Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P50213

- IDH3A_HUMAN

UniProt

P50213 - IDH3A_HUMAN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial

Gene

IDH3A

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg(2+) or Mn(2+) ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei115 – 1151SubstrateBy similarity
Binding sitei125 – 1251SubstrateBy similarity
Binding sitei146 – 1461SubstrateBy similarity
Sitei153 – 1531Critical for catalysisBy similarity
Sitei200 – 2001Critical for catalysisBy similarity
Metal bindingi233 – 2331Magnesium or manganeseBy similarity
Binding sitei233 – 2331SubstrateBy similarity
Metal bindingi257 – 2571Magnesium or manganeseBy similarity
Metal bindingi261 – 2611Magnesium or manganeseBy similarity

GO - Molecular functioni

  1. isocitrate dehydrogenase (NAD+) activity Source: UniProtKB-EC
  2. magnesium ion binding Source: InterPro
  3. NAD binding Source: InterPro

GO - Biological processi

  1. carbohydrate metabolic process Source: ProtInc
  2. cellular metabolic process Source: Reactome
  3. small molecule metabolic process Source: Reactome
  4. tricarboxylic acid cycle Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NAD

Enzyme and pathway databases

BioCyciMetaCyc:ENSG00000166411-MONOMER.
ReactomeiREACT_1785. Citric acid cycle (TCA cycle).
SABIO-RKP50213.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial (EC:1.1.1.41)
Alternative name(s):
Isocitric dehydrogenase subunit alpha
NAD(+)-specific ICDH subunit alpha
Gene namesi
Name:IDH3A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 15

Organism-specific databases

HGNCiHGNC:5384. IDH3A.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial matrix Source: Reactome
  2. mitochondrion Source: HPA
  3. nucleus Source: UniProt
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA29632.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2727MitochondrionBy similarityAdd
BLAST
Chaini28 – 366339Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrialPRO_0000014436Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei77 – 771N6-succinyllysineBy similarity
Modified residuei223 – 2231N6-acetyllysineBy similarity
Modified residuei343 – 3431N6-acetyllysine; alternate1 Publication
Modified residuei343 – 3431N6-succinyllysine; alternateBy similarity
Modified residuei350 – 3501N6-succinyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP50213.
PaxDbiP50213.
PRIDEiP50213.

2D gel databases

OGPiP50213.
REPRODUCTION-2DPAGEIPI00030702.

PTM databases

PhosphoSiteiP50213.

Expressioni

Gene expression databases

BgeeiP50213.
CleanExiHS_IDH3A.
ExpressionAtlasiP50213. baseline and differential.
GenevestigatoriP50213.

Organism-specific databases

HPAiHPA041465.

Interactioni

Subunit structurei

Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1.By similarity

Protein-protein interaction databases

BioGridi109645. 22 interactions.
IntActiP50213. 9 interactions.
MINTiMINT-1148360.
STRINGi9606.ENSP00000299518.

Structurei

3D structure databases

ProteinModelPortaliP50213.
SMRiP50213. Positions 4-362.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0473.
GeneTreeiENSGT00550000074918.
HOGENOMiHOG000021113.
HOVERGENiHBG052080.
InParanoidiP50213.
KOiK00030.
OMAiTPIAWEP.
OrthoDBiEOG75B85R.
PhylomeDBiP50213.
TreeFamiTF105692.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR004434. Isocitrate_DH_NAD.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11835. PTHR11835. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00175. mito_nad_idh. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P50213-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGPAWISKV SRLLGAFHNP KQVTRGFTGG VQTVTLIPGD GIGPEISAAV
60 70 80 90 100
MKIFDAAKAP IQWEERNVTA IQGPGGKWMI PSEAKESMDK NKMGLKGPLK
110 120 130 140 150
TPIAAGHPSM NLLLRKTFDL YANVRPCVSI EGYKTPYTDV NIVTIRENTE
160 170 180 190 200
GEYSGIEHVI VDGVVQSIKL ITEGASKRIA EFAFEYARNN HRSNVTAVHK
210 220 230 240 250
ANIMRMSDGL FLQKCREVAE SCKDIKFNEM YLDTVCLNMV QDPSQFDVLV
260 270 280 290 300
MPNLYGDILS DLCAGLIGGL GVTPSGNIGA NGVAIFESVH GTAPDIAGKD
310 320 330 340 350
MANPTALLLS AVMMLRHMGL FDHAARIEAA CFATIKDGKS LTKDLGGNAK
360
CSDFTEEICR RVKDLD
Length:366
Mass (Da):39,592
Last modified:October 1, 1996 - v1
Checksum:i695F6A34F97430CF
GO
Isoform 2 (identifier: P50213-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-78: Missing.

Note: No experimental confirmation available.

Show »
Length:288
Mass (Da):31,381
Checksum:iBF8327BD4AA7D3EC
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 7878Missing in isoform 2. 1 PublicationVSP_014516Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07681 mRNA. Translation: AAA85639.1.
AL442090 mRNA. Translation: CAC09449.1.
CH471136 Genomic DNA. Translation: EAW99181.1.
CH471136 Genomic DNA. Translation: EAW99182.1.
BC021967 mRNA. Translation: AAH21967.1.
CCDSiCCDS10297.1. [P50213-1]
PIRiS55282.
RefSeqiNP_005521.1. NM_005530.2. [P50213-1]
UniGeneiHs.591110.

Genome annotation databases

EnsembliENST00000299518; ENSP00000299518; ENSG00000166411. [P50213-1]
GeneIDi3419.
KEGGihsa:3419.
UCSCiuc002bdd.3. human. [P50213-1]

Polymorphism databases

DMDMi1708399.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07681 mRNA. Translation: AAA85639.1 .
AL442090 mRNA. Translation: CAC09449.1 .
CH471136 Genomic DNA. Translation: EAW99181.1 .
CH471136 Genomic DNA. Translation: EAW99182.1 .
BC021967 mRNA. Translation: AAH21967.1 .
CCDSi CCDS10297.1. [P50213-1 ]
PIRi S55282.
RefSeqi NP_005521.1. NM_005530.2. [P50213-1 ]
UniGenei Hs.591110.

3D structure databases

ProteinModelPortali P50213.
SMRi P50213. Positions 4-362.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 109645. 22 interactions.
IntActi P50213. 9 interactions.
MINTi MINT-1148360.
STRINGi 9606.ENSP00000299518.

PTM databases

PhosphoSitei P50213.

Polymorphism databases

DMDMi 1708399.

2D gel databases

OGPi P50213.
REPRODUCTION-2DPAGE IPI00030702.

Proteomic databases

MaxQBi P50213.
PaxDbi P50213.
PRIDEi P50213.

Protocols and materials databases

DNASUi 3419.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000299518 ; ENSP00000299518 ; ENSG00000166411 . [P50213-1 ]
GeneIDi 3419.
KEGGi hsa:3419.
UCSCi uc002bdd.3. human. [P50213-1 ]

Organism-specific databases

CTDi 3419.
GeneCardsi GC15P078423.
HGNCi HGNC:5384. IDH3A.
HPAi HPA041465.
MIMi 601149. gene.
neXtProti NX_P50213.
PharmGKBi PA29632.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG0473.
GeneTreei ENSGT00550000074918.
HOGENOMi HOG000021113.
HOVERGENi HBG052080.
InParanoidi P50213.
KOi K00030.
OMAi TPIAWEP.
OrthoDBi EOG75B85R.
PhylomeDBi P50213.
TreeFami TF105692.

Enzyme and pathway databases

BioCyci MetaCyc:ENSG00000166411-MONOMER.
Reactomei REACT_1785. Citric acid cycle (TCA cycle).
SABIO-RK P50213.

Miscellaneous databases

ChiTaRSi IDH3A. human.
GeneWikii IDH3A.
GenomeRNAii 3419.
NextBioi 13478.
PROi P50213.
SOURCEi Search...

Gene expression databases

Bgeei P50213.
CleanExi HS_IDH3A.
ExpressionAtlasi P50213. baseline and differential.
Genevestigatori P50213.

Family and domain databases

Gene3Di 3.40.718.10. 1 hit.
InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR004434. Isocitrate_DH_NAD.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view ]
PANTHERi PTHR11835. PTHR11835. 1 hit.
Pfami PF00180. Iso_dh. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00175. mito_nad_idh. 1 hit.
PROSITEi PS00470. IDH_IMDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of a cDNA clone for human NAD(+)-specific isocitrate dehydrogenase alpha-subunit and structural comparison with its isoenzymes from different species."
    Kim Y.O., Oh I.U., Park H.S., Jeng J., Song B.J., Huh T.L.
    Biochem. J. 308:63-68(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Heart.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Brain cortex.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Lymph.
  5. Lubec G., Vishwanath V., Chen W.-Q., Sun Y.
    Submitted (DEC-2008) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 135-146; 179-188 AND 300-316, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Brain, Cajal-Retzius cell and Fetal brain cortex.
  6. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
    Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
    Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-343, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiIDH3A_HUMAN
AccessioniPrimary (citable) accession number: P50213
Secondary accession number(s): D3DW83, Q9H3X0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 26, 2014
This is version 149 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3