Reviewed, UniProtKB/Swiss-Prot P50185 (MTD5_DACSA)

Names and origin

Protein names

Recommended name:
    Modification methylase DsaV
      Short name=M.DsaV
    EC=2.1.1.37
Alternative name(s):
    Cytosine-specific methyltransferase DsaV

Gene names

Name:

dsaVM

Organism

Dactylococcopsis salina (Myxobaktron salinum)

Taxonomic identifier

292566 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Dactylococcopsis

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence CCNGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the DsaV endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 351	351	Modification methylase DsaV		PRO_0000087872
Sites
	Active site	75	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P50185-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 68B048AC0B8B2E5B	FASTA	351	40,499
	10 20 30 40 50 60 MEKKQLKFID LFAGIGGMRI PFEELGGKCV FSSEIDKHCQ RTYEANFGEM PTGDITKLSA 70 80 90 100 110 120 DSIPYHDLLL AGFPCQAFSQ GGRKQGFQDE RGQLFFQVAK ILNDHRPQAI LLENVKGLRG 130 140 150 160 170 180 HDKGRTLQMI LYVLEKLNYV VSWKIISATD FNLPQKRERI FIVGFQDKNN KNLIFDFPKP 190 200 210 220 230 240 IELTAKVGDL LEKEVDEKYT ITDRMWEGHQ NRKKAHRKRG NGFGFSLVNR NSSYTRTISA 250 260 270 280 290 300 RYYKDGSEVL VEQANKNPRV LTPRECARLQ GFPESFVIPV SDCQAWRQFG NSVPVSVIRA 310 320 330 340 350 IAQKMLSYID LTEQQKEFKK VDLDQVITQK KKQLYPEDYQ EQFIQKELAL L

References

[1]

"DsaV methyltransferase and its isoschizomers contain a conserved segment that is similar to the segment in Hhai methyltransferase that is in contact with DNA bases." Gopal J., Yebra M.J., Bhagwat A.S. Nucleic Acids Res. 22:4482-4488(1994) [PubMed: 7971279] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 4880.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U10528 Genomic DNA. Translation: AAA86046.1.
PIR	S50098.
3D structure databases
SMR	P50185. Positions 5-311.
ModBase	Search...
Protein family/group databases
REBASE	3361. M.DsaV.
Enzyme and pathway databases
BRENDA	2.1.1.37. 298973.
Family and domain databases
InterPro	IPR001525. C5_DNA_meth. IPR018117. C5_DNA_meth_AS. [Graphical view]
PANTHER	PTHR10629. C5_DNA_meth. 1 hit.
Pfam	PF00145. DNA_methylase. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTD5_DACSA

Accession

Primary (citable) accession number: P50185

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	February 9, 2010
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)