Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P50183 (T2N4_NEILA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme NlaIV

Short name=R.NlaIV
EC=3.1.21.4
Alternative name(s):
Endonuclease NlaIV
Type II restriction enzyme NlaIV
Gene names
Name:nlaIVR
OrganismNeisseria lactamica
Taxonomic identifier486 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

Protein attributes

Sequence length243 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded sequence GGNNCC and cleaves after N-3.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionType II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 243243Type-2 restriction enzyme NlaIV
PRO_0000077350

Sequences

Sequence LengthMass (Da)Tools
P50183 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 3068E972D6736A49

FASTA24328,826
        10         20         30         40         50         60 
MIKLTAQQIF DKLLDEEKIL SANGQIRFFL GDVDIIVKQK DVVGNIIQEW LGGWLRKREI 

        70         80         90        100        110        120 
EFDVSTNTQM PPDFFLNKKD RSRELLEVKA FNRNASPGFD IADFKMYSDE IIHKPYMLDV 

       130        140        150        160        170        180 
DYLIFGYDMD DNGNVTIKDL WLKKVWQITR SMDGWAINLQ VKKGVVHKIR PGVWYSINKK 

       190        200        210        220        230        240 
NMPMFECLED FVSAIEETVY QNPATRHNAS LWKRKFEEAY KKHYNRSISI PRWHEIAHKY 


KKK 

« Hide

References

[1]"The NlaIV restriction and modification genes of Neisseria lactamica are flanked by leucine biosynthesis genes."
Lau P.C.K., Forghani F., Labbe D., Bergeron H., Brousseau R., Holtke H.J.
Mol. Gen. Genet. 243:24-31(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 23970 / CCUG 5853 / DSM 4691 / NCDC A7515 / NCTC 10617.
[2]Erratum
Lau P.C.K., Forghani F., Labbe D., Bergeron H., Brousseau R., Holtke H.J.
Mol. Gen. Genet. 244:167-167(1994)

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U06074 Genomic DNA. Translation: AAA53238.1.
PIRS43887.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE1342. NlaIV.

Proteomic databases

PRIDEP50183.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR019064. Restrct_endonuc_II_NlaIV.
[Graphical view]
PfamPF09564. RE_NgoBV. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT2N4_NEILA
AccessionPrimary (citable) accession number: P50183
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries