Reviewed, UniProtKB/Swiss-Prot P50195 (T2E8_ECOLX)

Names and origin

Protein names

Recommended name:
    Type-2 restriction enzyme Eco47II
      Short name=R.Eco47II
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme Eco47II
    Endonuclease Eco47II

Gene names

Name:

eco47IIR

Organism

Escherichia coli

Taxonomic identifier

562 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	239 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Predicted.

General annotation (Comments)

Function	Recognizes the double-stranded sequence GGNCC and cleaves after G-1.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 239	239	Type-2 restriction enzyme Eco47II		PRO_0000077307

Sequences

	Sequence		Length	Mass (Da)
	P50195-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 8F31F81A0127F3CE	FASTA	239	26,921
	10 20 30 40 50 60 MSLLEYISDE DLFNEVETLL TKAKKKKDAA EKTFTSNVID PFGALFEAPG FSSHEEWRNS 70 80 90 100 110 120 ELARQQQKTI QNHVGTFHQK ILGHVEGWRD MGIGGIVDLL NEERRIIAEV KNKYSTVTGG 130 140 150 160 170 180 DLADKYKGLD ELVSPKHSRF KDYCAYFVNI IPRKPTRYNS PFTPSNKGSG TLCPSNPNIR 190 200 210 220 230 IIDGASFYEL VTGRPDALQE LHSALPHAIE YILSERLGQQ GFSIPDKDSF IKYFGLAYG

References

[1]

"Cloning and characterization of the unusual restriction-modification system comprising two restriction endonucleases and one methyltransferase." Stankevicius K., Povilionis P., Lubys A., Menkevicius S., Janulaitis A. Gene 157:49-53(1995) [PubMed: 7607524] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: RFL47.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X82105 Genomic DNA. Translation: CAA57628.1.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	931. Eco47II.
Enzyme and pathway databases
BRENDA	3.1.21.4. 246.
Family and domain databases
InterPro	IPR019057. Restrict_endonuc_II_Eco47II. [Graphical view]
Pfam	PF09553. RE_Eco47II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T2E8_ECOLX

Accession

Primary (citable) accession number: P50195

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)