Reviewed, UniProtKB/Swiss-Prot P50215 (IDH_SPHYA)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase [NADP]
      Short name=IDH
    EC=1.1.1.42
Alternative name(s):
    Oxalosuccinate decarboxylase
    NADP(+)-specific ICDH
    IDP

Gene names

Name:	icd
Synonyms:	idhA

Organism

Sphingomonas yanoikuyae

Taxonomic identifier

13690 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Sphingobium

Protein attributes

Sequence length	406 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH. Oxalosuccinate + NADP+ = 2-oxoglutarate + CO2 + NADPH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Ligand	Magnesium Manganese Metal-binding NADP
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW isocitrate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro isocitrate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 406	406	Isocitrate dehydrogenase [NADP]		PRO_0000083567
Regions
	Nucleotide binding	75 – 77	3	NADP By similarity
	Nucleotide binding	308 – 313	6	NADP By similarity
	Region	94 – 100	7	Substrate binding By similarity
Sites
	Metal binding	250	1	Magnesium or manganese By similarity
	Metal binding	273	1	Magnesium or manganese By similarity
	Binding site	77	1	Substrate By similarity
	Binding site	82	1	NADP By similarity
	Binding site	109	1	Substrate By similarity
	Binding site	132	1	Substrate By similarity
	Binding site	258	1	NADP By similarity
	Binding site	326	1	NADP; via amide nitrogen and carbonyl oxygen By similarity
	Site	139	1	Critical for catalysis By similarity
	Site	210	1	Critical for catalysis By similarity
Amino acid modifications
	Modified residue	94	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P50215-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 093F7E18303A89E7	FASTA	406	45,483
	10 20 30 40 50 60 MAKIKVKNPV VEIDGDEMTR IIWEWIRERL ILPYLDVDLK YYDLSVEKRD ETSDQITIDA 70 80 90 100 110 120 ANAIKEYGVG VKCATITPDE ARVEEFGLKK MWKSPNGTIR NILGGVVFRE PIVIKNVPRL 130 140 150 160 170 180 VPGWTDPIVV GRHAFGDQYK ATDFKVPGAG TLTMKWVGTN GEELEYEVFE FPSAGVAMGM 190 200 210 220 230 240 YNLDESIRDF AKASFNYGLN RGWPVYLSTK NTILKAYDGR FKDLFQEVFD AEFADKFKAA 250 260 270 280 290 300 GIVYEHRLID DMVASALKWS GKFVWACKNY DGDVQSDTVA QGFGSLGLMT SVLLSPDGKT 310 320 330 340 350 360 VEAEAAHGTV TRHYRQHQQG KATSTNPIAS IFAWTQGLSF RGKFDDTPDV VKFAETLEQV 370 380 390 400 CIKTVEGGAM TKDLALLIGP DQAWMTTEQF FEAIRVNLEA EMAKWA

References

[1]	"Sequence and expression of an isocitrate dehydrogenase-encoding gene from a polycyclic aromatic hydrocarbon oxidizer, Sphingomonas yanoikuyae B1." Wang Y., Lau P.C.K. Gene 168:15-21(1996) [PubMed: 8626059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: B1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U37523 Genomic DNA. Translation: AAC43640.1.
PIR	JC4600.
3D structure databases
HSSP	HSSP built from PDB template 1LWD based on UniProtKB P33198.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.1.42. 189185.
Family and domain databases
InterPro	IPR019818. IsoCit/isopropylmalate_DH_CS. IPR001804. Isocitrate/isopropylmalate_DH. IPR004790. Isocitrate_DH_NADP-dep_euk. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11822. IDH_NADP_euk. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000108. IDH_NADP. 1 hit.
TIGRFAMs	TIGR00127. nadp_idh_euk. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IDH_SPHYA

Accession

Primary (citable) accession number: P50215

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)