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P50179

- MTL21_LACLC

UniProt

P50179 - MTL21_LACLC

Protein

Modification methylase LlaDCHIA

Gene

llaDCHIA

Organism
Lactococcus lactis subsp. cremoris (Streptococcus cremoris)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 2 (15 May 2002)
      Previous versions | rss
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    Functioni

    This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the LlaDCHI endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei17 – 171S-adenosyl-L-methionineBy similarity
    Binding sitei21 – 211S-adenosyl-L-methionine; via amide nitrogenBy similarity
    Binding sitei62 – 621S-adenosyl-L-methionineBy similarity
    Binding sitei194 – 1941S-adenosyl-L-methionineBy similarity

    GO - Molecular functioni

    1. nucleic acid binding Source: InterPro
    2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3662. M1.LlaDCHI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase LlaDCHIA (EC:2.1.1.72)
    Short name:
    M.LlaDCHI A
    Short name:
    M.LlaDCHIA
    Alternative name(s):
    Adenine-specific methyltransferase LlaDCHIA
    M.LlaII A
    Gene namesi
    Name:llaDCHIA
    Synonyms:llaDCHIAM, llaIIA
    Encoded oniPlasmid pSRQ7000 Publication
    OrganismiLactococcus lactis subsp. cremoris (Streptococcus cremoris)
    Taxonomic identifieri1359 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 284284Modification methylase LlaDCHIAPRO_0000087953Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP50179.
    SMRiP50179. Positions 11-282.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the N(4)/N(6)-methyltransferase family.Curated

    Family and domain databases

    Gene3Di1.10.1020.10. 1 hit.
    3.40.50.150. 2 hits.
    InterProiIPR023095. Ade_MeTrfase_dom_2.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR012263. M_m6A_EcoRV.
    IPR012327. MeTrfase_D12.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF02086. MethyltransfD12. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000398. M_m6A_EcoRV. 1 hit.
    PRINTSiPR00505. D12N6MTFRASE.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00571. dam. 1 hit.
    PROSITEiPS00092. N6_MTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P50179-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNLLQKNKIN LRPFTKWTGG KRQLLPHIQY LMPEKYNHFF EPFIGGGALF    50
    FELAPQKAVI NDFNSELINC YRQMKDNPEQ LIELLTNHQR ENSKEYYLDL 100
    RSSDRDGRID KMSEVERAAR IMYMLRVDFN GLYRVNSKNQ FNVPYGRYKN 150
    PKIVDKELIE SISEYLNNNS IKIMSGDFEK AVKEAQDGDF VYFDPPYIPL 200
    SETSAFTSYT HEGFSYEDQV RLRDCFKQLD SKGVFVMLSN SSSPLAEELY 250
    KDFNIHKIEA TRTNGAKSSS RGKITEIIVT NYGN 284
    Length:284
    Mass (Da):33,078
    Last modified:May 15, 2002 - v2
    Checksum:iC46064217FC4FB09
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U16027 Genomic DNA. Translation: AAK57808.1.
    RefSeqiNP_116733.1. NC_002798.1.

    Genome annotation databases

    GeneIDi1113498.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U16027 Genomic DNA. Translation: AAK57808.1 .
    RefSeqi NP_116733.1. NC_002798.1.

    3D structure databases

    ProteinModelPortali P50179.
    SMRi P50179. Positions 11-282.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3662. M1.LlaDCHI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1113498.

    Family and domain databases

    Gene3Di 1.10.1020.10. 1 hit.
    3.40.50.150. 2 hits.
    InterProi IPR023095. Ade_MeTrfase_dom_2.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR012263. M_m6A_EcoRV.
    IPR012327. MeTrfase_D12.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF02086. MethyltransfD12. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000398. M_m6A_EcoRV. 1 hit.
    PRINTSi PR00505. D12N6MTFRASE.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00571. dam. 1 hit.
    PROSITEi PS00092. N6_MTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing of LlaDCHI restriction/modification genes from Lactococcus lactis and relatedness of this system to the Streptococcus pneumoniae DpnII system."
      Moineau S., Walker S.A., Vedamuthu E.R., Vandenbergh P.A.
      Appl. Environ. Microbiol. 61:2193-2202(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: DCH-4.
    2. Erratum
      Moineau S., Walker S.A., Vedamuthu E.R., Vandenbergh P.A.
      Appl. Environ. Microbiol. 61:3514-3514(1995)
    3. "DNA sequence analysis of three Lactococcus lactis plasmids encoding phage resistance mechanisms."
      Boucher I., Emond E., Parrot M., Moineau S.
      J. Dairy Sci. 84:1610-1620(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: SEQUENCE REVISION.

    Entry informationi

    Entry nameiMTL21_LACLC
    AccessioniPrimary (citable) accession number: P50179
    Secondary accession number(s): Q93K25
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: May 15, 2002
    Last modified: October 1, 2014
    This is version 73 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The LlaDCHI restriction system has two different methylases.

    Keywords - Technical termi

    Plasmid

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3