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P50214 (IDH_NOSS1) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Isocitrate dehydrogenase [NADP]

Short name=IDH
EC=1.1.1.42
Alternative name(s):
IDP
NADP(+)-specific ICDH
Oxalosuccinate decarboxylase
Gene names
Name:icd
Ordered Locus Names:alr1827
OrganismNostoc sp. (strain PCC 7120 / UTEX 2576) [Complete proteome] [HAMAP]
Taxonomic identifier103690 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaNostocalesNostocaceaeNostoc

Protein attributes

Sequence length473 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 473473Isocitrate dehydrogenase [NADP]
PRO_0000083547

Regions

Nucleotide binding394 – 4007NADP By similarity

Sites

Metal binding3621Magnesium or manganese By similarity
Binding site1041NADP By similarity
Binding site1131Substrate By similarity
Binding site1151Substrate By similarity
Binding site1191Substrate By similarity
Binding site1291Substrate By similarity
Binding site1531Substrate By similarity
Binding site4071NADP; via amide nitrogen and carbonyl oxygen By similarity
Binding site4461NADP By similarity
Binding site4501NADP By similarity
Site1601Critical for catalysis By similarity
Site2371Critical for catalysis By similarity

Amino acid modifications

Modified residue1131Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
P50214 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: D30941B992A38AAB

FASTA47352,228
        10         20         30         40         50         60 
MYNKITPPTT GEKITFKNGE PVVPDNPIIP FIRGDGTGID IWPATEKVLD AAVAKAYQGK 

        70         80         90        100        110        120 
RKISWFKVYA GDEACDLYGT YQYLPEDTLT AIREYGVAIK GPLTTPVGGG IRSLNVALRQ 

       130        140        150        160        170        180 
IFDLYACVRP CRYYAGTPSP HKNPEKLDVI VYRENTEDIY LGIEWKQGSE IGDRLISILN 

       190        200        210        220        230        240 
KELIPATPEH GKKQIPLDSG IGIKPISKTG SQRLVRRAIK HALTLPKDKQ QVTLVHKGNI 

       250        260        270        280        290        300 
MKYTEGAFRD WGYELATSEF RQETVTERES WILSNKEKNP NISLEDNARQ IDPGFDALTP 

       310        320        330        340        350        360 
EKKAQIVKEV ETVLNSIWES HGNGKWKEKV LVNDRIADSI FQQIQTRPDE YSILATMNLN 

       370        380        390        400        410        420 
GDYLSDAAAA IVGGLGMGPG ANIGDSCAVF EATHGTAPKH AGLDRINPGS VILSGVMMLE 

       430        440        450        460        470 
YMGWQEAADL IKKGLSDAIA NSQVTYDLAR LLEPPVEPLK CSEFADAIIK HFG 

« Hide

References

« Hide 'large scale' references
[1]"NADP(+)-isocitrate dehydrogenase from the cyanobacterium Anabaena sp. strain PCC 7120: purification and characterization of the enzyme and cloning, sequencing, and disruption of the icd gene."
Muro-Pastor M.I., Florencio F.J.
J. Bacteriol. 176:2718-2726(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120."
Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. expand/collapse author list , Takazawa M., Yamada M., Yasuda M., Tabata S.
DNA Res. 8:205-213(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 7120 / UTEX 2576.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X77654 Genomic DNA. Translation: CAA54734.1.
BA000019 Genomic DNA. Translation: BAB73526.1.
PIRA55591.
AE2034.
RefSeqNP_485867.1. NC_003272.1.

3D structure databases

ProteinModelPortalP50214.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING103690.alr1827.

Proteomic databases

PRIDEP50214.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB73526; BAB73526; BAB73526.
GeneID1105419.
KEGGana:alr1827.
PATRIC22773725. VBINosSp37423_2340.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0538.
HOGENOMHOG000021113.
KOK00031.
OMAAITIFRE.
OrthoDBEOG6SNDTP.

Family and domain databases

Gene3D3.40.718.10. 2 hits.
InterProIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR004439. Isocitrate_DH_NADP_dimer_prok.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERPTHR11835. PTHR11835. 1 hit.
PfamPF00180. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsTIGR00183. prok_nadp_idh. 1 hit.
PROSITEPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameIDH_NOSS1
AccessionPrimary (citable) accession number: P50214
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 14, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families