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Protein

Isocitrate dehydrogenase [NADP]

Gene
N/A
Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

May supply 2-oxoglutarate for amino acid biosynthesis and ammonia assimilation via the glutamine synthetase/glutamate synthase (GS/GOGAT) pathway.By similarity

Catalytic activityi

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg2+ or Mn2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei79SubstrateBy similarity1
Binding sitei84NADPBy similarity1
Binding sitei111SubstrateBy similarity1
Binding sitei134SubstrateBy similarity1
Sitei141Critical for catalysisBy similarity1
Sitei214Critical for catalysisBy similarity1
Metal bindingi254Magnesium or manganeseBy similarity1
Binding sitei262NADPBy similarity1
Metal bindingi277Magnesium or manganeseBy similarity1
Binding sitei330NADP; via amide nitrogen and carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi77 – 79NADPBy similarity3
Nucleotide bindingi312 – 317NADPBy similarity6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NADP] (EC:1.1.1.42)
Short name:
IDH
Alternative name(s):
IDP
NADP(+)-specific ICDH
Oxalosuccinate decarboxylase
OrganismiNicotiana tabacum (Common tobacco)
Taxonomic identifieri4097 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000835861 – 415Isocitrate dehydrogenase [NADP]Add BLAST415

Proteomic databases

PRIDEiP50218.

Interactioni

Subunit structurei

Heterodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP50218.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni96 – 102Substrate bindingBy similarity7

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
SMARTiSM01329. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50218-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTFDKIKVEN PIVEMDGDEM TRVIWKSIKD KLICPFLELD IKYFDLGLPH
60 70 80 90 100
RDATDDKVTV ESAEATQKYN VAIKCATITP DEARVKEFNL KSMWRSPNGT
110 120 130 140 150
IRNILNGTVF REPIMCKNIP RLVPGWTKPI CIGRHAFGDQ YRATDTVIQG
160 170 180 190 200
AGKLKLVFVP EGTDEKTEFE VYNFTGAGGV ALSMYNTDES VRSFAEASMN
210 220 230 240 250
MAYQKKWPLY LSTKNTILKK YDGRFKDIFQ EVYEANWKSK YEEAGIWYEH
260 270 280 290 300
RLIDDMAAYA LKSEGGYVWA CKNYDGDVQS DFLAQGFGSL GLMTSVLVCP
310 320 330 340 350
DGKTIEAEAA HGTVTRHYRV HQKGGETSTN SIASIFAWTR GLAHRATLDN
360 370 380 390 400
NERLLDFTEK LEAACIGAVE SGKMTKDLAL IIHGSKLSRD HYLNTEEFID
410
AVADELKARL LKAKA
Length:415
Mass (Da):46,729
Last modified:October 1, 1996 - v1
Checksum:iF9469AA113DB2CFC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77944 mRNA. Translation: CAA54912.1.
PIRiS65065.
RefSeqiNP_001312892.1. NM_001325963.1.
UniGeneiNta.7263.

Genome annotation databases

GeneIDi107815853.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77944 mRNA. Translation: CAA54912.1.
PIRiS65065.
RefSeqiNP_001312892.1. NM_001325963.1.
UniGeneiNta.7263.

3D structure databases

ProteinModelPortaliP50218.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP50218.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi107815853.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
SMARTiSM01329. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIDHC_TOBAC
AccessioniPrimary (citable) accession number: P50218
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 5, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.