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Protein

Type-2 restriction enzyme Eco47I

Gene

eco47IR

Organism
Escherichia coli
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein predictedi

Functioni

Recognizes the double-stranded sequence GGWCC and cleaves after G-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi930. Eco47I.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme Eco47I (EC:3.1.21.4)
Short name:
R.Eco47I
Alternative name(s):
Endonuclease Eco47I
Type II restriction enzyme Eco47I
Gene namesi
Name:eco47IR
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 230230Type-2 restriction enzyme Eco47IPRO_0000077306Add
BLAST

Family & Domainsi

Family and domain databases

InterProiIPR019070. Restrct_endonuc_II_SinI.
[Graphical view]
PfamiPF09570. RE_SinI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50194-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKETSFVKN AEELAKQKMD AINPELSSKF KFLIKFLSQF PEACSKPRSK
60 70 80 90 100
KMQNKVGQEE HIEYLARSFH ESRLPRKPTP PTTVPDEVVS IVLNISFNIQ
110 120 130 140 150
PENLERIKEE HRLSMAAENI VGDLLERYLA EKLEPSGWIW CSGTSVKAVD
160 170 180 190 200
FIHYDEKNNE WNLLQVKNRD NTENSSSSKI RDNTTIKKWF RTYSQRDATN
210 220 230
WDNFPDEVSS KNLNEEDFRA FVKNYLVKII
Length:230
Mass (Da):26,864
Last modified:October 1, 1996 - v1
Checksum:iAA3621FF506DFB11
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82105 Genomic DNA. Translation: CAA57630.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82105 Genomic DNA. Translation: CAA57630.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi930. Eco47I.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR019070. Restrct_endonuc_II_SinI.
[Graphical view]
PfamiPF09570. RE_SinI. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and characterization of the unusual restriction-modification system comprising two restriction endonucleases and one methyltransferase."
    Stankevicius K., Povilionis P., Lubys A., Menkevicius S., Janulaitis A.
    Gene 157:49-53(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: RFL47.

Entry informationi

Entry nameiT2E7_ECOLX
AccessioniPrimary (citable) accession number: P50194
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.