Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P50217

- IDHC_SOLTU

UniProt

P50217 - IDHC_SOLTU

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Isocitrate dehydrogenase [NADP]

Gene

ICDH-1

Organism
Solanum tuberosum (Potato)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

May supply 2-oxoglutarate for amino acid biosynthesis and ammonia assimilation via the glutamine synthetase/glutamate synthase (GS/GOGAT) pathway.By similarity

Catalytic activityi

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg(2+) or Mn(2+) ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei79 – 791SubstrateBy similarity
Binding sitei84 – 841NADPBy similarity
Binding sitei111 – 1111SubstrateBy similarity
Binding sitei134 – 1341SubstrateBy similarity
Sitei141 – 1411Critical for catalysisBy similarity
Sitei214 – 2141Critical for catalysisBy similarity
Metal bindingi254 – 2541Magnesium or manganeseBy similarity
Binding sitei262 – 2621NADPBy similarity
Metal bindingi277 – 2771Magnesium or manganeseBy similarity
Binding sitei330 – 3301NADP; via amide nitrogen and carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi77 – 793NADPBy similarity
Nucleotide bindingi312 – 3176NADPBy similarity

GO - Molecular functioni

  1. isocitrate dehydrogenase (NADP+) activity Source: UniProtKB-EC
  2. magnesium ion binding Source: InterPro
  3. NAD binding Source: InterPro

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-KW
  2. isocitrate metabolic process Source: InterPro
  3. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NADP] (EC:1.1.1.42)
Short name:
IDH
Alternative name(s):
IDP
NADP(+)-specific ICDH
Oxalosuccinate decarboxylase
Gene namesi
Name:ICDH-1
OrganismiSolanum tuberosum (Potato)
Taxonomic identifieri4113 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanum
ProteomesiUP000011115: Unplaced

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 416416Isocitrate dehydrogenase [NADP]PRO_0000083584Add
BLAST

Proteomic databases

PRIDEiP50217.

Interactioni

Subunit structurei

Heterodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP50217.
SMRiP50217. Positions 2-411.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni96 – 1027Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

InParanoidiP50217.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50217-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAFQKITVQN PIVEMDGDEM TRVIWKSIKD KLILPFLELD IKYFSLGLPH
60 70 80 90 100
RDATDDKVTV ESAEATQKYN VAIKCATITP DEARVKEFNL KSMWRSPNGT
110 120 130 140 150
IRNILNGTVF REPIMCKNIP RLVPGWTKPI CIGRHAFGDQ YRATDTVIKG
160 170 180 190 200
AGKLKLVFVP EGSDEKTEFE VYNFTGAGGV ALSMYNTDES VRSFAEASMN
210 220 230 240 250
MAFQKKWPLY LSTKNTILKK YDGRFKDIFQ EVYEANWKSK YEEAGIWYEH
260 270 280 290 300
RLIDDMVAYA LKSEGGYVWA CKNYDGDVQS DFLAQGFGSL GLMTSVLVCP
310 320 330 340 350
DGKTIEAEAA HGTVTRHYRV HQKGGETSTN SIASIFAWTR GLAHRATLDN
360 370 380 390 400
NERLLDFTEK LEAACIGAVE SGKMTKDLAL IIIHGSKLSR EHYLNTEEFI
410
DAVADELKAR LLKAKA
Length:416
Mass (Da):46,792
Last modified:October 1, 1996 - v1
Checksum:iB9A9D6AD53AB090D
GO

Sequence cautioni

The sequence CAA53300.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75638 mRNA. Translation: CAA53300.1. Different initiation.
PIRiS47013.
T07402.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75638 mRNA. Translation: CAA53300.1 . Different initiation.
PIRi S47013.
T07402.

3D structure databases

ProteinModelPortali P50217.
SMRi P50217. Positions 2-411.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P50217.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

InParanoidi P50217.

Family and domain databases

Gene3Di 3.40.718.10. 1 hit.
InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view ]
PANTHERi PTHR11822. PTHR11822. 1 hit.
Pfami PF00180. Iso_dh. 1 hit.
[Graphical view ]
PIRSFi PIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsi TIGR00127. nadp_idh_euk. 1 hit.
PROSITEi PS00470. IDH_IMDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and expression analysis of the cytosolic NADP(+)-dependent isocitrate dehydrogenase from potato. Implications for nitrogen metabolism."
    Fieuw S., Mueller-Roeber B., Galvez S., Willmitzer L.
    Plant Physiol. 107:905-913(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Desiree.
    Tissue: Leaf.

Entry informationi

Entry nameiIDHC_SOLTU
AccessioniPrimary (citable) accession number: P50217
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 26, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3