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Protein

Modification methylase MamI

Gene

mamIM

Organism
Microbacterium ammoniaphilum
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GATNNNNATC, causes specific methylation on A-? on both strands, and protects the DNA from cleavage by the MamI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. N-methyltransferase activity Source: InterPro
  3. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3439. M.MamI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase MamI (EC:2.1.1.72)
Short name:
M.MamI
Alternative name(s):
Adenine-specific methyltransferase MamI
Gene namesi
Name:mamIM
OrganismiMicrobacterium ammoniaphilum
Taxonomic identifieri42460 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrobacteriaceaeMicrobacterium

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 362362Modification methylase MamIPRO_0000087975Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP50190.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR003356. DNA_methylase_A-5.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02384. N6_Mtase. 1 hit.
[Graphical view]
PRINTSiPR00507. N12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50190-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRPLRHAVGS STVTLETDLT LFPEDLHAPL LGSAGNATVD ELALAARFDG
60 70 80 90 100
LHQLLYTRGG VRPTNAAIEE VGKLLLLRLW LSRDDEASVD GVGLRALFDG
110 120 130 140 150
AVPDESVVEV TKKAFTQVLT VDRMSLRAVD GSSRPLWPYD EPFRLAEPTV
160 170 180 190 200
LQSALALVNE ILGGGTRVAD PLGTAFDAFL SGRYDHSGGL GTYLTPSSVA
210 220 230 240 250
RMMAEVVLDL LSSDALADVR APIIADPFCG TGRFLVAAFD AAEERHENVD
260 270 280 290 300
LAGLLDGGLV GADQSTTAIA KSGLNLLLYG AQQPEVYAVA DSMTDPGLDR
310 320 330 340 350
LRGTLAAVLT NPPFGGGKYD DALGIDRTRE LFPSVRPNRP MDPRLLDSRC
360
LSNFCDPGES LG
Length:362
Mass (Da):38,501
Last modified:October 1, 1996 - v1
Checksum:i85CE1ADFB54ADC84
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79027 Genomic DNA. Translation: CAA55646.1.
PIRiT45131.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79027 Genomic DNA. Translation: CAA55646.1.
PIRiT45131.

3D structure databases

ProteinModelPortaliP50190.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi3439. M.MamI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR003356. DNA_methylase_A-5.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02384. N6_Mtase. 1 hit.
[Graphical view]
PRINTSiPR00507. N12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and characterization of the MamI restriction-modification system from Microbacterium ammoniaphilum in Escherichia coli."
    Striebel H.-M., Seeber S., Jarsch M., Kessler C.
    Gene 172:41-46(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 15354 / NCIB 10335.

Entry informationi

Entry nameiMTM1_MICAM
AccessioniPrimary (citable) accession number: P50190
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: January 7, 2015
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.