Reviewed, UniProtKB/Swiss-Prot P50190 (MTM1_MICAM)

Names and origin

Protein names

Recommended name:
    Modification methylase MamI
      Short name=M.MamI
    EC=2.1.1.72
Alternative name(s):
    Adenine-specific methyltransferase MamI

Gene names

Name:

mamIM

Organism

Microbacterium ammoniaphilum

Taxonomic identifier

42460 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Microbacteriaceae › Microbacterium

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GATNNNNATC, causes specific methylation on A-? on both strands, and protects the DNA from cleavage by the MamI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW methylation Inferred from electronic annotation. Source: InterPro
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 362	362	Modification methylase MamI		PRO_0000087975

Sequences

	Sequence		Length	Mass (Da)
	P50190-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 85CE1ADFB54ADC84	FASTA	362	38,501
	10 20 30 40 50 60 MRPLRHAVGS STVTLETDLT LFPEDLHAPL LGSAGNATVD ELALAARFDG LHQLLYTRGG 70 80 90 100 110 120 VRPTNAAIEE VGKLLLLRLW LSRDDEASVD GVGLRALFDG AVPDESVVEV TKKAFTQVLT 130 140 150 160 170 180 VDRMSLRAVD GSSRPLWPYD EPFRLAEPTV LQSALALVNE ILGGGTRVAD PLGTAFDAFL 190 200 210 220 230 240 SGRYDHSGGL GTYLTPSSVA RMMAEVVLDL LSSDALADVR APIIADPFCG TGRFLVAAFD 250 260 270 280 290 300 AAEERHENVD LAGLLDGGLV GADQSTTAIA KSGLNLLLYG AQQPEVYAVA DSMTDPGLDR 310 320 330 340 350 360 LRGTLAAVLT NPPFGGGKYD DALGIDRTRE LFPSVRPNRP MDPRLLDSRC LSNFCDPGES LG

References

[1]

"Cloning and characterization of the MamI restriction-modification system from Microbacterium ammoniaphilum in Escherichia coli." Striebel H.-M., Seeber S., Jarsch M., Kessler C. Gene 172:41-46(1996) [PubMed: 8654988] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 15354 / NCIB 10335.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X79027 Genomic DNA. Translation: CAA55646.1.
PIR	T45131.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	3439. M.MamI.
Enzyme and pathway databases
BRENDA	2.1.1.72. 292785.
Family and domain databases
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR002296. N12N6_MeTrfase. [Graphical view]
PRINTS	PR00507. N12N6MTFRASE.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTM1_MICAM

Accession

Primary (citable) accession number: P50190

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	September 22, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)