Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P50220

- NKX21_MOUSE

UniProt

P50220 - NKX21_MOUSE

Protein

Homeobox protein Nkx-2.1

Gene

Nkx2-1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. Activates the transcription of GNRHR and plays a role in enhancing the circadian oscillation of its gene expression. Represses the transcription of the circadian transcriptional repressor NR1D1.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi161 – 22060HomeoboxPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. core promoter binding Source: UniProtKB
    2. protein binding Source: MGI
    3. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Source: MGI
    4. sequence-specific DNA binding Source: MGI
    5. sequence-specific DNA binding transcription factor activity Source: MGI
    6. transcription regulatory region DNA binding Source: MGI

    GO - Biological processi

    1. anatomical structure formation involved in morphogenesis Source: MGI
    2. axon guidance Source: MGI
    3. brain development Source: MGI
    4. cerebral cortex cell migration Source: MGI
    5. cerebral cortex GABAergic interneuron differentiation Source: MGI
    6. cerebral cortex neuron differentiation Source: MGI
    7. Clara cell differentiation Source: MGI
    8. developmental induction Source: MGI
    9. endoderm development Source: MGI
    10. epithelial tube branching involved in lung morphogenesis Source: Ensembl
    11. forebrain dorsal/ventral pattern formation Source: MGI
    12. forebrain neuron differentiation Source: MGI
    13. forebrain neuron fate commitment Source: MGI
    14. globus pallidus development Source: MGI
    15. hippocampus development Source: MGI
    16. Leydig cell differentiation Source: MGI
    17. locomotory behavior Source: MGI
    18. lung development Source: MGI
    19. lung saccule development Source: MGI
    20. menarche Source: MGI
    21. negative regulation of cell migration Source: Ensembl
    22. negative regulation of epithelial to mesenchymal transition Source: Ensembl
    23. negative regulation of transcription, DNA-templated Source: UniProtKB
    24. negative regulation of transcription from RNA polymerase II promoter Source: MGI
    25. negative regulation of transforming growth factor beta receptor signaling pathway Source: Ensembl
    26. neuron fate commitment Source: MGI
    27. neuron migration Source: MGI
    28. oligodendrocyte differentiation Source: MGI
    29. organ morphogenesis Source: MGI
    30. pattern specification process Source: MGI
    31. phospholipid metabolic process Source: MGI
    32. pituitary gland development Source: MGI
    33. positive regulation of circadian rhythm Source: UniProtKB
    34. positive regulation of transcription, DNA-templated Source: UniProtKB
    35. positive regulation of transcription from RNA polymerase II promoter Source: MGI
    36. regulation of transcription, DNA-templated Source: MGI
    37. regulation of transcription from RNA polymerase II promoter Source: MGI
    38. response to hormone Source: Ensembl
    39. telencephalon cell migration Source: MGI
    40. telencephalon development Source: MGI
    41. thyroid gland development Source: MGI
    42. transcription from RNA polymerase II promoter Source: GOC
    43. Type II pneumocyte differentiation Source: MGI

    Keywords - Molecular functioni

    Activator, Repressor

    Keywords - Biological processi

    Biological rhythms, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Homeobox protein Nkx-2.1
    Alternative name(s):
    Thyroid nuclear factor 1
    Thyroid transcription factor 1
    Short name:
    TTF-1
    Thyroid-specific enhancer-binding protein
    Short name:
    T/EBP
    Gene namesi
    Name:Nkx2-1
    Synonyms:Nkx-2.1, Titf1, Ttf1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 12

    Organism-specific databases

    MGIiMGI:108067. Nkx2-1.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleoplasm Source: UniProtKB
    2. nucleus Source: MGI
    3. transcription factor complex Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 372372Homeobox protein Nkx-2.1PRO_0000049344Add
    BLAST

    Post-translational modificationi

    Phosphorylated on serine residues by STK3/MST2.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP50220.
    PRIDEiP50220.

    PTM databases

    PhosphoSiteiP50220.

    Expressioni

    Tissue specificityi

    Thyroid, lung and brain.

    Gene expression databases

    BgeeiP50220.
    CleanExiMM_NKX2-1.
    MM_TTF1.
    GenevestigatoriP50220.

    Interactioni

    Subunit structurei

    Interacts with WWTR1.By similarity

    Protein-protein interaction databases

    BioGridi204207. 12 interactions.
    STRINGi10090.ENSMUSP00000001536.

    Structurei

    3D structure databases

    ProteinModelPortaliP50220.
    SMRiP50220. Positions 161-227.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi234 – 24411Poly-GlyAdd
    BLAST
    Compositional biasi247 – 2548Poly-Gln
    Compositional biasi295 – 30410Poly-Ala

    Sequence similaritiesi

    Belongs to the NK-2 homeobox family.Curated
    Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Homeobox

    Phylogenomic databases

    eggNOGiNOG328614.
    GeneTreeiENSGT00720000108380.
    HOGENOMiHOG000231923.
    HOVERGENiHBG006689.
    InParanoidiP50220.
    KOiK09342.
    OMAiKSMAPLQ.
    OrthoDBiEOG769ZK0.
    PhylomeDBiP50220.
    TreeFamiTF351204.

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    InterProiIPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR020479. Homeobox_metazoa.
    IPR009057. Homeodomain-like.
    [Graphical view]
    PfamiPF00046. Homeobox. 1 hit.
    [Graphical view]
    PRINTSiPR00024. HOMEOBOX.
    SMARTiSM00389. HOX. 1 hit.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 1 hit.
    PROSITEiPS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P50220-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSMSPKHTTP FSVSDILSPL EESYKKVGME GGGLGAPLAA YRQGQAAPPA    50
    AAMQQHAVGH HGAVTAAYHM TAAGVPQLSH SAVGGYCNGN LGNMSELPPY 100
    QDTMRNSASG PGWYGANPDP RFPAISRFMG PASGMNMSGM GGLGSLGDVS 150
    KNMAPLPSAP RRKRRVLFSQ AQVYELERRF KQQKYLSAPE REHLASMIHL 200
    TPTQVKIWFQ NHRYKMKRQA KDKAAQQQLQ QDSGGGGGGG GGAGCPQQQQ 250
    AQQQSPRRVA VPVLVKDGKP CQAGAPAPGA ASLQSHAQQQ AQQQAQAAQA 300
    AAAAISVGSG GAGLGAHPGH QPGSAGQSPD LAHHAASPAG LQGQVSSLSH 350
    LNSSGSDYGA MSCSTLLYGR TW 372
    Length:372
    Mass (Da):38,570
    Last modified:October 1, 1996 - v1
    Checksum:i377B5CE9B709412F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U19755 Genomic DNA. Translation: AAA86100.1.
    BC080868 mRNA. Translation: AAH80868.1.
    CCDSiCCDS25922.1.
    PIRiS53724.
    RefSeqiNP_001139670.1. NM_001146198.1.
    NP_033411.3. NM_009385.3.
    UniGeneiMm.89972.

    Genome annotation databases

    EnsembliENSMUST00000001536; ENSMUSP00000001536; ENSMUSG00000001496.
    ENSMUST00000178477; ENSMUSP00000136103; ENSMUSG00000001496.
    GeneIDi21869.
    KEGGimmu:21869.
    UCSCiuc007npf.3. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U19755 Genomic DNA. Translation: AAA86100.1 .
    BC080868 mRNA. Translation: AAH80868.1 .
    CCDSi CCDS25922.1.
    PIRi S53724.
    RefSeqi NP_001139670.1. NM_001146198.1.
    NP_033411.3. NM_009385.3.
    UniGenei Mm.89972.

    3D structure databases

    ProteinModelPortali P50220.
    SMRi P50220. Positions 161-227.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204207. 12 interactions.
    STRINGi 10090.ENSMUSP00000001536.

    PTM databases

    PhosphoSitei P50220.

    Proteomic databases

    PaxDbi P50220.
    PRIDEi P50220.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000001536 ; ENSMUSP00000001536 ; ENSMUSG00000001496 .
    ENSMUST00000178477 ; ENSMUSP00000136103 ; ENSMUSG00000001496 .
    GeneIDi 21869.
    KEGGi mmu:21869.
    UCSCi uc007npf.3. mouse.

    Organism-specific databases

    CTDi 7080.
    MGIi MGI:108067. Nkx2-1.

    Phylogenomic databases

    eggNOGi NOG328614.
    GeneTreei ENSGT00720000108380.
    HOGENOMi HOG000231923.
    HOVERGENi HBG006689.
    InParanoidi P50220.
    KOi K09342.
    OMAi KSMAPLQ.
    OrthoDBi EOG769ZK0.
    PhylomeDBi P50220.
    TreeFami TF351204.

    Miscellaneous databases

    NextBioi 301368.
    PROi P50220.
    SOURCEi Search...

    Gene expression databases

    Bgeei P50220.
    CleanExi MM_NKX2-1.
    MM_TTF1.
    Genevestigatori P50220.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    InterProi IPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR020479. Homeobox_metazoa.
    IPR009057. Homeodomain-like.
    [Graphical view ]
    Pfami PF00046. Homeobox. 1 hit.
    [Graphical view ]
    PRINTSi PR00024. HOMEOBOX.
    SMARTi SM00389. HOX. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 1 hit.
    PROSITEi PS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete nucleotide sequence of the mouse thyroid-specific enhancer-binding protein (T/EBP) gene: extensive identity of the deduced amino acid sequence with the human protein."
      Oguchi H., Pan Y.-T., Kimura S.
      Biochim. Biophys. Acta 1261:304-306(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129/Sv.
      Tissue: Liver.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    3. "Thyroid transcription factor 1, a homeodomain containing transcription factor, contributes to regulating periodic oscillations in GnRH gene expression."
      Matagne V., Kim J.G., Ryu B.J., Hur M.K., Kim M.S., Kim K., Park B.S., Damante G., Smiley G., Lee B.J., Ojeda S.R.
      J. Neuroendocrinol. 24:916-929(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiNKX21_MOUSE
    AccessioniPrimary (citable) accession number: P50220
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 121 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3