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P50185 (MTD5_DACSA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase DsaV

Short name=M.DsaV
EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase DsaV
Gene names
Name:dsaVM
OrganismDactylococcopsis salina (Myxobaktron salinum)
Taxonomic identifier292566 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesDactylococcopsis

Protein attributes

Sequence length351 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence CCNGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the DsaV endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA (cytosine-5-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 351351Modification methylase DsaV
PRO_0000087872

Regions

Domain6 – 312307SAM-dependent MTase C5-type

Sites

Active site751 By similarity

Sequences

Sequence LengthMass (Da)Tools
P50185 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 68B048AC0B8B2E5B

FASTA35140,499
        10         20         30         40         50         60 
MEKKQLKFID LFAGIGGMRI PFEELGGKCV FSSEIDKHCQ RTYEANFGEM PTGDITKLSA 

        70         80         90        100        110        120 
DSIPYHDLLL AGFPCQAFSQ GGRKQGFQDE RGQLFFQVAK ILNDHRPQAI LLENVKGLRG 

       130        140        150        160        170        180 
HDKGRTLQMI LYVLEKLNYV VSWKIISATD FNLPQKRERI FIVGFQDKNN KNLIFDFPKP 

       190        200        210        220        230        240 
IELTAKVGDL LEKEVDEKYT ITDRMWEGHQ NRKKAHRKRG NGFGFSLVNR NSSYTRTISA 

       250        260        270        280        290        300 
RYYKDGSEVL VEQANKNPRV LTPRECARLQ GFPESFVIPV SDCQAWRQFG NSVPVSVIRA 

       310        320        330        340        350 
IAQKMLSYID LTEQQKEFKK VDLDQVITQK KKQLYPEDYQ EQFIQKELAL L 

« Hide

References

[1]"DsaV methyltransferase and its isoschizomers contain a conserved segment that is similar to the segment in Hhai methyltransferase that is in contact with DNA bases."
Gopal J., Yebra M.J., Bhagwat A.S.
Nucleic Acids Res. 22:4482-4488(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: DSM 4880.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U10528 Genomic DNA. Translation: AAA86046.1.
PIRS50098.

3D structure databases

ProteinModelPortalP50185.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3361. M.DsaV.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTD5_DACSA
AccessionPrimary (citable) accession number: P50185
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries