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P50175 (PENK_MESAU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Proenkephalin-A

Cleaved into the following 8 chains:

  1. Synenkephalin
  2. Met-enkephalin
    Alternative name(s):
    Opioid growth factor
    Short name=OGF
  3. PENK(114-133)
  4. PENK(143-184)
  5. Met-enkephalin-Arg-Gly-Leu
  6. Leu-enkephalin
  7. PENK(238-259)
  8. Met-enkephalin-Arg-Phe
Gene names
Name:PENK
Synonyms:ENK
OrganismMesocricetus auratus (Golden hamster)
Taxonomic identifier10036 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeMesocricetus

Protein attributes

Sequence length268 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(238-259) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum.

Subcellular location

Secreted.

Post-translational modification

The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.

Sequence similarities

Belongs to the opioid neuropeptide precursor family.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionEndorphin
Neuropeptide
Opioid peptide
   PTMCleavage on pair of basic residues
Disulfide bond
Gene Ontology (GO)
   Biological_processneuropeptide signaling pathway

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Peptide25 – 9773Synenkephalin
PRO_0000008255
Peptide100 – 1045Met-enkephalin
PRO_0000008256
Peptide107 – 1115Met-enkephalin
PRO_0000008257
Peptide114 – 13320PENK(114-133) By similarity
PRO_0000377688
Peptide136 – 1405Met-enkephalin
PRO_0000008259
Peptide143 – 18442PENK(143-184) By similarity
PRO_0000377689
Peptide187 – 1948Met-enkephalin-Arg-Gly-Leu
PRO_0000008261
Propeptide197 – 20812
PRO_0000008262
Peptide211 – 2155Met-enkephalin
PRO_0000008263
Propeptide218 – 22811
PRO_0000008264
Peptide231 – 2355Leu-enkephalin
PRO_0000008265
Peptide238 – 25922PENK(238-259) By similarity
PRO_0000377690
Peptide262 – 2687Met-enkephalin-Arg-Phe
PRO_0000008267

Amino acid modifications

Disulfide bond26 ↔ 48 By similarity
Disulfide bond30 ↔ 52 By similarity
Disulfide bond33 ↔ 65 By similarity

Experimental info

Sequence conflict781C → W in AAB32115. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P50175 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 9670D1DEEA936725

FASTA26830,733
        10         20         30         40         50         60 
MARFLRLCTW LLVLGSCLLA TVQAECSQDC AKCSYHLVSP GDINFLACTL ECEGQMPSHK 

        70         80         90        100        110        120 
IWETCKDLLQ VSKPEFPCDS INMFKDSNKQ DESHLLAKKY GGFMKRYGGF MKKMDELYPV 

       130        140        150        160        170        180 
EPEEEANGGE ILAKKYGGFM KKDADEGDTL ANSSDLLKEL LGTGDNRARE GRHQESTDND 

       190        200        210        220        230        240 
DNMSKRYGGF MRGLKRSPQV EDEAKELQKR YGGFMRRVGR PEWWMDYQKR YGGFLKRFAE 

       250        260 
SLPSDEEAES YSKEVPEIEK RYGGFMRF 

« Hide

References

[1]"Cloning and characterization of hamster proenkephalin gene."
Zhu Y.S., Branch A.D., Robertson H.D., Inturrisi C.E.
DNA Cell Biol. 13:25-35(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Syrian.
Tissue: Liver.
[2]"Molecular cloning and characterization of the hamster preproenkephalin A cDNA."
Beaulieu M., Ouellette M., Desgroseillers L., Brakier-Gingras L.
DNA Cell Biol. 13:933-940(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Syrian.
Tissue: Adrenal gland.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U09941 Genomic DNA. Translation: AAA63650.1.
S74095 mRNA. Translation: AAB32115.1.
PIRI53029.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

HOVERGENHBG000063.

Family and domain databases

InterProIPR006024. Opioid_neupept.
IPR000703. Proenkphlin_A.
[Graphical view]
PANTHERPTHR11438. PTHR11438. 1 hit.
PTHR11438:SF3. PTHR11438:SF3. 1 hit.
PfamPF01160. Opiods_neuropep. 1 hit.
[Graphical view]
PRINTSPR01028. OPIOIDPRCRSR.
PR01029. PENKAPRCRSR.
PROSITEPS01252. OPIOIDS_PRECURSOR. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePENK_MESAU
AccessionPrimary (citable) accession number: P50175
Secondary accession number(s): Q64030
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 3, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families