Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P50178 (MTL22_LACLC) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase LlaDCHIB

Short name=M.LlaDCHI B
Short name=M.LlaDCHIB
EC=2.1.1.72
Alternative name(s):
Adenine-specific methyltransferase LlaDCHIB
M.LlaII B
Gene names
Name:llaDCHIBM
Synonyms:llaIIB
Encoded onPlasmid pSRQ700
OrganismLactococcus lactis subsp. cremoris (Streptococcus cremoris)
Taxonomic identifier1359 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

Protein attributes

Sequence length269 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the LlaDCHI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Miscellaneous

The LlaII restriction system has two different methylases.

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 269269Modification methylase LlaDCHIB
PRO_0000087954

Sequences

Sequence LengthMass (Da)Tools
P50178 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 0AB61895696B46EA

FASTA26930,925
        10         20         30         40         50         60 
MAINEYKYGG VLMTKPYYEK ENAILVHADS FKLLEKIKPE SMDMIFADPP YFLSNGGMSN 

        70         80         90        100        110        120 
SGGQIVSVDK GDWDKISSFE EKHDFNRRWI RLARLVLKPN GTIWVSGSLH NIYSVGMALE 

       130        140        150        160        170        180 
QEGFKILNNI TWQKTNPAPN LSCRYFTHST ETILWARKND KKSRHYYNYE LMKEFNDGKQ 

       190        200        210        220        230        240 
MKDVWTGSLT KKSEKWAGKH PTQKPEYILE RIILASTKEN DYILDPFVGS GTTGVVAKRL 

       250        260 
GRKFIGIDSE KEYLKIAKKR LNKGATYGL 

« Hide

References

[1]"Cloning and sequencing of LlaDCHI restriction/modification genes from Lactococcus lactis and relatedness of this system to the Streptococcus pneumoniae DpnII system."
Moineau S., Walker S.A., Vedamuthu E.R., Vandenbergh P.A.
Appl. Environ. Microbiol. 61:2193-2202(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: DCH-4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U16027 Genomic DNA. Translation: AAB06312.1.
RefSeqNP_116732.1. NC_002798.1.

3D structure databases

ProteinModelPortalP50178.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3663. M2.LlaDCHI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1113499.

Phylogenomic databases

ProtClustDBCLSK785445.

Family and domain databases

InterProIPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR001091. RM_Methylase.
[Graphical view]
PfamPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PRINTSPR00508. S21N4MTFRASE.
PROSITEPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTL22_LACLC
AccessionPrimary (citable) accession number: P50178
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries