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P50195

- T2E8_ECOLX

UniProt

P50195 - T2E8_ECOLX

Protein

Type-2 restriction enzyme Eco47II

Gene

eco47IIR

Organism
Escherichia coli
Status
Reviewed - Annotation score: 2 out of 5- Protein predictedi
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    • History
      Entry version 42 (16 Apr 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    • Comment

    Functioni

    Recognizes the double-stranded sequence GGNCC and cleaves after G-1.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Protein family/group databases

    REBASEi931. Eco47II.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme Eco47II (EC:3.1.21.4)
    Short name:
    R.Eco47II
    Alternative name(s):
    Endonuclease Eco47II
    Type II restriction enzyme Eco47II
    Gene namesi
    Name:eco47IIR
    OrganismiEscherichia coli
    Taxonomic identifieri562 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 239239Type-2 restriction enzyme Eco47IIPRO_0000077307Add
    BLAST

    Family & Domainsi

    Family and domain databases

    InterProiIPR019057. Restrct_endonuc_II_Eco47II.
    [Graphical view]
    PfamiPF09553. RE_Eco47II. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P50195-1 [UniParc]FASTAAdd to Basket

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    MSLLEYISDE DLFNEVETLL TKAKKKKDAA EKTFTSNVID PFGALFEAPG    50
    FSSHEEWRNS ELARQQQKTI QNHVGTFHQK ILGHVEGWRD MGIGGIVDLL 100
    NEERRIIAEV KNKYSTVTGG DLADKYKGLD ELVSPKHSRF KDYCAYFVNI 150
    IPRKPTRYNS PFTPSNKGSG TLCPSNPNIR IIDGASFYEL VTGRPDALQE 200
    LHSALPHAIE YILSERLGQQ GFSIPDKDSF IKYFGLAYG 239
    Length:239
    Mass (Da):26,921
    Last modified:October 1, 1996 - v1
    Checksum:i8F31F81A0127F3CE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X82105 Genomic DNA. Translation: CAA57628.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X82105 Genomic DNA. Translation: CAA57628.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 931. Eco47II.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR019057. Restrct_endonuc_II_Eco47II.
    [Graphical view ]
    Pfami PF09553. RE_Eco47II. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of the unusual restriction-modification system comprising two restriction endonucleases and one methyltransferase."
      Stankevicius K., Povilionis P., Lubys A., Menkevicius S., Janulaitis A.
      Gene 157:49-53(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: RFL47.

    Entry informationi

    Entry nameiT2E8_ECOLX
    AccessioniPrimary (citable) accession number: P50195
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: April 16, 2014
    This is version 42 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3