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Protein

Acetoacetyl-CoA reductase

Gene

phbB

Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

(R)-3-hydroxyacyl-CoA + NADP+ = 3-oxoacyl-CoA + NADPH.

Pathway:ipoly-(R)-3-hydroxybutanoate biosynthesis

This protein is involved in the pathway poly-(R)-3-hydroxybutanoate biosynthesis, which is part of Biopolymer metabolism.
View all proteins of this organism that are known to be involved in the pathway poly-(R)-3-hydroxybutanoate biosynthesis and in Biopolymer metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei39 – 391NADPBy similarity
Binding sitei88 – 881SubstrateBy similarity
Active sitei147 – 1471Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 143NADPBy similarity
Nucleotide bindingi82 – 865NADPBy similarity
Nucleotide bindingi177 – 1804NADPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

PHB biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciSMEL266834:GJF6-3352-MONOMER.
UniPathwayiUPA00917.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetoacetyl-CoA reductase (EC:1.1.1.36)
Gene namesi
Name:phbB
Ordered Locus Names:R03261
ORF Names:SMc03878
OrganismiRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Taxonomic identifieri266834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
ProteomesiUP000001976 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 241241Acetoacetyl-CoA reductasePRO_0000054750Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi266834.SMc03878.

Structurei

3D structure databases

ProteinModelPortaliP50205.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni141 – 1444Substrate bindingBy similarity
Regioni178 – 1792Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1028.
KOiK00023.
OMAiGESTCHE.
OrthoDBiEOG6N3CR8.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR011283. Acetoacetyl-CoA_reductase.
IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
TIGRFAMsiTIGR01829. AcAcCoA_reduct. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50205-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRVALVTGG SRGIGAAICV ALKAAGYKVA ANYAGNDERA KAFEQESGIP
60 70 80 90 100
VYKWDVSSYQ ACVDGIARVE ADLGPVDILV NNAGITRDAM FHKMTPEQWG
110 120 130 140 150
EVIGTNLTGV FNMTHPLWSG MRDRGFGRIV NISSINGQKG QMGQVNYSAA
160 170 180 190 200
KAGDLGLTKA LAQEGAAKGI TVNAICPGYI GTEMVRAVPE KVLNERIIPQ
210 220 230 240
IPVGRLGEPE EVARCVVFLA SDDAGFITGS TISANGGQYF A
Length:241
Mass (Da):25,370
Last modified:October 1, 1996 - v1
Checksum:i025C43AF1900B1F0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17226 Genomic DNA. Translation: AAA90983.1.
AL591688 Genomic DNA. Translation: CAC47840.1.
RefSeqiNP_387367.1. NC_003047.1.
WP_003535773.1. NC_003047.1.

Genome annotation databases

EnsemblBacteriaiCAC47840; CAC47840; SMc03878.
GeneIDi1234955.
KEGGisme:SMc03878.
PATRICi23636186. VBISinMel96828_4815.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17226 Genomic DNA. Translation: AAA90983.1.
AL591688 Genomic DNA. Translation: CAC47840.1.
RefSeqiNP_387367.1. NC_003047.1.
WP_003535773.1. NC_003047.1.

3D structure databases

ProteinModelPortaliP50205.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi266834.SMc03878.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC47840; CAC47840; SMc03878.
GeneIDi1234955.
KEGGisme:SMc03878.
PATRICi23636186. VBISinMel96828_4815.

Phylogenomic databases

eggNOGiCOG1028.
KOiK00023.
OMAiGESTCHE.
OrthoDBiEOG6N3CR8.

Enzyme and pathway databases

UniPathwayiUPA00917.
BioCyciSMEL266834:GJF6-3352-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR011283. Acetoacetyl-CoA_reductase.
IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
TIGRFAMsiTIGR01829. AcAcCoA_reduct. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Poly-beta-hydroxybutyrate (PHB) biosynthetic genes in Rhizobium meliloti 41."
    Tombolini R., Povolo S., Buson A., Squartini A., Nuti M.P.
    Microbiology 141:2553-2559(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 41.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1021.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1021.

Entry informationi

Entry nameiPHBB_RHIME
AccessioniPrimary (citable) accession number: P50205
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 22, 2015
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.