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P50190

- MTM1_MICAM

UniProt

P50190 - MTM1_MICAM

Protein

Modification methylase MamI

Gene

mamIM

Organism
Microbacterium ammoniaphilum
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 51 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    This methylase recognizes the double-stranded sequence GATNNNNATC, causes specific methylation on A-? on both strands, and protects the DNA from cleavage by the MamI endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. N-methyltransferase activity Source: InterPro
    3. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3439. M.MamI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase MamI (EC:2.1.1.72)
    Short name:
    M.MamI
    Alternative name(s):
    Adenine-specific methyltransferase MamI
    Gene namesi
    Name:mamIM
    OrganismiMicrobacterium ammoniaphilum
    Taxonomic identifieri42460 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrobacteriaceaeMicrobacterium

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 362362Modification methylase MamIPRO_0000087975Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP50190.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the N(4)/N(6)-methyltransferase family.Curated

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR003356. DNA_methylase_A-5.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR002296. N12N6_MeTrfase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF02384. N6_Mtase. 1 hit.
    [Graphical view]
    PRINTSiPR00507. N12N6MTFRASE.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS00092. N6_MTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P50190-1 [UniParc]FASTAAdd to Basket

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    MRPLRHAVGS STVTLETDLT LFPEDLHAPL LGSAGNATVD ELALAARFDG    50
    LHQLLYTRGG VRPTNAAIEE VGKLLLLRLW LSRDDEASVD GVGLRALFDG 100
    AVPDESVVEV TKKAFTQVLT VDRMSLRAVD GSSRPLWPYD EPFRLAEPTV 150
    LQSALALVNE ILGGGTRVAD PLGTAFDAFL SGRYDHSGGL GTYLTPSSVA 200
    RMMAEVVLDL LSSDALADVR APIIADPFCG TGRFLVAAFD AAEERHENVD 250
    LAGLLDGGLV GADQSTTAIA KSGLNLLLYG AQQPEVYAVA DSMTDPGLDR 300
    LRGTLAAVLT NPPFGGGKYD DALGIDRTRE LFPSVRPNRP MDPRLLDSRC 350
    LSNFCDPGES LG 362
    Length:362
    Mass (Da):38,501
    Last modified:October 1, 1996 - v1
    Checksum:i85CE1ADFB54ADC84
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X79027 Genomic DNA. Translation: CAA55646.1.
    PIRiT45131.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X79027 Genomic DNA. Translation: CAA55646.1 .
    PIRi T45131.

    3D structure databases

    ProteinModelPortali P50190.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3439. M.MamI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR003356. DNA_methylase_A-5.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR002296. N12N6_MeTrfase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF02384. N6_Mtase. 1 hit.
    [Graphical view ]
    PRINTSi PR00507. N12N6MTFRASE.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS00092. N6_MTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of the MamI restriction-modification system from Microbacterium ammoniaphilum in Escherichia coli."
      Striebel H.-M., Seeber S., Jarsch M., Kessler C.
      Gene 172:41-46(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 15354 / NCIB 10335.

    Entry informationi

    Entry nameiMTM1_MICAM
    AccessioniPrimary (citable) accession number: P50190
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 51 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3