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P50193

- MTHB_HAEPH

UniProt

P50193 - MTHB_HAEPH

Protein

Modification methylase HphIB

Gene

hphIBM

Organism
Haemophilus parahaemolyticus
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 54 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    This methylase protects the DNA from cleavage by the HphI endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

    GO - Molecular functioni

    1. nucleic acid binding Source: InterPro
    2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3661. M2.HphI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase HphIB (EC:2.1.1.72)
    Short name:
    M.HphIB
    Alternative name(s):
    Adenine-specific methyltransferase HphIB
    M.HphI(A)
    Gene namesi
    Name:hphIBM
    Synonyms:hphIMA
    OrganismiHaemophilus parahaemolyticus
    Taxonomic identifieri735 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 336336Modification methylase HphIBPRO_0000087952Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP50193.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the N(4)/N(6)-methyltransferase family.Curated

    Family and domain databases

    Gene3Di3.40.50.150. 2 hits.
    InterProiIPR002052. DNA_methylase_N6_adenine_CS.
    IPR012327. MeTrfase_D12.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF02086. MethyltransfD12. 1 hit.
    [Graphical view]
    PRINTSiPR00505. D12N6MTFRASE.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS00092. N6_MTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P50193-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNNPKYPKVN YIGNKEKIAE WICEQLPVDV RTIADVFSGG CSFSFEAKKR    50
    GYQVIANDIL NINYQLALAL IVNNQEILTA CDVDFIFSNP PKSGFMTKNY 100
    SDVFFFKEEC RELDAIRANI LKLNNTYKQA LAFALMRRAM IRKMPYSRFT 150
    ISWEKVKQLR DEEYSYSKYG RRRAYHNQSF EFHFRENLNS YNQAVFNNGN 200
    IHQAYNEDVF ELLDHIQADA VYLDPPYTGT MNNYFGFYGL LDSYMSGEIR 250
    QPFDNHFMDK NQAVELFEKL IEKLKPFKYW LLSYNNVSRP NREELTAMLS 300
    RNGRKVTVLE TPHVYKVTGK ENKQKHTELL FLVENR 336
    Length:336
    Mass (Da):39,609
    Last modified:October 1, 1996 - v1
    Checksum:i3BD144C7772C4FDF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X85374 Genomic DNA. Translation: CAA59691.1.
    PIRiS70708.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X85374 Genomic DNA. Translation: CAA59691.1 .
    PIRi S70708.

    3D structure databases

    ProteinModelPortali P50193.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3661. M2.HphI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.150. 2 hits.
    InterProi IPR002052. DNA_methylase_N6_adenine_CS.
    IPR012327. MeTrfase_D12.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF02086. MethyltransfD12. 1 hit.
    [Graphical view ]
    PRINTSi PR00505. D12N6MTFRASE.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS00092. N6_MTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and analysis of the genes encoding the type IIS restriction-modification system HphI from Haemophilus parahaemolyticus."
      Lubys A., Lubiene J., Kulakauskkas S., Stankevicius K., Timinskas A., Janulaitis A.
      Nucleic Acids Res. 24:2760-2766(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 49700.

    Entry informationi

    Entry nameiMTHB_HAEPH
    AccessioniPrimary (citable) accession number: P50193
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 54 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3