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Protein

Modification methylase NaeI

Gene

naeIM

Organism
Lechevalieria aerocolonigenes (Nocardia aerocolonigenes) (Saccharothrix aerocolonigenes)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GCCGGC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the NaeI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei78PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processRestriction system
LigandS-adenosyl-L-methionine

Protein family/group databases

REBASEi3452 M.NaeI

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase NaeI (EC:2.1.1.37)
Short name:
M.NaeI
Alternative name(s):
Cytosine-specific methyltransferase NaeI
Gene namesi
Name:naeIM
OrganismiLechevalieria aerocolonigenes (Nocardia aerocolonigenes) (Saccharothrix aerocolonigenes)
Taxonomic identifieri68170 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaPseudonocardialesPseudonocardiaceaeLechevalieria

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000879031 – 413Modification methylase NaeIAdd BLAST413

Structurei

3D structure databases

ProteinModelPortaliP50188
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 317SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd BLAST314

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation

Family and domain databases

InterProiView protein in InterPro
IPR018117 C5_DNA_meth_AS
IPR001525 C5_MeTfrase
IPR031303 C5_meth_CS
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF00145 DNA_methylase, 1 hit
PRINTSiPR00105 C5METTRFRASE
SUPFAMiSSF53335 SSF53335, 2 hits
TIGRFAMsiTIGR00675 dcm, 1 hit
PROSITEiView protein in PROSITE
PS00094 C5_MTASE_1, 1 hit
PS00095 C5_MTASE_2, 1 hit
PS51679 SAM_MT_C5, 1 hit

Sequencei

Sequence statusi: Complete.

P50188-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQSLEVVEIC AGAGGQALGL EKAGFSHRLA VELDVNAAAT LRKNLKSDVV
60 70 80 90 100
ITGDVADPSV LNPMEHLGVS LLAGGVPCPP FSIAGKQLGA DDMRDLFAWA
110 120 130 140 150
VELCDVMKPR ALMLENVRGL SMPRFAGYRQ HVLDRLNDMG YVAEWRLLHA
160 170 180 190 200
SDFGVPQLRP RFVLVALQNK FAPYFTWPEP TGAAPTVGET LKDLMAADGW
210 220 230 240 250
EGAEEWAAQA NDIAPTIVGG SKKHGGADLG PTRAKRAWAE LGVDAMGVAD
260 270 280 290 300
APPQPGDKFK VGPKLTCEMV ARIQGWRDGE WIFEGRKTSR YRQIGNAFPP
310 320 330 340 350
PVAEAIGKRI RAALNMEGEG RDRAVDSDHN PLYRALKESG DFMTHRQLER
360 370 380 390 400
AVGRPIEAYE LERTISDLGR DFEVETKDGA SAMAYKLGPF KAFTGQEGHL
410
RHEMFVRHRT KIS
Length:413
Mass (Da):45,180
Last modified:October 1, 1996 - v1
Checksum:i649CF1C2B97EFD28
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09581 Genomic DNA Translation: AAC43325.1
RefSeqiWP_030468108.1, NZ_JOFI01000013.1

Similar proteinsi

Entry informationi

Entry nameiMTN1_NOCAE
AccessioniPrimary (citable) accession number: P50188
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 28, 2018
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health