Reviewed, UniProtKB/Swiss-Prot P50189 (T2M1_MICAM)

Names and origin

Protein names

Recommended name:
    Type-2 restriction enzyme MamI
      Short name=R.MamI
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme MamI
    Endonuclease MamI

Gene names

Name:

mamIR

Organism

Microbacterium ammoniaphilum

Taxonomic identifier

42460 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Microbacteriaceae › Microbacterium

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Protein existence	Predicted.

General annotation (Comments)

Function	Recognizes the double-stranded sequence GATNNNNATC and cleaves after N-5.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	Type-2 restriction enzyme MamI		PRO_0000077329

Sequences

	Sequence		Length	Mass (Da)
	P50189-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 53057207FBF1BB92	FASTA	308	34,293
	10 20 30 40 50 60 MQNAVSQAIS QGIHVRREIL GSLTYEQRVF LLEDLFVDLF GHQHVMLQRW AALTGQSAQV 70 80 90 100 110 120 DTGYIAQFVA SIVLGEPGQG FRGKGDDLAD GSEVKSAANI SGVDRPRWNH NLGSLDDDEH 130 140 150 160 170 180 RRSRGLPTAG EEYLGVPYMF YLLVDRPHGV SDPAPIRIRA WCIDAQEDGD WRDLFETFLT 190 200 210 220 230 240 SRRGRTYNFQ LHPPVGYDDD VVVNTLGNLD FSNVLVFDAR LSLADRDRPE IDWHVPLPTQ 250 260 270 280 290 300 VIPVTGRTRA LRYGGRGARP TRLTNTADIV LGTNDLGALF PGVLAPRDSY DLATVSEIET EAEVEEYS

References

[1]

"Cloning and characterization of the MamI restriction-modification system from Microbacterium ammoniaphilum in Escherichia coli." Striebel H.-M., Seeber S., Jarsch M., Kessler C. Gene 172:41-46(1996) [PubMed: 8654988] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 15354 / NCIB 10335.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X79027 Genomic DNA. Translation: CAA55648.1.
PIR	T45133.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	1200. MamI.
Enzyme and pathway databases
BRENDA	3.1.21.4. 292785.
Family and domain databases
InterPro	IPR019067. Restrict_endonuc_II_MamI. [Graphical view]
Pfam	PF09567. RE_MamI. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T2M1_MICAM

Accession

Primary (citable) accession number: P50189

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 3, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)