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Protein

C-C motif chemokine 3

Gene

Ccl3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Monokine with inflammatory and chemokinetic properties. Has chemotactic activity for monocytes, neutrophils, eosinophils, basophils, and lymphocytes. Required for lung TNF-alpha production, neutrophil recruitment and subsequent lung injury and may function as an autocrine mediator for the macrophage production of TNF-alpha which in turn up-regulates vascular adhesion molecules required for neutrophil influx. This protein binds heparin.

GO - Molecular functioni

  1. calcium-dependent protein kinase C activity Source: UniProtKB
  2. chemoattractant activity Source: UniProtKB
  3. chemokine activity Source: RGD
  4. heparin binding Source: UniProtKB-KW
  5. kinase activity Source: UniProtKB
  6. phospholipase activator activity Source: UniProtKB
  7. protein kinase activity Source: UniProtKB

GO - Biological processi

  1. astrocyte cell migration Source: UniProtKB
  2. behavior Source: UniProtKB
  3. calcium ion transport Source: UniProtKB
  4. calcium-mediated signaling Source: UniProtKB
  5. cell activation Source: UniProtKB
  6. cell-cell signaling Source: UniProtKB
  7. cellular calcium ion homeostasis Source: UniProtKB
  8. cellular response to organic cyclic compound Source: UniProtKB
  9. chemotaxis Source: UniProtKB
  10. cytoskeleton organization Source: UniProtKB
  11. eosinophil chemotaxis Source: UniProtKB
  12. eosinophil degranulation Source: UniProtKB
  13. exocytosis Source: UniProtKB
  14. granulocyte chemotaxis Source: UniProtKB
  15. inflammatory response Source: RGD
  16. leukocyte chemotaxis Source: RGD
  17. lipopolysaccharide-mediated signaling pathway Source: UniProtKB
  18. lymphocyte chemotaxis Source: UniProtKB
  19. macrophage chemotaxis Source: UniProtKB
  20. MAPK cascade Source: UniProtKB
  21. monocyte chemotaxis Source: UniProtKB
  22. negative regulation by host of viral transcription Source: UniProtKB
  23. negative regulation of gene expression Source: UniProtKB
  24. negative regulation of osteoclast differentiation Source: UniProtKB
  25. neutrophil chemotaxis Source: RGD
  26. osteoblast differentiation Source: UniProtKB
  27. positive chemotaxis Source: GOC
  28. positive regulation of calcium ion transport Source: UniProtKB
  29. positive regulation of calcium-mediated signaling Source: UniProtKB
  30. positive regulation of catalytic activity Source: GOC
  31. positive regulation of cell migration Source: UniProtKB
  32. positive regulation of cytosolic calcium ion concentration Source: RGD
  33. positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
  34. positive regulation of gene expression Source: UniProtKB
  35. positive regulation of inflammatory response Source: UniProtKB
  36. positive regulation of interleukin-1 beta secretion Source: UniProtKB
  37. positive regulation of natural killer cell chemotaxis Source: UniProtKB
  38. positive regulation of neuron apoptotic process Source: UniProtKB
  39. positive regulation of osteoclast differentiation Source: RGD
  40. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
  41. positive regulation of tumor necrosis factor production Source: UniProtKB
  42. protein kinase B signaling Source: UniProtKB
  43. protein phosphorylation Source: UniProtKB
  44. regulation of cell shape Source: UniProtKB
  45. regulation of sensory perception of pain Source: UniProtKB
  46. release of sequestered calcium ion into cytosol by sarcoplasmic reticulum Source: UniProtKB
  47. response to cholesterol Source: UniProtKB
  48. response to drug Source: RGD
  49. response to toxic substance Source: UniProtKB
  50. signaling Source: UniProtKB
  51. T cell chemotaxis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Cytokine

Keywords - Biological processi

Chemotaxis, Inflammatory response

Keywords - Ligandi

Heparin-binding

Enzyme and pathway databases

ReactomeiREACT_263785. Chemokine receptors bind chemokines.

Names & Taxonomyi

Protein namesi
Recommended name:
C-C motif chemokine 3
Alternative name(s):
Macrophage inflammatory protein 1-alpha
Short name:
MIP-1-alpha
Small-inducible cytokine A3
Gene namesi
Name:Ccl3
Synonyms:Mip1a, Scya3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 10

Organism-specific databases

RGDi3647. Ccl3.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. cytosol Source: UniProtKB
  3. extracellular space Source: UniProtKB
  4. intracellular Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 23231 PublicationAdd
BLAST
Chaini24 – 9269C-C motif chemokine 3PRO_0000005160Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi34 ↔ 57By similarity
Disulfide bondi35 ↔ 73By similarity

Keywords - PTMi

Disulfide bond

Expressioni

Inductioni

By lipopolysaccharide (LPS).

Gene expression databases

GenevestigatoriP50229.

