Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P50177

- T2L2_LACLC

UniProt

P50177 - T2L2_LACLC

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Type-2 restriction enzyme LlaDCHI

Gene

llaDCHIR

Organism
Lactococcus lactis subsp. cremoris (Streptococcus cremoris)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Recognizes the double-stranded unmethylated sequence GATC and cleaves before G-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi2772. LlaDCHI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme LlaDCHI (EC:3.1.21.4)
Short name:
R.LlaDCHI
Alternative name(s):
Endonuclease LlaDCHI
LlaII
Type II restriction enzyme LlaDCHI
Gene namesi
Name:llaDCHIR
Synonyms:llaIIC
Encoded oniPlasmid pSRQ7000 Publication
OrganismiLactococcus lactis subsp. cremoris (Streptococcus cremoris)
Taxonomic identifieri1359 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 304304Type-2 restriction enzyme LlaDCHIPRO_0000077328Add
BLAST

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR021191. Restrct_endonuc_II_DpmII.
IPR007637. Restrct_endonuc_II_DpmII-like.
[Graphical view]
PfamiPF04556. DpnII. 1 hit.
[Graphical view]
PIRSFiPIRSF016080. Restrict_endonuc_II_DpmII. 1 hit.

Sequencei

Sequence statusi: Complete.

P50177 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDFNNYIGLE SDDRLNAFMA TLSVTNRTPE YYVNWEKVER ETRKFELELN
60 70 80 90 100
TLNYLIGKED IYSEALELFT NQPELLKAIP SLIASRDTSL DILNIDENDD
110 120 130 140 150
MSFEQLNFLV IDENCIADYV DFINQAGLLD FLQNKAKRSL VDYVYGVEAG
160 170 180 190 200
LDSNARKTRS GTTMEGILER TVSKIAQGKG LDWKPQATAS FIKSQWDIEV
210 220 230 240 250
PVDKSKRRFD AAVYSRALNK VWLIETNYYG GGGSKLKAVA GEFTELSQFV
260 270 280 290 300
KTSKDNVEFV WVTDGQGWKF SRLPLAEAFG HIDNVFNLTM LKEGFLSDLF

EKEI
Length:304
Mass (Da):34,670
Last modified:October 1, 1996 - v1
Checksum:iC0C84547A28A20E4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U16027 Genomic DNA. Translation: AAB06313.1.
RefSeqiNP_116731.1. NC_002798.1.

Genome annotation databases

GeneIDi1113500.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U16027 Genomic DNA. Translation: AAB06313.1 .
RefSeqi NP_116731.1. NC_002798.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 2772. LlaDCHI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1113500.

Family and domain databases

InterProi IPR021191. Restrct_endonuc_II_DpmII.
IPR007637. Restrct_endonuc_II_DpmII-like.
[Graphical view ]
Pfami PF04556. DpnII. 1 hit.
[Graphical view ]
PIRSFi PIRSF016080. Restrict_endonuc_II_DpmII. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning and sequencing of LlaDCHI restriction/modification genes from Lactococcus lactis and relatedness of this system to the Streptococcus pneumoniae DpnII system."
    Moineau S., Walker S.A., Vedamuthu E.R., Vandenbergh P.A.
    Appl. Environ. Microbiol. 61:2193-2202(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DCH-4.

Entry informationi

Entry nameiT2L2_LACLC
AccessioniPrimary (citable) accession number: P50177
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 1, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3