P50206 (BPHB_PSES1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase
EC=1.3.1.56

Alternative name(s):
2,3-dihydro-2,3-dihydroxybiphenyl dehydrogenase
2,3-dihydroxy-4-phenylhexa-4,6-diene dehydrogenase
Biphenyl-2,3-dihydro-2,3-diol dehydrogenase
Biphenyl-cis-diol dehydrogenase

Gene names

Name:

bphB

Organism

Pseudomonas sp. (strain KKS102)

Taxonomic identifier

307 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria

Protein attributes

Sequence length	276 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Cis-3-phenylcyclohexa-3,5-diene-1,2-diol + NAD+ = biphenyl-2,3-diol + NADH.
Pathway	Xenobiotic degradation; biphenyl degradation; 2-hydroxy-2,4-pentadienoate and benzoate from biphenyl: step 2/4.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 276	276	Cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase		PRO_0000054534
Regions
	Nucleotide binding	9 – 33	25	NAD By similarity
Sites
	Active site	155	1	Proton acceptor By similarity
	Binding site	142	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P50206 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 3C000F5EA72F44D1	FASTA	276	28,933
	10 20 30 40 50 60 MQLNNEVALV TGGGSGLGRA IVDRFVAEGA RVAVLDKSAA RLQELQAAHG AKVLGIEGDV 70 80 90 100 110 120 RVLADHQKAA RECVAAFGKI DCLIPNAGIW DYSMPLVDIP DDRIDAAFDE VFHINVKGYL 130 140 150 160 170 180 LAVKACLPAL VQSRGSVVFT ISNAGFYPNG GGPLYTGAKH AVVGMVRELA YELAPHVRVN 190 200 210 220 230 240 GVAPGGMSTD LRGPASLGMA NQAISSVPLG EMLTSVLPVG RMPVRAEYTG AYVFFATRGD 250 260 270 TFPTTGALLN HDGGMGVRGF FEATGGKDLP QKLRLS

References

[1]	"Identification of the bphA and bphB genes of Pseudomonas sp. strains KKS102 involved in degradation of biphenyl and polychlorinated biphenyls." Fukuda M., Yasukochi Y., Kikuchi Y., Nagata Y., Kimbara K., Horiuchi H., Takagi M., Yano K. Biochem. Biophys. Res. Commun. 202:850-856(1994) [PubMed: 8048958] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D17319 Genomic DNA. Translation: BAA04140.1.
PIR	JC2441.
3D structure databases
ProteinModelPortal	P50206.
SMR	P50206. Positions 1-275.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR017711. BphB_TodD. IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR020904. Sc_DH/Rdtase_CS. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
TIGRFAMs	TIGR03325. BphB_TodD. 1 hit.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BPHB_PSES1

Accession

Primary (citable) accession number: P50206

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program