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P50172

- DHI1_MOUSE

UniProt

P50172 - DHI1_MOUSE

Protein

Corticosteroid 11-beta-dehydrogenase isozyme 1

Gene

Hsd11b1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone.

    Catalytic activityi

    An 11-beta-hydroxysteroid + NADP+ = an 11-oxosteroid + NADPH.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei170 – 1701Substrate
    Active sitei183 – 1831Proton acceptor

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi41 – 6727NADPAdd
    BLAST
    Nucleotide bindingi92 – 932NADP
    Nucleotide bindingi119 – 1213NADP
    Nucleotide bindingi183 – 1875NADP
    Nucleotide bindingi216 – 2227NADP

    GO - Molecular functioni

    1. 11-beta-hydroxysteroid dehydrogenase (NADP+) activity Source: UniProtKB-EC
    2. 11-beta-hydroxysteroid dehydrogenase [NAD(P)] activity Source: MGI

    GO - Biological processi

    1. lung development Source: MGI
    2. steroid metabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Lipid metabolism, Steroid metabolism

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BRENDAi1.1.1.146. 3474.
    ReactomeiREACT_207160. Glucocorticoid biosynthesis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Corticosteroid 11-beta-dehydrogenase isozyme 1 (EC:1.1.1.146)
    Alternative name(s):
    11-beta-hydroxysteroid dehydrogenase 1
    Short name:
    11-DH
    Short name:
    11-beta-HSD1
    11beta-HSD1A
    Gene namesi
    Name:Hsd11b1
    Synonyms:Hsd11
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:103562. Hsd11b1.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 292291Corticosteroid 11-beta-dehydrogenase isozyme 1PRO_0000054621Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi162 – 1621N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi207 – 2071N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiP50172.
    PaxDbiP50172.
    PRIDEiP50172.

    PTM databases

    PhosphoSiteiP50172.

    Expressioni

    Tissue specificityi

    Widely expressed. Highest expression in liver.

    Gene expression databases

    ArrayExpressiP50172.
    BgeeiP50172.
    CleanExiMM_HSD11B1.
    GenevestigatoriP50172.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    IntActiP50172. 7 interactions.
    MINTiMINT-1863132.

    Structurei

    Secondary structure

    1
    292
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi29 – 324
    Beta strandi36 – 416
    Helixi45 – 5612
    Beta strandi60 – 634
    Helixi68 – 8114
    Beta strandi84 – 885
    Helixi96 – 11015
    Beta strandi114 – 1185
    Helixi133 – 14311
    Helixi145 – 16117
    Beta strandi164 – 1707
    Helixi171 – 1733
    Helixi181 – 20323
    Beta strandi209 – 2157
    Helixi221 – 2277
    Turni228 – 2303
    Helixi238 – 25013
    Beta strandi254 – 2585
    Helixi264 – 2685
    Helixi271 – 28010
    Helixi281 – 2833
    Helixi286 – 2883

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1Y5MX-ray2.30A/B24-292[»]
    1Y5RX-ray3.00A/B24-292[»]
    3GMDX-ray2.28A/B/C/D/E/F/G/H26-289[»]
    4K26X-ray2.21A/B24-292[»]
    4NMHX-ray2.90A/B/C/D24-292[»]
    ProteinModelPortaliP50172.
    SMRiP50172. Positions 25-289.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP50172.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini2 – 76CytoplasmicSequence Analysis
    Topological domaini25 – 292268LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei8 – 2417Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1028.
    HOGENOMiHOG000010276.
    HOVERGENiHBG005481.
    InParanoidiP50172.
    KOiK15680.
    OMAiWGGLDML.
    OrthoDBiEOG7353X9.
    PhylomeDBiP50172.
    TreeFamiTF329114.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    [Graphical view]
    PfamiPF00106. adh_short. 1 hit.
    [Graphical view]
    PRINTSiPR00081. GDHRDH.
    PROSITEiPS00061. ADH_SHORT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P50172-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAVMKNYLLP ILVLFLAYYY YSTNEEFRPE MLQGKKVIVT GASKGIGREM    50
    AYHLSKMGAH VVLTARSEEG LQKVVSRCLE LGAASAHYIA GTMEDMTFAE 100
    QFIVKAGKLM GGLDMLILNH ITQTSLSLFH DDIHSVRRVM EVNFLSYVVM 150
    STAALPMLKQ SNGSIAVISS LAGKMTQPMI APYSASKFAL DGFFSTIRTE 200
    LYITKVNVSI TLCVLGLIDT ETAMKEISGI INAQASPKEE CALEIIKGTA 250
    LRKSEVYYDK SPLTPILLGN PGRKIMEFFS LRYYNKDMFV SN 292
    Length:292
    Mass (Da):32,364
    Last modified:January 23, 2007 - v3
    Checksum:iADE42B11D82DD6CD
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti15 – 151F → S in CAA58209. (PubMed:7851387)Curated
    Sequence conflicti232 – 2321N → D in CAA58209. (PubMed:7851387)Curated
    Sequence conflicti234 – 2341Q → L in CAA58209. (PubMed:7851387)Curated
    Sequence conflicti261 – 2611S → L in CAA58209. (PubMed:7851387)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S75207 mRNA. Translation: AAB33601.1.
    X83202 mRNA. Translation: CAA58209.1.
    X92186 Genomic DNA. Translation: CAA63096.1.
    CCDSiCCDS15635.1.
    PIRiI56604.
    RefSeqiNP_001038216.1. NM_001044751.1.
    NP_032314.2. NM_008288.2.
    UniGeneiMm.28328.

