P50183 (T2N4_NEILA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-2 restriction enzyme NlaIV
Short name=R.NlaIV
EC=3.1.21.4

Alternative name(s):
Endonuclease NlaIV
Type II restriction enzyme NlaIV

Gene names

Name:

nlaIVR

Organism

Neisseria lactamica

Taxonomic identifier

486 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	243 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Recognizes the double-stranded sequence GGNNCC and cleaves after N-3.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 243	243	Type-2 restriction enzyme NlaIV		PRO_0000077350

Sequences

	Sequence		Length	Mass (Da)
	P50183 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 3068E972D6736A49	FASTA	243	28,826
	10 20 30 40 50 60 MIKLTAQQIF DKLLDEEKIL SANGQIRFFL GDVDIIVKQK DVVGNIIQEW LGGWLRKREI 70 80 90 100 110 120 EFDVSTNTQM PPDFFLNKKD RSRELLEVKA FNRNASPGFD IADFKMYSDE IIHKPYMLDV 130 140 150 160 170 180 DYLIFGYDMD DNGNVTIKDL WLKKVWQITR SMDGWAINLQ VKKGVVHKIR PGVWYSINKK 190 200 210 220 230 240 NMPMFECLED FVSAIEETVY QNPATRHNAS LWKRKFEEAY KKHYNRSISI PRWHEIAHKY KKK

References

[1]	"The NlaIV restriction and modification genes of Neisseria lactamica are flanked by leucine biosynthesis genes." Lau P.C.K., Forghani F., Labbe D., Bergeron H., Brousseau R., Holtke H.J. Mol. Gen. Genet. 243:24-31(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 23970 / CCUG 5853 / DSM 4691 / NCDC A7515 / NCTC 10617.
[2]	Erratum Lau P.C.K., Forghani F., Labbe D., Bergeron H., Brousseau R., Holtke H.J. Mol. Gen. Genet. 244:167-167(1994)

Cross-references

Sequence databases
EMBL GenBank DDBJ	U06074 Genomic DNA. Translation: AAA53238.1.
PIR	S43887.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	1342. NlaIV.
Proteomic databases
PRIDE	P50183.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR019064. Restrct_endonuc_II_NgoBV. [Graphical view]
Pfam	PF09564. RE_NgoBV. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T2N4_NEILA

Accession

Primary (citable) accession number: P50183

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program