Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P50192

- MTHA_HAEPH

UniProt

P50192 - MTHA_HAEPH

Protein

Modification methylase HphIA

Gene

hphIAM

Organism
Haemophilus parahaemolyticus
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    This methylase protects the DNA from cleavage by the HphI endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei122 – 1221PROSITE-ProRule annotation

    GO - Molecular functioni

    1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
    2. DNA binding Source: InterPro

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3660. M1.HphI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase HphIA (EC:2.1.1.37)
    Short name:
    M.HphIA
    Alternative name(s):
    Cytosine-specific methyltransferase HphIA
    M.Hphi(C)
    Gene namesi
    Name:hphIAM
    Synonyms:hphIMC
    OrganismiHaemophilus parahaemolyticus
    Taxonomic identifieri735 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 372372Modification methylase HphIAPRO_0000087884Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP50192.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini45 – 372328SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
    Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR10629. PTHR10629. 1 hit.
    PfamiPF00145. DNA_methylase. 1 hit.
    [Graphical view]
    PRINTSiPR00105. C5METTRFRASE.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00675. dcm. 1 hit.
    PROSITEiPS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P50192-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGFHSKNNSE YNGDFIILSS IYYWMILLYC IKFFLSKYLI YCMSLTYIDL    50
    FSGAGGFSLG FDRAGFHQLL SVEIEPHYCD TYRANFPDHQ VLQQDLTTLS 100
    DDNLLRHINH RKVDVVIGGP PCQGFSMAGK IGRTFADDPR NHLFKEFVRV 150
    VKLTQPKFFV MENVARLFTH NSGKTRAEIT EQFERLGYKV KCKVLNAADF 200
    GVPQLRSRIV FIGRKDGGEI TFPEPSHTEY NTVGDAIGHF PKLNAGENSL 250
    ILNHEAMNHS TQMLEKMSFV KNGGDRNDIP ESLRPISGDV RKYIRYHSDK 300
    PSVCVTGDMR KVFHYEQNRA LTVRELAALQ SFPDDFVFLG KKIAQQQQVG 350
    NAVPPLLAQA IAEAVLKMNT NE 372
    Length:372
    Mass (Da):42,248
    Last modified:October 1, 1996 - v1
    Checksum:i8CC8FF7A73017DDE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X85374 Genomic DNA. Translation: CAA59690.1.
    PIRiS70707.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X85374 Genomic DNA. Translation: CAA59690.1 .
    PIRi S70707.

    3D structure databases

    ProteinModelPortali P50192.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3660. M1.HphI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR10629. PTHR10629. 1 hit.
    Pfami PF00145. DNA_methylase. 1 hit.
    [Graphical view ]
    PRINTSi PR00105. C5METTRFRASE.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00675. dcm. 1 hit.
    PROSITEi PS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and analysis of the genes encoding the type IIS restriction-modification system HphI from Haemophilus parahaemolyticus."
      Lubys A., Lubiene J., Kulakauskkas S., Stankevicius K., Timinskas A., Janulaitis A.
      Nucleic Acids Res. 24:2760-2766(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 49700.

    Entry informationi

    Entry nameiMTHA_HAEPH
    AccessioniPrimary (citable) accession number: P50192
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3