Reviewed, UniProtKB/Swiss-Prot P50172 (DHI1_MOUSE)

Names and origin

Protein names

Recommended name:
    Corticosteroid 11-beta-dehydrogenase isozyme 1
    EC=1.1.1.146
Alternative name(s):
    11-beta-hydroxysteroid dehydrogenase 1
      Short name=11-beta-HSD1
    11beta-HSD1A
    11-DH

Gene names

Name:	Hsd11b1
Synonyms:	Hsd11

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone.
Catalytic activity	An 11-beta-hydroxysteroid + NADP+ = an 11-oxosteroid + NADPH.
Subunit structure	Homodimer By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein.
Tissue specificity	Widely expressed. Highest expression in liver.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	Lipid metabolism Steroid metabolism
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal-anchor Transmembrane
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Glycoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	lung development Inferred from mutant phenotype. Source: MGI oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW steroid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	11-beta-hydroxysteroid dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed By similarity

Chain

2 – 292

291

Corticosteroid 11-beta-dehydrogenase isozyme 1

PRO_0000054621

Regions

Topological domain

2 – 7

6

Cytoplasmic Potential

Transmembrane

8 – 24

17

Signal-anchor for type II membrane protein Potential

Topological domain

25 – 292

268

Lumenal Potential

Nucleotide binding

34 – 63

30

NADP By similarity

Sites

Active site

183

1

Proton acceptor By similarity

Binding site

170

1

Substrate By similarity

Amino acid modifications

Glycosylation

162

1

N-linked (GlcNAc...) Potential

Glycosylation

207

1

N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict

15

1

F → S in CAA58209. Ref.2

Sequence conflict

232

1

N → D Ref.2

Sequence conflict

234

1

Q → L Ref.2

Sequence conflict

261

1

S → L in CAA58209. Ref.2

Secondary structure

1

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292

Helix Strand Turn

Details...

Helix

29 – 32

4

Beta strand

36 – 41

6

Helix

45 – 56

12

Beta strand

60 – 66

7

Helix

68 – 81

14

Beta strand

84 – 90

7

Helix

96 – 110

15

Beta strand

114 – 118

5

Helix

133 – 143

11

Helix

145 – 161

17

Beta strand

164 – 170

7

Helix

171 – 173

3

Helix

181 – 203

23

Beta strand

209 – 215

7

Helix

221 – 227

7

Turn

228 – 230

3

Helix

238 – 250

13

Beta strand

254 – 258

5

Helix

263 – 268

6

Helix

271 – 280

10

Helix

281 – 283

3

Helix

286 – 288

3

Sequences

	Sequence		Length	Mass (Da)
	P50172-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: ADE42B11D82DD6CD	FASTA	292	32,364
	10 20 30 40 50 60 MAVMKNYLLP ILVLFLAYYY YSTNEEFRPE MLQGKKVIVT GASKGIGREM AYHLSKMGAH 70 80 90 100 110 120 VVLTARSEEG LQKVVSRCLE LGAASAHYIA GTMEDMTFAE QFIVKAGKLM GGLDMLILNH 130 140 150 160 170 180 ITQTSLSLFH DDIHSVRRVM EVNFLSYVVM STAALPMLKQ SNGSIAVISS LAGKMTQPMI 190 200 210 220 230 240 APYSASKFAL DGFFSTIRTE LYITKVNVSI TLCVLGLIDT ETAMKEISGI INAQASPKEE 250 260 270 280 290 CALEIIKGTA LRKSEVYYDK SPLTPILLGN PGRKIMEFFS LRYYNKDMFV SN

References

[1]	"Cloning, sequencing and tissue-distribution of mouse 11 beta-hydroxysteroid dehydrogenase-1 cDNA." Rajan V., Chapman K.E., Lyons V., Jamieson P., Mullins J.J., Edwards C.R., Seckl J.R. J. Steroid Biochem. Mol. Biol. 52:141-147(1995) [PubMed: 7873449] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6 X CBA. Tissue: Liver.
[2]	"Cloning and primary structure of murine 11 beta-hydroxysteroid dehydrogenase/microsomal carbonyl reductase." Oppermann U.C.T., Netter K.J., Maser E. Eur. J. Biochem. 227:202-208(1995) [PubMed: 7851387] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Liver.
[3]	"The sequence of 5' flanking DNA from the mouse 11 beta-hydroxysteroid dehydrogenase type 1 gene and analysis of putative transcription factor binding sites." Voice M.W., Seckl J.R., Chapman K.E. Gene 181:233-235(1996) [PubMed: 8973338] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-10.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

S75207 mRNA. Translation: AAB33601.1.
X83202 mRNA. Translation: CAA58209.1.
X92186 Genomic DNA. Translation: CAA63096.1.

IPI

IPI00115599.

PIR

I56604.

RefSeq

NP_001038216.1.
NP_032314.2.

UniGene

Mm.28328

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1Y5M	X-ray	2.30	A/B	24-291	[»]
1Y5R	X-ray	3.00	A/B	24-290	[»]

ModBase

Search...

Proteomic databases

PRIDE

P50172.

Genome annotation databases

Ensembl

ENSMUSG00000016194. Mus musculus. [Contig view]

GeneID

15483.

KEGG

mmu:15483.

Organism-specific databases

MGI

MGI:103562. Hsd11b1.

Phylogenomic databases

HOGENOM

P50172.

HOVERGEN

P50172.

OMA

P50172. SSIRTEY.

Enzyme and pathway databases

BRENDA

1.1.1.146. 244.

Gene expression databases

ArrayExpress

P50172.

Bgee

P50172.

CleanEx

MM_HSD11B1.

GermOnline

ENSMUSG00000016194. Mus musculus.

Family and domain databases

InterPro

IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]

Gene3D

G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.

PANTHER

PTHR19410. ADH_short_C2. 1 hit.

Pfam

PF00106. adh_short. 1 hit.
[Graphical view]

PRINTS

PR00081. GDHRDH.

PROSITE

PS00061. ADH_SHORT. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

BindingDB

P50172.

NextBio

288336.

SOURCE

Search...

Entry information

Entry name

DHI1_MOUSE

Accession

Primary (citable) accession number: P50172

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 80 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)