Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P50220 (NKX21_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Homeobox protein Nkx-2.1
Alternative name(s):
Thyroid nuclear factor 1
Thyroid transcription factor 1
Short name=TTF-1
Gene names
Name:Nkx2-1
Synonyms:Nkx-2.1, Titf1, Ttf1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length372 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis.

Subunit structure

Interacts with WWTR1 By similarity.

Subcellular location

Nucleus.

Tissue specificity

Thyroid, lung and brain.

Post-translational modification

Phosphorylated on serine residues by STK3/MST2 By similarity.

Sequence similarities

Belongs to the NK-2 homeobox family.

Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   DomainHomeobox
   LigandDNA-binding
   Molecular functionActivator
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processClara cell differentiation

Inferred from genetic interaction PubMed 17164424. Source: MGI

Leydig cell differentiation

Inferred from mutant phenotype PubMed 12399445. Source: MGI

Type II pneumocyte differentiation

Inferred from genetic interaction PubMed 17164424. Source: MGI

anatomical structure formation involved in morphogenesis

Inferred from mutant phenotype PubMed 17522155. Source: MGI

axon guidance

Inferred from mutant phenotype PubMed 11830575. Source: MGI

brain development

Inferred from mutant phenotype PubMed 8557195. Source: MGI

cerebral cortex GABAergic interneuron differentiation

Inferred from mutant phenotype PubMed 15028753PubMed 18786356. Source: MGI

cerebral cortex cell migration

Inferred from mutant phenotype PubMed 18786357. Source: MGI

cerebral cortex neuron differentiation

Inferred from mutant phenotype PubMed 18339674. Source: MGI

developmental induction

Inferred from mutant phenotype PubMed 9806931. Source: MGI

endoderm development

Inferred from mutant phenotype PubMed 10208743PubMed 8557195. Source: MGI

epithelial tube branching involved in lung morphogenesis

Inferred from electronic annotation. Source: Ensembl

forebrain dorsal/ventral pattern formation

Inferred from mutant phenotype PubMed 10393115PubMed 11163262PubMed 11830575. Source: MGI

forebrain neuron differentiation

Inferred from mutant phenotype PubMed 11163262. Source: MGI

forebrain neuron fate commitment

Inferred from mutant phenotype PubMed 18339674. Source: MGI

globus pallidus development

Inferred from mutant phenotype PubMed 20181579. Source: MGI

hippocampus development

Inferred from mutant phenotype PubMed 11163262. Source: MGI

locomotory behavior

Inferred from mutant phenotype PubMed 11854318. Source: MGI

lung development

Inferred from mutant phenotype PubMed 10706142PubMed 12829717. Source: MGI

lung saccule development

Inferred from genetic interaction PubMed 17164424. Source: MGI

menarche

Inferred from mutant phenotype PubMed 17182767. Source: MGI

negative regulation of cell migration

Inferred from electronic annotation. Source: Ensembl

negative regulation of epithelial to mesenchymal transition

Inferred from electronic annotation. Source: Ensembl

negative regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 18786357. Source: MGI

negative regulation of transforming growth factor beta receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

neuron fate commitment

Inferred from mutant phenotype PubMed 18786356PubMed 20181579. Source: MGI

neuron migration

Inferred from mutant phenotype PubMed 12902388. Source: MGI

oligodendrocyte differentiation

Inferred from genetic interaction PubMed 11171336PubMed 11493571. Source: MGI

organ morphogenesis

Inferred from mutant phenotype PubMed 8557195. Source: MGI

pattern specification process

Inferred from genetic interaction PubMed 12930780. Source: MGI

phospholipid metabolic process

Inferred from genetic interaction PubMed 17164424. Source: MGI

pituitary gland development

Inferred from mutant phenotype PubMed 9806931. Source: MGI

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 11914369PubMed 14970209PubMed 16033766PubMed 17409236PubMed 18033672PubMed 18339674PubMed 18632661PubMed 7896788. Source: MGI

positive regulation of transcription, DNA-templated

Inferred from direct assay PubMed 15356023. Source: UniProtKB

regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 18632661. Source: MGI

regulation of transcription, DNA-templated

Inferred from direct assay PubMed 11733512. Source: MGI

response to hormone

Inferred from electronic annotation. Source: Ensembl

telencephalon cell migration

Inferred from mutant phenotype PubMed 18786357PubMed 20181579. Source: MGI

telencephalon development

Inferred from mutant phenotype PubMed 18786356. Source: MGI

thyroid gland development

Inferred from mutant phenotype PubMed 15581879PubMed 16601074. Source: MGI

transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 18033672PubMed 7896788. Source: GOC

   Cellular_componentnucleoplasm

Inferred from sequence or structural similarity. Source: UniProtKB

nucleus

Inferred from direct assay PubMed 11724907PubMed 12829717PubMed 15576401PubMed 16601074PubMed 16998587PubMed 17079460PubMed 17522155PubMed 19010321PubMed 20181579PubMed 7896788PubMed 8813084. Source: MGI

transcription factor complex

Inferred from direct assay PubMed 11733512PubMed 14970209PubMed 17164424. Source: MGI

   Molecular_functionRNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 18033672PubMed 7896788. Source: MGI

sequence-specific DNA binding

Inferred from direct assay PubMed 11914369PubMed 7896788. Source: MGI

sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 11733512PubMed 11914369PubMed 17164424PubMed 18786357. Source: MGI

transcription regulatory region DNA binding

Inferred from direct assay PubMed 18632661. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 372372Homeobox protein Nkx-2.1
PRO_0000049344

Regions

DNA binding161 – 22060Homeobox
Compositional bias234 – 24411Poly-Gly
Compositional bias247 – 2548Poly-Gln
Compositional bias295 – 30410Poly-Ala

Sequences

Sequence LengthMass (Da)Tools
P50220 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 377B5CE9B709412F

FASTA37238,570
        10         20         30         40         50         60 
MSMSPKHTTP FSVSDILSPL EESYKKVGME GGGLGAPLAA YRQGQAAPPA AAMQQHAVGH 

        70         80         90        100        110        120 
HGAVTAAYHM TAAGVPQLSH SAVGGYCNGN LGNMSELPPY QDTMRNSASG PGWYGANPDP 

       130        140        150        160        170        180 
RFPAISRFMG PASGMNMSGM GGLGSLGDVS KNMAPLPSAP RRKRRVLFSQ AQVYELERRF 

       190        200        210        220        230        240 
KQQKYLSAPE REHLASMIHL TPTQVKIWFQ NHRYKMKRQA KDKAAQQQLQ QDSGGGGGGG 

       250        260        270        280        290        300 
GGAGCPQQQQ AQQQSPRRVA VPVLVKDGKP CQAGAPAPGA ASLQSHAQQQ AQQQAQAAQA 

       310        320        330        340        350        360 
AAAAISVGSG GAGLGAHPGH QPGSAGQSPD LAHHAASPAG LQGQVSSLSH LNSSGSDYGA 

       370 
MSCSTLLYGR TW 

« Hide

References

« Hide 'large scale' references
[1]"The complete nucleotide sequence of the mouse thyroid-specific enhancer-binding protein (T/EBP) gene: extensive identity of the deduced amino acid sequence with the human protein."
Oguchi H., Pan Y.-T., Kimura S.
Biochim. Biophys. Acta 1261:304-306(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/Sv.
Tissue: Liver.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U19755 Genomic DNA. Translation: AAA86100.1.
BC080868 mRNA. Translation: AAH80868.1.
PIRS53724.
RefSeqNP_001139670.1. NM_001146198.1.
NP_033411.3. NM_009385.3.
UniGeneMm.89972.

3D structure databases

ProteinModelPortalP50220.
SMRP50220. Positions 161-227.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid204207. 12 interactions.
STRING10090.ENSMUSP00000001536.

PTM databases

PhosphoSiteP50220.

Proteomic databases

PaxDbP50220.
PRIDEP50220.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000001536; ENSMUSP00000001536; ENSMUSG00000001496.
ENSMUST00000178477; ENSMUSP00000136103; ENSMUSG00000001496.
GeneID21869.
KEGGmmu:21869.
UCSCuc007npf.3. mouse.

Organism-specific databases

CTD7080.
MGIMGI:108067. Nkx2-1.

Phylogenomic databases

eggNOGNOG328614.
GeneTreeENSGT00720000108380.
HOGENOMHOG000231923.
HOVERGENHBG006689.
InParanoidP50220.
KOK09342.
OMAQVSQAAM.
OrthoDBEOG769ZK0.
PhylomeDBP50220.
TreeFamTF351204.

Gene expression databases

BgeeP50220.
CleanExMM_NKX2-1.
MM_TTF1.
GenevestigatorP50220.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
[Graphical view]
PfamPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSPR00024. HOMEOBOX.
SMARTSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio301368.
PROP50220.
SOURCESearch...

Entry information

Entry nameNKX21_MOUSE
AccessionPrimary (citable) accession number: P50220
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot