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P50215

- IDH_SPHYA

UniProt

P50215 - IDH_SPHYA

Protein

Isocitrate dehydrogenase [NADP]

Gene

icd

Organism
Sphingobium yanoikuyae (Sphingomonas yanoikuyae)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

    Cofactori

    Binds 1 magnesium or manganese ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei77 – 771SubstrateBy similarity
    Binding sitei82 – 821NADPBy similarity
    Binding sitei109 – 1091SubstrateBy similarity
    Binding sitei132 – 1321SubstrateBy similarity
    Sitei139 – 1391Critical for catalysisBy similarity
    Sitei210 – 2101Critical for catalysisBy similarity
    Metal bindingi250 – 2501Magnesium or manganeseBy similarity
    Binding sitei258 – 2581NADPBy similarity
    Metal bindingi273 – 2731Magnesium or manganeseBy similarity
    Binding sitei326 – 3261NADP; via amide nitrogen and carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi75 – 773NADPBy similarity
    Nucleotide bindingi308 – 3136NADPBy similarity

    GO - Molecular functioni

    1. isocitrate dehydrogenase (NADP+) activity Source: UniProtKB-EC
    2. magnesium ion binding Source: InterPro
    3. NAD binding Source: InterPro

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-KW
    2. isocitrate metabolic process Source: InterPro
    3. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding, NADP

    Enzyme and pathway databases

    BioCyciRETL1328306-WGS:GSTH-2328-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isocitrate dehydrogenase [NADP] (EC:1.1.1.42)
    Short name:
    IDH
    Alternative name(s):
    IDP
    NADP(+)-specific ICDH
    Oxalosuccinate decarboxylase
    Gene namesi
    Name:icd
    Synonyms:idhA
    OrganismiSphingobium yanoikuyae (Sphingomonas yanoikuyae)
    Taxonomic identifieri13690 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeSphingobium

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 406406Isocitrate dehydrogenase [NADP]PRO_0000083567Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei94 – 941PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiP50215.

    Structurei

    3D structure databases

    ProteinModelPortaliP50215.
    SMRiP50215. Positions 3-404.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni94 – 1007Substrate bindingBy similarity

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR004790. Isocitrate_DH_NADP.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view]
    PANTHERiPTHR11822. PTHR11822. 1 hit.
    PfamiPF00180. Iso_dh. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000108. IDH_NADP. 1 hit.
    TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
    PROSITEiPS00470. IDH_IMDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P50215-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKIKVKNPV VEIDGDEMTR IIWEWIRERL ILPYLDVDLK YYDLSVEKRD    50
    ETSDQITIDA ANAIKEYGVG VKCATITPDE ARVEEFGLKK MWKSPNGTIR 100
    NILGGVVFRE PIVIKNVPRL VPGWTDPIVV GRHAFGDQYK ATDFKVPGAG 150
    TLTMKWVGTN GEELEYEVFE FPSAGVAMGM YNLDESIRDF AKASFNYGLN 200
    RGWPVYLSTK NTILKAYDGR FKDLFQEVFD AEFADKFKAA GIVYEHRLID 250
    DMVASALKWS GKFVWACKNY DGDVQSDTVA QGFGSLGLMT SVLLSPDGKT 300
    VEAEAAHGTV TRHYRQHQQG KATSTNPIAS IFAWTQGLSF RGKFDDTPDV 350
    VKFAETLEQV CIKTVEGGAM TKDLALLIGP DQAWMTTEQF FEAIRVNLEA 400
    EMAKWA 406
    Length:406
    Mass (Da):45,483
    Last modified:October 1, 1996 - v1
    Checksum:i093F7E18303A89E7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U37523 Genomic DNA. Translation: AAC43640.1.
    PIRiJC4600.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U37523 Genomic DNA. Translation: AAC43640.1 .
    PIRi JC4600.

    3D structure databases

    ProteinModelPortali P50215.
    SMRi P50215. Positions 3-404.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P50215.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BioCyci RETL1328306-WGS:GSTH-2328-MONOMER.

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR004790. Isocitrate_DH_NADP.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view ]
    PANTHERi PTHR11822. PTHR11822. 1 hit.
    Pfami PF00180. Iso_dh. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000108. IDH_NADP. 1 hit.
    TIGRFAMsi TIGR00127. nadp_idh_euk. 1 hit.
    PROSITEi PS00470. IDH_IMDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and expression of an isocitrate dehydrogenase-encoding gene from a polycyclic aromatic hydrocarbon oxidizer, Sphingomonas yanoikuyae B1."
      Wang Y., Lau P.C.K.
      Gene 168:15-21(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: DSM 6900 / JCM 10274 / B1.

    Entry informationi

    Entry nameiIDH_SPHYA
    AccessioniPrimary (citable) accession number: P50215
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3