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P50229

- CCL3_RAT

UniProt

P50229 - CCL3_RAT

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Protein

C-C motif chemokine 3

Gene

Ccl3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Monokine with inflammatory and chemokinetic properties. Has chemotactic activity for monocytes, neutrophils, eosinophils, basophils, and lymphocytes. Required for lung TNF-alpha production, neutrophil recruitment and subsequent lung injury and may function as an autocrine mediator for the macrophage production of TNF-alpha which in turn up-regulates vascular adhesion molecules required for neutrophil influx. This protein binds heparin.

GO - Molecular functioni

  1. calcium-dependent protein kinase C activity Source: UniProtKB
  2. chemoattractant activity Source: UniProtKB
  3. chemokine activity Source: RGD
  4. heparin binding Source: UniProtKB-KW
  5. kinase activity Source: UniProtKB
  6. phospholipase activator activity Source: UniProtKB
  7. protein kinase activity Source: UniProtKB

GO - Biological processi

  1. astrocyte cell migration Source: UniProtKB
  2. behavior Source: UniProtKB
  3. calcium ion transport Source: UniProtKB
  4. calcium-mediated signaling Source: UniProtKB
  5. cell activation Source: UniProtKB
  6. cell-cell signaling Source: UniProtKB
  7. cellular calcium ion homeostasis Source: UniProtKB
  8. cellular response to organic cyclic compound Source: UniProtKB
  9. chemotaxis Source: UniProtKB
  10. cytoskeleton organization Source: UniProtKB
  11. eosinophil chemotaxis Source: UniProtKB
  12. eosinophil degranulation Source: UniProtKB
  13. exocytosis Source: UniProtKB
  14. granulocyte chemotaxis Source: UniProtKB
  15. inflammatory response Source: RGD
  16. leukocyte chemotaxis Source: RGD
  17. lipopolysaccharide-mediated signaling pathway Source: UniProtKB
  18. lymphocyte chemotaxis Source: UniProtKB
  19. macrophage chemotaxis Source: UniProtKB
  20. MAPK cascade Source: UniProtKB
  21. monocyte chemotaxis Source: UniProtKB
  22. negative regulation by host of viral transcription Source: UniProtKB
  23. negative regulation of gene expression Source: UniProtKB
  24. negative regulation of osteoclast differentiation Source: UniProtKB
  25. neutrophil chemotaxis Source: RGD
  26. osteoblast differentiation Source: UniProtKB
  27. positive chemotaxis Source: GOC
  28. positive regulation of calcium ion transport Source: UniProtKB
  29. positive regulation of calcium-mediated signaling Source: UniProtKB
  30. positive regulation of catalytic activity Source: GOC
  31. positive regulation of cell migration Source: UniProtKB
  32. positive regulation of cytosolic calcium ion concentration Source: RGD
  33. positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
  34. positive regulation of gene expression Source: UniProtKB
  35. positive regulation of inflammatory response Source: UniProtKB
  36. positive regulation of interleukin-1 beta secretion Source: UniProtKB
  37. positive regulation of natural killer cell chemotaxis Source: UniProtKB
  38. positive regulation of neuron apoptotic process Source: UniProtKB
  39. positive regulation of osteoclast differentiation Source: RGD
  40. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
  41. positive regulation of tumor necrosis factor production Source: UniProtKB
  42. protein kinase B signaling Source: UniProtKB
  43. protein phosphorylation Source: UniProtKB
  44. regulation of cell shape Source: UniProtKB
  45. regulation of sensory perception of pain Source: UniProtKB
  46. release of sequestered calcium ion into cytosol by sarcoplasmic reticulum Source: UniProtKB
  47. response to cholesterol Source: UniProtKB
  48. response to drug Source: RGD
  49. response to toxic substance Source: UniProtKB
  50. signaling Source: UniProtKB
  51. T cell chemotaxis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Cytokine

Keywords - Biological processi

Chemotaxis, Inflammatory response

Keywords - Ligandi

Heparin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
C-C motif chemokine 3
Alternative name(s):
Macrophage inflammatory protein 1-alpha
Short name:
MIP-1-alpha
Small-inducible cytokine A3
Gene namesi
Name:Ccl3
Synonyms:Mip1a, Scya3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 10

Organism-specific databases

RGDi3647. Ccl3.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. cytosol Source: UniProtKB
  3. extracellular space Source: UniProtKB
  4. intracellular Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 23231 PublicationAdd
BLAST
Chaini24 – 9269C-C motif chemokine 3PRO_0000005160Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi34 ↔ 57By similarity
Disulfide bondi35 ↔ 73By similarity

Keywords - PTMi

Disulfide bond

Expressioni

Inductioni

By lipopolysaccharide (LPS).

Gene expression databases

GenevestigatoriP50229.

Structurei

3D structure databases

ProteinModelPortaliP50229.
SMRiP50229. Positions 24-91.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG42742.
GeneTreeiENSGT00730000110278.
HOGENOMiHOG000036685.
HOVERGENiHBG017871.
InParanoidiP50229.
KOiK05408.
OMAiLCAMALC.
OrthoDBiEOG7CVQ1F.
PhylomeDBiP50229.
TreeFamiTF334888.

Family and domain databases

InterProiIPR000827. Chemokine_CC_CS.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]
PfamiPF00048. IL8. 1 hit.
[Graphical view]
SMARTiSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMiSSF54117. SSF54117. 1 hit.
PROSITEiPS00472. SMALL_CYTOKINES_CC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50229 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKVSTAALAV LLCTMALWNE VFSAPYGADT PTACCFSYGR QIPRKFIADY
60 70 80 90
FETSSLCSQP GVIFLTKRNR QICADPKETW VQEYITELEL NA
Length:92
Mass (Da):10,335
Last modified:October 1, 1996 - v1
Checksum:i14E861C647F9A2EB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti6 – 61A → T in AAA96498. (PubMed:7722328)Curated
Sequence conflicti57 – 571C → W in AAA96498. (PubMed:7722328)Curated
Sequence conflicti57 – 571C → W AA sequence (PubMed:8607872)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U22414 mRNA. Translation: AAA80608.1.
U06435 mRNA. Translation: AAA96498.1.
EF121994 mRNA. Translation: ABL63433.1.
EF121995 mRNA. Translation: ABL63434.1.
PIRiI52322.
RefSeqiNP_037157.2. NM_013025.2.
UniGeneiRn.10139.

Genome annotation databases

EnsembliENSRNOT00000015139; ENSRNOP00000015139; ENSRNOG00000011205.
GeneIDi25542.
KEGGirno:25542.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U22414 mRNA. Translation: AAA80608.1 .
U06435 mRNA. Translation: AAA96498.1 .
EF121994 mRNA. Translation: ABL63433.1 .
EF121995 mRNA. Translation: ABL63434.1 .
PIRi I52322.
RefSeqi NP_037157.2. NM_013025.2.
UniGenei Rn.10139.

3D structure databases

ProteinModelPortali P50229.
SMRi P50229. Positions 24-91.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000015139 ; ENSRNOP00000015139 ; ENSRNOG00000011205 .
GeneIDi 25542.
KEGGi rno:25542.

Organism-specific databases

CTDi 6348.
RGDi 3647. Ccl3.

Phylogenomic databases

eggNOGi NOG42742.
GeneTreei ENSGT00730000110278.
HOGENOMi HOG000036685.
HOVERGENi HBG017871.
InParanoidi P50229.
KOi K05408.
OMAi LCAMALC.
OrthoDBi EOG7CVQ1F.
PhylomeDBi P50229.
TreeFami TF334888.

Miscellaneous databases

NextBioi 607069.
PROi P50229.

Gene expression databases

Genevestigatori P50229.

Family and domain databases

InterProi IPR000827. Chemokine_CC_CS.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view ]
Pfami PF00048. IL8. 1 hit.
[Graphical view ]
SMARTi SM00199. SCY. 1 hit.
[Graphical view ]
SUPFAMi SSF54117. SSF54117. 1 hit.
PROSITEi PS00472. SMALL_CYTOKINES_CC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and posttranscriptional regulation of macrophage inflammatory protein-1 alpha in alveolar macrophages."
    Shi M.M., Godleski J.J., Paulauskis J.D.
    Biochem. Biophys. Res. Commun. 211:289-295(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Lung.
  2. "Role of macrophage inflammatory protein-1 alpha (MIP-1 alpha) in acute lung injury in rats."
    Shanley T.P., Schmal H., Friedl H.P., Jones M.L., Ward P.A.
    J. Immunol. 154:4793-4802(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Long Evans.
    Tissue: Lung.
  3. "Fine-mapping and comprehensive transcript analysis reveals nonsynonymous variants within a novel 1.17 Mb blood pressure QTL region on rat chromosome 10."
    Saad Y., Garrett M.R., Manickavasagam E., Yerga-Woolwine S., Farms P., Radecki T., Joe B.
    Genomics 89:343-353(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Dahl salt-sensitive and Lewis.
  4. "Cytokine-induced neutrophil chemoattractant (CINC)-2 alpha, a novel member of rat GRO/CINCs, is a predominant chemokine produced by lipopolysaccharide-stimulated rat macrophages in culture."
    Nakagawa H., Shiota S., Takano K., Shibata F., Kato H.
    Biochem. Biophys. Res. Commun. 220:945-948(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 24-57.
    Strain: Wistar.

Entry informationi

Entry nameiCCL3_RAT
AccessioniPrimary (citable) accession number: P50229
Secondary accession number(s): A1EC87
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 1, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3