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P50229 (CCL3_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
C-C motif chemokine 3
Alternative name(s):
Macrophage inflammatory protein 1-alpha
Short name=MIP-1-alpha
Small-inducible cytokine A3
Gene names
Name:Ccl3
Synonyms:Mip1a, Scya3
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length92 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Monokine with inflammatory and chemokinetic properties. Has chemotactic activity for monocytes, neutrophils, eosinophils, basophils, and lymphocytes. Required for lung TNF-alpha production, neutrophil recruitment and subsequent lung injury and may function as an autocrine mediator for the macrophage production of TNF-alpha which in turn up-regulates vascular adhesion molecules required for neutrophil influx. This protein binds heparin.

Subcellular location

Secreted.

Induction

By lipopolysaccharide (LPS).

Sequence similarities

Belongs to the intercrine beta (chemokine CC) family.

Ontologies

Keywords
   Biological processChemotaxis
Inflammatory response
   Cellular componentSecreted
   DomainSignal
   LigandHeparin-binding
   Molecular functionCytokine
   PTMDisulfide bond
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processMAPK cascade

Inferred from sequence or structural similarity. Source: UniProtKB

T cell chemotaxis

Inferred from sequence or structural similarity. Source: UniProtKB

astrocyte cell migration

Inferred from sequence or structural similarity. Source: UniProtKB

behavior

Inferred from sequence or structural similarity. Source: UniProtKB

calcium ion transport

Inferred from sequence or structural similarity. Source: UniProtKB

calcium-mediated signaling

Inferred from sequence or structural similarity. Source: UniProtKB

cell activation

Inferred from sequence or structural similarity. Source: UniProtKB

cell-cell signaling

Inferred from sequence or structural similarity. Source: UniProtKB

cellular calcium ion homeostasis

Inferred from sequence or structural similarity. Source: UniProtKB

cellular response to organic cyclic compound

Inferred from sequence or structural similarity. Source: UniProtKB

chemotaxis

Inferred from sequence or structural similarity. Source: UniProtKB

cytoskeleton organization

Inferred from sequence or structural similarity. Source: UniProtKB

eosinophil chemotaxis

Inferred from sequence or structural similarity. Source: UniProtKB

eosinophil degranulation

Inferred from sequence or structural similarity. Source: UniProtKB

exocytosis

Inferred from sequence or structural similarity. Source: UniProtKB

granulocyte chemotaxis

Inferred from sequence or structural similarity. Source: UniProtKB

inflammatory response

Inferred from expression pattern PubMed 12144807. Source: RGD

leukocyte chemotaxis

Inferred from direct assay Ref.2. Source: RGD

lipopolysaccharide-mediated signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

lymphocyte chemotaxis

Inferred from sequence or structural similarity. Source: UniProtKB

macrophage chemotaxis

Inferred from sequence or structural similarity. Source: UniProtKB

monocyte chemotaxis

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation by host of viral transcription

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of gene expression

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of osteoclast differentiation

Inferred from sequence or structural similarity. Source: UniProtKB

neutrophil chemotaxis

Inferred from direct assay PubMed 10466578Ref.2. Source: RGD

osteoblast differentiation

Inferred from sequence or structural similarity. Source: UniProtKB

positive chemotaxis

Inferred from sequence or structural similarity. Source: GOC

positive regulation of ERK1 and ERK2 cascade

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of calcium ion transport

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of calcium-mediated signaling

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of catalytic activity

Inferred from sequence or structural similarity. Source: GOC

positive regulation of cell migration

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of cytosolic calcium ion concentration

Inferred from direct assay PubMed 10466578. Source: RGD

positive regulation of gene expression

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of inflammatory response

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of interleukin-1 beta secretion

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of natural killer cell chemotaxis

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of neuron apoptotic process

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of osteoclast differentiation

Inferred from direct assay PubMed 9920817. Source: RGD

positive regulation of transcription from RNA polymerase II promoter

Inferred from electronic annotation. Source: Ensembl

positive regulation of tumor necrosis factor production

Inferred from sequence or structural similarity. Source: UniProtKB

protein kinase B signaling

Inferred from sequence or structural similarity. Source: UniProtKB

protein phosphorylation

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of cell shape

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of sensory perception of pain

