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Protein

Type-2 restriction enzyme NaeI

Gene

naeIR

Organism
Lechevalieria aerocolonigenes (Nocardia aerocolonigenes) (Saccharothrix aerocolonigenes)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded unmethylated sequence GCCGGC and cleaves after C-3.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi1294. NaeI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme NaeI (EC:3.1.21.4)
Short name:
R.NaeI
Alternative name(s):
Endonuclease NaeI
Type II restriction enzyme NaeI
Gene namesi
Name:naeIR
OrganismiLechevalieria aerocolonigenes (Nocardia aerocolonigenes) (Saccharothrix aerocolonigenes)
Taxonomic identifieri68170 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaPseudonocardialesPseudonocardiaceaeLechevalieria

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi43L → K: Change of function; becomes a topoisomerase that recognizes single-stranded mismatched DNA. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000773431 – 317Type-2 restriction enzyme NaeIAdd BLAST317

Interactioni

Subunit structurei

Homodimer.

Structurei

Secondary structure

1317
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi13 – 25Combined sources13
Beta strandi26 – 29Combined sources4
Helixi30 – 44Combined sources15
Helixi46 – 49Combined sources4
Helixi54 – 56Combined sources3
Helixi59 – 63Combined sources5
Helixi65 – 77Combined sources13
Beta strandi83 – 89Combined sources7
Beta strandi92 – 101Combined sources10
Helixi109 – 111Combined sources3
Beta strandi114 – 122Combined sources9
Turni123 – 126Combined sources4
Beta strandi127 – 134Combined sources8
Helixi137 – 139Combined sources3
Helixi153 – 156Combined sources4
Beta strandi160 – 163Combined sources4
Helixi173 – 175Combined sources3
Helixi179 – 186Combined sources8
Beta strandi191 – 194Combined sources4
Helixi196 – 207Combined sources12
Beta strandi210 – 213Combined sources4
Helixi215 – 222Combined sources8
Helixi228 – 230Combined sources3
Beta strandi232 – 241Combined sources10
Helixi242 – 244Combined sources3
Beta strandi246 – 249Combined sources4
Helixi255 – 261Combined sources7
Beta strandi262 – 264Combined sources3
Beta strandi272 – 279Combined sources8
Beta strandi288 – 298Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EV7X-ray2.38A/B1-317[»]
1IAWX-ray2.40A/B1-317[»]
ProteinModelPortaliP50187.
SMRiP50187.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP50187.

Family & Domainsi

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.40.600.10. 1 hit.
InterProiIPR011337. DNA_rep_MutH/RE_typeII.
IPR015210. NaeI.
IPR011335. Restrct_endonuc-II-like.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF09126. NaeI. 1 hit.
[Graphical view]
SUPFAMiSSF52980. SSF52980. 1 hit.

Sequencei

Sequence statusi: Complete.

P50187-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTELPLQFAE PDDDLERVRA TLYSLDPDGD RTAGVLRDTL DQLYDGQRTG
60 70 80 90 100
RWNFDQLHKT EKTHMGTLVE INLHREFQFG DGFETDYEIA GVQVDCKFSM
110 120 130 140 150
SQGAWMLPPE SIGHICLVIW ASDQQCAWTA GLVKVIPQFL GTANRDLKRR
160 170 180 190 200
LTPEGRAQVV KLWPDHGKLQ ENLLLHIPGD VRDQIFSAKS SRGNQHGQAR
210 220 230 240 250
VNELFRRVHG RLIGRAVIAT VAQQDDFMKR VRGSGGARSI LRPEGIIILG
260 270 280 290 300
HQDNDPKVAN DLGLPVPRKG QVVAARVVPA DEGDQRQTAE IQGRRWAVAV
310
PGDPIVEAPV VPRKSAE
Length:317
Mass (Da):35,335
Last modified:October 1, 1996 - v1
Checksum:i2D646D6814935B97
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09581 Genomic DNA. Translation: AAC43324.1.
RefSeqiWP_030468110.1. NZ_JOFI01000013.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09581 Genomic DNA. Translation: AAC43324.1.
RefSeqiWP_030468110.1. NZ_JOFI01000013.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EV7X-ray2.38A/B1-317[»]
1IAWX-ray2.40A/B1-317[»]
ProteinModelPortaliP50187.
SMRiP50187.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi1294. NaeI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP50187.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.40.600.10. 1 hit.
InterProiIPR011337. DNA_rep_MutH/RE_typeII.
IPR015210. NaeI.
IPR011335. Restrct_endonuc-II-like.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF09126. NaeI. 1 hit.
[Graphical view]
SUPFAMiSSF52980. SSF52980. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiT2N1_NOCAE
AccessioniPrimary (citable) accession number: P50187
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.