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P50189

- T2M1_MICAM

UniProt

P50189 - T2M1_MICAM

Protein

Type-2 restriction enzyme MamI

Gene

mamIR

Organism
Microbacterium ammoniaphilum
Status
Reviewed - Annotation score: 2 out of 5- Protein predictedi
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    • History
      Entry version 43 (16 Apr 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequence GATNNNNATC and cleaves after N-5.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Protein family/group databases

    REBASEi1200. MamI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme MamI (EC:3.1.21.4)
    Short name:
    R.MamI
    Alternative name(s):
    Endonuclease MamI
    Type II restriction enzyme MamI
    Gene namesi
    Name:mamIR
    OrganismiMicrobacterium ammoniaphilum
    Taxonomic identifieri42460 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrobacteriaceaeMicrobacterium

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 308308Type-2 restriction enzyme MamIPRO_0000077329Add
    BLAST

    Family & Domainsi

    Family and domain databases

    InterProiIPR019067. Restrct_endonuc_II_MamI.
    [Graphical view]
    PfamiPF09567. RE_MamI. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P50189-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQNAVSQAIS QGIHVRREIL GSLTYEQRVF LLEDLFVDLF GHQHVMLQRW    50
    AALTGQSAQV DTGYIAQFVA SIVLGEPGQG FRGKGDDLAD GSEVKSAANI 100
    SGVDRPRWNH NLGSLDDDEH RRSRGLPTAG EEYLGVPYMF YLLVDRPHGV 150
    SDPAPIRIRA WCIDAQEDGD WRDLFETFLT SRRGRTYNFQ LHPPVGYDDD 200
    VVVNTLGNLD FSNVLVFDAR LSLADRDRPE IDWHVPLPTQ VIPVTGRTRA 250
    LRYGGRGARP TRLTNTADIV LGTNDLGALF PGVLAPRDSY DLATVSEIET 300
    EAEVEEYS 308
    Length:308
    Mass (Da):34,293
    Last modified:October 1, 1996 - v1
    Checksum:i53057207FBF1BB92
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X79027 Genomic DNA. Translation: CAA55648.1.
    PIRiT45133.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X79027 Genomic DNA. Translation: CAA55648.1 .
    PIRi T45133.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 1200. MamI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR019067. Restrct_endonuc_II_MamI.
    [Graphical view ]
    Pfami PF09567. RE_MamI. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of the MamI restriction-modification system from Microbacterium ammoniaphilum in Escherichia coli."
      Striebel H.-M., Seeber S., Jarsch M., Kessler C.
      Gene 172:41-46(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 15354 / NCIB 10335.

    Entry informationi

    Entry nameiT2M1_MICAM
    AccessioniPrimary (citable) accession number: P50189
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: April 16, 2014
    This is version 43 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3