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Protein

Proenkephalin-A

Gene

PENK

Organism
Mesocricetus auratus (Golden hamster)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(238-259) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum.

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endorphin, Neuropeptide, Opioid peptide

Names & Taxonomyi

Protein namesi
Recommended name:
Proenkephalin-A
Cleaved into the following 8 chains:
Gene namesi
Name:PENK
Synonyms:ENK
OrganismiMesocricetus auratus (Golden hamster)
Taxonomic identifieri10036 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeMesocricetus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
PeptideiPRO_000000825525 – 97SynenkephalinAdd BLAST73
PeptideiPRO_0000008256100 – 104Met-enkephalin5
PeptideiPRO_0000008257107 – 111Met-enkephalin5
PeptideiPRO_0000377688114 – 133PENK(114-133)By similarityAdd BLAST20
PeptideiPRO_0000008259136 – 140Met-enkephalin5
PeptideiPRO_0000377689143 – 184PENK(143-184)By similarityAdd BLAST42
PeptideiPRO_0000008261187 – 194Met-enkephalin-Arg-Gly-Leu8
PropeptideiPRO_0000008262197 – 208Add BLAST12
PeptideiPRO_0000008263211 – 215Met-enkephalin5
PropeptideiPRO_0000008264218 – 228Add BLAST11
PeptideiPRO_0000008265231 – 235Leu-enkephalin5
PeptideiPRO_0000377690238 – 259PENK(238-259)By similarityAdd BLAST22
PeptideiPRO_0000008267262 – 268Met-enkephalin-Arg-Phe7

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi26 ↔ 48By similarity
Disulfide bondi30 ↔ 52By similarity
Disulfide bondi33 ↔ 65By similarity
Modified residuei252PhosphoserineBy similarity1

Post-translational modificationi

The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Phosphoprotein

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG000063.

Family and domain databases

InterProiIPR006024. Opioid_neupept.
IPR000703. Proenkphlin_A.
[Graphical view]
PANTHERiPTHR11438. PTHR11438. 1 hit.
PTHR11438:SF3. PTHR11438:SF3. 1 hit.
PfamiPF01160. Opiods_neuropep. 1 hit.
[Graphical view]
PRINTSiPR01028. OPIOIDPRCRSR.
PR01029. PENKAPRCRSR.
PROSITEiPS01252. OPIOIDS_PRECURSOR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50175-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARFLRLCTW LLVLGSCLLA TVQAECSQDC AKCSYHLVSP GDINFLACTL
60 70 80 90 100
ECEGQMPSHK IWETCKDLLQ VSKPEFPCDS INMFKDSNKQ DESHLLAKKY
110 120 130 140 150
GGFMKRYGGF MKKMDELYPV EPEEEANGGE ILAKKYGGFM KKDADEGDTL
160 170 180 190 200
ANSSDLLKEL LGTGDNRARE GRHQESTDND DNMSKRYGGF MRGLKRSPQV
210 220 230 240 250
EDEAKELQKR YGGFMRRVGR PEWWMDYQKR YGGFLKRFAE SLPSDEEAES
260
YSKEVPEIEK RYGGFMRF
Length:268
Mass (Da):30,733
Last modified:October 1, 1996 - v1
Checksum:i9670D1DEEA936725
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti78C → W in AAB32115 (PubMed:7917015).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09941 Genomic DNA. Translation: AAA63650.1.
S74095 mRNA. Translation: AAB32115.1.
PIRiI53029.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09941 Genomic DNA. Translation: AAA63650.1.
S74095 mRNA. Translation: AAB32115.1.
PIRiI53029.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG000063.

Family and domain databases

InterProiIPR006024. Opioid_neupept.
IPR000703. Proenkphlin_A.
[Graphical view]
PANTHERiPTHR11438. PTHR11438. 1 hit.
PTHR11438:SF3. PTHR11438:SF3. 1 hit.
PfamiPF01160. Opiods_neuropep. 1 hit.
[Graphical view]
PRINTSiPR01028. OPIOIDPRCRSR.
PR01029. PENKAPRCRSR.
PROSITEiPS01252. OPIOIDS_PRECURSOR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPENK_MESAU
AccessioniPrimary (citable) accession number: P50175
Secondary accession number(s): Q64030
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 14, 2015
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.