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P50179 (MTL21_LACLC) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase LlaDCHIA

Short name=M.LlaDCHI A
Short name=M.LlaDCHIA
EC=2.1.1.72
Alternative name(s):
Adenine-specific methyltransferase LlaDCHIA
M.LlaII A
Gene names
Name:llaDCHIA
Synonyms:llaDCHIAM, llaIIA
Encoded onPlasmid pSRQ700
OrganismLactococcus lactis subsp. cremoris (Streptococcus cremoris)
Taxonomic identifier1359 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

Protein attributes

Sequence length284 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the LlaDCHI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Miscellaneous

The LlaDCHI restriction system has two different methylases.

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termPlasmid
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionnucleic acid binding

Inferred from electronic annotation. Source: InterPro

site-specific DNA-methyltransferase (adenine-specific) activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 284284Modification methylase LlaDCHIA
PRO_0000087953

Sites

Binding site171S-adenosyl-L-methionine By similarity
Binding site211S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding site621S-adenosyl-L-methionine By similarity
Binding site1941S-adenosyl-L-methionine By similarity

Sequences

Sequence LengthMass (Da)Tools
P50179 [UniParc].

Last modified May 15, 2002. Version 2.
Checksum: C46064217FC4FB09

FASTA28433,078
        10         20         30         40         50         60 
MNLLQKNKIN LRPFTKWTGG KRQLLPHIQY LMPEKYNHFF EPFIGGGALF FELAPQKAVI 

        70         80         90        100        110        120 
NDFNSELINC YRQMKDNPEQ LIELLTNHQR ENSKEYYLDL RSSDRDGRID KMSEVERAAR 

       130        140        150        160        170        180 
IMYMLRVDFN GLYRVNSKNQ FNVPYGRYKN PKIVDKELIE SISEYLNNNS IKIMSGDFEK 

       190        200        210        220        230        240 
AVKEAQDGDF VYFDPPYIPL SETSAFTSYT HEGFSYEDQV RLRDCFKQLD SKGVFVMLSN 

       250        260        270        280 
SSSPLAEELY KDFNIHKIEA TRTNGAKSSS RGKITEIIVT NYGN 

« Hide

References

[1]"Cloning and sequencing of LlaDCHI restriction/modification genes from Lactococcus lactis and relatedness of this system to the Streptococcus pneumoniae DpnII system."
Moineau S., Walker S.A., Vedamuthu E.R., Vandenbergh P.A.
Appl. Environ. Microbiol. 61:2193-2202(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: DCH-4.
[2]Erratum
Moineau S., Walker S.A., Vedamuthu E.R., Vandenbergh P.A.
Appl. Environ. Microbiol. 61:3514-3514(1995)
[3]"DNA sequence analysis of three Lactococcus lactis plasmids encoding phage resistance mechanisms."
Boucher I., Emond E., Parrot M., Moineau S.
J. Dairy Sci. 84:1610-1620(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U16027 Genomic DNA. Translation: AAK57808.1.
RefSeqNP_116733.1. NC_002798.1.

3D structure databases

ProteinModelPortalP50179.
SMRP50179. Positions 11-282.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3662. M1.LlaDCHI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1113498.

Phylogenomic databases

ProtClustDBCLSK689434.

Family and domain databases

Gene3D1.10.1020.10. 1 hit.
InterProIPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
[Graphical view]
PfamPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PIRSFPIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSPR00505. D12N6MTFRASE.
TIGRFAMsTIGR00571. dam. 1 hit.
PROSITEPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTL21_LACLC
AccessionPrimary (citable) accession number: P50179
Secondary accession number(s): Q93K25
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: May 15, 2002
Last modified: October 16, 2013
This is version 69 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries