ID   IDH_ANASP               Reviewed;         473 AA.
AC   P50214;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   16-JUN-2009, entry version 54.
DE   RecName: Full=Isocitrate dehydrogenase [NADP];
DE            Short=IDH;
DE            EC=1.1.1.42;
DE   AltName: Full=Oxalosuccinate decarboxylase;
DE   AltName: Full=NADP(+)-specific ICDH;
DE   AltName: Full=IDP;
GN   Name=icd; OrderedLocusNames=alr1827;
OS   Anabaena sp. (strain PCC 7120).
OC   Bacteria; Cyanobacteria; Nostocales; Nostocaceae; Nostoc.
OX   NCBI_TaxID=103690;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=94222853; PubMed=8169222;
RA   Muro-Pastor M.I., Florencio F.J.;
RT   "NADP(+)-isocitrate dehydrogenase from the cyanobacterium Anabaena sp.
RT   strain PCC 7120: purification and characterization of the enzyme and
RT   cloning, sequencing, and disruption of the icd gene.";
RL   J. Bacteriol. 176:2718-2726(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   MEDLINE=21595285; PubMed=11759840; DOI=10.1093/dnares/8.5.205;
RA   Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S.,
RA   Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T.,
RA   Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A.,
RA   Nakazaki N., Shimpo S., Sugimoto M., Takazawa M., Yamada M.,
RA   Yasuda M., Tabata S.;
RT   "Complete genomic sequence of the filamentous nitrogen-fixing
RT   cyanobacterium Anabaena sp. strain PCC 7120.";
RL   DNA Res. 8:205-213(2001).
CC   -!- CATALYTIC ACTIVITY: Isocitrate + NADP(+) = 2-oxoglutarate + CO(2)
CC       + NADPH.
CC   -!- CATALYTIC ACTIVITY: Oxalosuccinate + NADP(+) = 2-oxoglutarate +
CC       CO(2) + NADPH.
CC   -!- COFACTOR: Binds 1 magnesium or manganese ion per subunit (By
CC       similarity).
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SIMILARITY: Belongs to the isocitrate and isopropylmalate
CC       dehydrogenases family.
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DR   EMBL; X77654; CAA54734.1; -; Genomic_DNA.
DR   EMBL; BA000019; BAB73526.1; -; Genomic_DNA.
DR   PIR; A55591; A55591.
DR   PIR; AE2034; AE2034.
DR   RefSeq; NP_485867.1; -.
DR   HSSP; P08200; 1PB1.
DR   GeneID; 1105419; -.
DR   GenomeReviews; BA000019_GR; alr1827.
DR   KEGG; ana:alr1827; -.
DR   NMPDR; fig|103690.1.peg.2134; -.
DR   HOGENOM; P50214; -.
DR   OMA; P50214; RDWGYEL.
DR   BioCyc; NSP103690:ALR1827-MON; -.
DR   GO; GO:0004450; F:isocitrate dehydrogenase (NADP+) activity; IEA:EC.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0030145; F:manganese ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0051287; F:NAD or NADH binding; IEA:InterPro.
DR   GO; GO:0006097; P:glyoxylate cycle; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR   InterPro; IPR019818; IsoCit/isopropylmalate_DH_CS.
DR   InterPro; IPR001804; Isocitrate/isopropylmalate_DH.
DR   InterPro; IPR004439; Isocitrate_DH_NADP_prok.
DR   Gene3D; G3DSA:3.40.718.10; IDH_IMDH; 1.
DR   PANTHER; PTHR11835; IDH_IMDH_dimeric; 1.
DR   PANTHER; PTHR11835:SF1; NADP_IDH_prok; 1.
DR   Pfam; PF00180; Iso_dh; 1.
DR   TIGRFAMs; TIGR00183; prok_nadp_idh; 1.
DR   PROSITE; PS00470; IDH_IMDH; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Glyoxylate bypass; Magnesium; Manganese;
KW   Metal-binding; NADP; Oxidoreductase; Phosphoprotein;
KW   Tricarboxylic acid cycle.
FT   CHAIN         1    473       Isocitrate dehydrogenase [NADP].
FT                                /FTId=PRO_0000083547.
FT   NP_BIND     394    400       NADP (By similarity).
FT   METAL       362    362       Magnesium or manganese (By similarity).
FT   BINDING     104    104       NADP (By similarity).
FT   BINDING     113    113       Substrate (By similarity).
FT   BINDING     115    115       Substrate (By similarity).
FT   BINDING     119    119       Substrate (By similarity).
FT   BINDING     129    129       Substrate (By similarity).
FT   BINDING     153    153       Substrate (By similarity).
FT   BINDING     407    407       NADP; via amide nitrogen and carbonyl
FT                                oxygen (By similarity).
FT   BINDING     446    446       NADP (By similarity).
FT   BINDING     450    450       NADP (By similarity).
FT   SITE        160    160       Critical for catalysis (By similarity).
FT   SITE        237    237       Critical for catalysis (By similarity).
FT   MOD_RES     113    113       Phosphoserine (By similarity).
SQ   SEQUENCE   473 AA;  52228 MW;  D30941B992A38AAB CRC64;
     MYNKITPPTT GEKITFKNGE PVVPDNPIIP FIRGDGTGID IWPATEKVLD AAVAKAYQGK
     RKISWFKVYA GDEACDLYGT YQYLPEDTLT AIREYGVAIK GPLTTPVGGG IRSLNVALRQ
     IFDLYACVRP CRYYAGTPSP HKNPEKLDVI VYRENTEDIY LGIEWKQGSE IGDRLISILN
     KELIPATPEH GKKQIPLDSG IGIKPISKTG SQRLVRRAIK HALTLPKDKQ QVTLVHKGNI
     MKYTEGAFRD WGYELATSEF RQETVTERES WILSNKEKNP NISLEDNARQ IDPGFDALTP
     EKKAQIVKEV ETVLNSIWES HGNGKWKEKV LVNDRIADSI FQQIQTRPDE YSILATMNLN
     GDYLSDAAAA IVGGLGMGPG ANIGDSCAVF EATHGTAPKH AGLDRINPGS VILSGVMMLE
     YMGWQEAADL IKKGLSDAIA NSQVTYDLAR LLEPPVEPLK CSEFADAIIK HFG
//