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Protein

Type-2 restriction enzyme LlaDCHI

Gene

llaDCHIR

Organism
Lactococcus lactis subsp. cremoris (Streptococcus cremoris)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Recognizes the double-stranded unmethylated sequence GATC and cleaves before G-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processRestriction system

Protein family/group databases

REBASEi2772 LlaDCHI

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme LlaDCHI (EC:3.1.21.4)
Short name:
R.LlaDCHI
Alternative name(s):
Endonuclease LlaDCHI
LlaII
Type II restriction enzyme LlaDCHI
Gene namesi
Name:llaDCHIR
Synonyms:llaIIC
Encoded oniPlasmid pSRQ7000 Publication
OrganismiLactococcus lactis subsp. cremoris (Streptococcus cremoris)
Taxonomic identifieri1359 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000773281 – 304Type-2 restriction enzyme LlaDCHIAdd BLAST304

Proteomic databases

PRIDEiP50177

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiView protein in InterPro
IPR021191 Restrct_endonuc_II_DpmII
IPR007637 Restrct_endonuc_II_DpmII-like
PfamiView protein in Pfam
PF04556 DpnII, 1 hit
PIRSFiPIRSF016080 Restrict_endonuc_II_DpmII, 1 hit

Sequencei

Sequence statusi: Complete.

P50177-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFNNYIGLE SDDRLNAFMA TLSVTNRTPE YYVNWEKVER ETRKFELELN
60 70 80 90 100
TLNYLIGKED IYSEALELFT NQPELLKAIP SLIASRDTSL DILNIDENDD
110 120 130 140 150
MSFEQLNFLV IDENCIADYV DFINQAGLLD FLQNKAKRSL VDYVYGVEAG
160 170 180 190 200
LDSNARKTRS GTTMEGILER TVSKIAQGKG LDWKPQATAS FIKSQWDIEV
210 220 230 240 250
PVDKSKRRFD AAVYSRALNK VWLIETNYYG GGGSKLKAVA GEFTELSQFV
260 270 280 290 300
KTSKDNVEFV WVTDGQGWKF SRLPLAEAFG HIDNVFNLTM LKEGFLSDLF

EKEI
Length:304
Mass (Da):34,670
Last modified:October 1, 1996 - v1
Checksum:iC0C84547A28A20E4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U16027 Genomic DNA Translation: AAB06313.1
RefSeqiNP_116731.1, NC_002798.1

Genome annotation databases

GeneIDi1113500

Similar proteinsi

Entry informationi

Entry nameiT2L2_LACLC
AccessioniPrimary (citable) accession number: P50177
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 57 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

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