P50220 (NKX21_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homeobox protein Nkx-2.1

Alternative name(s):
Thyroid nuclear factor 1
Thyroid transcription factor 1
Short name=TTF-1

Gene names

Name:	Nkx2-1
Synonyms:	Nkx-2.1, Titf1, Ttf1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis.
Subunit structure	Interacts with WWTR1 By similarity.
Subcellular location	Nucleus.
Tissue specificity	Thyroid, lung and brain.
Post-translational modification	Phosphorylated on serine residues by STK3/MST2 By similarity.
Sequence similarities	Belongs to the NK-2 homeobox family. Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Homeobox
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Clara cell differentiation Inferred from genetic interaction PubMed 17164424. Source: MGI Leydig cell differentiation Inferred from mutant phenotype PubMed 12399445. Source: MGI Type II pneumocyte differentiation Inferred from genetic interaction PubMed 17164424. Source: MGI anatomical structure formation involved in morphogenesis Inferred from mutant phenotype PubMed 17522155. Source: MGI axon guidance Inferred from mutant phenotype PubMed 11830575. Source: MGI cerebral cortex GABAergic interneuron differentiation Inferred from mutant phenotype PubMed 15028753PubMed 18786356. Source: MGI cerebral cortex cell migration Inferred from mutant phenotype PubMed 18786357. Source: MGI developmental induction Inferred from mutant phenotype PubMed 9806931. Source: MGI endoderm development Inferred from mutant phenotype PubMed 10208743PubMed 8557195. Source: MGI epithelial tube branching involved in lung morphogenesis Inferred from electronic annotation. Source: Compara forebrain dorsal/ventral pattern formation Inferred from mutant phenotype PubMed 10393115PubMed 11163262PubMed 11830575. Source: MGI forebrain neuron fate commitment Inferred from mutant phenotype PubMed 18339674. Source: MGI globus pallidus development Inferred from mutant phenotype PubMed 20181579. Source: MGI hippocampus development Inferred from mutant phenotype PubMed 11163262. Source: MGI locomotory behavior Inferred from mutant phenotype PubMed 11854318. Source: MGI lung saccule development Inferred from genetic interaction PubMed 17164424. Source: MGI menarche Inferred from mutant phenotype PubMed 17182767. Source: MGI negative regulation of cell migration Inferred from electronic annotation. Source: Compara negative regulation of epithelial to mesenchymal transition Inferred from electronic annotation. Source: Compara negative regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 18786357. Source: MGI negative regulation of transforming growth factor beta receptor signaling pathway Inferred from electronic annotation. Source: Compara neuron migration Inferred from mutant phenotype PubMed 12902388. Source: MGI oligodendrocyte differentiation Inferred from genetic interaction PubMed 11171336PubMed 11493571. Source: MGI organ morphogenesis Inferred from mutant phenotype PubMed 8557195. Source: MGI phospholipid metabolic process Inferred from genetic interaction PubMed 17164424. Source: MGI pituitary gland development Inferred from mutant phenotype PubMed 9806931. Source: MGI positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 11914369PubMed 14970209PubMed 16033766PubMed 17409236PubMed 18033672PubMed 18339674PubMed 18632661PubMed 7896788. Source: MGI response to hormone stimulus Inferred from electronic annotation. Source: Compara thyroid gland development Inferred from mutant phenotype PubMed 15581879PubMed 16601074. Source: MGI
Cellular_component	transcription factor complex Inferred from direct assay PubMed 11733512PubMed 14970209PubMed 17164424. Source: MGI
Molecular_function	RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Inferred from direct assay PubMed 18033672PubMed 7896788. Source: MGI sequence-specific DNA binding Inferred from direct assay PubMed 11914369PubMed 7896788. Source: MGI transcription regulatory region DNA binding Inferred from direct assay PubMed 18632661. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	Homeobox protein Nkx-2.1		PRO_0000049344
Regions
	DNA binding	161 – 220	60	Homeobox
	Compositional bias	234 – 244	11	Poly-Gly
	Compositional bias	247 – 254	8	Poly-Gln
	Compositional bias	295 – 304	10	Poly-Ala

