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P50191 (T2H1_HAEPH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme HphI

Short name=R.HphI
EC=3.1.21.4
Alternative name(s):
Endonuclease HphI
Type II restriction enzyme HphI
Gene names
Name:hphIR
OrganismHaemophilus parahaemolyticus
Taxonomic identifier735 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length379 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded sequences GGTGA and TCACC and cleaves respectively 13 bases after G-1 and 7 bases before T-1, leaving a single 3' protruding nucleotide.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionType II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 379379Type-2 restriction enzyme HphI
PRO_0000077326

Sequences

Sequence LengthMass (Da)Tools
P50191 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 771488C513C74E34

FASTA37945,321
        10         20         30         40         50         60 
MQIYETYWEI TNEYGYNTER FVETLKICVE YIDEIKLVNP NYTETDYSSV IYNELQMRLQ 

        70         80         90        100        110        120 
SSPILQSTRK GFEGKPKNET SIRKSINQLV KSGFINPFLT GYHSLAKEYL QTKVNKKRNF 

       130        140        150        160        170        180 
LFSRIVYESS NFSYAITDKP DIKVRHINFL VNTLIENFEG KLSKNEIIAL MLMDLRTYNG 

       190        200        210        220        230        240 
NYYPIDELRN FIRLNQNYIS EFKERKYNQI TYLWGLLSKL DEIYQKDEFI CLEEDKKRVF 

       250        260        270        280        290        300 
GDLEDTQYLR KRDPYLHRLY KHQLQEESAE YCGGIKCMLE KLAYPVLIAS HIKPFIQSDD 

       310        320        330        340        350        360 
NEAYDPNNGL LLSRTLDSLF DLKYISFDDN GNMLKSARLS DDVWQYWRNI KLDSVLLNEK 

       370 
RKQYLKFHRE LMEKEDSKN 

« Hide

References

[1]"Cloning and analysis of the genes encoding the type IIS restriction-modification system HphI from Haemophilus parahaemolyticus."
Lubys A., Lubiene J., Kulakauskkas S., Stankevicius K., Timinskas A., Janulaitis A.
Nucleic Acids Res. 24:2760-2766(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 49700.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X85374 Genomic DNA. Translation: CAA59692.1.
PIRS70709.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE1160. HphI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR003615. HNH_nuc.
[Graphical view]
PfamPF13391. HNH_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT2H1_HAEPH
AccessionPrimary (citable) accession number: P50191
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries