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P50217

- IDHC_SOLTU

UniProt

P50217 - IDHC_SOLTU

Protein

Isocitrate dehydrogenase [NADP]

Gene

ICDH-1

Organism
Solanum tuberosum (Potato)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    May supply 2-oxoglutarate for amino acid biosynthesis and ammonia assimilation via the glutamine synthetase/glutamate synthase (GS/GOGAT) pathway.By similarity

    Catalytic activityi

    Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

    Cofactori

    Binds 1 magnesium or manganese ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei79 – 791SubstrateBy similarity
    Binding sitei84 – 841NADPBy similarity
    Binding sitei111 – 1111SubstrateBy similarity
    Binding sitei134 – 1341SubstrateBy similarity
    Sitei141 – 1411Critical for catalysisBy similarity
    Sitei214 – 2141Critical for catalysisBy similarity
    Metal bindingi254 – 2541Magnesium or manganeseBy similarity
    Binding sitei262 – 2621NADPBy similarity
    Metal bindingi277 – 2771Magnesium or manganeseBy similarity
    Binding sitei330 – 3301NADP; via amide nitrogen and carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi77 – 793NADPBy similarity
    Nucleotide bindingi312 – 3176NADPBy similarity

    GO - Molecular functioni

    1. copper ion binding Source: EnsemblPlants/Gramene
    2. isocitrate dehydrogenase (NADP+) activity Source: UniProtKB-EC
    3. magnesium ion binding Source: InterPro
    4. NAD binding Source: InterPro

    GO - Biological processi

    1. defense response to bacterium Source: EnsemblPlants/Gramene
    2. glyoxylate cycle Source: UniProtKB-KW
    3. isocitrate metabolic process Source: InterPro
    4. NADP metabolic process Source: EnsemblPlants/Gramene
    5. response to cadmium ion Source: EnsemblPlants/Gramene
    6. response to salt stress Source: EnsemblPlants/Gramene
    7. response to zinc ion Source: EnsemblPlants/Gramene
    8. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding, NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isocitrate dehydrogenase [NADP] (EC:1.1.1.42)
    Short name:
    IDH
    Alternative name(s):
    IDP
    NADP(+)-specific ICDH
    Oxalosuccinate decarboxylase
    Gene namesi
    Name:ICDH-1
    OrganismiSolanum tuberosum (Potato)
    Taxonomic identifieri4113 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanum
    ProteomesiUP000011115: Unplaced

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. apoplast Source: EnsemblPlants/Gramene
    2. chloroplast stroma Source: EnsemblPlants/Gramene
    3. plasma membrane Source: EnsemblPlants/Gramene
    4. plasmodesma Source: EnsemblPlants/Gramene

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 416416Isocitrate dehydrogenase [NADP]PRO_0000083584Add
    BLAST

    Proteomic databases

    PRIDEiP50217.

    Interactioni

    Subunit structurei

    Heterodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP50217.
    SMRiP50217. Positions 2-411.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni96 – 1027Substrate bindingBy similarity

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR004790. Isocitrate_DH_NADP.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view]
    PANTHERiPTHR11822. PTHR11822. 1 hit.
    PfamiPF00180. Iso_dh. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000108. IDH_NADP. 1 hit.
    TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
    PROSITEiPS00470. IDH_IMDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P50217-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAFQKITVQN PIVEMDGDEM TRVIWKSIKD KLILPFLELD IKYFSLGLPH    50
    RDATDDKVTV ESAEATQKYN VAIKCATITP DEARVKEFNL KSMWRSPNGT 100
    IRNILNGTVF REPIMCKNIP RLVPGWTKPI CIGRHAFGDQ YRATDTVIKG 150
    AGKLKLVFVP EGSDEKTEFE VYNFTGAGGV ALSMYNTDES VRSFAEASMN 200
    MAFQKKWPLY LSTKNTILKK YDGRFKDIFQ EVYEANWKSK YEEAGIWYEH 250
    RLIDDMVAYA LKSEGGYVWA CKNYDGDVQS DFLAQGFGSL GLMTSVLVCP 300
    DGKTIEAEAA HGTVTRHYRV HQKGGETSTN SIASIFAWTR GLAHRATLDN 350
    NERLLDFTEK LEAACIGAVE SGKMTKDLAL IIIHGSKLSR EHYLNTEEFI 400
    DAVADELKAR LLKAKA 416
    Length:416
    Mass (Da):46,792
    Last modified:October 1, 1996 - v1
    Checksum:iB9A9D6AD53AB090D
    GO

    Sequence cautioni

    The sequence CAA53300.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X75638 mRNA. Translation: CAA53300.1. Different initiation.
    PIRiS47013.
    T07402.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X75638 mRNA. Translation: CAA53300.1 . Different initiation.
    PIRi S47013.
    T07402.

    3D structure databases

    ProteinModelPortali P50217.
    SMRi P50217. Positions 2-411.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P50217.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR004790. Isocitrate_DH_NADP.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view ]
    PANTHERi PTHR11822. PTHR11822. 1 hit.
    Pfami PF00180. Iso_dh. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000108. IDH_NADP. 1 hit.
    TIGRFAMsi TIGR00127. nadp_idh_euk. 1 hit.
    PROSITEi PS00470. IDH_IMDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression analysis of the cytosolic NADP(+)-dependent isocitrate dehydrogenase from potato. Implications for nitrogen metabolism."
      Fieuw S., Mueller-Roeber B., Galvez S., Willmitzer L.
      Plant Physiol. 107:905-913(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Desiree.
      Tissue: Leaf.

    Entry informationi

    Entry nameiIDHC_SOLTU
    AccessioniPrimary (citable) accession number: P50217
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3