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Protein

Modification methylase Eco47II

Gene

eco47IIM

Organism
Escherichia coli
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GGNCC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the Eco47II endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei153PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processRestriction system
LigandS-adenosyl-L-methionine

Protein family/group databases

REBASEi3372 M.Eco47II

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase Eco47II (EC:2.1.1.37)
Short name:
M.Eco47II
Alternative name(s):
Cytosine-specific methyltransferase Eco47II
Gene namesi
Name:eco47IIM
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000878751 – 417Modification methylase Eco47IIAdd BLAST417

Structurei

3D structure databases

ProteinModelPortaliP50196
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini81 – 414SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd BLAST334

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation

Family and domain databases

InterProiView protein in InterPro
IPR018117 C5_DNA_meth_AS
IPR001525 C5_MeTfrase
IPR031303 C5_meth_CS
IPR009061 DNA-bd_dom_put_sf
IPR000551 MerR-type_HTH_dom
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF00145 DNA_methylase, 1 hit
PF13411 MerR_1, 1 hit
PRINTSiPR00105 C5METTRFRASE
SUPFAMiSSF46955 SSF46955, 1 hit
SSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00675 dcm, 1 hit
PROSITEiView protein in PROSITE
PS00094 C5_MTASE_1, 1 hit
PS00095 C5_MTASE_2, 1 hit
PS51679 SAM_MT_C5, 1 hit

Sequencei

Sequence statusi: Complete.

P50196-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLAWLLNMLK EEFSLSEVAD ILGVSKETLR RWDTAGKLVS QRNDENNYRF
60 70 80 90 100
YKKEQLKNFE QAQFLFKSQW PDETKISNNV YTVLELFAGA GGMALGLEKA
110 120 130 140 150
GLKSVLLNEI DSHACKTLRK NRPEWNVVEG DVSQVDFTPY RNTVDVLAGG
160 170 180 190 200
FPCQAFSYAG KKLGFEDTRG TLFFEFARAA KEINPKVLLA ENVRGLLNHD
210 220 230 240 250
AGRTLETIKN IITDLGYTLF EPRVLKAIFY KVPQKRERLI IVAVRNDLAD
260 270 280 290 300
GIDYEWPSSY NKILTLKDAL KKGELYDSDV PESEGQKYPK RKAEILSMVP
310 320 330 340 350
PGGYWRDLPE DIQKEYMLKS FYLGGGKTGM ARRLSWDEPS LTLTCAPAQK
360 370 380 390 400
QTERCHPEET RPLTVREYAR IQTFPDEWVF EGPMSAKYKQ IGNAVPVNLS
410
FAVGKSVVHL LDKINKR
Length:417
Mass (Da):47,513
Last modified:October 1, 1996 - v1
Checksum:iC399A49E00BDAFD3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82105 Genomic DNA Translation: CAA57629.1

Similar proteinsi

Entry informationi

Entry nameiMTE8_ECOLX
AccessioniPrimary (citable) accession number: P50196
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health