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P50195

- T2E8_ECOLX

UniProt

P50195 - T2E8_ECOLX

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Protein
Type-2 restriction enzyme Eco47II
Gene
eco47IIR
Organism
Escherichia coli
Status
Reviewed - Annotation score: 2 out of 5 - Protein predictedi

Functioni

Recognizes the double-stranded sequence GGNCC and cleaves after G-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi931. Eco47II.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme Eco47II (EC:3.1.21.4)
Short name:
R.Eco47II
Alternative name(s):
Endonuclease Eco47II
Type II restriction enzyme Eco47II
Gene namesi
Name:eco47IIR
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 239239Type-2 restriction enzyme Eco47II
PRO_0000077307Add
BLAST

Family & Domainsi

Family and domain databases

InterProiIPR019057. Restrct_endonuc_II_Eco47II.
[Graphical view]
PfamiPF09553. RE_Eco47II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50195-1 [UniParc]FASTAAdd to Basket

« Hide

MSLLEYISDE DLFNEVETLL TKAKKKKDAA EKTFTSNVID PFGALFEAPG    50
FSSHEEWRNS ELARQQQKTI QNHVGTFHQK ILGHVEGWRD MGIGGIVDLL 100
NEERRIIAEV KNKYSTVTGG DLADKYKGLD ELVSPKHSRF KDYCAYFVNI 150
IPRKPTRYNS PFTPSNKGSG TLCPSNPNIR IIDGASFYEL VTGRPDALQE 200
LHSALPHAIE YILSERLGQQ GFSIPDKDSF IKYFGLAYG 239
Length:239
Mass (Da):26,921
Last modified:October 1, 1996 - v1
Checksum:i8F31F81A0127F3CE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X82105 Genomic DNA. Translation: CAA57628.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X82105 Genomic DNA. Translation: CAA57628.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 931. Eco47II.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR019057. Restrct_endonuc_II_Eco47II.
[Graphical view ]
Pfami PF09553. RE_Eco47II. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and characterization of the unusual restriction-modification system comprising two restriction endonucleases and one methyltransferase."
    Stankevicius K., Povilionis P., Lubys A., Menkevicius S., Janulaitis A.
    Gene 157:49-53(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: RFL47.

Entry informationi

Entry nameiT2E8_ECOLX
AccessioniPrimary (citable) accession number: P50195
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

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