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Protein

Modification methylase Eco47II

Gene

eco47IIM

Organism
Escherichia coli
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GGNCC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the Eco47II endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei153 – 1531PROSITE-ProRule annotation

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: InterPro

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
  2. regulation of transcription, DNA-templated Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3372. M.Eco47II.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase Eco47II (EC:2.1.1.37)
Short name:
M.Eco47II
Alternative name(s):
Cytosine-specific methyltransferase Eco47II
Gene namesi
Name:eco47IIM
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 417417Modification methylase Eco47IIPRO_0000087875Add
BLAST

Proteomic databases

PRIDEiP50196.

Structurei

3D structure databases

ProteinModelPortaliP50196.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini81 – 414334SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR009061. DNA-bd_dom_put.
IPR000551. MerR-type_HTH_dom.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF00376. MerR. 1 hit.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF46955. SSF46955. 1 hit.
SSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50196-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLAWLLNMLK EEFSLSEVAD ILGVSKETLR RWDTAGKLVS QRNDENNYRF
60 70 80 90 100
YKKEQLKNFE QAQFLFKSQW PDETKISNNV YTVLELFAGA GGMALGLEKA
110 120 130 140 150
GLKSVLLNEI DSHACKTLRK NRPEWNVVEG DVSQVDFTPY RNTVDVLAGG
160 170 180 190 200
FPCQAFSYAG KKLGFEDTRG TLFFEFARAA KEINPKVLLA ENVRGLLNHD
210 220 230 240 250
AGRTLETIKN IITDLGYTLF EPRVLKAIFY KVPQKRERLI IVAVRNDLAD
260 270 280 290 300
GIDYEWPSSY NKILTLKDAL KKGELYDSDV PESEGQKYPK RKAEILSMVP
310 320 330 340 350
PGGYWRDLPE DIQKEYMLKS FYLGGGKTGM ARRLSWDEPS LTLTCAPAQK
360 370 380 390 400
QTERCHPEET RPLTVREYAR IQTFPDEWVF EGPMSAKYKQ IGNAVPVNLS
410
FAVGKSVVHL LDKINKR
Length:417
Mass (Da):47,513
Last modified:September 30, 1996 - v1
Checksum:iC399A49E00BDAFD3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82105 Genomic DNA. Translation: CAA57629.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82105 Genomic DNA. Translation: CAA57629.1.

3D structure databases

ProteinModelPortaliP50196.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi3372. M.Eco47II.

Proteomic databases

PRIDEiP50196.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR009061. DNA-bd_dom_put.
IPR000551. MerR-type_HTH_dom.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF00376. MerR. 1 hit.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF46955. SSF46955. 1 hit.
SSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and characterization of the unusual restriction-modification system comprising two restriction endonucleases and one methyltransferase."
    Stankevicius K., Povilionis P., Lubys A., Menkevicius S., Janulaitis A.
    Gene 157:49-53(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: RFL47.

Entry informationi

Entry nameiMTE8_ECOLX
AccessioniPrimary (citable) accession number: P50196
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 30, 1996
Last sequence update: September 30, 1996
Last modified: January 6, 2015
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.