Structurei

3D structure databases

ProteinModelPortaliP50229.
SMRiP50229. Positions 24-91.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG42742.
GeneTreeiENSGT00730000110278.
HOGENOMiHOG000036685.
HOVERGENiHBG017871.
InParanoidiP50229.
KOiK05408.
OMAiSAPFGAD.
OrthoDBiEOG7CVQ1F.
PhylomeDBiP50229.
TreeFamiTF334888.

Family and domain databases

InterProiIPR000827. Chemokine_CC_CS.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]
PfamiPF00048. IL8. 1 hit.
[Graphical view]
SMARTiSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMiSSF54117. SSF54117. 1 hit.
PROSITEiPS00472. SMALL_CYTOKINES_CC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50229-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVSTAALAV LLCTMALWNE VFSAPYGADT PTACCFSYGR QIPRKFIADY
60 70 80 90
FETSSLCSQP GVIFLTKRNR QICADPKETW VQEYITELEL NA
Length:92
Mass (Da):10,335
Last modified:October 1, 1996 - v1
Checksum:i14E861C647F9A2EB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti6 – 61A → T in AAA96498 (PubMed:7722328).Curated
Sequence conflicti57 – 571C → W in AAA96498 (PubMed:7722328).Curated
Sequence conflicti57 – 571C → W AA sequence (PubMed:8607872).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22414 mRNA. Translation: AAA80608.1.
U06435 mRNA. Translation: AAA96498.1.
EF121994 mRNA. Translation: ABL63433.1.
EF121995 mRNA. Translation: ABL63434.1.
PIRiI52322.
RefSeqiNP_037157.2. NM_013025.2.
UniGeneiRn.10139.

Genome annotation databases

EnsembliENSRNOT00000015139; ENSRNOP00000015139; ENSRNOG00000011205.
GeneIDi25542.
KEGGirno:25542.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22414 mRNA. Translation: AAA80608.1.
U06435 mRNA. Translation: AAA96498.1.
EF121994 mRNA. Translation: ABL63433.1.
EF121995 mRNA. Translation: ABL63434.1.
PIRiI52322.
RefSeqiNP_037157.2. NM_013025.2.
UniGeneiRn.10139.

3D structure databases

ProteinModelPortaliP50229.
SMRiP50229. Positions 24-91.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000015139; ENSRNOP00000015139; ENSRNOG00000011205.
GeneIDi25542.
KEGGirno:25542.

Organism-specific databases

CTDi6348.
RGDi3647. Ccl3.

Phylogenomic databases

eggNOGiNOG42742.
GeneTreeiENSGT00730000110278.
HOGENOMiHOG000036685.
HOVERGENiHBG017871.
InParanoidiP50229.
KOiK05408.
OMAiSAPFGAD.
OrthoDBiEOG7CVQ1F.
PhylomeDBiP50229.
TreeFamiTF334888.

Enzyme and pathway databases

ReactomeiREACT_263785. Chemokine receptors bind chemokines.

Miscellaneous databases

NextBioi607069.
PROiP50229.

Gene expression databases

GenevestigatoriP50229.

Family and domain databases

InterProiIPR000827. Chemokine_CC_CS.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]
PfamiPF00048. IL8. 1 hit.
[Graphical view]
SMARTiSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMiSSF54117. SSF54117. 1 hit.
PROSITEiPS00472. SMALL_CYTOKINES_CC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and posttranscriptional regulation of macrophage inflammatory protein-1 alpha in alveolar macrophages."
    Shi M.M., Godleski J.J., Paulauskis J.D.
    Biochem. Biophys. Res. Commun. 211:289-295(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Lung.
  2. "Role of macrophage inflammatory protein-1 alpha (MIP-1 alpha) in acute lung injury in rats."
    Shanley T.P., Schmal H., Friedl H.P., Jones M.L., Ward P.A.
    J. Immunol. 154:4793-4802(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Long Evans.
    Tissue: Lung.
  3. "Fine-mapping and comprehensive transcript analysis reveals nonsynonymous variants within a novel 1.17 Mb blood pressure QTL region on rat chromosome 10."
    Saad Y., Garrett M.R., Manickavasagam E., Yerga-Woolwine S., Farms P., Radecki T., Joe B.
    Genomics 89:343-353(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Dahl salt-sensitive and Lewis.
  4. "Cytokine-induced neutrophil chemoattractant (CINC)-2 alpha, a novel member of rat GRO/CINCs, is a predominant chemokine produced by lipopolysaccharide-stimulated rat macrophages in culture."
    Nakagawa H., Shiota S., Takano K., Shibata F., Kato H.
    Biochem. Biophys. Res. Commun. 220:945-948(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 24-57.
    Strain: Wistar.

Entry informationi

Entry nameiCCL3_RAT
AccessioniPrimary (citable) accession number: P50229
Secondary accession number(s): A1EC87
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: January 7, 2015
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.