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P50217

- IDHC_SOLTU

UniProt

P50217 - IDHC_SOLTU

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Protein

Isocitrate dehydrogenase [NADP]

Gene
ICDH-1
Organism
Solanum tuberosum (Potato)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

May supply 2-oxoglutarate for amino acid biosynthesis and ammonia assimilation via the glutamine synthetase/glutamate synthase (GS/GOGAT) pathway By similarity.

Catalytic activityi

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Cofactori

Binds 1 magnesium or manganese ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei79 – 791Substrate By similarity
Binding sitei84 – 841NADP By similarity
Binding sitei111 – 1111Substrate By similarity
Binding sitei134 – 1341Substrate By similarity
Sitei141 – 1411Critical for catalysis By similarity
Sitei214 – 2141Critical for catalysis By similarity
Metal bindingi254 – 2541Magnesium or manganese By similarity
Binding sitei262 – 2621NADP By similarity
Metal bindingi277 – 2771Magnesium or manganese By similarity
Binding sitei330 – 3301NADP; via amide nitrogen and carbonyl oxygen By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi77 – 793NADP By similarity
Nucleotide bindingi312 – 3176NADP By similarity

GO - Molecular functioni

  1. copper ion binding Source: EnsemblPlants/Gramene
  2. isocitrate dehydrogenase (NADP+) activity Source: UniProtKB-EC
  3. magnesium ion binding Source: InterPro
  4. NAD binding Source: InterPro

GO - Biological processi

  1. defense response to bacterium Source: EnsemblPlants/Gramene
  2. glyoxylate cycle Source: UniProtKB-KW
  3. isocitrate metabolic process Source: InterPro
  4. NADP metabolic process Source: EnsemblPlants/Gramene
  5. response to cadmium ion Source: EnsemblPlants/Gramene
  6. response to salt stress Source: EnsemblPlants/Gramene
  7. response to zinc ion Source: EnsemblPlants/Gramene
  8. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NADP] (EC:1.1.1.42)
Short name:
IDH
Alternative name(s):
IDP
NADP(+)-specific ICDH
Oxalosuccinate decarboxylase
Gene namesi
Name:ICDH-1
OrganismiSolanum tuberosum (Potato)
Taxonomic identifieri4113 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanum
ProteomesiUP000011115: Unplaced

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. apoplast Source: EnsemblPlants/Gramene
  2. chloroplast stroma Source: EnsemblPlants/Gramene
  3. plasma membrane Source: EnsemblPlants/Gramene
  4. plasmodesma Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 416416Isocitrate dehydrogenase [NADP]PRO_0000083584Add
BLAST

Proteomic databases

PRIDEiP50217.

Interactioni

Subunit structurei

Heterodimer By similarity.

Structurei

3D structure databases

ProteinModelPortaliP50217.
SMRiP50217. Positions 2-411.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni96 – 1027Substrate binding By similarity

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50217-1 [UniParc]FASTAAdd to Basket

« Hide

MAFQKITVQN PIVEMDGDEM TRVIWKSIKD KLILPFLELD IKYFSLGLPH    50
RDATDDKVTV ESAEATQKYN VAIKCATITP DEARVKEFNL KSMWRSPNGT 100
IRNILNGTVF REPIMCKNIP RLVPGWTKPI CIGRHAFGDQ YRATDTVIKG 150
AGKLKLVFVP EGSDEKTEFE VYNFTGAGGV ALSMYNTDES VRSFAEASMN 200
MAFQKKWPLY LSTKNTILKK YDGRFKDIFQ EVYEANWKSK YEEAGIWYEH 250
RLIDDMVAYA LKSEGGYVWA CKNYDGDVQS DFLAQGFGSL GLMTSVLVCP 300
DGKTIEAEAA HGTVTRHYRV HQKGGETSTN SIASIFAWTR GLAHRATLDN 350
NERLLDFTEK LEAACIGAVE SGKMTKDLAL IIIHGSKLSR EHYLNTEEFI 400
DAVADELKAR LLKAKA 416
Length:416
Mass (Da):46,792
Last modified:October 1, 1996 - v1
Checksum:iB9A9D6AD53AB090D
GO

Sequence cautioni

The sequence CAA53300.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X75638 mRNA. Translation: CAA53300.1. Different initiation.
PIRiS47013.
T07402.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X75638 mRNA. Translation: CAA53300.1 . Different initiation.
PIRi S47013.
T07402.

3D structure databases

ProteinModelPortali P50217.
SMRi P50217. Positions 2-411.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P50217.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.718.10. 1 hit.
InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view ]
PANTHERi PTHR11822. PTHR11822. 1 hit.
Pfami PF00180. Iso_dh. 1 hit.
[Graphical view ]
PIRSFi PIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsi TIGR00127. nadp_idh_euk. 1 hit.
PROSITEi PS00470. IDH_IMDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and expression analysis of the cytosolic NADP(+)-dependent isocitrate dehydrogenase from potato. Implications for nitrogen metabolism."
    Fieuw S., Mueller-Roeber B., Galvez S., Willmitzer L.
    Plant Physiol. 107:905-913(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Desiree.
    Tissue: Leaf.

Entry informationi

Entry nameiIDHC_SOLTU
AccessioniPrimary (citable) accession number: P50217
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 19, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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