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P50182 (MTN4_NEILA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase NlaIV

Short name=M.NlaIV
EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase NlaIV
Gene names
Name:nlaIVM
OrganismNeisseria lactamica
Taxonomic identifier486 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

Protein attributes

Sequence length423 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence GGNNCC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the NlaIV endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA (cytosine-5-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 423423Modification methylase NlaIV
PRO_0000087901

Regions

Domain4 – 423420SAM-dependent MTase C5-type

Sites

Active site801 By similarity

Sequences

Sequence LengthMass (Da)Tools
P50182 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 027E49558AEFDB19

FASTA42347,615
        10         20         30         40         50         60 
MQQIKFIDLF SGMSGIRKGF EQACRKQSVA CKCVFTSEIK PAALEVLKQN YPDEVPYGDI 

        70         80         90        100        110        120 
TKIETGDIPD FDILLAGFPC QAFSFAGKRL GFEDTRGTLF FDVARILKAK KPKGFILENV 

       130        140        150        160        170        180 
EGLVTHDRKD PTQKIGRTLT VILETLEALG YYVSWKVLNA KDFGIPQNRK RIYLTGSLKS 

       190        200        210        220        230        240 
KPDLSFETTP SPKLKNILES GLPTESSPFI KKLLKKFPPS ELYGKSVKDK RGGKNNIHSW 

       250        260        270        280        290        300 
DIELKGAVTE EEKQLLNILL KERRKKKWAS EIGIDWMDGM PLTKAQISTF YKHPDLQNIL 

       310        320        330        340        350        360 
DSLTDKGYLV LEHPKQKIGG QRIKDESLPK GYNIVSGKKS FEINKILDPN DVAPTLVAMD 

       370        380        390        400        410        420 
MEHLFVVDNG GLRTLTGKEG LRLFGYPDDY SFDIPKKDKY DLLGNTVAVP VIKAVSERLL 


HTL 

« Hide

References

[1]"The NlaIV restriction and modification genes of Neisseria lactamica are flanked by leucine biosynthesis genes."
Lau P.C.K., Forghani F., Labbe D., Bergeron H., Brousseau R., Holtke H.J.
Mol. Gen. Genet. 243:24-31(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 23970 / CCUG 5853 / DSM 4691 / NCDC A7515 / NCTC 10617.
[2]Erratum
Lau P.C.K., Forghani F., Labbe D., Bergeron H., Brousseau R., Holtke H.J.
Mol. Gen. Genet. 244:167-167(1994)

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U06074 Genomic DNA. Translation: AAA53237.1.
PIRS43886.

3D structure databases

ProteinModelPortalP50182.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3469. M.NlaIV.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTN4_NEILA
AccessionPrimary (citable) accession number: P50182
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries