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P50172

- DHI1_MOUSE

UniProt

P50172 - DHI1_MOUSE

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Protein
Corticosteroid 11-beta-dehydrogenase isozyme 1
Gene
Hsd11b1, Hsd11
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone.

Catalytic activityi

An 11-beta-hydroxysteroid + NADP+ = an 11-oxosteroid + NADPH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei170 – 1701Substrate
Active sitei183 – 1831Proton acceptor

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi41 – 6727NADP
Add
BLAST
Nucleotide bindingi92 – 932NADP
Nucleotide bindingi119 – 1213NADP
Nucleotide bindingi183 – 1875NADP
Nucleotide bindingi216 – 2227NADP

GO - Molecular functioni

  1. 11-beta-hydroxysteroid dehydrogenase (NADP+) activity Source: UniProtKB-EC
  2. 11-beta-hydroxysteroid dehydrogenase [NAD(P)] activity Source: MGI
Complete GO annotation...

GO - Biological processi

  1. lung development Source: MGI
  2. steroid metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lipid metabolism, Steroid metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

BRENDAi1.1.1.146. 3474.
ReactomeiREACT_207160. Glucocorticoid biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Corticosteroid 11-beta-dehydrogenase isozyme 1 (EC:1.1.1.146)
Alternative name(s):
11-beta-hydroxysteroid dehydrogenase 1
Short name:
11-DH
Short name:
11-beta-HSD1
11beta-HSD1A
Gene namesi
Name:Hsd11b1
Synonyms:Hsd11
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:103562. Hsd11b1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 76Cytoplasmic Reviewed prediction
Transmembranei8 – 2417Helical; Signal-anchor for type II membrane protein; Reviewed prediction
Add
BLAST
Topological domaini25 – 292268Lumenal Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 292291Corticosteroid 11-beta-dehydrogenase isozyme 1
PRO_0000054621Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi162 – 1621N-linked (GlcNAc...) Reviewed prediction
Glycosylationi207 – 2071N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP50172.
PaxDbiP50172.
PRIDEiP50172.

PTM databases

PhosphoSiteiP50172.

Expressioni

Tissue specificityi

Widely expressed. Highest expression in liver.

Gene expression databases

ArrayExpressiP50172.
BgeeiP50172.
CleanExiMM_HSD11B1.
GenevestigatoriP50172.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

IntActiP50172. 7 interactions.
MINTiMINT-1863132.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi29 – 324
Beta strandi36 – 416
Helixi45 – 5612
Beta strandi60 – 634
Helixi68 – 8114
Beta strandi84 – 885
Helixi96 – 11015
Beta strandi114 – 1185
Helixi133 – 14311
Helixi145 – 16117
Beta strandi164 – 1707
Helixi171 – 1733
Helixi181 – 20323
Beta strandi209 – 2157
Helixi221 – 2277
Turni228 – 2303
Helixi238 – 25013
Beta strandi254 – 2585
Helixi264 – 2685
Helixi271 – 28010
Helixi281 – 2833
Helixi286 – 2883

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Y5MX-ray2.30A/B24-292[»]
1Y5RX-ray3.00A/B24-292[»]
3GMDX-ray2.28A/B/C/D/E/F/G/H26-289[»]
4K26X-ray2.21A/B24-292[»]
4NMHX-ray2.90A/B/C/D24-292[»]
ProteinModelPortaliP50172.
SMRiP50172. Positions 25-289.

Miscellaneous databases

EvolutionaryTraceiP50172.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1028.
HOGENOMiHOG000010276.
HOVERGENiHBG005481.
InParanoidiP50172.
KOiK15680.
OMAiWGGLDML.
OrthoDBiEOG7353X9.
PhylomeDBiP50172.
TreeFamiTF329114.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50172-1 [UniParc]FASTAAdd to Basket

« Hide

MAVMKNYLLP ILVLFLAYYY YSTNEEFRPE MLQGKKVIVT GASKGIGREM    50
AYHLSKMGAH VVLTARSEEG LQKVVSRCLE LGAASAHYIA GTMEDMTFAE 100
QFIVKAGKLM GGLDMLILNH ITQTSLSLFH DDIHSVRRVM EVNFLSYVVM 150
STAALPMLKQ SNGSIAVISS LAGKMTQPMI APYSASKFAL DGFFSTIRTE 200
LYITKVNVSI TLCVLGLIDT ETAMKEISGI INAQASPKEE CALEIIKGTA 250
LRKSEVYYDK SPLTPILLGN PGRKIMEFFS LRYYNKDMFV SN 292
Length:292
Mass (Da):32,364
Last modified:January 23, 2007 - v3
Checksum:iADE42B11D82DD6CD
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti15 – 151F → S in CAA58209. 1 Publication
Sequence conflicti232 – 2321N → D in CAA58209. 1 Publication
Sequence conflicti234 – 2341Q → L in CAA58209. 1 Publication
Sequence conflicti261 – 2611S → L in CAA58209. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S75207 mRNA. Translation: AAB33601.1.
X83202 mRNA. Translation: CAA58209.1.
X92186 Genomic DNA. Translation: CAA63096.1.
CCDSiCCDS15635.1.
PIRiI56604.
RefSeqiNP_001038216.1. NM_001044751.1.
NP_032314.2. NM_008288.2.
UniGeneiMm.28328.