Sequences

	Sequence		Length	Mass (Da)
	P50220 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 377B5CE9B709412F	FASTA	372	38,570
	10 20 30 40 50 60 MSMSPKHTTP FSVSDILSPL EESYKKVGME GGGLGAPLAA YRQGQAAPPA AAMQQHAVGH 70 80 90 100 110 120 HGAVTAAYHM TAAGVPQLSH SAVGGYCNGN LGNMSELPPY QDTMRNSASG PGWYGANPDP 130 140 150 160 170 180 RFPAISRFMG PASGMNMSGM GGLGSLGDVS KNMAPLPSAP RRKRRVLFSQ AQVYELERRF 190 200 210 220 230 240 KQQKYLSAPE REHLASMIHL TPTQVKIWFQ NHRYKMKRQA KDKAAQQQLQ QDSGGGGGGG 250 260 270 280 290 300 GGAGCPQQQQ AQQQSPRRVA VPVLVKDGKP CQAGAPAPGA ASLQSHAQQQ AQQQAQAAQA 310 320 330 340 350 360 AAAAISVGSG GAGLGAHPGH QPGSAGQSPD LAHHAASPAG LQGQVSSLSH LNSSGSDYGA 370 MSCSTLLYGR TW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete nucleotide sequence of the mouse thyroid-specific enhancer-binding protein (T/EBP) gene: extensive identity of the deduced amino acid sequence with the human protein." Oguchi H., Pan Y.-T., Kimura S. Biochim. Biophys. Acta 1261:304-306(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/Sv. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U19755 Genomic DNA. Translation: AAA86100.1. BC080868 mRNA. Translation: AAH80868.1.
IPI	IPI00116260.
PIR	S53724.
RefSeq	NP_001139670.1. NM_001146198.1. NP_033411.3. NM_009385.3.
UniGene	Mm.89972.
3D structure databases
ProteinModelPortal	P50220.
SMR	P50220. Positions 161-227.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000001536.
PTM databases
PhosphoSite	P50220.
Proteomic databases
PaxDb	P50220.
PRIDE	P50220.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000001536; ENSMUSP00000001536; ENSMUSG00000001496. ENSMUST00000178477; ENSMUSP00000136103; ENSMUSG00000001496.
GeneID	21869.
KEGG	mmu:21869.
UCSC	uc007npf.2. mouse.
Organism-specific databases
CTD	7080.
MGI	MGI:108067. Nkx2-1.
Phylogenomic databases
eggNOG	NOG328614.
GeneTree	ENSGT00700000104098.
HOGENOM	HOG000231923.
HOVERGEN	HBG006689.
InParanoid	P50220.
KO	K09342.
OMA	MRNSASA.
OrthoDB	EOG4X6CB3.
Gene expression databases
Bgee	P50220.
CleanEx	MM_NKX2-1. MM_TTF1.
Genevestigator	P50220.
GermOnline	ENSMUSG00000001496. Mus musculus.
Family and domain databases
Gene3D	1.10.10.60. 1 hit.
InterPro	IPR017970. Homeobox_CS. IPR020479. Homeobox_metazoa. IPR001356. Homeodomain. IPR009057. Homeodomain-like. [Graphical view]
Pfam	PF00046. Homeobox. 1 hit. [Graphical view]
PRINTS	PR00024. HOMEOBOX.
SMART	SM00389. HOX. 1 hit. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 1 hit.
PROSITE	PS00027. HOMEOBOX_1. 1 hit. PS50071. HOMEOBOX_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	301368.
SOURCE	Search...

Entry information

Entry name

NKX21_MOUSE

Accession

Primary (citable) accession number: P50220

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 109 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program