P50192 (MTHA_HAEPH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase HphIA
Short name=M.HphIA
EC=2.1.1.37

Alternative name(s):
Cytosine-specific methyltransferase HphIA
M.Hphi(C)

Gene names

Name:	hphIAM
Synonyms:	hphIMC

Organism

Haemophilus parahaemolyticus

Taxonomic identifier

735 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This methylase protects the DNA from cleavage by the HphI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological_process	C-5 methylation of cytosine Inferred from electronic annotation. Source: GOC DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	Modification methylase HphIA		PRO_0000087884
Sites
	Active site	122	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P50192 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 8CC8FF7A73017DDE	FASTA	372	42,248
	10 20 30 40 50 60 MGFHSKNNSE YNGDFIILSS IYYWMILLYC IKFFLSKYLI YCMSLTYIDL FSGAGGFSLG 70 80 90 100 110 120 FDRAGFHQLL SVEIEPHYCD TYRANFPDHQ VLQQDLTTLS DDNLLRHINH RKVDVVIGGP 130 140 150 160 170 180 PCQGFSMAGK IGRTFADDPR NHLFKEFVRV VKLTQPKFFV MENVARLFTH NSGKTRAEIT 190 200 210 220 230 240 EQFERLGYKV KCKVLNAADF GVPQLRSRIV FIGRKDGGEI TFPEPSHTEY NTVGDAIGHF 250 260 270 280 290 300 PKLNAGENSL ILNHEAMNHS TQMLEKMSFV KNGGDRNDIP ESLRPISGDV RKYIRYHSDK 310 320 330 340 350 360 PSVCVTGDMR KVFHYEQNRA LTVRELAALQ SFPDDFVFLG KKIAQQQQVG NAVPPLLAQA 370 IAEAVLKMNT NE

References

[1]

"Cloning and analysis of the genes encoding the type IIS restriction-modification system HphI from Haemophilus parahaemolyticus." Lubys A., Lubiene J., Kulakauskkas S., Stankevicius K., Timinskas A., Janulaitis A. Nucleic Acids Res. 24:2760-2766(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 49700.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X85374 Genomic DNA. Translation: CAA59690.1.
PIR	S70707.
3D structure databases
ProteinModelPortal	P50192.
ModBase	Search...
Protein family/group databases
REBASE	3660. M1.HphI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR018117. C5_DNA_meth_AS. IPR001525. C5_MeTfrase. [Graphical view]
PANTHER	PTHR10629. PTHR10629. 1 hit.
Pfam	PF00145. DNA_methylase. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTHA_HAEPH

Accession

Primary (citable) accession number: P50192

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program