P50179 (MTL21_LACLC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase LlaDCHIA
Short name=M.LlaDCHI A
Short name=M.LlaDCHIA
EC=2.1.1.72

Alternative name(s):
Adenine-specific methyltransferase LlaDCHIA
M.LlaII A

Gene names

Name:	llaDCHIA
Synonyms:	llaDCHIAM, llaIIA

Encoded on

Plasmid pSRQ700

Organism

Lactococcus lactis subsp. cremoris (Streptococcus cremoris)

Taxonomic identifier

1359 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the LlaDCHI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Miscellaneous	The LlaDCHI restriction system has two different methylases.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	Modification methylase LlaDCHIA		PRO_0000087953
Sites
	Binding site	17	1	S-adenosyl-L-methionine By similarity
	Binding site	21	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	62	1	S-adenosyl-L-methionine By similarity
	Binding site	194	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P50179 [UniParc]. Last modified May 15, 2002. Version 2. Checksum: C46064217FC4FB09	FASTA	284	33,078
	10 20 30 40 50 60 MNLLQKNKIN LRPFTKWTGG KRQLLPHIQY LMPEKYNHFF EPFIGGGALF FELAPQKAVI 70 80 90 100 110 120 NDFNSELINC YRQMKDNPEQ LIELLTNHQR ENSKEYYLDL RSSDRDGRID KMSEVERAAR 130 140 150 160 170 180 IMYMLRVDFN GLYRVNSKNQ FNVPYGRYKN PKIVDKELIE SISEYLNNNS IKIMSGDFEK 190 200 210 220 230 240 AVKEAQDGDF VYFDPPYIPL SETSAFTSYT HEGFSYEDQV RLRDCFKQLD SKGVFVMLSN 250 260 270 280 SSSPLAEELY KDFNIHKIEA TRTNGAKSSS RGKITEIIVT NYGN

References

[1]	"Cloning and sequencing of LlaDCHI restriction/modification genes from Lactococcus lactis and relatedness of this system to the Streptococcus pneumoniae DpnII system." Moineau S., Walker S.A., Vedamuthu E.R., Vandenbergh P.A. Appl. Environ. Microbiol. 61:2193-2202(1995) [PubMed: 7793939] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DCH-4.
[2]	Erratum Moineau S., Walker S.A., Vedamuthu E.R., Vandenbergh P.A. Appl. Environ. Microbiol. 61:3514-3514(1995)
[3]	"DNA sequence analysis of three Lactococcus lactis plasmids encoding phage resistance mechanisms." Boucher I., Emond E., Parrot M., Moineau S. J. Dairy Sci. 84:1610-1620(2001) [PubMed: 11467810] [Abstract] Cited for: SEQUENCE REVISION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U16027 Genomic DNA. Translation: AAK57808.1.
RefSeq	NP_116733.1. NC_002798.1.
3D structure databases
ProteinModelPortal	P50179.
SMR	P50179. Positions 11-282.
ModBase	Search...
Protein family/group databases
REBASE	3662. M1.LlaDCHI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1113498.
Phylogenomic databases
ProtClustDB	CLSK689434.
Family and domain databases
InterPro	IPR023095. Ade_MeTrfase_dom_2. IPR002052. DNA_methylase_N6_adenine_CS. IPR012263. M_m6A_EcoRV. IPR012327. MeTrfase_D12. [Graphical view]
Gene3D	G3DSA:1.10.1020.10. Ade_MeTrfase_dom_2. 1 hit.
Pfam	PF02086. MethyltransfD12. 1 hit. [Graphical view]
PIRSF	PIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTS	PR00505. D12N6MTFRASE.
TIGRFAMs	TIGR00571. Dam. 1 hit.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTL21_LACLC

Accession

Primary (citable) accession number: P50179
Secondary accession number(s): Q93K25

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	May 15, 2002
Last modified:	May 31, 2011
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program