Reviewed, UniProtKB/Swiss-Prot P50205 (PHBB_RHIME)

Names and origin

Protein names

Recommended name:
    Acetoacetyl-CoA reductase
    EC=1.1.1.36

Gene names

Name:	phbB
Ordered Locus Names:	R03261
ORF Names:	SMc03878

Organism

Rhizobium meliloti (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

382 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	241 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(R)-3-hydroxyacyl-CoA + NADP+ = 3-oxoacyl-CoA + NADPH.
Pathway	Biopolymer metabolism; poly-(R)-3-hydroxybutanoate biosynthesis.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	PHB biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW poly-hydroxybutyrate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetoacetyl-CoA reductase activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 241	241	Acetoacetyl-CoA reductase		PRO_0000054750
Regions
	Nucleotide binding	7 – 31	25	NADP By similarity
Sites
	Active site	147	1	Proton acceptor By similarity
	Binding site	134	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P50205-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 025C43AF1900B1F0	FASTA	241	25,370
	10 20 30 40 50 60 MSRVALVTGG SRGIGAAICV ALKAAGYKVA ANYAGNDERA KAFEQESGIP VYKWDVSSYQ 70 80 90 100 110 120 ACVDGIARVE ADLGPVDILV NNAGITRDAM FHKMTPEQWG EVIGTNLTGV FNMTHPLWSG 130 140 150 160 170 180 MRDRGFGRIV NISSINGQKG QMGQVNYSAA KAGDLGLTKA LAQEGAAKGI TVNAICPGYI 190 200 210 220 230 240 GTEMVRAVPE KVLNERIIPQ IPVGRLGEPE EVARCVVFLA SDDAGFITGS TISANGGQYF A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Poly-beta-hydroxybutyrate (PHB) biosynthetic genes in Rhizobium meliloti 41." Tombolini R., Povolo S., Buson A., Squartini A., Nuti M.P. Microbiology 141:2553-2559(1995) [PubMed: 7582015] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 41.
[2]	"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021." Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F. Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed: 11481430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U17226 Genomic DNA. Translation: AAA90983.1. AL591688 Genomic DNA. Translation: CAC47840.1.
RefSeq	NP_387367.1.
3D structure databases
SMR	P50205. Positions 2-239.
ModBase	Search...
Genome annotation databases
GeneID	1234955.
GenomeReviews	Gene locus R03261 in contig AL591688_GR.
KEGG	sme:SMc03878.
NMPDR	fig|266834.1.peg.4555.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG750976.
OMA	SVNGGQY.
Enzyme and pathway databases
BioCyc	SMEL266834:SMC03878-MONOMER.
BRENDA	1.1.1.36. 142.
Family and domain databases
InterPro	IPR011283. Acetoacetyl-CoA_reductase. IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR020904. Sc_DH/Rdtase_CS. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
TIGRFAMs	TIGR01829. AcAcCoA_reduct. 1 hit.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PHBB_RHIME

Accession

Primary (citable) accession number: P50205

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	February 9, 2010
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)