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Reviewed, UniProtKB/Swiss-Prot P50194 (T2E7_ECOLX)

Last modified June 16, 2009. Version 32. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Type-2 restriction enzyme Eco47I
      Short name=R.Eco47I
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme Eco47I
    Endonuclease Eco47I
Gene names
Name: eco47IR
OrganismEscherichia coli
Taxonomic identifier562 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length230 AA.
Sequence statusComplete.
Protein existencePredicted.

General annotation (Comments)

Function

Recognizes the double-stranded sequence GGWCC and cleaves after G-1.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 230230Type-2 restriction enzyme Eco47I
PRO_0000077306

Sequences

Sequence LengthMass (Da)Tools
P50194-1 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: AA3621FF506DFB11

FASTA23026,864
        10         20         30         40         50         60 
MSKETSFVKN AEELAKQKMD AINPELSSKF KFLIKFLSQF PEACSKPRSK KMQNKVGQEE 

        70         80         90        100        110        120 
HIEYLARSFH ESRLPRKPTP PTTVPDEVVS IVLNISFNIQ PENLERIKEE HRLSMAAENI 

       130        140        150        160        170        180 
VGDLLERYLA EKLEPSGWIW CSGTSVKAVD FIHYDEKNNE WNLLQVKNRD NTENSSSSKI 

       190        200        210        220        230 
RDNTTIKKWF RTYSQRDATN WDNFPDEVSS KNLNEEDFRA FVKNYLVKII 

« Hide

References

[1]"Cloning and characterization of the unusual restriction-modification system comprising two restriction endonucleases and one methyltransferase."
Stankevicius K., Povilionis P., Lubys A., Menkevicius S., Janulaitis A.
Gene 157:49-53(1995) [PubMed: 7607524] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: RFL47.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X82105 Genomic DNA. Translation: CAA57630.1.

3D structure databases

ModBaseSearch...

Protein family/group databases

REBASE930. Eco47I.

Enzyme and pathway databases

BRENDA3.1.21.4. 246.

Family and domain databases

InterProIPR019070. Restrict_endonuc_II_SinI.
[Graphical view]
PfamPF09570. RE_SinI. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT2E7_ECOLX
AccessionPrimary (citable) accession number: P50194
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 16, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents