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Protein

Modification methylase DsaV

Gene

dsaVM

Organism
Dactylococcopsis salina (Myxobaktron salinum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence CCNGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the DsaV endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei75 – 751PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3361. M.DsaV.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase DsaV (EC:2.1.1.37)
Short name:
M.DsaV
Alternative name(s):
Cytosine-specific methyltransferase DsaV
Gene namesi
Name:dsaVM
OrganismiDactylococcopsis salina (Myxobaktron salinum)
Taxonomic identifieri292566 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesDactylococcopsis

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 351351Modification methylase DsaVPRO_0000087872Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP50185.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini6 – 312307SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50185-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKKQLKFID LFAGIGGMRI PFEELGGKCV FSSEIDKHCQ RTYEANFGEM
60 70 80 90 100
PTGDITKLSA DSIPYHDLLL AGFPCQAFSQ GGRKQGFQDE RGQLFFQVAK
110 120 130 140 150
ILNDHRPQAI LLENVKGLRG HDKGRTLQMI LYVLEKLNYV VSWKIISATD
160 170 180 190 200
FNLPQKRERI FIVGFQDKNN KNLIFDFPKP IELTAKVGDL LEKEVDEKYT
210 220 230 240 250
ITDRMWEGHQ NRKKAHRKRG NGFGFSLVNR NSSYTRTISA RYYKDGSEVL
260 270 280 290 300
VEQANKNPRV LTPRECARLQ GFPESFVIPV SDCQAWRQFG NSVPVSVIRA
310 320 330 340 350
IAQKMLSYID LTEQQKEFKK VDLDQVITQK KKQLYPEDYQ EQFIQKELAL

L
Length:351
Mass (Da):40,499
Last modified:October 1, 1996 - v1
Checksum:i68B048AC0B8B2E5B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10528 Genomic DNA. Translation: AAA86046.1.
PIRiS50098.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10528 Genomic DNA. Translation: AAA86046.1.
PIRiS50098.

3D structure databases

ProteinModelPortaliP50185.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi3361. M.DsaV.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "DsaV methyltransferase and its isoschizomers contain a conserved segment that is similar to the segment in Hhai methyltransferase that is in contact with DNA bases."
    Gopal J., Yebra M.J., Bhagwat A.S.
    Nucleic Acids Res. 22:4482-4488(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DSM 4880.

Entry informationi

Entry nameiMTD5_DACSA
AccessioniPrimary (citable) accession number: P50185
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: January 7, 2015
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.