ID   BPHB_PSES1              Reviewed;         276 AA.
AC   P50206;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   16-JUN-2009, entry version 47.
DE   RecName: Full=Cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase;
DE            EC=1.3.1.56;
DE   AltName: Full=Biphenyl-2,3-dihydro-2,3-diol dehydrogenase;
DE   AltName: Full=Biphenyl-cis-diol dehydrogenase;
DE   AltName: Full=2,3-dihydro-2,3-dihydroxybiphenyl dehydrogenase;
DE   AltName: Full=2,3-dihydroxy-4-phenylhexa-4,6-diene dehydrogenase;
GN   Name=bphB;
OS   Pseudomonas sp. (strain KKS102).
OC   Bacteria; Proteobacteria.
OX   NCBI_TaxID=307;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=94324977; PubMed=8048958; DOI=10.1006/bbrc.1994.2008;
RA   Fukuda M., Yasukochi Y., Kikuchi Y., Nagata Y., Kimbara K.,
RA   Horiuchi H., Takagi M., Yano K.;
RT   "Identification of the bphA and bphB genes of Pseudomonas sp. strains
RT   KKS102 involved in degradation of biphenyl and polychlorinated
RT   biphenyls.";
RL   Biochem. Biophys. Res. Commun. 202:850-856(1994).
CC   -!- CATALYTIC ACTIVITY: Cis-3-phenylcyclohexa-3,5-diene-1,2-diol +
CC       NAD(+) = biphenyl-2,3-diol + NADH.
CC   -!- PATHWAY: Xenobiotic degradation; biphenyl degradation; 2-hydroxy-
CC       2,4-pentadienoic acid and benzoic acid from biphenyl: step 2/4.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases
CC       (SDR) family.
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DR   EMBL; D17319; BAA04140.1; -; Genomic_DNA.
DR   PIR; JC2441; JC2441.
DR   HSSP; P47227; 1BDB.
DR   SMR; P50206; 1-275.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0018509; F:cis-2,3-dihydrobiphenyl-2,3-diol dehydrogen...; IEA:EC.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR017711; BphB_TodD.
DR   InterPro; IPR002198; DH_sc/Rdtase_SDR.
DR   InterPro; IPR002347; Glc/ribitol_DH.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR19410; ADH_short_C2; 1.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   TIGRFAMs; TIGR03325; BphB_TodD; 1.
DR   PROSITE; PS00061; ADH_SHORT; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; NAD; Oxidoreductase.
FT   CHAIN         1    276       Cis-2,3-dihydrobiphenyl-2,3-diol
FT                                dehydrogenase.
FT                                /FTId=PRO_0000054534.
FT   NP_BIND       9     33       NAD (By similarity).
FT   ACT_SITE    155    155       Proton acceptor (By similarity).
FT   BINDING     142    142       Substrate (By similarity).
SQ   SEQUENCE   276 AA;  28933 MW;  3C000F5EA72F44D1 CRC64;
     MQLNNEVALV TGGGSGLGRA IVDRFVAEGA RVAVLDKSAA RLQELQAAHG AKVLGIEGDV
     RVLADHQKAA RECVAAFGKI DCLIPNAGIW DYSMPLVDIP DDRIDAAFDE VFHINVKGYL
     LAVKACLPAL VQSRGSVVFT ISNAGFYPNG GGPLYTGAKH AVVGMVRELA YELAPHVRVN
     GVAPGGMSTD LRGPASLGMA NQAISSVPLG EMLTSVLPVG RMPVRAEYTG AYVFFATRGD
     TFPTTGALLN HDGGMGVRGF FEATGGKDLP QKLRLS
//