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P50188 (MTN1_NOCAE) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase NaeI

Short name=M.NaeI
EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase NaeI
Gene names
Name:naeIM
OrganismLechevalieria aerocolonigenes (Nocardia aerocolonigenes) (Saccharothrix aerocolonigenes)
Taxonomic identifier68170 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesPseudonocardineaePseudonocardiaceaeLechevalieria

Protein attributes

Sequence length413 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence GCCGGC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the NaeI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA (cytosine-5-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 413413Modification methylase NaeI
PRO_0000087903

Regions

Domain4 – 317314SAM-dependent MTase C5-type

Sites

Active site781 By similarity

Sequences

Sequence LengthMass (Da)Tools
P50188 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 649CF1C2B97EFD28

FASTA41345,180
        10         20         30         40         50         60 
MQSLEVVEIC AGAGGQALGL EKAGFSHRLA VELDVNAAAT LRKNLKSDVV ITGDVADPSV 

        70         80         90        100        110        120 
LNPMEHLGVS LLAGGVPCPP FSIAGKQLGA DDMRDLFAWA VELCDVMKPR ALMLENVRGL 

       130        140        150        160        170        180 
SMPRFAGYRQ HVLDRLNDMG YVAEWRLLHA SDFGVPQLRP RFVLVALQNK FAPYFTWPEP 

       190        200        210        220        230        240 
TGAAPTVGET LKDLMAADGW EGAEEWAAQA NDIAPTIVGG SKKHGGADLG PTRAKRAWAE 

       250        260        270        280        290        300 
LGVDAMGVAD APPQPGDKFK VGPKLTCEMV ARIQGWRDGE WIFEGRKTSR YRQIGNAFPP 

       310        320        330        340        350        360 
PVAEAIGKRI RAALNMEGEG RDRAVDSDHN PLYRALKESG DFMTHRQLER AVGRPIEAYE 

       370        380        390        400        410 
LERTISDLGR DFEVETKDGA SAMAYKLGPF KAFTGQEGHL RHEMFVRHRT KIS 

« Hide

References

[1]"Cloning and expression of the NaeI restriction endonuclease-encoding gene and sequence analysis of the NaeI restriction-modification system."
Taron C.H., van Cott E.M., Wilson G.G., Moran L.S., Slatko B.E., Hornstra L.J., Benner J.S., Kucera R.B., Guthrie E.P.
Gene 155:19-25(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 23870 / BCRC 13661 / DSM 40034 / JCM 4614 / NBRC 13195 / NCIMB 12944 / NRRL B-3298.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U09581 Genomic DNA. Translation: AAC43325.1.

3D structure databases

ProteinModelPortalP50188.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3452. M.NaeI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.150. 1 hit.
InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
SUPFAMSSF53335. SSF53335. 2 hits.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTN1_NOCAE
AccessionPrimary (citable) accession number: P50188
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries