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P50188

- MTN1_NOCAE

UniProt

P50188 - MTN1_NOCAE

Protein

Modification methylase NaeI

Gene

naeIM

Organism
Lechevalieria aerocolonigenes (Nocardia aerocolonigenes) (Saccharothrix aerocolonigenes)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 59 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    This methylase recognizes the double-stranded sequence GCCGGC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the NaeI endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei78 – 781PROSITE-ProRule annotation

    GO - Molecular functioni

    1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
    2. DNA binding Source: InterPro

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3452. M.NaeI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase NaeI (EC:2.1.1.37)
    Short name:
    M.NaeI
    Alternative name(s):
    Cytosine-specific methyltransferase NaeI
    Gene namesi
    Name:naeIM
    OrganismiLechevalieria aerocolonigenes (Nocardia aerocolonigenes) (Saccharothrix aerocolonigenes)
    Taxonomic identifieri68170 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesPseudonocardineaePseudonocardiaceaeLechevalieria

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 413413Modification methylase NaeIPRO_0000087903Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP50188.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 317314SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
    Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR10629. PTHR10629. 1 hit.
    PfamiPF00145. DNA_methylase. 1 hit.
    [Graphical view]
    PRINTSiPR00105. C5METTRFRASE.
    SUPFAMiSSF53335. SSF53335. 2 hits.
    TIGRFAMsiTIGR00675. dcm. 1 hit.
    PROSITEiPS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P50188-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQSLEVVEIC AGAGGQALGL EKAGFSHRLA VELDVNAAAT LRKNLKSDVV    50
    ITGDVADPSV LNPMEHLGVS LLAGGVPCPP FSIAGKQLGA DDMRDLFAWA 100
    VELCDVMKPR ALMLENVRGL SMPRFAGYRQ HVLDRLNDMG YVAEWRLLHA 150
    SDFGVPQLRP RFVLVALQNK FAPYFTWPEP TGAAPTVGET LKDLMAADGW 200
    EGAEEWAAQA NDIAPTIVGG SKKHGGADLG PTRAKRAWAE LGVDAMGVAD 250
    APPQPGDKFK VGPKLTCEMV ARIQGWRDGE WIFEGRKTSR YRQIGNAFPP 300
    PVAEAIGKRI RAALNMEGEG RDRAVDSDHN PLYRALKESG DFMTHRQLER 350
    AVGRPIEAYE LERTISDLGR DFEVETKDGA SAMAYKLGPF KAFTGQEGHL 400
    RHEMFVRHRT KIS 413
    Length:413
    Mass (Da):45,180
    Last modified:October 1, 1996 - v1
    Checksum:i649CF1C2B97EFD28
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U09581 Genomic DNA. Translation: AAC43325.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U09581 Genomic DNA. Translation: AAC43325.1 .

    3D structure databases

    ProteinModelPortali P50188.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3452. M.NaeI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR10629. PTHR10629. 1 hit.
    Pfami PF00145. DNA_methylase. 1 hit.
    [Graphical view ]
    PRINTSi PR00105. C5METTRFRASE.
    SUPFAMi SSF53335. SSF53335. 2 hits.
    TIGRFAMsi TIGR00675. dcm. 1 hit.
    PROSITEi PS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of the NaeI restriction endonuclease-encoding gene and sequence analysis of the NaeI restriction-modification system."
      Taron C.H., van Cott E.M., Wilson G.G., Moran L.S., Slatko B.E., Hornstra L.J., Benner J.S., Kucera R.B., Guthrie E.P.
      Gene 155:19-25(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 23870 / BCRC 13661 / DSM 40034 / JCM 4614 / NBRC 13195 / NCIMB 12944 / NRRL B-3298.

    Entry informationi

    Entry nameiMTN1_NOCAE
    AccessioniPrimary (citable) accession number: P50188
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 59 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3