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P50185

- MTD5_DACSA

UniProt

P50185 - MTD5_DACSA

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Protein

Modification methylase DsaV

Gene

dsaVM

Organism
Dactylococcopsis salina (Myxobaktron salinum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence CCNGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the DsaV endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei75 – 751PROSITE-ProRule annotation

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: InterPro

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3361. M.DsaV.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase DsaV (EC:2.1.1.37)
Short name:
M.DsaV
Alternative name(s):
Cytosine-specific methyltransferase DsaV
Gene namesi
Name:dsaVM
OrganismiDactylococcopsis salina (Myxobaktron salinum)
Taxonomic identifieri292566 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesDactylococcopsis

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 351351Modification methylase DsaVPRO_0000087872Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP50185.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini6 – 312307SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10629. PTHR10629. 1 hit.
PfamiPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50185-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEKKQLKFID LFAGIGGMRI PFEELGGKCV FSSEIDKHCQ RTYEANFGEM
60 70 80 90 100
PTGDITKLSA DSIPYHDLLL AGFPCQAFSQ GGRKQGFQDE RGQLFFQVAK
110 120 130 140 150
ILNDHRPQAI LLENVKGLRG HDKGRTLQMI LYVLEKLNYV VSWKIISATD
160 170 180 190 200
FNLPQKRERI FIVGFQDKNN KNLIFDFPKP IELTAKVGDL LEKEVDEKYT
210 220 230 240 250
ITDRMWEGHQ NRKKAHRKRG NGFGFSLVNR NSSYTRTISA RYYKDGSEVL
260 270 280 290 300
VEQANKNPRV LTPRECARLQ GFPESFVIPV SDCQAWRQFG NSVPVSVIRA
310 320 330 340 350
IAQKMLSYID LTEQQKEFKK VDLDQVITQK KKQLYPEDYQ EQFIQKELAL

L
Length:351
Mass (Da):40,499
Last modified:October 1, 1996 - v1
Checksum:i68B048AC0B8B2E5B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U10528 Genomic DNA. Translation: AAA86046.1.
PIRiS50098.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U10528 Genomic DNA. Translation: AAA86046.1 .
PIRi S50098.

3D structure databases

ProteinModelPortali P50185.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3361. M.DsaV.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10629. PTHR10629. 1 hit.
Pfami PF00145. DNA_methylase. 1 hit.
[Graphical view ]
PRINTSi PR00105. C5METTRFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00675. dcm. 1 hit.
PROSITEi PS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "DsaV methyltransferase and its isoschizomers contain a conserved segment that is similar to the segment in Hhai methyltransferase that is in contact with DNA bases."
    Gopal J., Yebra M.J., Bhagwat A.S.
    Nucleic Acids Res. 22:4482-4488(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DSM 4880.

Entry informationi

Entry nameiMTD5_DACSA
AccessioniPrimary (citable) accession number: P50185
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 1, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3