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P50218 (IDHC_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Isocitrate dehydrogenase [NADP]

Short name=IDH
EC=1.1.1.42
Alternative name(s):
IDP
NADP(+)-specific ICDH
Oxalosuccinate decarboxylase
OrganismNicotiana tabacum (Common tobacco)
Taxonomic identifier4097 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Protein attributes

Sequence length415 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

May supply 2-oxoglutarate for amino acid biosynthesis and ammonia assimilation via the glutamine synthetase/glutamate synthase (GS/GOGAT) pathway By similarity.

Catalytic activity

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Subunit structure

Heterodimer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 415415Isocitrate dehydrogenase [NADP]
PRO_0000083586

Regions

Nucleotide binding77 – 793NADP By similarity
Nucleotide binding312 – 3176NADP By similarity
Region96 – 1027Substrate binding By similarity

Sites

Metal binding2541Magnesium or manganese By similarity
Metal binding2771Magnesium or manganese By similarity
Binding site791Substrate By similarity
Binding site841NADP By similarity
Binding site1111Substrate By similarity
Binding site1341Substrate By similarity
Binding site2621NADP By similarity
Binding site3301NADP; via amide nitrogen and carbonyl oxygen By similarity
Site1411Critical for catalysis By similarity
Site2141Critical for catalysis By similarity

Sequences

Sequence LengthMass (Da)Tools
P50218 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: F9469AA113DB2CFC

FASTA41546,729
        10         20         30         40         50         60 
MTFDKIKVEN PIVEMDGDEM TRVIWKSIKD KLICPFLELD IKYFDLGLPH RDATDDKVTV 

        70         80         90        100        110        120 
ESAEATQKYN VAIKCATITP DEARVKEFNL KSMWRSPNGT IRNILNGTVF REPIMCKNIP 

       130        140        150        160        170        180 
RLVPGWTKPI CIGRHAFGDQ YRATDTVIQG AGKLKLVFVP EGTDEKTEFE VYNFTGAGGV 

       190        200        210        220        230        240 
ALSMYNTDES VRSFAEASMN MAYQKKWPLY LSTKNTILKK YDGRFKDIFQ EVYEANWKSK 

       250        260        270        280        290        300 
YEEAGIWYEH RLIDDMAAYA LKSEGGYVWA CKNYDGDVQS DFLAQGFGSL GLMTSVLVCP 

       310        320        330        340        350        360 
DGKTIEAEAA HGTVTRHYRV HQKGGETSTN SIASIFAWTR GLAHRATLDN NERLLDFTEK 

       370        380        390        400        410 
LEAACIGAVE SGKMTKDLAL IIHGSKLSRD HYLNTEEFID AVADELKARL LKAKA 

« Hide

References

[1]"Identification of a tobacco cDNA encoding a cytosolic NADP-isocitrate dehydrogenase."
Galvez S., Hodges M., Decottignies P., Lancien M., Sangwan R., Dubois F., Lemarechal P., Cretin C., Gadal P.
Plant Mol. Biol. 30:307-320(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X77944 mRNA. Translation: CAA54912.1.
PIRS65065.

3D structure databases

ProteinModelPortalP50218.
SMRP50218. Positions 5-410.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP50218.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.718.10. 1 hit.
InterProIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERPTHR11822. PTHR11822. 1 hit.
PfamPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsTIGR00127. nadp_idh_euk. 1 hit.
PROSITEPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameIDHC_TOBAC
AccessionPrimary (citable) accession number: P50218
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: February 19, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families