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P50191

- T2H1_HAEPH

UniProt

P50191 - T2H1_HAEPH

Protein

Type-2 restriction enzyme HphI

Gene

hphIR

Organism
Haemophilus parahaemolyticus
Status
Reviewed - Annotation score: 2 out of 5- Protein predictedi
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    • History
      Entry version 41 (16 Apr 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequences GGTGA and TCACC and cleaves respectively 13 bases after G-1 and 7 bases before T-1, leaving a single 3' protruding nucleotide.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    GO - Molecular functioni

    1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Protein family/group databases

    REBASEi1160. HphI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme HphI (EC:3.1.21.4)
    Short name:
    R.HphI
    Alternative name(s):
    Endonuclease HphI
    Type II restriction enzyme HphI
    Gene namesi
    Name:hphIR
    OrganismiHaemophilus parahaemolyticus
    Taxonomic identifieri735 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 379379Type-2 restriction enzyme HphIPRO_0000077326Add
    BLAST

    Family & Domainsi

    Family and domain databases

    InterProiIPR003615. HNH_nuc.
    [Graphical view]
    PfamiPF13391. HNH_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P50191-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQIYETYWEI TNEYGYNTER FVETLKICVE YIDEIKLVNP NYTETDYSSV    50
    IYNELQMRLQ SSPILQSTRK GFEGKPKNET SIRKSINQLV KSGFINPFLT 100
    GYHSLAKEYL QTKVNKKRNF LFSRIVYESS NFSYAITDKP DIKVRHINFL 150
    VNTLIENFEG KLSKNEIIAL MLMDLRTYNG NYYPIDELRN FIRLNQNYIS 200
    EFKERKYNQI TYLWGLLSKL DEIYQKDEFI CLEEDKKRVF GDLEDTQYLR 250
    KRDPYLHRLY KHQLQEESAE YCGGIKCMLE KLAYPVLIAS HIKPFIQSDD 300
    NEAYDPNNGL LLSRTLDSLF DLKYISFDDN GNMLKSARLS DDVWQYWRNI 350
    KLDSVLLNEK RKQYLKFHRE LMEKEDSKN 379
    Length:379
    Mass (Da):45,321
    Last modified:October 1, 1996 - v1
    Checksum:i771488C513C74E34
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X85374 Genomic DNA. Translation: CAA59692.1.
    PIRiS70709.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X85374 Genomic DNA. Translation: CAA59692.1 .
    PIRi S70709.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 1160. HphI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR003615. HNH_nuc.
    [Graphical view ]
    Pfami PF13391. HNH_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and analysis of the genes encoding the type IIS restriction-modification system HphI from Haemophilus parahaemolyticus."
      Lubys A., Lubiene J., Kulakauskkas S., Stankevicius K., Timinskas A., Janulaitis A.
      Nucleic Acids Res. 24:2760-2766(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 49700.

    Entry informationi

    Entry nameiT2H1_HAEPH
    AccessioniPrimary (citable) accession number: P50191
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: April 16, 2014
    This is version 41 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3