Reviewed, UniProtKB/Swiss-Prot P50182 (MTN4_NEILA)

Names and origin

Protein names

Recommended name:
    Modification methylase NlaIV
      Short name=M.NlaIV
    EC=2.1.1.37
Alternative name(s):
    Cytosine-specific methyltransferase NlaIV

Gene names

Name:

nlaIVM

Organism

Neisseria lactamica

Taxonomic identifier

486 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GGNNCC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the NlaIV endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 423	423	Modification methylase NlaIV		PRO_0000087901
Sites
	Active site	80	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P50182-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 027E49558AEFDB19	FASTA	423	47,615
	10 20 30 40 50 60 MQQIKFIDLF SGMSGIRKGF EQACRKQSVA CKCVFTSEIK PAALEVLKQN YPDEVPYGDI 70 80 90 100 110 120 TKIETGDIPD FDILLAGFPC QAFSFAGKRL GFEDTRGTLF FDVARILKAK KPKGFILENV 130 140 150 160 170 180 EGLVTHDRKD PTQKIGRTLT VILETLEALG YYVSWKVLNA KDFGIPQNRK RIYLTGSLKS 190 200 210 220 230 240 KPDLSFETTP SPKLKNILES GLPTESSPFI KKLLKKFPPS ELYGKSVKDK RGGKNNIHSW 250 260 270 280 290 300 DIELKGAVTE EEKQLLNILL KERRKKKWAS EIGIDWMDGM PLTKAQISTF YKHPDLQNIL 310 320 330 340 350 360 DSLTDKGYLV LEHPKQKIGG QRIKDESLPK GYNIVSGKKS FEINKILDPN DVAPTLVAMD 370 380 390 400 410 420 MEHLFVVDNG GLRTLTGKEG LRLFGYPDDY SFDIPKKDKY DLLGNTVAVP VIKAVSERLL HTL

References

[1]	"The NlaIV restriction and modification genes of Neisseria lactamica are flanked by leucine biosynthesis genes." Lau P.C.K., Forghani F., Labbe D., Bergeron H., Brousseau R., Holtke H.J. Mol. Gen. Genet. 243:24-31(1994) [PubMed: 8190068] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 23970 / CCUG 5853 / DSM 4691 / NCDC A7515 / NCTC 10617.
[2]	Erratum Lau P.C.K., Forghani F., Labbe D., Bergeron H., Brousseau R., Holtke H.J. Mol. Gen. Genet. 244:167-167(1994)

Cross-references

Sequence databases
EMBL GenBank DDBJ	U06074 Genomic DNA. Translation: AAA53237.1.
PIR	S43886.
3D structure databases
SMR	P50182. Positions 1-423.
ModBase	Search...
Protein family/group databases
REBASE	3469. M.NlaIV.
Enzyme and pathway databases
BRENDA	2.1.1.37. 96206.
Family and domain databases
InterPro	IPR001525. C5_DNA_meth. IPR018117. C5_DNA_meth_AS. [Graphical view]
PANTHER	PTHR10629. C5_DNA_meth. 1 hit.
Pfam	PF00145. DNA_methylase. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTN4_NEILA

Accession

Primary (citable) accession number: P50182

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	February 9, 2010
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)