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P50193

- MTHB_HAEPH

UniProt

P50193 - MTHB_HAEPH

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Protein
Modification methylase HphIB
Gene
hphIBM, hphIMA
Organism
Haemophilus parahaemolyticus
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

This methylase protects the DNA from cleavage by the HphI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
  2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3661. M2.HphI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase HphIB (EC:2.1.1.72)
Short name:
M.HphIB
Alternative name(s):
Adenine-specific methyltransferase HphIB
M.HphI(A)
Gene namesi
Name:hphIBM
Synonyms:hphIMA
OrganismiHaemophilus parahaemolyticus
Taxonomic identifieri735 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 336336Modification methylase HphIB
PRO_0000087952Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP50193.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PRINTSiPR00505. D12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50193-1 [UniParc]FASTAAdd to Basket

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MNNPKYPKVN YIGNKEKIAE WICEQLPVDV RTIADVFSGG CSFSFEAKKR    50
GYQVIANDIL NINYQLALAL IVNNQEILTA CDVDFIFSNP PKSGFMTKNY 100
SDVFFFKEEC RELDAIRANI LKLNNTYKQA LAFALMRRAM IRKMPYSRFT 150
ISWEKVKQLR DEEYSYSKYG RRRAYHNQSF EFHFRENLNS YNQAVFNNGN 200
IHQAYNEDVF ELLDHIQADA VYLDPPYTGT MNNYFGFYGL LDSYMSGEIR 250
QPFDNHFMDK NQAVELFEKL IEKLKPFKYW LLSYNNVSRP NREELTAMLS 300
RNGRKVTVLE TPHVYKVTGK ENKQKHTELL FLVENR 336
Length:336
Mass (Da):39,609
Last modified:October 1, 1996 - v1
Checksum:i3BD144C7772C4FDF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X85374 Genomic DNA. Translation: CAA59691.1.
PIRiS70708.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X85374 Genomic DNA. Translation: CAA59691.1 .
PIRi S70708.

3D structure databases

ProteinModelPortali P50193.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3661. M2.HphI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 2 hits.
InterProi IPR002052. DNA_methylase_N6_adenine_CS.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF02086. MethyltransfD12. 1 hit.
[Graphical view ]
PRINTSi PR00505. D12N6MTFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS00092. N6_MTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and analysis of the genes encoding the type IIS restriction-modification system HphI from Haemophilus parahaemolyticus."
    Lubys A., Lubiene J., Kulakauskkas S., Stankevicius K., Timinskas A., Janulaitis A.
    Nucleic Acids Res. 24:2760-2766(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 49700.

Entry informationi

Entry nameiMTHB_HAEPH
AccessioniPrimary (citable) accession number: P50193
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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