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Protein

Modification methylase HphIB

Gene

hphIBM

Organism
Haemophilus parahaemolyticus
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

This methylase protects the DNA from cleavage by the HphI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3661. M2.HphI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase HphIB (EC:2.1.1.72)
Short name:
M.HphIB
Alternative name(s):
Adenine-specific methyltransferase HphIB
M.HphI(A)
Gene namesi
Name:hphIBM
Synonyms:hphIMA
OrganismiHaemophilus parahaemolyticus
Taxonomic identifieri735 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 336336Modification methylase HphIBPRO_0000087952Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP50193.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PRINTSiPR00505. D12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50193-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNPKYPKVN YIGNKEKIAE WICEQLPVDV RTIADVFSGG CSFSFEAKKR
60 70 80 90 100
GYQVIANDIL NINYQLALAL IVNNQEILTA CDVDFIFSNP PKSGFMTKNY
110 120 130 140 150
SDVFFFKEEC RELDAIRANI LKLNNTYKQA LAFALMRRAM IRKMPYSRFT
160 170 180 190 200
ISWEKVKQLR DEEYSYSKYG RRRAYHNQSF EFHFRENLNS YNQAVFNNGN
210 220 230 240 250
IHQAYNEDVF ELLDHIQADA VYLDPPYTGT MNNYFGFYGL LDSYMSGEIR
260 270 280 290 300
QPFDNHFMDK NQAVELFEKL IEKLKPFKYW LLSYNNVSRP NREELTAMLS
310 320 330
RNGRKVTVLE TPHVYKVTGK ENKQKHTELL FLVENR
Length:336
Mass (Da):39,609
Last modified:October 1, 1996 - v1
Checksum:i3BD144C7772C4FDF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85374 Genomic DNA. Translation: CAA59691.1.
PIRiS70708.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85374 Genomic DNA. Translation: CAA59691.1.
PIRiS70708.

3D structure databases

ProteinModelPortaliP50193.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi3661. M2.HphI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PRINTSiPR00505. D12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and analysis of the genes encoding the type IIS restriction-modification system HphI from Haemophilus parahaemolyticus."
    Lubys A., Lubiene J., Kulakauskkas S., Stankevicius K., Timinskas A., Janulaitis A.
    Nucleic Acids Res. 24:2760-2766(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 49700.

Entry informationi

Entry nameiMTHB_HAEPH
AccessioniPrimary (citable) accession number: P50193
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: January 7, 2015
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.