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Protein

Modification methylase NaeI

Gene

naeIM

Organism
Lechevalieria aerocolonigenes (Nocardia aerocolonigenes) (Saccharothrix aerocolonigenes)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GCCGGC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the NaeI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei78 – 781PROSITE-ProRule annotation

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: InterPro

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3452. M.NaeI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase NaeI (EC:2.1.1.37)
Short name:
M.NaeI
Alternative name(s):
Cytosine-specific methyltransferase NaeI
Gene namesi
Name:naeIM
OrganismiLechevalieria aerocolonigenes (Nocardia aerocolonigenes) (Saccharothrix aerocolonigenes)
Taxonomic identifieri68170 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesPseudonocardineaePseudonocardiaceaeLechevalieria

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 413413Modification methylase NaeIPRO_0000087903Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP50188.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 317314SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50188-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQSLEVVEIC AGAGGQALGL EKAGFSHRLA VELDVNAAAT LRKNLKSDVV
60 70 80 90 100
ITGDVADPSV LNPMEHLGVS LLAGGVPCPP FSIAGKQLGA DDMRDLFAWA
110 120 130 140 150
VELCDVMKPR ALMLENVRGL SMPRFAGYRQ HVLDRLNDMG YVAEWRLLHA
160 170 180 190 200
SDFGVPQLRP RFVLVALQNK FAPYFTWPEP TGAAPTVGET LKDLMAADGW
210 220 230 240 250
EGAEEWAAQA NDIAPTIVGG SKKHGGADLG PTRAKRAWAE LGVDAMGVAD
260 270 280 290 300
APPQPGDKFK VGPKLTCEMV ARIQGWRDGE WIFEGRKTSR YRQIGNAFPP
310 320 330 340 350
PVAEAIGKRI RAALNMEGEG RDRAVDSDHN PLYRALKESG DFMTHRQLER
360 370 380 390 400
AVGRPIEAYE LERTISDLGR DFEVETKDGA SAMAYKLGPF KAFTGQEGHL
410
RHEMFVRHRT KIS
Length:413
Mass (Da):45,180
Last modified:October 1, 1996 - v1
Checksum:i649CF1C2B97EFD28
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09581 Genomic DNA. Translation: AAC43325.1.
RefSeqiWP_030468108.1. NZ_JOFI01000013.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09581 Genomic DNA. Translation: AAC43325.1.
RefSeqiWP_030468108.1. NZ_JOFI01000013.1.

3D structure databases

ProteinModelPortaliP50188.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi3452. M.NaeI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and expression of the NaeI restriction endonuclease-encoding gene and sequence analysis of the NaeI restriction-modification system."
    Taron C.H., van Cott E.M., Wilson G.G., Moran L.S., Slatko B.E., Hornstra L.J., Benner J.S., Kucera R.B., Guthrie E.P.
    Gene 155:19-25(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 23870 / BCRC 13661 / DSM 40034 / JCM 4614 / NBRC 13195 / NCIMB 12944 / NRRL B-3298.

Entry informationi

Entry nameiMTN1_NOCAE
AccessioniPrimary (citable) accession number: P50188
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: January 7, 2015
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.