Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P50193 (MTHB_HAEPH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase HphIB

Short name=M.HphIB
EC=2.1.1.72
Alternative name(s):
Adenine-specific methyltransferase HphIB
M.HphI(A)
Gene names
Name:hphIBM
Synonyms:hphIMA
OrganismHaemophilus parahaemolyticus
Taxonomic identifier735 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length336 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase protects the DNA from cleavage by the HphI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 336336Modification methylase HphIB
PRO_0000087952

Sequences

Sequence LengthMass (Da)Tools
P50193 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 3BD144C7772C4FDF

FASTA33639,609
        10         20         30         40         50         60 
MNNPKYPKVN YIGNKEKIAE WICEQLPVDV RTIADVFSGG CSFSFEAKKR GYQVIANDIL 

        70         80         90        100        110        120 
NINYQLALAL IVNNQEILTA CDVDFIFSNP PKSGFMTKNY SDVFFFKEEC RELDAIRANI 

       130        140        150        160        170        180 
LKLNNTYKQA LAFALMRRAM IRKMPYSRFT ISWEKVKQLR DEEYSYSKYG RRRAYHNQSF 

       190        200        210        220        230        240 
EFHFRENLNS YNQAVFNNGN IHQAYNEDVF ELLDHIQADA VYLDPPYTGT MNNYFGFYGL 

       250        260        270        280        290        300 
LDSYMSGEIR QPFDNHFMDK NQAVELFEKL IEKLKPFKYW LLSYNNVSRP NREELTAMLS 

       310        320        330 
RNGRKVTVLE TPHVYKVTGK ENKQKHTELL FLVENR 

« Hide

References

[1]"Cloning and analysis of the genes encoding the type IIS restriction-modification system HphI from Haemophilus parahaemolyticus."
Lubys A., Lubiene J., Kulakauskkas S., Stankevicius K., Timinskas A., Janulaitis A.
Nucleic Acids Res. 24:2760-2766(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 49700.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X85374 Genomic DNA. Translation: CAA59691.1.
PIRS70708.

3D structure databases

ProteinModelPortalP50193.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3661. M2.HphI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.150. 2 hits.
InterProIPR002052. DNA_methylase_N6_adenine_CS.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PRINTSPR00505. D12N6MTFRASE.
SUPFAMSSF53335. SSF53335. 1 hit.
PROSITEPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTHB_HAEPH
AccessionPrimary (citable) accession number: P50193
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries