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Protein

Modification methylase LlaDCHIB

Gene

llaDCHIBM

Organism
Lactococcus lactis subsp. cremoris (Streptococcus cremoris)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the LlaDCHI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. N-methyltransferase activity Source: InterPro
  3. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3663. M2.LlaDCHI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase LlaDCHIB (EC:2.1.1.72)
Short name:
M.LlaDCHI B
Short name:
M.LlaDCHIB
Alternative name(s):
Adenine-specific methyltransferase LlaDCHIB
M.LlaII B
Gene namesi
Name:llaDCHIBM
Synonyms:llaIIB
Encoded oniPlasmid pSRQ7000 Publication
OrganismiLactococcus lactis subsp. cremoris (Streptococcus cremoris)
Taxonomic identifieri1359 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 269269Modification methylase LlaDCHIBPRO_0000087954Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP50178.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR001091. RM_Methylase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PRINTSiPR00508. S21N4MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50178-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAINEYKYGG VLMTKPYYEK ENAILVHADS FKLLEKIKPE SMDMIFADPP
60 70 80 90 100
YFLSNGGMSN SGGQIVSVDK GDWDKISSFE EKHDFNRRWI RLARLVLKPN
110 120 130 140 150
GTIWVSGSLH NIYSVGMALE QEGFKILNNI TWQKTNPAPN LSCRYFTHST
160 170 180 190 200
ETILWARKND KKSRHYYNYE LMKEFNDGKQ MKDVWTGSLT KKSEKWAGKH
210 220 230 240 250
PTQKPEYILE RIILASTKEN DYILDPFVGS GTTGVVAKRL GRKFIGIDSE
260
KEYLKIAKKR LNKGATYGL
Length:269
Mass (Da):30,925
Last modified:October 1, 1996 - v1
Checksum:i0AB61895696B46EA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U16027 Genomic DNA. Translation: AAB06312.1.
RefSeqiNP_116732.1. NC_002798.1.

Genome annotation databases

GeneIDi1113499.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U16027 Genomic DNA. Translation: AAB06312.1.
RefSeqiNP_116732.1. NC_002798.1.

3D structure databases

ProteinModelPortaliP50178.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi3663. M2.LlaDCHI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1113499.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR001091. RM_Methylase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PRINTSiPR00508. S21N4MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and sequencing of LlaDCHI restriction/modification genes from Lactococcus lactis and relatedness of this system to the Streptococcus pneumoniae DpnII system."
    Moineau S., Walker S.A., Vedamuthu E.R., Vandenbergh P.A.
    Appl. Environ. Microbiol. 61:2193-2202(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DCH-4.

Entry informationi

Entry nameiMTL22_LACLC
AccessioniPrimary (citable) accession number: P50178
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: January 7, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The LlaII restriction system has two different methylases.

Keywords - Technical termi

Plasmid

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.