation of cell shape Source: UniProtKB
    45. regulation of sensory perception of pain Source: UniProtKB
    46. release of sequestered calcium ion into cytosol by sarcoplasmic reticulum Source: UniProtKB
    47. response to cholesterol Source: UniProtKB
    48. response to drug Source: RGD
    49. response to toxic substance Source: UniProtKB
    50. signaling Source: UniProtKB
    51. T cell chemotaxis Source: UniProtKB

    Keywords - Molecular functioni

    Cytokine

    Keywords - Biological processi

    Chemotaxis, Inflammatory response

    Keywords - Ligandi

    Heparin-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    C-C motif chemokine 3
    Alternative name(s):
    Macrophage inflammatory protein 1-alpha
    Short name:
    MIP-1-alpha
    Small-inducible cytokine A3
    Gene namesi
    Name:Ccl3
    Synonyms:Mip1a, Scya3
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 10

    Organism-specific databases

    RGDi3647. Ccl3.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. cytosol Source: UniProtKB
    3. extracellular space Source: UniProtKB
    4. intracellular Source: UniProtKB

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 23231 PublicationAdd
    BLAST
    Chaini24 – 9269C-C motif chemokine 3PRO_0000005160Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi34 ↔ 57By similarity
    Disulfide bondi35 ↔ 73By similarity

    Keywords - PTMi

    Disulfide bond

    Expressioni

    Inductioni

    By lipopolysaccharide (LPS).

    Gene expression databases

    GenevestigatoriP50229.

    Structurei

    3D structure databases

    ProteinModelPortaliP50229.
    SMRiP50229. Positions 24-91.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG42742.
    GeneTreeiENSGT00730000110278.
    HOGENOMiHOG000036685.
    HOVERGENiHBG017871.
    InParanoidiP50229.
    KOiK05408.
    OMAiLCAMALC.
    OrthoDBiEOG7CVQ1F.
    PhylomeDBiP50229.
    TreeFamiTF334888.

    Family and domain databases

    InterProiIPR000827. Chemokine_CC_CS.
    IPR001811. Chemokine_IL8-like_dom.
    [Graphical view]
    PfamiPF00048. IL8. 1 hit.
    [Graphical view]
    SMARTiSM00199. SCY. 1 hit.
    [Graphical view]
    SUPFAMiSSF54117. SSF54117. 1 hit.
    PROSITEiPS00472. SMALL_CYTOKINES_CC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P50229-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKVSTAALAV LLCTMALWNE VFSAPYGADT PTACCFSYGR QIPRKFIADY   50
    FETSSLCSQP GVIFLTKRNR QICADPKETW VQEYITELEL NA 92
    Length:92
    Mass (Da):10,335
    Last modified:October 1, 1996 - v1
    Checksum:i14E861C647F9A2EB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti6 – 61A → T in AAA96498. (PubMed:7722328)Curated
    Sequence conflicti57 – 571C → W in AAA96498. (PubMed:7722328)Curated
    Sequence conflicti57 – 571C → W AA sequence (PubMed:8607872)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U22414 mRNA. Translation: AAA80608.1.
    U06435 mRNA. Translation: AAA96498.1.
    EF121994 mRNA. Translation: ABL63433.1.
    EF121995 mRNA. Translation: ABL63434.1.
    PIRiI52322.
    RefSeqiNP_037157.2. NM_013025.2.
    UniGeneiRn.10139.

    Genome annotation databases

    EnsembliENSRNOT00000015139; ENSRNOP00000015139; ENSRNOG00000011205.
    GeneIDi25542.
    KEGGirno:25542.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U22414 mRNA. Translation: AAA80608.1 .
    U06435 mRNA. Translation: AAA96498.1 .
    EF121994 mRNA. Translation: ABL63433.1 .
    EF121995 mRNA. Translation: ABL63434.1 .
    PIRi I52322.
    RefSeqi NP_037157.2. NM_013025.2.
    UniGenei Rn.10139.

    3D structure databases

    ProteinModelPortali P50229.
    SMRi P50229. Positions 24-91.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000015139 ; ENSRNOP00000015139 ; ENSRNOG00000011205 .
    GeneIDi 25542.
    KEGGi rno:25542.

    Organism-specific databases

    CTDi 6348.
    RGDi 3647. Ccl3.

    Phylogenomic databases

    eggNOGi NOG42742.
    GeneTreei ENSGT00730000110278.
    HOGENOMi HOG000036685.
    HOVERGENi HBG017871.
    InParanoidi P50229.
    KOi K05408.
    OMAi LCAMALC.
    OrthoDBi EOG7CVQ1F.
    PhylomeDBi P50229.
    TreeFami TF334888.

    Miscellaneous databases

    NextBioi 607069.
    PROi P50229.

    Gene expression databases

    Genevestigatori P50229.

    Family and domain databases

    InterProi IPR000827. Chemokine_CC_CS.
    IPR001811. Chemokine_IL8-like_dom.
    [Graphical view ]
    Pfami PF00048. IL8. 1 hit.
    [Graphical view ]
    SMARTi SM00199. SCY. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54117. SSF54117. 1 hit.
    PROSITEi PS00472. SMALL_CYTOKINES_CC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and posttranscriptional regulation of macrophage inflammatory protein-1 alpha in alveolar macrophages."
      Shi M.M., Godleski J.J., Paulauskis J.D.
      Biochem. Biophys. Res. Commun. 211:289-295(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Lung.
    2. "Role of macrophage inflammatory protein-1 alpha (MIP-1 alpha) in acute lung injury in rats."
      Shanley T.P., Schmal H., Friedl H.P., Jones M.L., Ward P.A.
      J. Immunol. 154:4793-4802(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Long Evans.
      Tissue: Lung.
    3. "Fine-mapping and comprehensive transcript analysis reveals nonsynonymous variants within a novel 1.17 Mb blood pressure QTL region on rat chromosome 10."
      Saad Y., Garrett M.R., Manickavasagam E., Yerga-Woolwine S., Farms P., Radecki T., Joe B.
      Genomics 89:343-353(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Dahl salt-sensitive and Lewis.
    4. "Cytokine-induced neutrophil chemoattractant (CINC)-2 alpha, a novel member of rat GRO/CINCs, is a predominant chemokine produced by lipopolysaccharide-stimulated rat macrophages in culture."
      Nakagawa H., Shiota S., Takano K., Shibata F., Kato H.
      Biochem. Biophys. Res. Commun. 220:945-948(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 24-57.
      Strain: Wistar.

    Entry informationi

    Entry nameiCCL3_RAT
    AccessioniPrimary (citable) accession number: P50229
    Secondary accession number(s): A1EC87
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3