Reviewed, UniProtKB/Swiss-Prot P50196 (MTE8_ECOLX)

Names and origin

Protein names

Recommended name:
    Modification methylase Eco47II
      Short name=M.Eco47II
    EC=2.1.1.37
Alternative name(s):
    Cytosine-specific methyltransferase Eco47II

Gene names

Name:

eco47IIM

Organism

Escherichia coli

Taxonomic identifier

562 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GGNCC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the Eco47II endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Molecular function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	Modification methylase Eco47II		PRO_0000087875
Sites
	Active site	153	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P50196-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: C399A49E00BDAFD3	FASTA	417	47,513
	10 20 30 40 50 60 MLAWLLNMLK EEFSLSEVAD ILGVSKETLR RWDTAGKLVS QRNDENNYRF YKKEQLKNFE 70 80 90 100 110 120 QAQFLFKSQW PDETKISNNV YTVLELFAGA GGMALGLEKA GLKSVLLNEI DSHACKTLRK 130 140 150 160 170 180 NRPEWNVVEG DVSQVDFTPY RNTVDVLAGG FPCQAFSYAG KKLGFEDTRG TLFFEFARAA 190 200 210 220 230 240 KEINPKVLLA ENVRGLLNHD AGRTLETIKN IITDLGYTLF EPRVLKAIFY KVPQKRERLI 250 260 270 280 290 300 IVAVRNDLAD GIDYEWPSSY NKILTLKDAL KKGELYDSDV PESEGQKYPK RKAEILSMVP 310 320 330 340 350 360 PGGYWRDLPE DIQKEYMLKS FYLGGGKTGM ARRLSWDEPS LTLTCAPAQK QTERCHPEET 370 380 390 400 410 RPLTVREYAR IQTFPDEWVF EGPMSAKYKQ IGNAVPVNLS FAVGKSVVHL LDKINKR

References

[1]

"Cloning and characterization of the unusual restriction-modification system comprising two restriction endonucleases and one methyltransferase." Stankevicius K., Povilionis P., Lubys A., Menkevicius S., Janulaitis A. Gene 157:49-53(1995) [PubMed: 7607524] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: RFL47.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X82105 Genomic DNA. Translation: CAA57629.1.
3D structure databases
SMR	P50196. Positions 12-67, 80-412.
ModBase	Search...
Protein family/group databases
REBASE	3372. M.Eco47II.
Enzyme and pathway databases
BRENDA	2.1.1.37. 246.
Family and domain databases
InterPro	IPR001525. C5_DNA_meth. IPR018117. C5_DNA_meth_AS. IPR009061. DNA-bd_dom_put. IPR000551. HTH_MerR. [Graphical view]
PANTHER	PTHR10629. C5_DNA_meth. 1 hit.
Pfam	PF00145. DNA_methylase. 1 hit. PF00376. MerR. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTE8_ECOLX

Accession

Primary (citable) accession number: P50196

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	February 9, 2010
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)