P50188 (MTN1_NOCAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase NaeI
Short name=M.NaeI
EC=2.1.1.37

Alternative name(s):
Cytosine-specific methyltransferase NaeI

Gene names

Name:

naeIM

Organism

Lechevalieria aerocolonigenes (Nocardia aerocolonigenes) (Saccharothrix aerocolonigenes)

Taxonomic identifier

68170 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Pseudonocardineae › Pseudonocardiaceae › Lechevalieria

Protein attributes

Sequence length	413 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GCCGGC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the NaeI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological_process	C-5 methylation of cytosine Inferred from electronic annotation. Source: GOC DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 413	413	Modification methylase NaeI		PRO_0000087903
Sites
	Active site	78	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P50188 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 649CF1C2B97EFD28	FASTA	413	45,180
	10 20 30 40 50 60 MQSLEVVEIC AGAGGQALGL EKAGFSHRLA VELDVNAAAT LRKNLKSDVV ITGDVADPSV 70 80 90 100 110 120 LNPMEHLGVS LLAGGVPCPP FSIAGKQLGA DDMRDLFAWA VELCDVMKPR ALMLENVRGL 130 140 150 160 170 180 SMPRFAGYRQ HVLDRLNDMG YVAEWRLLHA SDFGVPQLRP RFVLVALQNK FAPYFTWPEP 190 200 210 220 230 240 TGAAPTVGET LKDLMAADGW EGAEEWAAQA NDIAPTIVGG SKKHGGADLG PTRAKRAWAE 250 260 270 280 290 300 LGVDAMGVAD APPQPGDKFK VGPKLTCEMV ARIQGWRDGE WIFEGRKTSR YRQIGNAFPP 310 320 330 340 350 360 PVAEAIGKRI RAALNMEGEG RDRAVDSDHN PLYRALKESG DFMTHRQLER AVGRPIEAYE 370 380 390 400 410 LERTISDLGR DFEVETKDGA SAMAYKLGPF KAFTGQEGHL RHEMFVRHRT KIS

References

[1]

"Cloning and expression of the NaeI restriction endonuclease-encoding gene and sequence analysis of the NaeI restriction-modification system." Taron C.H., van Cott E.M., Wilson G.G., Moran L.S., Slatko B.E., Hornstra L.J., Benner J.S., Kucera R.B., Guthrie E.P. Gene 155:19-25(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 23870 / BCRC 13661 / DSM 40034 / JCM 4614 / NBRC 13195 / NCIMB 12944 / NRRL B-3298.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U09581 Genomic DNA. Translation: AAC43325.1.
3D structure databases
ProteinModelPortal	P50188.
ModBase	Search...
Protein family/group databases
REBASE	3452. M.NaeI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR018117. C5_DNA_meth_AS. IPR001525. C5_MeTfrase. [Graphical view]
PANTHER	PTHR10629. PTHR10629. 1 hit.
Pfam	PF00145. DNA_methylase. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTN1_NOCAE

Accession

Primary (citable) accession number: P50188

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program