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P50194

- T2E7_ECOLX

UniProt

P50194 - T2E7_ECOLX

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Protein
Type-2 restriction enzyme Eco47I
Gene
eco47IR
Organism
Escherichia coli
Status
Reviewed - Annotation score: 2 out of 5 - Protein predictedi

Functioni

Recognizes the double-stranded sequence GGWCC and cleaves after G-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi930. Eco47I.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme Eco47I (EC:3.1.21.4)
Short name:
R.Eco47I
Alternative name(s):
Endonuclease Eco47I
Type II restriction enzyme Eco47I
Gene namesi
Name:eco47IR
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 230230Type-2 restriction enzyme Eco47I
PRO_0000077306Add
BLAST

Family & Domainsi

Family and domain databases

InterProiIPR019070. Restrct_endonuc_II_SinI.
[Graphical view]
PfamiPF09570. RE_SinI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50194-1 [UniParc]FASTAAdd to Basket

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MSKETSFVKN AEELAKQKMD AINPELSSKF KFLIKFLSQF PEACSKPRSK    50
KMQNKVGQEE HIEYLARSFH ESRLPRKPTP PTTVPDEVVS IVLNISFNIQ 100
PENLERIKEE HRLSMAAENI VGDLLERYLA EKLEPSGWIW CSGTSVKAVD 150
FIHYDEKNNE WNLLQVKNRD NTENSSSSKI RDNTTIKKWF RTYSQRDATN 200
WDNFPDEVSS KNLNEEDFRA FVKNYLVKII 230
Length:230
Mass (Da):26,864
Last modified:October 1, 1996 - v1
Checksum:iAA3621FF506DFB11
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X82105 Genomic DNA. Translation: CAA57630.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X82105 Genomic DNA. Translation: CAA57630.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 930. Eco47I.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR019070. Restrct_endonuc_II_SinI.
[Graphical view ]
Pfami PF09570. RE_SinI. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and characterization of the unusual restriction-modification system comprising two restriction endonucleases and one methyltransferase."
    Stankevicius K., Povilionis P., Lubys A., Menkevicius S., Janulaitis A.
    Gene 157:49-53(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: RFL47.

Entry informationi

Entry nameiT2E7_ECOLX
AccessioniPrimary (citable) accession number: P50194
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

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