Skip Header

Contribute Send feedback
Read comments (?) or add your own

P50173 (GLDA_PSEPU) Reviewed, UniProtKB/Swiss-Prot

Last modified June 28, 2011. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glycerol dehydrogenase
Short name=GDH
Short name=GLDH
EC=1.1.1.6
Gene names
Name:gldA
Synonyms:bedD
Encoded onPlasmid pHMT112
OrganismPseudomonas putida (Arthrobacter siderocapsulatus)
Taxonomic identifier303 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length365 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the NAD-dependent oxidation of glycerol to dihydroxyacetone (glycerone). Allows microorganisms to utilize glycerol as a source of carbon under anaerobic conditions By similarity.

Catalytic activity

Glycerol + NAD+ = glycerone + NADH.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Pathway

Polyol metabolism; glycerol fermentation; glycerone phosphate from glycerol (oxidative route): step 1/2.

Sequence similarities

Belongs to the iron-containing alcohol dehydrogenase family.

Ontologies

Keywords
   Biological processGlycerol metabolism
   LigandMetal-binding
NAD
Zinc
   Molecular functionOxidoreductase
   Technical termPlasmid
Gene Ontology (GO)
   Biological processglycerol metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionglycerol dehydrogenase activity

Inferred from electronic annotation. Source: EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 365365Glycerol dehydrogenase
PRO_0000087830

Regions

Nucleotide binding94 – 985NAD By similarity
Nucleotide binding116 – 1194NAD By similarity

Sites

Metal binding1711Zinc; catalytic By similarity
Metal binding2541Zinc; catalytic By similarity
Metal binding2711Zinc; catalytic By similarity
Binding site371NAD By similarity
Binding site1211Substrate By similarity
Binding site1251NAD By similarity
Binding site1271NAD; via carbonyl oxygen By similarity
Binding site1311NAD By similarity
Binding site1711Substrate By similarity
Binding site2541Substrate By similarity
Binding site2711Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
P50173 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: D6AEAA16D52A8D47

FASTA36538,316
        10         20         30         40         50         60 
MDRAIQSPGK YVQGADALQR LGDYLKPLAD SWLVIADKFV LGFAEDTIRQ SLSKAGLAMD 

        70         80         90        100        110        120 
IVAFNGECSQ GEVDRLCQLA TQNGRSAIVG IGGGKTLDTA KAVAFFQKVP VAVAPTIAST 

       130        140        150        160        170        180 
DAPCSALSVL YTDEGEFDRY LMLPTNPALV VVDTAIVARA PARLLAAGIG DALATWFEAR 

       190        200        210        220        230        240 
AASRSSAATM AGGPATQTAL NLARFCYDTL LEEGEKAMLA VQAQVVTPAL ERIVEANTYL 

       250        260        270        280        290        300 
SGVGFESGGV AAAHAVHNGL TAVAETHHFY HGEKVAFGVL VQLALENASN AEMQEVMSLC 

       310        320        330        340        350        360 
HAVGLPITLA QLDITEDIPT KMRAVAELAC APGETIHNMP GGVTVEQVYG ALLVADQLGQ 


HFLEF

« Hide

References

[1]"Characterization and expression of the plasmid-borne bedD gene from Pseudomonas putida ML2, which codes for a NAD+-dependent cis-benzene dihydrodiol dehydrogenase."
Fong K.P.Y., Goh C.B.H., Tan H.M.
J. Bacteriol. 178:5592-5601(1996) [PubMed: 8824602] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ML2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF148496 Genomic DNA. Translation: AAC44426.1.

3D structure databases

ProteinModelPortalP50173.
SMRP50173. Positions 1-364.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR001670. ADH_Fe.
IPR018211. ADH_Fe_CS.
IPR016205. Glycerol_DH.
[Graphical view]
PfamPF00465. Fe-ADH. 1 hit.
[Graphical view]
PIRSFPIRSF000112. Glycerol_dehydrogenase. 1 hit.
PROSITEPS00913. ADH_IRON_1. 1 hit.
PS00060. ADH_IRON_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLDA_PSEPU
AccessionPrimary (citable) accession number: P50173
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 28, 2011
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents