Reviewed, UniProtKB/Swiss-Prot P50214 (IDH_ANASP)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase [NADP]
      Short name=IDH
    EC=1.1.1.42
Alternative name(s):
    Oxalosuccinate decarboxylase
    NADP(+)-specific ICDH
    IDP

Gene names

Name:	icd
Ordered Locus Names:	alr1827

Organism

Anabaena sp. (strain PCC 7120) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	473 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH. Oxalosuccinate + NADP+ = 2-oxoglutarate + CO2 + NADPH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Ligand	Magnesium Manganese Metal-binding NADP
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro isocitrate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 473	473	Isocitrate dehydrogenase [NADP]		PRO_0000083547
Regions
	Nucleotide binding	394 – 400	7	NADP By similarity
Sites
	Metal binding	362	1	Magnesium or manganese By similarity
	Binding site	104	1	NADP By similarity
	Binding site	113	1	Substrate By similarity
	Binding site	115	1	Substrate By similarity
	Binding site	119	1	Substrate By similarity
	Binding site	129	1	Substrate By similarity
	Binding site	153	1	Substrate By similarity
	Binding site	407	1	NADP; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	446	1	NADP By similarity
	Binding site	450	1	NADP By similarity
	Site	160	1	Critical for catalysis By similarity
	Site	237	1	Critical for catalysis By similarity
Amino acid modifications
	Modified residue	113	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P50214-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: D30941B992A38AAB	FASTA	473	52,228
	10 20 30 40 50 60 MYNKITPPTT GEKITFKNGE PVVPDNPIIP FIRGDGTGID IWPATEKVLD AAVAKAYQGK 70 80 90 100 110 120 RKISWFKVYA GDEACDLYGT YQYLPEDTLT AIREYGVAIK GPLTTPVGGG IRSLNVALRQ 130 140 150 160 170 180 IFDLYACVRP CRYYAGTPSP HKNPEKLDVI VYRENTEDIY LGIEWKQGSE IGDRLISILN 190 200 210 220 230 240 KELIPATPEH GKKQIPLDSG IGIKPISKTG SQRLVRRAIK HALTLPKDKQ QVTLVHKGNI 250 260 270 280 290 300 MKYTEGAFRD WGYELATSEF RQETVTERES WILSNKEKNP NISLEDNARQ IDPGFDALTP 310 320 330 340 350 360 EKKAQIVKEV ETVLNSIWES HGNGKWKEKV LVNDRIADSI FQQIQTRPDE YSILATMNLN 370 380 390 400 410 420 GDYLSDAAAA IVGGLGMGPG ANIGDSCAVF EATHGTAPKH AGLDRINPGS VILSGVMMLE 430 440 450 460 470 YMGWQEAADL IKKGLSDAIA NSQVTYDLAR LLEPPVEPLK CSEFADAIIK HFG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"NADP(+)-isocitrate dehydrogenase from the cyanobacterium Anabaena sp. strain PCC 7120: purification and characterization of the enzyme and cloning, sequencing, and disruption of the icd gene." Muro-Pastor M.I., Florencio F.J. J. Bacteriol. 176:2718-2726(1994) [PubMed: 8169222] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X77654 Genomic DNA. Translation: CAA54734.1. BA000019 Genomic DNA. Translation: BAB73526.1.
PIR	A55591. AE2034.
RefSeq	NP_485867.1.
3D structure databases
HSSP	HSSP built from PDB template 1PB1 based on UniProtKB P08200.
ModBase	Search...
Genome annotation databases
GeneID	1105419.
GenomeReviews	Gene locus alr1827 in contig BA000019_GR.
KEGG	ana:alr1827.
NMPDR	fig|103690.1.peg.2134.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P50214.
OMA	P50214. RDWGYEL.
Enzyme and pathway databases
BioCyc	NSP103690:ALR1827-MON.
Family and domain databases
InterPro	IPR019818. IsoCit/isopropylmalate_DH_CS. IPR001804. Isocitrate/isopropylmalate_DH. IPR004439. Isocitrate_DH_NADP_prok. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit. PTHR11835:SF1. NADP_IDH_prok. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR00183. prok_nadp_idh. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IDH_ANASP

Accession

Primary (citable) accession number: P50214

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)