Genome annotation databases

EnsembliENSMUST00000016338; ENSMUSP00000016338; ENSMUSG00000016194.
ENSMUST00000161737; ENSMUSP00000125620; ENSMUSG00000016194.
GeneIDi15483.
KEGGimmu:15483.
UCSCiuc007eef.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S75207 mRNA. Translation: AAB33601.1 .
X83202 mRNA. Translation: CAA58209.1 .
X92186 Genomic DNA. Translation: CAA63096.1 .
CCDSi CCDS15635.1.
PIRi I56604.
RefSeqi NP_001038216.1. NM_001044751.1.
NP_032314.2. NM_008288.2.
UniGenei Mm.28328.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1Y5M X-ray 2.30 A/B 24-292 [» ]
1Y5R X-ray 3.00 A/B 24-292 [» ]
3GMD X-ray 2.28 A/B/C/D/E/F/G/H 26-289 [» ]
4K26 X-ray 2.21 A/B 24-292 [» ]
4NMH X-ray 2.90 A/B/C/D 24-292 [» ]
ProteinModelPortali P50172.
SMRi P50172. Positions 25-289.
ModBasei Search...

Protein-protein interaction databases

IntActi P50172. 7 interactions.
MINTi MINT-1863132.

Chemistry

BindingDBi P50172.
ChEMBLi CHEMBL3910.

PTM databases

PhosphoSitei P50172.

Proteomic databases

MaxQBi P50172.
PaxDbi P50172.
PRIDEi P50172.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000016338 ; ENSMUSP00000016338 ; ENSMUSG00000016194 .
ENSMUST00000161737 ; ENSMUSP00000125620 ; ENSMUSG00000016194 .
GeneIDi 15483.
KEGGi mmu:15483.
UCSCi uc007eef.1. mouse.

Organism-specific databases

CTDi 3290.
MGIi MGI:103562. Hsd11b1.

Phylogenomic databases

eggNOGi COG1028.
HOGENOMi HOG000010276.
HOVERGENi HBG005481.
InParanoidi P50172.
KOi K15680.
OMAi WGGLDML.
OrthoDBi EOG7353X9.
PhylomeDBi P50172.
TreeFami TF329114.

Enzyme and pathway databases

BRENDAi 1.1.1.146. 3474.
Reactomei REACT_207160. Glucocorticoid biosynthesis.

Miscellaneous databases

EvolutionaryTracei P50172.
NextBioi 288336.
PROi P50172.
SOURCEi Search...

Gene expression databases

ArrayExpressi P50172.
Bgeei P50172.
CleanExi MM_HSD11B1.
Genevestigatori P50172.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view ]
Pfami PF00106. adh_short. 1 hit.
[Graphical view ]
PRINTSi PR00081. GDHRDH.
PROSITEi PS00061. ADH_SHORT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning, sequencing and tissue-distribution of mouse 11 beta-hydroxysteroid dehydrogenase-1 cDNA."
    Rajan V., Chapman K.E., Lyons V., Jamieson P., Mullins J.J., Edwards C.R., Seckl J.R.
    J. Steroid Biochem. Mol. Biol. 52:141-147(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6 X CBA.
    Tissue: Liver.
  2. "Cloning and primary structure of murine 11 beta-hydroxysteroid dehydrogenase/microsomal carbonyl reductase."
    Oppermann U.C.T., Netter K.J., Maser E.
    Eur. J. Biochem. 227:202-208(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Liver.
  3. "The sequence of 5' flanking DNA from the mouse 11 beta-hydroxysteroid dehydrogenase type 1 gene and analysis of putative transcription factor binding sites."
    Voice M.W., Seckl J.R., Chapman K.E.
    Gene 181:233-235(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-10.
  4. "Crystal structure of murine 11 beta-hydroxysteroid dehydrogenase 1: an important therapeutic target for diabetes."
    Zhang J., Osslund T.D., Plant M.H., Clogston C.L., Nybo R.E., Xiong F., Delaney J.M., Jordan S.R.
    Biochemistry 44:6948-6957(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 24-291 IN COMPLEXES WITH NADP AND CORTICOSTERONE, SUBUNIT.

Entry informationi

Entry nameiDHI1_MOUSE
AccessioniPrimary (citable) accession number: P50172
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 125 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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