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P50190 (MTM1_MICAM) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Modification methylase MamI

Short name=M.MamI
EC=2.1.1.72
Alternative name(s):
Adenine-specific methyltransferase MamI
Gene names
Name:mamIM
OrganismMicrobacterium ammoniaphilum
Taxonomic identifier42460 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrobacteriaceaeMicrobacterium

Protein attributes

Sequence length362 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence GATNNNNATC, causes specific methylation on A-? on both strands, and protects the DNA from cleavage by the MamI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 362362Modification methylase MamI
PRO_0000087975

Sequences

Sequence LengthMass (Da)Tools
P50190 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 85CE1ADFB54ADC84

FASTA36238,501
        10         20         30         40         50         60 
MRPLRHAVGS STVTLETDLT LFPEDLHAPL LGSAGNATVD ELALAARFDG LHQLLYTRGG 

        70         80         90        100        110        120 
VRPTNAAIEE VGKLLLLRLW LSRDDEASVD GVGLRALFDG AVPDESVVEV TKKAFTQVLT 

       130        140        150        160        170        180 
VDRMSLRAVD GSSRPLWPYD EPFRLAEPTV LQSALALVNE ILGGGTRVAD PLGTAFDAFL 

       190        200        210        220        230        240 
SGRYDHSGGL GTYLTPSSVA RMMAEVVLDL LSSDALADVR APIIADPFCG TGRFLVAAFD 

       250        260        270        280        290        300 
AAEERHENVD LAGLLDGGLV GADQSTTAIA KSGLNLLLYG AQQPEVYAVA DSMTDPGLDR 

       310        320        330        340        350        360 
LRGTLAAVLT NPPFGGGKYD DALGIDRTRE LFPSVRPNRP MDPRLLDSRC LSNFCDPGES 


LG 

« Hide

References

[1]"Cloning and characterization of the MamI restriction-modification system from Microbacterium ammoniaphilum in Escherichia coli."
Striebel H.-M., Seeber S., Jarsch M., Kessler C.
Gene 172:41-46(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 15354 / NCIB 10335.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X79027 Genomic DNA. Translation: CAA55646.1.
PIRT45131.

3D structure databases

ProteinModelPortalP50190.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3439. M.MamI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR003356. DNA_methylase_A-5.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
[Graphical view]
PfamPF02384. N6_Mtase. 1 hit.
[Graphical view]
PRINTSPR00507. N12N6MTFRASE.
PROSITEPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTM1_MICAM
AccessionPrimary (citable) accession number: P50190
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries