Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P50178

- MTL22_LACLC

UniProt

P50178 - MTL22_LACLC

Protein

Modification methylase LlaDCHIB

Gene

llaDCHIBM

Organism
Lactococcus lactis subsp. cremoris (Streptococcus cremoris)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the LlaDCHI endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. N-methyltransferase activity Source: InterPro
    3. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3663. M2.LlaDCHI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase LlaDCHIB (EC:2.1.1.72)
    Short name:
    M.LlaDCHI B
    Short name:
    M.LlaDCHIB
    Alternative name(s):
    Adenine-specific methyltransferase LlaDCHIB
    M.LlaII B
    Gene namesi
    Name:llaDCHIBM
    Synonyms:llaIIB
    Encoded oniPlasmid pSRQ7000 Publication
    OrganismiLactococcus lactis subsp. cremoris (Streptococcus cremoris)
    Taxonomic identifieri1359 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 269269Modification methylase LlaDCHIBPRO_0000087954Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP50178.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the N(4)/N(6)-methyltransferase family.Curated

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR002941. DNA_methylase_N4/N6.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR001091. RM_Methylase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF01555. N6_N4_Mtase. 1 hit.
    [Graphical view]
    PRINTSiPR00508. S21N4MTFRASE.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS00092. N6_MTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P50178-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAINEYKYGG VLMTKPYYEK ENAILVHADS FKLLEKIKPE SMDMIFADPP    50
    YFLSNGGMSN SGGQIVSVDK GDWDKISSFE EKHDFNRRWI RLARLVLKPN 100
    GTIWVSGSLH NIYSVGMALE QEGFKILNNI TWQKTNPAPN LSCRYFTHST 150
    ETILWARKND KKSRHYYNYE LMKEFNDGKQ MKDVWTGSLT KKSEKWAGKH 200
    PTQKPEYILE RIILASTKEN DYILDPFVGS GTTGVVAKRL GRKFIGIDSE 250
    KEYLKIAKKR LNKGATYGL 269
    Length:269
    Mass (Da):30,925
    Last modified:October 1, 1996 - v1
    Checksum:i0AB61895696B46EA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U16027 Genomic DNA. Translation: AAB06312.1.
    RefSeqiNP_116732.1. NC_002798.1.

    Genome annotation databases

    GeneIDi1113499.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U16027 Genomic DNA. Translation: AAB06312.1 .
    RefSeqi NP_116732.1. NC_002798.1.

    3D structure databases

    ProteinModelPortali P50178.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3663. M2.LlaDCHI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1113499.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR002941. DNA_methylase_N4/N6.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR001091. RM_Methylase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF01555. N6_N4_Mtase. 1 hit.
    [Graphical view ]
    PRINTSi PR00508. S21N4MTFRASE.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS00092. N6_MTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing of LlaDCHI restriction/modification genes from Lactococcus lactis and relatedness of this system to the Streptococcus pneumoniae DpnII system."
      Moineau S., Walker S.A., Vedamuthu E.R., Vandenbergh P.A.
      Appl. Environ. Microbiol. 61:2193-2202(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: DCH-4.

    Entry informationi

    Entry nameiMTL22_LACLC
    AccessioniPrimary (citable) accession number: P50178
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The LlaII restriction system has two different methylases.

    Keywords - Technical termi

    Plasmid

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3