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Reviewed, UniProtKB/Swiss-Prot P50188 (MTN1_NOCAE)

Last modified March 3, 2009. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Modification methylase NaeI
      Short name=M.NaeI
    EC=2.1.1.37
Alternative name(s):
    Cytosine-specific methyltransferase NaeI
Gene names
Name: naeIM
OrganismNocardia aerocolonigenes (Lechevalieria aerocolonigenes)
Taxonomic identifier68170 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesPseudonocardineaeActinosynnemataceaeLechevalieria

Protein attributes

Sequence length413 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence GCCGGC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the NaeI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sequence similarities

Belongs to the C5-methyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 413413Modification methylase NaeI
PRO_0000087903

Sites

Active site781 By similarity

Sequences

Sequence LengthMass (Da)Tools
P50188-1 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 649CF1C2B97EFD28

FASTA41345,180
        10         20         30         40         50         60 
MQSLEVVEIC AGAGGQALGL EKAGFSHRLA VELDVNAAAT LRKNLKSDVV ITGDVADPSV 

        70         80         90        100        110        120 
LNPMEHLGVS LLAGGVPCPP FSIAGKQLGA DDMRDLFAWA VELCDVMKPR ALMLENVRGL 

       130        140        150        160        170        180 
SMPRFAGYRQ HVLDRLNDMG YVAEWRLLHA SDFGVPQLRP RFVLVALQNK FAPYFTWPEP 

       190        200        210        220        230        240 
TGAAPTVGET LKDLMAADGW EGAEEWAAQA NDIAPTIVGG SKKHGGADLG PTRAKRAWAE 

       250        260        270        280        290        300 
LGVDAMGVAD APPQPGDKFK VGPKLTCEMV ARIQGWRDGE WIFEGRKTSR YRQIGNAFPP 

       310        320        330        340        350        360 
PVAEAIGKRI RAALNMEGEG RDRAVDSDHN PLYRALKESG DFMTHRQLER AVGRPIEAYE 

       370        380        390        400        410 
LERTISDLGR DFEVETKDGA SAMAYKLGPF KAFTGQEGHL RHEMFVRHRT KIS 

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References

[1]"Cloning and expression of the NaeI restriction endonuclease-encoding gene and sequence analysis of the NaeI restriction-modification system."
Taron C.H., van Cott E.M., Wilson G.G., Moran L.S., Slatko B.E., Hornstra L.J., Benner J.S., Kucera R.B., Guthrie E.P.
Gene 155:19-25(1995) [PubMed: 7698663] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 23870 / BCRC 13661 / DSM 40034 / IFO 13195.

Cross-references

Sequence databases

U09581 Genomic DNA. Translation: AAC43325.1.

3D structure databases

ModBaseSearch...

Protein family/group databases

REBASE3452. M.NaeI.

Enzyme and pathway databases

BRENDA2.1.1.37. 96201.

Family and domain databases

InterProIPR001525. C5_DNA_meth.
IPR018117. C5_DNA_meth_AS.
[Graphical view]
PANTHERPTHR10629. C5_DNA_meth. 1 hit.
PfamPF00145. DNA_methylase. 2 hits.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTN1_NOCAE
AccessionPrimary (citable) accession number: P50188
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 3, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents