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P50189 (T2M1_MICAM) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme MamI

Short name=R.MamI
EC=3.1.21.4
Alternative name(s):
Endonuclease MamI
Type II restriction enzyme MamI
Gene names
Name:mamIR
OrganismMicrobacterium ammoniaphilum
Taxonomic identifier42460 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrobacteriaceaeMicrobacterium

Protein attributes

Sequence length308 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded sequence GATNNNNATC and cleaves after N-5.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

Type II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 308308Type-2 restriction enzyme MamI
PRO_0000077329

Sequences

Sequence LengthMass (Da)Tools
P50189 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 53057207FBF1BB92

FASTA30834,293
        10         20         30         40         50         60 
MQNAVSQAIS QGIHVRREIL GSLTYEQRVF LLEDLFVDLF GHQHVMLQRW AALTGQSAQV 

        70         80         90        100        110        120 
DTGYIAQFVA SIVLGEPGQG FRGKGDDLAD GSEVKSAANI SGVDRPRWNH NLGSLDDDEH 

       130        140        150        160        170        180 
RRSRGLPTAG EEYLGVPYMF YLLVDRPHGV SDPAPIRIRA WCIDAQEDGD WRDLFETFLT 

       190        200        210        220        230        240 
SRRGRTYNFQ LHPPVGYDDD VVVNTLGNLD FSNVLVFDAR LSLADRDRPE IDWHVPLPTQ 

       250        260        270        280        290        300 
VIPVTGRTRA LRYGGRGARP TRLTNTADIV LGTNDLGALF PGVLAPRDSY DLATVSEIET 


EAEVEEYS 

« Hide

References

[1]"Cloning and characterization of the MamI restriction-modification system from Microbacterium ammoniaphilum in Escherichia coli."
Striebel H.-M., Seeber S., Jarsch M., Kessler C.
Gene 172:41-46(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 15354 / NCIB 10335.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X79027 Genomic DNA. Translation: CAA55648.1.
PIRT45133.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE1200. MamI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR019067. Restrct_endonuc_II_MamI.
[Graphical view]
PfamPF09567. RE_MamI. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT2M1_MICAM
AccessionPrimary (citable) accession number: P50189
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries