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P50192

- MTHA_HAEPH

UniProt

P50192 - MTHA_HAEPH

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Protein

Modification methylase HphIA

Gene

hphIAM

Organism
Haemophilus parahaemolyticus
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

This methylase protects the DNA from cleavage by the HphI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei122 – 1221PROSITE-ProRule annotation

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: InterPro

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3660. M1.HphI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase HphIA (EC:2.1.1.37)
Short name:
M.HphIA
Alternative name(s):
Cytosine-specific methyltransferase HphIA
M.Hphi(C)
Gene namesi
Name:hphIAM
Synonyms:hphIMC
OrganismiHaemophilus parahaemolyticus
Taxonomic identifieri735 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 372372Modification methylase HphIAPRO_0000087884Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP50192.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini45 – 372328SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10629. PTHR10629. 1 hit.
PfamiPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50192-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGFHSKNNSE YNGDFIILSS IYYWMILLYC IKFFLSKYLI YCMSLTYIDL
60 70 80 90 100
FSGAGGFSLG FDRAGFHQLL SVEIEPHYCD TYRANFPDHQ VLQQDLTTLS
110 120 130 140 150
DDNLLRHINH RKVDVVIGGP PCQGFSMAGK IGRTFADDPR NHLFKEFVRV
160 170 180 190 200
VKLTQPKFFV MENVARLFTH NSGKTRAEIT EQFERLGYKV KCKVLNAADF
210 220 230 240 250
GVPQLRSRIV FIGRKDGGEI TFPEPSHTEY NTVGDAIGHF PKLNAGENSL
260 270 280 290 300
ILNHEAMNHS TQMLEKMSFV KNGGDRNDIP ESLRPISGDV RKYIRYHSDK
310 320 330 340 350
PSVCVTGDMR KVFHYEQNRA LTVRELAALQ SFPDDFVFLG KKIAQQQQVG
360 370
NAVPPLLAQA IAEAVLKMNT NE
Length:372
Mass (Da):42,248
Last modified:October 1, 1996 - v1
Checksum:i8CC8FF7A73017DDE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85374 Genomic DNA. Translation: CAA59690.1.
PIRiS70707.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85374 Genomic DNA. Translation: CAA59690.1 .
PIRi S70707.

3D structure databases

ProteinModelPortali P50192.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3660. M1.HphI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10629. PTHR10629. 1 hit.
Pfami PF00145. DNA_methylase. 1 hit.
[Graphical view ]
PRINTSi PR00105. C5METTRFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00675. dcm. 1 hit.
PROSITEi PS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and analysis of the genes encoding the type IIS restriction-modification system HphI from Haemophilus parahaemolyticus."
    Lubys A., Lubiene J., Kulakauskkas S., Stankevicius K., Timinskas A., Janulaitis A.
    Nucleic Acids Res. 24:2760-2766(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 49700.

Entry informationi

Entry nameiMTHA_HAEPH
AccessioniPrimary (citable) accession number: P50192
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 1, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3