Reviewed, UniProtKB/Swiss-Prot P50199 (GNO_GLUOX)

Names and origin

Protein names

Recommended name:
    Gluconate 5-dehydrogenase
    EC=1.1.1.69
Alternative name(s):
    5-keto-D-gluconate 5-reductase

Gene names

Name:	gno
Ordered Locus Names:	GOX2187

Organism

Gluconobacter oxydans (Gluconobacter suboxydans) [Complete proteome] [HAMAP]

Taxonomic identifier

442 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Gluconobacter

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the non-phosphorylative, ketogenic oxidation of glucose and oxidizes gluconate to 5-ketogluconate. Dependent on NADP, almost inactive with NAD.
Catalytic activity	D-gluconate + NAD(P)(+) = 5-dehydro-D-gluconate + NAD(P)H.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro gluconate 5-dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 256	256	Gluconate 5-dehydrogenase		PRO_0000054701
Regions
	Nucleotide binding	15 – 39	25	NADP By similarity
Sites
	Active site	160	1	Proton acceptor By similarity
	Binding site	147	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P50199-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 38B03C0399C0A07A	FASTA	256	27,256
	10 20 30 40 50 60 MSHPDLFSLS GARALVTGAS RGIGLTLAKG LARYGAEVVL NGRNAESLDS AQSGFEAEGL 70 80 90 100 110 120 KASTAVFDVT DQDAVIDGVA AIERDMGPID ILINNAGIQR RAPLEEFSRK DWDDLMSTNV 130 140 150 160 170 180 NAVFFVGQAV ARHMIPRGRG KIVNICSVQS ELARPGIAPY TATKGAVKNL TKGMATDWGR 190 200 210 220 230 240 HGLQINGLAP GYFATEMTER LVADEEFTDW LCKRTPAGRW GQVEELVGAA VFLSSRASSF 250 VNGQVLMVDG GITVSL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Biochemical characterization and sequence analysis of the gluconate:NADP 5-oxidoreductase gene from Gluconobacter oxydans." Klasen R., Bringer-Meyer S., Sahm H. J. Bacteriol. 177:2637-2643(1995) [PubMed: 7751271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 3-19, CHARACTERIZATION. Strain: ATCC 19357 / DSM 3503 / IFO 14819 / LMG 1408 / NCIB 9013.
[2]	"Complete genome sequence of the acetic acid bacterium Gluconobacter oxydans." Prust C., Hoffmeister M., Liesegang H., Wiezer A., Fricke W.F., Ehrenreich A., Gottschalk G., Deppenmeier U. Nat. Biotechnol. 23:195-200(2005) [PubMed: 15665824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 621H.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X80019 Genomic DNA. Translation: CAA56322.1. CP000009 Genomic DNA. Translation: AAW61923.1.
PIR	A57149.
RefSeq	YP_192579.1.
3D structure databases
HSSP	HSSP built from PDB template 1CYD based on UniProtKB P08074.
ModBase	Search...
Genome annotation databases
GeneID	3250506.
GenomeReviews	Gene locus GOX2187 in contig CP000009_GR.
KEGG	gox:GOX2187.
NMPDR	fig|290633.1.peg.2124.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P50199.
Enzyme and pathway databases
BioCyc	GOXY290633:GOX2187-MON.
Family and domain databases
InterPro	IPR002198. DHase_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DHase. [Graphical view]
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GNO_GLUOX

Accession

Primary (citable) accession number: P50199
Secondary accession number(s): Q5FNX3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 25, 2008
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)