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P50177 (T2L2_LACLC) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme LlaDCHI

Short name=R.LlaDCHI
EC=3.1.21.4
Alternative name(s):
Endonuclease LlaDCHI
LlaII
Type II restriction enzyme LlaDCHI
Gene names
Name:llaDCHIR
Synonyms:llaIIC
Encoded onPlasmid pSRQ700
OrganismLactococcus lactis subsp. cremoris (Streptococcus cremoris)
Taxonomic identifier1359 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

Protein attributes

Sequence length304 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Recognizes the double-stranded unmethylated sequence GATC and cleaves before G-1.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Sequence similarities

Belongs to the type II restriction enzyme DpnII family.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical termPlasmid
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

Type II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 304304Type-2 restriction enzyme LlaDCHI
PRO_0000077328

Sequences

Sequence LengthMass (Da)Tools
P50177 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: C0C84547A28A20E4

FASTA30434,670
        10         20         30         40         50         60 
MDFNNYIGLE SDDRLNAFMA TLSVTNRTPE YYVNWEKVER ETRKFELELN TLNYLIGKED 

        70         80         90        100        110        120 
IYSEALELFT NQPELLKAIP SLIASRDTSL DILNIDENDD MSFEQLNFLV IDENCIADYV 

       130        140        150        160        170        180 
DFINQAGLLD FLQNKAKRSL VDYVYGVEAG LDSNARKTRS GTTMEGILER TVSKIAQGKG 

       190        200        210        220        230        240 
LDWKPQATAS FIKSQWDIEV PVDKSKRRFD AAVYSRALNK VWLIETNYYG GGGSKLKAVA 

       250        260        270        280        290        300 
GEFTELSQFV KTSKDNVEFV WVTDGQGWKF SRLPLAEAFG HIDNVFNLTM LKEGFLSDLF 


EKEI 

« Hide

References

[1]"Cloning and sequencing of LlaDCHI restriction/modification genes from Lactococcus lactis and relatedness of this system to the Streptococcus pneumoniae DpnII system."
Moineau S., Walker S.A., Vedamuthu E.R., Vandenbergh P.A.
Appl. Environ. Microbiol. 61:2193-2202(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: DCH-4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U16027 Genomic DNA. Translation: AAB06313.1.
RefSeqNP_116731.1. NC_002798.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE2772. LlaDCHI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1113500.

Phylogenomic databases

ProtClustDBCLSK2747636.

Family and domain databases

InterProIPR021191. Restrct_endonuc_II_DpmII.
IPR007637. Restrct_endonuc_II_DpmII-like.
[Graphical view]
PfamPF04556. DpnII. 1 hit.
[Graphical view]
PIRSFPIRSF016080. Restrict_endonuc_II_DpmII. 1 hit.
ProtoNetSearch...

Entry information

Entry nameT2L2_LACLC
AccessionPrimary (citable) accession number: P50177
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries