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P50191

- T2H1_HAEPH

UniProt

P50191 - T2H1_HAEPH

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Protein
Type-2 restriction enzyme HphI
Gene
hphIR
Organism
Haemophilus parahaemolyticus
Status
Reviewed - Annotation score: 2 out of 5 - Protein predictedi

Functioni

Recognizes the double-stranded sequences GGTGA and TCACC and cleaves respectively 13 bases after G-1 and 7 bases before T-1, leaving a single 3' protruding nucleotide.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi1160. HphI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme HphI (EC:3.1.21.4)
Short name:
R.HphI
Alternative name(s):
Endonuclease HphI
Type II restriction enzyme HphI
Gene namesi
Name:hphIR
OrganismiHaemophilus parahaemolyticus
Taxonomic identifieri735 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 379379Type-2 restriction enzyme HphI
PRO_0000077326Add
BLAST

Family & Domainsi

Family and domain databases

InterProiIPR003615. HNH_nuc.
[Graphical view]
PfamiPF13391. HNH_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50191-1 [UniParc]FASTAAdd to Basket

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MQIYETYWEI TNEYGYNTER FVETLKICVE YIDEIKLVNP NYTETDYSSV    50
IYNELQMRLQ SSPILQSTRK GFEGKPKNET SIRKSINQLV KSGFINPFLT 100
GYHSLAKEYL QTKVNKKRNF LFSRIVYESS NFSYAITDKP DIKVRHINFL 150
VNTLIENFEG KLSKNEIIAL MLMDLRTYNG NYYPIDELRN FIRLNQNYIS 200
EFKERKYNQI TYLWGLLSKL DEIYQKDEFI CLEEDKKRVF GDLEDTQYLR 250
KRDPYLHRLY KHQLQEESAE YCGGIKCMLE KLAYPVLIAS HIKPFIQSDD 300
NEAYDPNNGL LLSRTLDSLF DLKYISFDDN GNMLKSARLS DDVWQYWRNI 350
KLDSVLLNEK RKQYLKFHRE LMEKEDSKN 379
Length:379
Mass (Da):45,321
Last modified:October 1, 1996 - v1
Checksum:i771488C513C74E34
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X85374 Genomic DNA. Translation: CAA59692.1.
PIRiS70709.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X85374 Genomic DNA. Translation: CAA59692.1 .
PIRi S70709.

3D structure databases

ModBasei Search...

Protein family/group databases

REBASEi 1160. HphI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR003615. HNH_nuc.
[Graphical view ]
Pfami PF13391. HNH_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and analysis of the genes encoding the type IIS restriction-modification system HphI from Haemophilus parahaemolyticus."
    Lubys A., Lubiene J., Kulakauskkas S., Stankevicius K., Timinskas A., Janulaitis A.
    Nucleic Acids Res. 24:2760-2766(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 49700.

Entry informationi

Entry nameiT2H1_HAEPH
AccessioniPrimary (citable) accession number: P50191
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

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