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Protein

C-C motif chemokine 3

Gene

Ccl3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Monokine with inflammatory and chemokinetic properties. Has chemotactic activity for monocytes, neutrophils, eosinophils, basophils, and lymphocytes. Required for lung TNF-alpha production, neutrophil recruitment and subsequent lung injury and may function as an autocrine mediator for the macrophage production of TNF-alpha which in turn up-regulates vascular adhesion molecules required for neutrophil influx. This protein binds heparin.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Cytokine

Keywords - Biological processi

Chemotaxis, Inflammatory response

Keywords - Ligandi

Heparin-binding

Enzyme and pathway databases

ReactomeiR-RNO-380108. Chemokine receptors bind chemokines.

Names & Taxonomyi

Protein namesi
Recommended name:
C-C motif chemokine 3
Alternative name(s):
Macrophage inflammatory protein 1-alpha
Short name:
MIP-1-alpha
Small-inducible cytokine A3
Gene namesi
Name:Ccl3
Synonyms:Mip1a, Scya3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi3647. Ccl3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 23231 PublicationAdd
BLAST
Chaini24 – 9269C-C motif chemokine 3PRO_0000005160Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi34 ↔ 57By similarity
Disulfide bondi35 ↔ 73By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP50229.

Expressioni

Inductioni

By lipopolysaccharide (LPS).

Gene expression databases

GenevisibleiP50229. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000015139.

Structurei

3D structure databases

ProteinModelPortaliP50229.
SMRiP50229. Positions 24-91.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410J4I9. Eukaryota.
ENOG410YXJU. LUCA.
GeneTreeiENSGT00730000110278.
HOGENOMiHOG000036685.
HOVERGENiHBG017871.
InParanoidiP50229.
KOiK05408.
OMAiSAPFGAD.
OrthoDBiEOG7CVQ1F.
PhylomeDBiP50229.
TreeFamiTF334888.

Family and domain databases

InterProiIPR000827. Chemokine_CC_CS.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]
PfamiPF00048. IL8. 1 hit.
[Graphical view]
SMARTiSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMiSSF54117. SSF54117. 1 hit.
PROSITEiPS00472. SMALL_CYTOKINES_CC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50229-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVSTAALAV LLCTMALWNE VFSAPYGADT PTACCFSYGR QIPRKFIADY
60 70 80 90
FETSSLCSQP GVIFLTKRNR QICADPKETW VQEYITELEL NA
Length:92
Mass (Da):10,335
Last modified:October 1, 1996 - v1
Checksum:i14E861C647F9A2EB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti6 – 61A → T in AAA96498 (PubMed:7722328).Curated
Sequence conflicti57 – 571C → W in AAA96498 (PubMed:7722328).Curated
Sequence conflicti57 – 571C → W AA sequence (PubMed:8607872).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22414 mRNA. Translation: AAA80608.1.
U06435 mRNA. Translation: AAA96498.1.
EF121994 mRNA. Translation: ABL63433.1.
EF121995 mRNA. Translation: ABL63434.1.
PIRiI52322.
RefSeqiNP_037157.2. NM_013025.2.
UniGeneiRn.10139.

Genome annotation databases

EnsembliENSRNOT00000015139; ENSRNOP00000015139; ENSRNOG00000011205.
GeneIDi25542.
KEGGirno:25542.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22414 mRNA. Translation: AAA80608.1.
U06435 mRNA. Translation: AAA96498.1.
EF121994 mRNA. Translation: ABL63433.1.
EF121995 mRNA. Translation: ABL63434.1.
PIRiI52322.
RefSeqiNP_037157.2. NM_013025.2.
UniGeneiRn.10139.

3D structure databases

ProteinModelPortaliP50229.
SMRiP50229. Positions 24-91.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000015139.

Proteomic databases

PaxDbiP50229.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000015139; ENSRNOP00000015139; ENSRNOG00000011205.
GeneIDi25542.
KEGGirno:25542.

Organism-specific databases

CTDi6348.
RGDi3647. Ccl3.

Phylogenomic databases

eggNOGiENOG410J4I9. Eukaryota.
ENOG410YXJU. LUCA.
GeneTreeiENSGT00730000110278.
HOGENOMiHOG000036685.
HOVERGENiHBG017871.
InParanoidiP50229.
KOiK05408.
OMAiSAPFGAD.
OrthoDBiEOG7CVQ1F.
PhylomeDBiP50229.
TreeFamiTF334888.

Enzyme and pathway databases

ReactomeiR-RNO-380108. Chemokine receptors bind chemokines.

Miscellaneous databases

NextBioi607069.
PROiP50229.

Gene expression databases

GenevisibleiP50229. RN.

Family and domain databases

InterProiIPR000827. Chemokine_CC_CS.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]
PfamiPF00048. IL8. 1 hit.
[Graphical view]
SMARTiSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMiSSF54117. SSF54117. 1 hit.
PROSITEiPS00472. SMALL_CYTOKINES_CC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and posttranscriptional regulation of macrophage inflammatory protein-1 alpha in alveolar macrophages."
    Shi M.M., Godleski J.J., Paulauskis J.D.
    Biochem. Biophys. Res. Commun. 211:289-295(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Lung.
  2. "Role of macrophage inflammatory protein-1 alpha (MIP-1 alpha) in acute lung injury in rats."
    Shanley T.P., Schmal H., Friedl H.P., Jones M.L., Ward P.A.
    J. Immunol. 154:4793-4802(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Long Evans.
    Tissue: Lung.
  3. "Fine-mapping and comprehensive transcript analysis reveals nonsynonymous variants within a novel 1.17 Mb blood pressure QTL region on rat chromosome 10."
    Saad Y., Garrett M.R., Manickavasagam E., Yerga-Woolwine S., Farms P., Radecki T., Joe B.
    Genomics 89:343-353(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Dahl salt-sensitive and Lewis.
  4. "Cytokine-induced neutrophil chemoattractant (CINC)-2 alpha, a novel member of rat GRO/CINCs, is a predominant chemokine produced by lipopolysaccharide-stimulated rat macrophages in culture."
    Nakagawa H., Shiota S., Takano K., Shibata F., Kato H.
    Biochem. Biophys. Res. Commun. 220:945-948(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 24-57.
    Strain: Wistar.

Entry informationi

Entry nameiCCL3_RAT
AccessioniPrimary (citable) accession number: P50229
Secondary accession number(s): A1EC87
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 11, 2015
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.