Reviewed, UniProtKB/Swiss-Prot P50218 (IDHC_TOBAC)

Names and origin

Protein names	Recommended name:     Isocitrate dehydrogenase [NADP]       Short name=IDH     EC=1.1.1.42 Alternative name(s):     Oxalosuccinate decarboxylase     NADP(+)-specific ICDH     IDP
Organism	Nicotiana tabacum (Common tobacco)
Taxonomic identifier	4097 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	415 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May supply 2-oxoglutarate for amino acid biosynthesis and ammonia assimilation.
Catalytic activity	Isocitrate + NADP(+) = 2-oxoglutarate + CO(2) + NADPH. Oxalosuccinate + NADP(+) = 2-oxoglutarate + CO(2) + NADPH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Subunit structure	Heterodimer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	Magnesium Manganese Metal-binding NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW isocitrate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	isocitrate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 415	415	Isocitrate dehydrogenase [NADP]		PRO_0000083586
Regions
	Nucleotide binding	77 – 79	3	NADP By similarity
	Nucleotide binding	312 – 317	6	NADP By similarity
	Region	96 – 102	7	Substrate binding By similarity
Sites
	Metal binding	254	1	Magnesium or manganese By similarity
	Metal binding	277	1	Magnesium or manganese By similarity
	Binding site	79	1	Substrate By similarity
	Binding site	84	1	NADP By similarity
	Binding site	111	1	Substrate By similarity
	Binding site	134	1	Substrate By similarity
	Binding site	262	1	NADP By similarity
	Binding site	330	1	NADP; via amide nitrogen and carbonyl oxygen By similarity
	Site	141	1	Critical for catalysis By similarity
	Site	214	1	Critical for catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	P50218-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: F9469AA113DB2CFC	FASTA	415	46,729
	10 20 30 40 50 60 MTFDKIKVEN PIVEMDGDEM TRVIWKSIKD KLICPFLELD IKYFDLGLPH RDATDDKVTV 70 80 90 100 110 120 ESAEATQKYN VAIKCATITP DEARVKEFNL KSMWRSPNGT IRNILNGTVF REPIMCKNIP 130 140 150 160 170 180 RLVPGWTKPI CIGRHAFGDQ YRATDTVIQG AGKLKLVFVP EGTDEKTEFE VYNFTGAGGV 190 200 210 220 230 240 ALSMYNTDES VRSFAEASMN MAYQKKWPLY LSTKNTILKK YDGRFKDIFQ EVYEANWKSK 250 260 270 280 290 300 YEEAGIWYEH RLIDDMAAYA LKSEGGYVWA CKNYDGDVQS DFLAQGFGSL GLMTSVLVCP 310 320 330 340 350 360 DGKTIEAEAA HGTVTRHYRV HQKGGETSTN SIASIFAWTR GLAHRATLDN NERLLDFTEK 370 380 390 400 410 LEAACIGAVE SGKMTKDLAL IIHGSKLSRD HYLNTEEFID AVADELKARL LKAKA

References

[1]	"Identification of a tobacco cDNA encoding a cytosolic NADP-isocitrate dehydrogenase." Galvez S., Hodges M., Decottignies P., Lancien M., Sangwan R., Dubois F., Lemarechal P., Cretin C., Gadal P. Plant Mol. Biol. 30:307-320(1996) [PubMed: 8616254] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X77944 mRNA. Translation: CAA54912.1.
PIR	S65065.
3D structure databases
HSSP	HSSP built from PDB template 1LWD based on UniProtKB P33198.
ModBase	Search...
Family and domain databases
InterPro	IPR004790. IsoCit_DHase_NADP-dep_euk. IPR001804. IsoCit_IM_DHase. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11822. IDH_NADP_euk. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000108. IDH_NADP. 1 hit.
ProDom	PD328597. Nadp_idh_plant. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00127. nadp_idh_euk. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IDHC_TOBAC

Accession

Primary (citable) accession number: P50218

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 25, 2008
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)