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P50172 (DHI1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Corticosteroid 11-beta-dehydrogenase isozyme 1

EC=1.1.1.146
Alternative name(s):
11-beta-hydroxysteroid dehydrogenase 1
Short name=11-DH
Short name=11-beta-HSD1
11beta-HSD1A
Gene names
Name:Hsd11b1
Synonyms:Hsd11
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length292 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone.

Catalytic activity

An 11-beta-hydroxysteroid + NADP+ = an 11-oxosteroid + NADPH.

Subunit structure

Homodimer. Ref.4

Subcellular location

Endoplasmic reticulum membrane; Single-pass type II membrane protein.

Tissue specificity

Widely expressed. Highest expression in liver.

Sequence similarities

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 292291Corticosteroid 11-beta-dehydrogenase isozyme 1
PRO_0000054621

Regions

Topological domain2 – 76Cytoplasmic Potential
Transmembrane8 – 2417Helical; Signal-anchor for type II membrane protein; Potential
Topological domain25 – 292268Lumenal Potential
Nucleotide binding41 – 6727NADP
Nucleotide binding92 – 932NADP
Nucleotide binding119 – 1213NADP
Nucleotide binding183 – 1875NADP
Nucleotide binding216 – 2227NADP

Sites

Active site1831Proton acceptor
Binding site1701Substrate

Amino acid modifications

Glycosylation1621N-linked (GlcNAc...) Potential
Glycosylation2071N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict151F → S in CAA58209. Ref.2
Sequence conflict2321N → D in CAA58209. Ref.2
Sequence conflict2341Q → L in CAA58209. Ref.2
Sequence conflict2611S → L in CAA58209. Ref.2

Secondary structure

.......................................... 292
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P50172 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: ADE42B11D82DD6CD

FASTA29232,364
        10         20         30         40         50         60 
MAVMKNYLLP ILVLFLAYYY YSTNEEFRPE MLQGKKVIVT GASKGIGREM AYHLSKMGAH 

        70         80         90        100        110        120 
VVLTARSEEG LQKVVSRCLE LGAASAHYIA GTMEDMTFAE QFIVKAGKLM GGLDMLILNH 

       130        140        150        160        170        180 
ITQTSLSLFH DDIHSVRRVM EVNFLSYVVM STAALPMLKQ SNGSIAVISS LAGKMTQPMI 

       190        200        210        220        230        240 
APYSASKFAL DGFFSTIRTE LYITKVNVSI TLCVLGLIDT ETAMKEISGI INAQASPKEE 

       250        260        270        280        290 
CALEIIKGTA LRKSEVYYDK SPLTPILLGN PGRKIMEFFS LRYYNKDMFV SN 

« Hide

References

[1]"Cloning, sequencing and tissue-distribution of mouse 11 beta-hydroxysteroid dehydrogenase-1 cDNA."
Rajan V., Chapman K.E., Lyons V., Jamieson P., Mullins J.J., Edwards C.R., Seckl J.R.
J. Steroid Biochem. Mol. Biol. 52:141-147(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6 X CBA.
Tissue: Liver.
[2]"Cloning and primary structure of murine 11 beta-hydroxysteroid dehydrogenase/microsomal carbonyl reductase."
Oppermann U.C.T., Netter K.J., Maser E.
Eur. J. Biochem. 227:202-208(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
Tissue: Liver.
[3]"The sequence of 5' flanking DNA from the mouse 11 beta-hydroxysteroid dehydrogenase type 1 gene and analysis of putative transcription factor binding sites."
Voice M.W., Seckl J.R., Chapman K.E.
Gene 181:233-235(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-10.
[4]"Crystal structure of murine 11 beta-hydroxysteroid dehydrogenase 1: an important therapeutic target for diabetes."
Zhang J., Osslund T.D., Plant M.H., Clogston C.L., Nybo R.E., Xiong F., Delaney J.M., Jordan S.R.
Biochemistry 44:6948-6957(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 24-291 IN COMPLEXES WITH NADP AND CORTICOSTERONE, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S75207 mRNA. Translation: AAB33601.1.
X83202 mRNA. Translation: CAA58209.1.
X92186 Genomic DNA. Translation: CAA63096.1.
PIRI56604.
RefSeqNP_001038216.1. NM_001044751.1.
NP_032314.2. NM_008288.2.
UniGeneMm.28328.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1Y5MX-ray2.30A/B24-291[»]
1Y5RX-ray3.00A/B24-290[»]
3GMDX-ray2.28A/B/C/D/E/F/G/H26-289[»]
ProteinModelPortalP50172.
SMRP50172. Positions 25-289.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP50172. 7 interactions.
MINTMINT-1863132.

Chemistry

BindingDBP50172.
ChEMBLCHEMBL3910.

PTM databases

PhosphoSiteP50172.

Proteomic databases

PaxDbP50172.
PRIDEP50172.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000016338; ENSMUSP00000016338; ENSMUSG00000016194.
ENSMUST00000161737; ENSMUSP00000125620; ENSMUSG00000016194.
GeneID15483.
KEGGmmu:15483.
UCSCuc007eef.1. mouse.

Organism-specific databases

CTD3290.
MGIMGI:103562. Hsd11b1.

Phylogenomic databases

eggNOGCOG1028.
HOGENOMHOG000010276.
HOVERGENHBG005481.
InParanoidP50172.
KOK15680.
OMAGLFMAYY.
OrthoDBEOG7353X9.
PhylomeDBP50172.
TreeFamTF329114.

Enzyme and pathway databases

BRENDA1.1.1.146. 3474.

Gene expression databases

ArrayExpressP50172.
BgeeP50172.
CleanExMM_HSD11B1.
GenevestigatorP50172.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSPR00081. GDHRDH.
PROSITEPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP50172.
NextBio288336.
PROP50172.
SOURCESearch...

Entry information

Entry nameDHI1_MOUSE
AccessionPrimary (citable) accession number: P50172
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 121 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot