P50175 (PENK_MESAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proenkephalin-A

Cleaved into the following 8 chains:

1. Synenkephalin
2. Met-enkephalin
   Alternative name(s):
   Opioid growth factor
   Short name=OGF
3. PENK(114-133)
4. PENK(143-184)
5. Met-enkephalin-Arg-Gly-Leu
6. Leu-enkephalin
7. PENK(238-259)
8. Met-enkephalin-Arg-Phe

Gene names

Name:	PENK
Synonyms:	ENK

Organism

Mesocricetus auratus (Golden hamster)

Taxonomic identifier

10036 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Mesocricetus

Protein attributes

Sequence length	268 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(238-259) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum.
Subcellular location	Secreted.
Post-translational modification	The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.
Sequence similarities	Belongs to the opioid neuropeptide precursor family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Endorphin Neuropeptide Opioid peptide
PTM	Cleavage on pair of basic residues Disulfide bond
Gene Ontology (GO)
Biological_process	neuropeptide signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Peptide	25 – 97	73	Synenkephalin		PRO_0000008255
	Peptide	100 – 104	5	Met-enkephalin		PRO_0000008256
	Peptide	107 – 111	5	Met-enkephalin		PRO_0000008257
	Peptide	114 – 133	20	PENK(114-133) By similarity		PRO_0000377688
	Peptide	136 – 140	5	Met-enkephalin		PRO_0000008259
	Peptide	143 – 184	42	PENK(143-184) By similarity		PRO_0000377689
	Peptide	187 – 194	8	Met-enkephalin-Arg-Gly-Leu		PRO_0000008261
	Propeptide	197 – 208	12			PRO_0000008262
	Peptide	211 – 215	5	Met-enkephalin		PRO_0000008263
	Propeptide	218 – 228	11			PRO_0000008264
	Peptide	231 – 235	5	Leu-enkephalin		PRO_0000008265
	Peptide	238 – 259	22	PENK(238-259) By similarity		PRO_0000377690
	Peptide	262 – 268	7	Met-enkephalin-Arg-Phe		PRO_0000008267
Amino acid modifications
	Disulfide bond	26 ↔ 48		By similarity
	Disulfide bond	30 ↔ 52		By similarity
	Disulfide bond	33 ↔ 65		By similarity
Experimental info
	Sequence conflict	78	1	C → W in AAB32115. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P50175 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 9670D1DEEA936725	FASTA	268	30,733
	10 20 30 40 50 60 MARFLRLCTW LLVLGSCLLA TVQAECSQDC AKCSYHLVSP GDINFLACTL ECEGQMPSHK 70 80 90 100 110 120 IWETCKDLLQ VSKPEFPCDS INMFKDSNKQ DESHLLAKKY GGFMKRYGGF MKKMDELYPV 130 140 150 160 170 180 EPEEEANGGE ILAKKYGGFM KKDADEGDTL ANSSDLLKEL LGTGDNRARE GRHQESTDND 190 200 210 220 230 240 DNMSKRYGGF MRGLKRSPQV EDEAKELQKR YGGFMRRVGR PEWWMDYQKR YGGFLKRFAE 250 260 SLPSDEEAES YSKEVPEIEK RYGGFMRF

References

[1]	"Cloning and characterization of hamster proenkephalin gene." Zhu Y.S., Branch A.D., Robertson H.D., Inturrisi C.E. DNA Cell Biol. 13:25-35(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Syrian. Tissue: Liver.
[2]	"Molecular cloning and characterization of the hamster preproenkephalin A cDNA." Beaulieu M., Ouellette M., Desgroseillers L., Brakier-Gingras L. DNA Cell Biol. 13:933-940(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Syrian. Tissue: Adrenal gland.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U09941 Genomic DNA. Translation: AAA63650.1. S74095 mRNA. Translation: AAB32115.1.
PIR	I53029.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG000063.
Family and domain databases
InterPro	IPR006024. Opioid_neupept. IPR000703. Proenkphlin_A. [Graphical view]
PANTHER	PTHR11438. PTHR11438. 1 hit. PTHR11438:SF3. PTHR11438:SF3. 1 hit.
Pfam	PF01160. Opiods_neuropep. 1 hit. [Graphical view]
PRINTS	PR01028. OPIOIDPRCRSR. PR01029. PENKAPRCRSR.
PROSITE	PS01252. OPIOIDS_PRECURSOR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PENK_MESAU

Accession

Primary (citable) accession number: P50175
Secondary accession number(s): Q64030

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program