    Genome annotation databases

    EnsembliENSMUST00000016338; ENSMUSP00000016338; ENSMUSG00000016194.
    ENSMUST00000161737; ENSMUSP00000125620; ENSMUSG00000016194.
    GeneIDi15483.
    KEGGimmu:15483.
    UCSCiuc007eef.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S75207 mRNA. Translation: AAB33601.1 .
    X83202 mRNA. Translation: CAA58209.1 .
    X92186 Genomic DNA. Translation: CAA63096.1 .
    CCDSi CCDS15635.1.
    PIRi I56604.
    RefSeqi NP_001038216.1. NM_001044751.1.
    NP_032314.2. NM_008288.2.
    UniGenei Mm.28328.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1Y5M X-ray 2.30 A/B 24-292 [» ]
    1Y5R X-ray 3.00 A/B 24-292 [» ]
    3GMD X-ray 2.28 A/B/C/D/E/F/G/H 26-289 [» ]
    4K26 X-ray 2.21 A/B 24-292 [» ]
    4NMH X-ray 2.90 A/B/C/D 24-292 [» ]
    ProteinModelPortali P50172.
    SMRi P50172. Positions 25-289.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P50172. 7 interactions.
    MINTi MINT-1863132.

    Chemistry

    BindingDBi P50172.
    ChEMBLi CHEMBL3910.

    PTM databases

    PhosphoSitei P50172.

    Proteomic databases

    MaxQBi P50172.
    PaxDbi P50172.
    PRIDEi P50172.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000016338 ; ENSMUSP00000016338 ; ENSMUSG00000016194 .
    ENSMUST00000161737 ; ENSMUSP00000125620 ; ENSMUSG00000016194 .
    GeneIDi 15483.
    KEGGi mmu:15483.
    UCSCi uc007eef.1. mouse.

    Organism-specific databases

    CTDi 3290.
    MGIi MGI:103562. Hsd11b1.

    Phylogenomic databases

    eggNOGi COG1028.
    HOGENOMi HOG000010276.
    HOVERGENi HBG005481.
    InParanoidi P50172.
    KOi K15680.
    OMAi WGGLDML.
    OrthoDBi EOG7353X9.
    PhylomeDBi P50172.
    TreeFami TF329114.

    Enzyme and pathway databases

    BRENDAi 1.1.1.146. 3474.
    Reactomei REACT_207160. Glucocorticoid biosynthesis.

    Miscellaneous databases

    EvolutionaryTracei P50172.
    NextBioi 288336.
    PROi P50172.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P50172.
    Bgeei P50172.
    CleanExi MM_HSD11B1.
    Genevestigatori P50172.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    [Graphical view ]
    Pfami PF00106. adh_short. 1 hit.
    [Graphical view ]
    PRINTSi PR00081. GDHRDH.
    PROSITEi PS00061. ADH_SHORT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, sequencing and tissue-distribution of mouse 11 beta-hydroxysteroid dehydrogenase-1 cDNA."
      Rajan V., Chapman K.E., Lyons V., Jamieson P., Mullins J.J., Edwards C.R., Seckl J.R.
      J. Steroid Biochem. Mol. Biol. 52:141-147(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6 X CBA.
      Tissue: Liver.
    2. "Cloning and primary structure of murine 11 beta-hydroxysteroid dehydrogenase/microsomal carbonyl reductase."
      Oppermann U.C.T., Netter K.J., Maser E.
      Eur. J. Biochem. 227:202-208(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
      Tissue: Liver.
    3. "The sequence of 5' flanking DNA from the mouse 11 beta-hydroxysteroid dehydrogenase type 1 gene and analysis of putative transcription factor binding sites."
      Voice M.W., Seckl J.R., Chapman K.E.
      Gene 181:233-235(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-10.
    4. "Crystal structure of murine 11 beta-hydroxysteroid dehydrogenase 1: an important therapeutic target for diabetes."
      Zhang J., Osslund T.D., Plant M.H., Clogston C.L., Nybo R.E., Xiong F., Delaney J.M., Jordan S.R.
      Biochemistry 44:6948-6957(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 24-291 IN COMPLEXES WITH NADP AND CORTICOSTERONE, SUBUNIT.

    Entry informationi

    Entry nameiDHI1_MOUSE
    AccessioniPrimary (citable) accession number: P50172
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 126 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3