Reviewed, UniProtKB/Swiss-Prot P50194 (T2E7_ECOLX)

Names and origin

Protein names

Recommended name:
    Type-2 restriction enzyme Eco47I
      Short name=R.Eco47I
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme Eco47I
    Endonuclease Eco47I

Gene names

Name:

eco47IR

Organism

Escherichia coli

Taxonomic identifier

562 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	230 AA.
Sequence status	Complete.
Protein existence	Predicted.

General annotation (Comments)

Function	Recognizes the double-stranded sequence GGWCC and cleaves after G-1.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 230	230	Type-2 restriction enzyme Eco47I		PRO_0000077306

Sequences

	Sequence		Length	Mass (Da)
	P50194-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: AA3621FF506DFB11	FASTA	230	26,864
	10 20 30 40 50 60 MSKETSFVKN AEELAKQKMD AINPELSSKF KFLIKFLSQF PEACSKPRSK KMQNKVGQEE 70 80 90 100 110 120 HIEYLARSFH ESRLPRKPTP PTTVPDEVVS IVLNISFNIQ PENLERIKEE HRLSMAAENI 130 140 150 160 170 180 VGDLLERYLA EKLEPSGWIW CSGTSVKAVD FIHYDEKNNE WNLLQVKNRD NTENSSSSKI 190 200 210 220 230 RDNTTIKKWF RTYSQRDATN WDNFPDEVSS KNLNEEDFRA FVKNYLVKII

References

[1]

"Cloning and characterization of the unusual restriction-modification system comprising two restriction endonucleases and one methyltransferase." Stankevicius K., Povilionis P., Lubys A., Menkevicius S., Janulaitis A. Gene 157:49-53(1995) [PubMed: 7607524] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: RFL47.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X82105 Genomic DNA. Translation: CAA57630.1.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	930. Eco47I.
Enzyme and pathway databases
BRENDA	3.1.21.4. 246.
Family and domain databases
InterPro	IPR019070. Restrict_endonuc_II_SinI. [Graphical view]
Pfam	PF09570. RE_SinI. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T2E7_ECOLX

Accession

Primary (citable) accession number: P50194

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)