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P50192 (MTHA_HAEPH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase HphIA

Short name=M.HphIA
EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase HphIA
M.Hphi(C)
Gene names
Name:hphIAM
Synonyms:hphIMC
OrganismHaemophilus parahaemolyticus
Taxonomic identifier735 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length372 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase protects the DNA from cleavage by the HphI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA (cytosine-5-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 372372Modification methylase HphIA
PRO_0000087884

Regions

Domain45 – 372328SAM-dependent MTase C5-type

Sites

Active site1221 By similarity

Sequences

Sequence LengthMass (Da)Tools
P50192 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 8CC8FF7A73017DDE

FASTA37242,248
        10         20         30         40         50         60 
MGFHSKNNSE YNGDFIILSS IYYWMILLYC IKFFLSKYLI YCMSLTYIDL FSGAGGFSLG 

        70         80         90        100        110        120 
FDRAGFHQLL SVEIEPHYCD TYRANFPDHQ VLQQDLTTLS DDNLLRHINH RKVDVVIGGP 

       130        140        150        160        170        180 
PCQGFSMAGK IGRTFADDPR NHLFKEFVRV VKLTQPKFFV MENVARLFTH NSGKTRAEIT 

       190        200        210        220        230        240 
EQFERLGYKV KCKVLNAADF GVPQLRSRIV FIGRKDGGEI TFPEPSHTEY NTVGDAIGHF 

       250        260        270        280        290        300 
PKLNAGENSL ILNHEAMNHS TQMLEKMSFV KNGGDRNDIP ESLRPISGDV RKYIRYHSDK 

       310        320        330        340        350        360 
PSVCVTGDMR KVFHYEQNRA LTVRELAALQ SFPDDFVFLG KKIAQQQQVG NAVPPLLAQA 

       370 
IAEAVLKMNT NE 

« Hide

References

[1]"Cloning and analysis of the genes encoding the type IIS restriction-modification system HphI from Haemophilus parahaemolyticus."
Lubys A., Lubiene J., Kulakauskkas S., Stankevicius K., Timinskas A., Janulaitis A.
Nucleic Acids Res. 24:2760-2766(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 49700.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X85374 Genomic DNA. Translation: CAA59690.1.
PIRS70707.

3D structure databases

ProteinModelPortalP50192.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3660. M1.HphI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTHA_HAEPH
AccessionPrimary (citable) accession number: P50192
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries