P50191 (T2H1_HAEPH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-2 restriction enzyme HphI
Short name=R.HphI
EC=3.1.21.4

Alternative name(s):
Endonuclease HphI
Type II restriction enzyme HphI

Gene names

Name:

hphIR

Organism

Haemophilus parahaemolyticus

Taxonomic identifier

735 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	379 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Recognizes the double-stranded sequences GGTGA and TCACC and cleaves respectively 13 bases after G-1 and 7 bases before T-1, leaving a single 3' protruding nucleotide.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 379	379	Type-2 restriction enzyme HphI		PRO_0000077326

Sequences

	Sequence		Length	Mass (Da)
	P50191 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 771488C513C74E34	FASTA	379	45,321
	10 20 30 40 50 60 MQIYETYWEI TNEYGYNTER FVETLKICVE YIDEIKLVNP NYTETDYSSV IYNELQMRLQ 70 80 90 100 110 120 SSPILQSTRK GFEGKPKNET SIRKSINQLV KSGFINPFLT GYHSLAKEYL QTKVNKKRNF 130 140 150 160 170 180 LFSRIVYESS NFSYAITDKP DIKVRHINFL VNTLIENFEG KLSKNEIIAL MLMDLRTYNG 190 200 210 220 230 240 NYYPIDELRN FIRLNQNYIS EFKERKYNQI TYLWGLLSKL DEIYQKDEFI CLEEDKKRVF 250 260 270 280 290 300 GDLEDTQYLR KRDPYLHRLY KHQLQEESAE YCGGIKCMLE KLAYPVLIAS HIKPFIQSDD 310 320 330 340 350 360 NEAYDPNNGL LLSRTLDSLF DLKYISFDDN GNMLKSARLS DDVWQYWRNI KLDSVLLNEK 370 RKQYLKFHRE LMEKEDSKN

References

[1]

"Cloning and analysis of the genes encoding the type IIS restriction-modification system HphI from Haemophilus parahaemolyticus." Lubys A., Lubiene J., Kulakauskkas S., Stankevicius K., Timinskas A., Janulaitis A. Nucleic Acids Res. 24:2760-2766(1996) [PubMed: 8759008] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 49700.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X85374 Genomic DNA. Translation: CAA59692.1.
PIR	S70709.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	1160. HphI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

T2H1_HAEPH

Accession

Primary (citable) accession number: P50191

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 31, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program