Inferred from sequence or structural similarity. Source: UniProtKB

release of sequestered calcium ion into cytosol by sarcoplasmic reticulum

Inferred from sequence or structural similarity. Source: UniProtKB

response to cholesterol

Inferred from sequence or structural similarity. Source: UniProtKB

response to drug

Inferred from expression pattern PubMed 17666800. Source: RGD

response to toxic substance

Inferred from sequence or structural similarity. Source: UniProtKB

signaling

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentcytoplasm

Inferred from sequence or structural similarity. Source: UniProtKB

cytosol

Inferred from sequence or structural similarity. Source: UniProtKB

extracellular space

Inferred from sequence or structural similarity. Source: UniProtKB

intracellular

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functioncalcium-dependent protein kinase C activity

Inferred from sequence or structural similarity. Source: UniProtKB

chemoattractant activity

Inferred from sequence or structural similarity. Source: UniProtKB

chemokine activity

Inferred from direct assay PubMed 10466578. Source: RGD

heparin binding

Inferred from electronic annotation. Source: UniProtKB-KW

kinase activity

Inferred from sequence or structural similarity. Source: UniProtKB

phospholipase activator activity

Inferred from sequence or structural similarity. Source: UniProtKB

protein kinase activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Ref.4
Chain24 – 9269C-C motif chemokine 3
PRO_0000005160

Amino acid modifications

Disulfide bond34 ↔ 57 By similarity
Disulfide bond35 ↔ 73 By similarity

Experimental info

Sequence conflict61A → T in AAA96498. Ref.2
Sequence conflict571C → W in AAA96498. Ref.2
Sequence conflict571C → W AA sequence Ref.4

Sequences

Sequence LengthMass (Da)Tools
P50229 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 14E861C647F9A2EB

FASTA9210,335
        10         20         30         40         50         60 
MKVSTAALAV LLCTMALWNE VFSAPYGADT PTACCFSYGR QIPRKFIADY FETSSLCSQP 

        70         80         90 
GVIFLTKRNR QICADPKETW VQEYITELEL NA 

« Hide

References

[1]"Molecular cloning and posttranscriptional regulation of macrophage inflammatory protein-1 alpha in alveolar macrophages."
Shi M.M., Godleski J.J., Paulauskis J.D.
Biochem. Biophys. Res. Commun. 211:289-295(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Lung.
[2]"Role of macrophage inflammatory protein-1 alpha (MIP-1 alpha) in acute lung injury in rats."
Shanley T.P., Schmal H., Friedl H.P., Jones M.L., Ward P.A.
J. Immunol. 154:4793-4802(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Long Evans.
Tissue: Lung.
[3]"Fine-mapping and comprehensive transcript analysis reveals nonsynonymous variants within a novel 1.17 Mb blood pressure QTL region on rat chromosome 10."
Saad Y., Garrett M.R., Manickavasagam E., Yerga-Woolwine S., Farms P., Radecki T., Joe B.
Genomics 89:343-353(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Dahl salt-sensitive and Lewis.
[4]"Cytokine-induced neutrophil chemoattractant (CINC)-2 alpha, a novel member of rat GRO/CINCs, is a predominant chemokine produced by lipopolysaccharide-stimulated rat macrophages in culture."
Nakagawa H., Shiota S., Takano K., Shibata F., Kato H.
Biochem. Biophys. Res. Commun. 220:945-948(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 24-57.
Strain: Wistar.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U22414 mRNA. Translation: AAA80608.1.
U06435 mRNA. Translation: AAA96498.1.
EF121994 mRNA. Translation: ABL63433.1.
EF121995 mRNA. Translation: ABL63434.1.
PIRI52322.
RefSeqNP_037157.2. NM_013025.2.
UniGeneRn.10139.

3D structure databases

ProteinModelPortalP50229.
SMRP50229. Positions 24-91.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000015139; ENSRNOP00000015139; ENSRNOG00000011205.
GeneID25542.
KEGGrno:25542.

Organism-specific databases

CTD6348.
RGD3647. Ccl3.

Phylogenomic databases

eggNOGNOG42742.
GeneTreeENSGT00730000110278.
HOGENOMHOG000036685.
HOVERGENHBG017871.
InParanoidP50229.
KOK05408.
OMASAPFGAD.
OrthoDBEOG7CVQ1F.
PhylomeDBP50229.
TreeFamTF334888.

Gene expression databases

GenevestigatorP50229.

Family and domain databases

InterProIPR000827. Chemokine_CC_CS.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]
PfamPF00048. IL8. 1 hit.
[Graphical view]
SMARTSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMSSF54117. SSF54117. 1 hit.
PROSITEPS00472. SMALL_CYTOKINES_CC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio607069.
PROP50229.

Entry information

Entry nameCCL3_RAT
AccessionPrimary (citable) accession number: P50229
Secondary accession number(s): A1EC87
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families