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Protein

Modification methylase NlaIV

Gene

nlaIVM

Organism
Neisseria lactamica
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GGNNCC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the NlaIV endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei80PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processRestriction system
LigandS-adenosyl-L-methionine

Protein family/group databases

REBASEi3469. M.NlaIV.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase NlaIV (EC:2.1.1.37)
Short name:
M.NlaIV
Alternative name(s):
Cytosine-specific methyltransferase NlaIV
Gene namesi
Name:nlaIVM
OrganismiNeisseria lactamica
Taxonomic identifieri486 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000879011 – 423Modification methylase NlaIVAdd BLAST423

Interactioni

Protein-protein interaction databases

STRINGi489653.NLA_10840.

Structurei

3D structure databases

ProteinModelPortaliP50182.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 423SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd BLAST420

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4107V6I. Bacteria.
COG0270. LUCA.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiView protein in InterPro
IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
PfamiView protein in Pfam
PF00145. DNA_methylase. 1 hit.
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiView protein in PROSITE
PS00094. C5_MTASE_1. 1 hit.
PS51679. SAM_MT_C5. 1 hit.

Sequencei

Sequence statusi: Complete.

P50182-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQQIKFIDLF SGMSGIRKGF EQACRKQSVA CKCVFTSEIK PAALEVLKQN
60 70 80 90 100
YPDEVPYGDI TKIETGDIPD FDILLAGFPC QAFSFAGKRL GFEDTRGTLF
110 120 130 140 150
FDVARILKAK KPKGFILENV EGLVTHDRKD PTQKIGRTLT VILETLEALG
160 170 180 190 200
YYVSWKVLNA KDFGIPQNRK RIYLTGSLKS KPDLSFETTP SPKLKNILES
210 220 230 240 250
GLPTESSPFI KKLLKKFPPS ELYGKSVKDK RGGKNNIHSW DIELKGAVTE
260 270 280 290 300
EEKQLLNILL KERRKKKWAS EIGIDWMDGM PLTKAQISTF YKHPDLQNIL
310 320 330 340 350
DSLTDKGYLV LEHPKQKIGG QRIKDESLPK GYNIVSGKKS FEINKILDPN
360 370 380 390 400
DVAPTLVAMD MEHLFVVDNG GLRTLTGKEG LRLFGYPDDY SFDIPKKDKY
410 420
DLLGNTVAVP VIKAVSERLL HTL
Length:423
Mass (Da):47,615
Last modified:October 1, 1996 - v1
Checksum:i027E49558AEFDB19
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U06074 Genomic DNA. Translation: AAA53237.1.
PIRiS43886.

Genome annotation databases

GeneIDi29671956.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U06074 Genomic DNA. Translation: AAA53237.1.
PIRiS43886.

3D structure databases

ProteinModelPortaliP50182.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi489653.NLA_10840.

Protein family/group databases

REBASEi3469. M.NlaIV.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi29671956.

Phylogenomic databases

eggNOGiENOG4107V6I. Bacteria.
COG0270. LUCA.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiView protein in InterPro
IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
PfamiView protein in Pfam
PF00145. DNA_methylase. 1 hit.
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiView protein in PROSITE
PS00094. C5_MTASE_1. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMTN4_NEILA
AccessioniPrimary (citable) accession number: P50182
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: February 15, 2017
This is version 65 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.