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Protein

Type-2 restriction enzyme MamI

Gene

mamIR

Organism
Microbacterium ammoniaphilum
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein predictedi

Functioni

Recognizes the double-stranded sequence GATNNNNATC and cleaves after N-5.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi1200. MamI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme MamI (EC:3.1.21.4)
Short name:
R.MamI
Alternative name(s):
Endonuclease MamI
Type II restriction enzyme MamI
Gene namesi
Name:mamIR
OrganismiMicrobacterium ammoniaphilum
Taxonomic identifieri42460 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrobacteriaceaeMicrobacterium

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 308308Type-2 restriction enzyme MamIPRO_0000077329Add
BLAST

Family & Domainsi

Family and domain databases

InterProiIPR019067. Restrct_endonuc_II_MamI.
[Graphical view]
PfamiPF09567. RE_MamI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50189-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQNAVSQAIS QGIHVRREIL GSLTYEQRVF LLEDLFVDLF GHQHVMLQRW
60 70 80 90 100
AALTGQSAQV DTGYIAQFVA SIVLGEPGQG FRGKGDDLAD GSEVKSAANI
110 120 130 140 150
SGVDRPRWNH NLGSLDDDEH RRSRGLPTAG EEYLGVPYMF YLLVDRPHGV
160 170 180 190 200
SDPAPIRIRA WCIDAQEDGD WRDLFETFLT SRRGRTYNFQ LHPPVGYDDD
210 220 230 240 250
VVVNTLGNLD FSNVLVFDAR LSLADRDRPE IDWHVPLPTQ VIPVTGRTRA
260 270 280 290 300
LRYGGRGARP TRLTNTADIV LGTNDLGALF PGVLAPRDSY DLATVSEIET

EAEVEEYS
Length:308
Mass (Da):34,293
Last modified:October 1, 1996 - v1
Checksum:i53057207FBF1BB92
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79027 Genomic DNA. Translation: CAA55648.1.
PIRiT45133.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79027 Genomic DNA. Translation: CAA55648.1.
PIRiT45133.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi1200. MamI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR019067. Restrct_endonuc_II_MamI.
[Graphical view]
PfamiPF09567. RE_MamI. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and characterization of the MamI restriction-modification system from Microbacterium ammoniaphilum in Escherichia coli."
    Striebel H.-M., Seeber S., Jarsch M., Kessler C.
    Gene 172:41-46(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 15354 / NCIB 10335.

Entry informationi

Entry nameiT2M1_MICAM
AccessioniPrimary (citable) accession number: P50189
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.