P50177 (T2L2_LACLC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-2 restriction enzyme LlaDCHI
Short name=R.LlaDCHI
EC=3.1.21.4

Alternative name(s):
Endonuclease LlaDCHI
LlaII
Type II restriction enzyme LlaDCHI

Gene names

Name:	llaDCHIR
Synonyms:	llaIIC

Encoded on

Plasmid pSRQ700

Organism

Lactococcus lactis subsp. cremoris (Streptococcus cremoris)

Taxonomic identifier

1359 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	304 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Recognizes the double-stranded unmethylated sequence GATC and cleaves before G-1.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.
Sequence similarities	Belongs to the type II restriction enzyme DpnII family.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Plasmid
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 304	304	Type-2 restriction enzyme LlaDCHI		PRO_0000077328

Sequences

	Sequence		Length	Mass (Da)
	P50177 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: C0C84547A28A20E4	FASTA	304	34,670
	10 20 30 40 50 60 MDFNNYIGLE SDDRLNAFMA TLSVTNRTPE YYVNWEKVER ETRKFELELN TLNYLIGKED 70 80 90 100 110 120 IYSEALELFT NQPELLKAIP SLIASRDTSL DILNIDENDD MSFEQLNFLV IDENCIADYV 130 140 150 160 170 180 DFINQAGLLD FLQNKAKRSL VDYVYGVEAG LDSNARKTRS GTTMEGILER TVSKIAQGKG 190 200 210 220 230 240 LDWKPQATAS FIKSQWDIEV PVDKSKRRFD AAVYSRALNK VWLIETNYYG GGGSKLKAVA 250 260 270 280 290 300 GEFTELSQFV KTSKDNVEFV WVTDGQGWKF SRLPLAEAFG HIDNVFNLTM LKEGFLSDLF EKEI

References

[1]

"Cloning and sequencing of LlaDCHI restriction/modification genes from Lactococcus lactis and relatedness of this system to the Streptococcus pneumoniae DpnII system." Moineau S., Walker S.A., Vedamuthu E.R., Vandenbergh P.A. Appl. Environ. Microbiol. 61:2193-2202(1995) [PubMed: 7793939] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DCH-4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U16027 Genomic DNA. Translation: AAB06313.1.
RefSeq	NP_116731.1. NC_002798.1.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	2772. LlaDCHI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1113500.
Phylogenomic databases
ProtClustDB	CLSK2747636.
Family and domain databases
InterPro	IPR021191. Restrct_endonuc_II_DpmII. IPR007637. Restrct_endonuc_II_DpmII-like. [Graphical view]
Pfam	PF04556. DpnII. 1 hit. [Graphical view]
PIRSF	PIRSF016080. Restrict_endonuc_II_DpmII. 1 hit.
ProtoNet	Search...

Entry information

Entry name

T2L2_LACLC

Accession

Primary (citable) accession number: P50177

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	July 27, 2011
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program