P50178 (MTL22_LACLC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase LlaDCHIB
Short name=M.LlaDCHI B
Short name=M.LlaDCHIB
EC=2.1.1.72

Alternative name(s):
Adenine-specific methyltransferase LlaDCHIB
M.LlaII B

Gene names

Name:	llaDCHIBM
Synonyms:	llaIIB

Encoded on

Plasmid pSRQ700

Organism

Lactococcus lactis subsp. cremoris (Streptococcus cremoris)

Taxonomic identifier

1359 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Lactococcus › Lactococcus lactis

Protein attributes

Sequence length	269 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the LlaDCHI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Miscellaneous	The LlaII restriction system has two different methylases.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Plasmid
Gene Ontology (GO)
Biological_process	DNA methylation on adenine Inferred from electronic annotation. Source: GOC DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 269	269	Modification methylase LlaDCHIB		PRO_0000087954

Sequences

	Sequence		Length	Mass (Da)
	P50178 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 0AB61895696B46EA	FASTA	269	30,925
	10 20 30 40 50 60 MAINEYKYGG VLMTKPYYEK ENAILVHADS FKLLEKIKPE SMDMIFADPP YFLSNGGMSN 70 80 90 100 110 120 SGGQIVSVDK GDWDKISSFE EKHDFNRRWI RLARLVLKPN GTIWVSGSLH NIYSVGMALE 130 140 150 160 170 180 QEGFKILNNI TWQKTNPAPN LSCRYFTHST ETILWARKND KKSRHYYNYE LMKEFNDGKQ 190 200 210 220 230 240 MKDVWTGSLT KKSEKWAGKH PTQKPEYILE RIILASTKEN DYILDPFVGS GTTGVVAKRL 250 260 GRKFIGIDSE KEYLKIAKKR LNKGATYGL

References

[1]

"Cloning and sequencing of LlaDCHI restriction/modification genes from Lactococcus lactis and relatedness of this system to the Streptococcus pneumoniae DpnII system." Moineau S., Walker S.A., Vedamuthu E.R., Vandenbergh P.A. Appl. Environ. Microbiol. 61:2193-2202(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DCH-4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U16027 Genomic DNA. Translation: AAB06312.1.
RefSeq	NP_116732.1. NC_002798.1.
3D structure databases
ProteinModelPortal	P50178.
ModBase	Search...
Protein family/group databases
REBASE	3663. M2.LlaDCHI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1113499.
Phylogenomic databases
ProtClustDB	CLSK785445.
Family and domain databases
InterPro	IPR002941. DNA_methylase_N4/N6. IPR002052. DNA_methylase_N6_adenine_CS. IPR001091. RM_Methylase. [Graphical view]
Pfam	PF01555. N6_N4_Mtase. 1 hit. [Graphical view]
PRINTS	PR00508. S21N4MTFRASE.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTL22_LACLC

Accession

Primary (citable) accession number: P50178

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program