P50193 (MTHB_HAEPH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase HphIB
Short name=M.HphIB
EC=2.1.1.72

Alternative name(s):
Adenine-specific methyltransferase HphIB
M.HphI(A)

Gene names

Name:	hphIBM
Synonyms:	hphIMA

Organism

Haemophilus parahaemolyticus

Taxonomic identifier

735 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This methylase protects the DNA from cleavage by the HphI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	nucleic acid binding Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	Modification methylase HphIB		PRO_0000087952

Sequences

	Sequence		Length	Mass (Da)
	P50193 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 3BD144C7772C4FDF	FASTA	336	39,609
	10 20 30 40 50 60 MNNPKYPKVN YIGNKEKIAE WICEQLPVDV RTIADVFSGG CSFSFEAKKR GYQVIANDIL 70 80 90 100 110 120 NINYQLALAL IVNNQEILTA CDVDFIFSNP PKSGFMTKNY SDVFFFKEEC RELDAIRANI 130 140 150 160 170 180 LKLNNTYKQA LAFALMRRAM IRKMPYSRFT ISWEKVKQLR DEEYSYSKYG RRRAYHNQSF 190 200 210 220 230 240 EFHFRENLNS YNQAVFNNGN IHQAYNEDVF ELLDHIQADA VYLDPPYTGT MNNYFGFYGL 250 260 270 280 290 300 LDSYMSGEIR QPFDNHFMDK NQAVELFEKL IEKLKPFKYW LLSYNNVSRP NREELTAMLS 310 320 330 RNGRKVTVLE TPHVYKVTGK ENKQKHTELL FLVENR

References

[1]

"Cloning and analysis of the genes encoding the type IIS restriction-modification system HphI from Haemophilus parahaemolyticus." Lubys A., Lubiene J., Kulakauskkas S., Stankevicius K., Timinskas A., Janulaitis A. Nucleic Acids Res. 24:2760-2766(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 49700.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X85374 Genomic DNA. Translation: CAA59691.1.
PIR	S70708.
3D structure databases
ProteinModelPortal	P50193.
ModBase	Search...
Protein family/group databases
REBASE	3661. M2.HphI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR012327. MeTrfase_D12. [Graphical view]
Pfam	PF02086. MethyltransfD12. 1 hit. [Graphical view]
PRINTS	PR00505. D12N6MTFRASE.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTHB_HAEPH

Accession

Primary (citable) accession number: P50193

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program