Estradiol 17-beta-dehydrogenase 8 - Mus musculus (Mouse)

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Sequences

References

Cross-references

Entry information

Relevant documents

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Sequences

References

Cross-references

Entry information

Relevant documents

Molecule processing

Regions

Sites

Amino acid modifications

Natural variations

Experimental info

Keywords

Gene Ontology (GO)

Molecule processing

Regions

Sites

Amino acid modifications

Natural variations

Experimental info

Sequence databases

3D structure databases

PTM databases

2-D gel databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

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Reviewed, UniProtKB/Swiss-Prot P50171 (DHB8_MOUSE)

Last modified November 25, 2008. Version 75. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Protein names

Recommended name:
    Estradiol 17-beta-dehydrogenase 8
    EC=1.1.1.62
Alternative name(s):
    Testosterone 17-beta-dehydrogenase 8
    EC=1.1.1.63
    17-beta-hydroxysteroid dehydrogenase 8
      Short name=17-beta-HSD 8
    Protein Ke6
      Short name=Ke-6

Gene names

Name:	Hsd17b8
Synonyms:	H2-Ke6, Hke6

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Sequence length	260 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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Function	Uses estradiol as its preferred substrate.
Catalytic activity	Estradiol-17-beta + NAD(P)(+) = estrone + NAD(P)H. Testosterone + NAD(+) = androst-4-ene-3,17-dione + NADH.
Pathway	Steroid biosynthesis; estrogen biosynthesis.
Tissue specificity	Kidney, liver, testis, ovary, oviduct, uterus, mammary gland, vagina, prostate, clitoral gland and moderately in spleen, heart, dorsal skin, brain and lung.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Biophysicochemical properties	Kinetic parameters: K_M=0.110 µM for estradiol K_M=0.422 µM for testosterone K_M=0.368 µM for estrone K_M=0.360 µM for dihydrotestosterone V_max=0.405 nmol/min/mg enzyme for estradiol as substrate V_max=0.123 nmol/min/mg enzyme for testosterone as substrate V_max=0.186 nmol/min/mg enzyme for estrone as substrate V_max=0.081 nmol/min/mg enzyme for dihydrotestosterone as substrate

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Keywords
Biological process	Lipid synthesis Steroid biosynthesis
Coding sequence diversity	Alternative splicing
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Acetylation
Gene Ontology (GO)
Biological process	androgen metabolic process Ref.5 Inferred from direct assay. Source: MGI estrogen metabolic process Ref.5 Inferred from direct assay. Source: MGI oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	membrane fraction Inferred from direct assay. Source: MGI mitochondrial envelope Inferred from direct assay. Source: MGI plasma membrane Inferred from direct assay. Source: MGI
Molecular function	binding Inferred from electronic annotation. Source: InterPro estradiol 17-beta-dehydrogenase activity Ref.5 Inferred from direct assay. Source: MGI testosterone 17-beta-dehydrogenase activity Ref.5 Inferred from direct assay. Source: MGI
Complete GO annotation...

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Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Chain

1 – 260

260

Estradiol 17-beta-dehydrogenase 8

PRO_0000054599

Nucleotide binding

13 – 38

NAD By similarity

Active site

168

Proton acceptor By similarity

Binding site

155

Substrate By similarity

Modified residue

N6-acetyllysine

Alternative sequence

257 – 260

GLFM → MRPSWGGGQENRTQVVMRK in isoform Long.

VSP_006030

Sequence conflict

G → GSGVPSQ in AAC69902. Ref.3

Sequence conflict

Missing in AAC53573 and AAC53574. Ref.1

Sequence conflict

230

E → EG in AAC53573 and AAC53574. Ref.1

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Sequence		Length	Mass (Da)
Isoform Short [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 19C712FCDD168B08 Show »	FASTA	260	26,645
10 20 30 40 50 60 MASQLRLRSA LALVTGGAGS GIGRAISVRL AAEGAAVAAC DLDGAAAQDT VRLLGSPGSE 70 80 90 100 110 120 DGAPRGKHAA FQADVSQGPA ARRLLEEVQA CFSRPPSVVV SCAGITRDEF LLHMSEEDWD 130 140 150 160 170 180 RVIAVNLKGT FLVTQAAAQA LVSSGGRGSI INISSIIGKV GNIGQTNYAS SKAGVIGLTQ 190 200 210 220 230 240 TAARELGRHG IRCNSVLPGF IATPMTQKMP EKVKDKVTAM IPLGHMGDPE DVADVVAFLA 250 260 SEDSGYITGA SVEVSGGLFM « Hide
Isoform Long [UniParc]. Checksum: 2351D3A8847F7459 Show »		275	28,396
10 20 30 40 50 60 MASQLRLRSA LALVTGGAGS GIGRAISVRL AAEGAAVAAC DLDGAAAQDT VRLLGSPGSE 70 80 90 100 110 120 DGAPRGKHAA FQADVSQGPA ARRLLEEVQA CFSRPPSVVV SCAGITRDEF LLHMSEEDWD 130 140 150 160 170 180 RVIAVNLKGT FLVTQAAAQA LVSSGGRGSI INISSIIGKV GNIGQTNYAS SKAGVIGLTQ 190 200 210 220 230 240 TAARELGRHG IRCNSVLPGF IATPMTQKMP EKVKDKVTAM IPLGHMGDPE DVADVVAFLA 250 260 270 SEDSGYITGA SVEVSGMRPS WGGGQENRTQ VVMRK « Hide

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	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Downregulation of Ke 6, a novel gene encoded within the major histocompatibility complex, in murine polycystic kidney disease." Aziz N., Maxwell M.M., St Jacques B., Brenner B.M. Mol. Cell. Biol. 13:1847-1853(1993) [PubMed: 8441417] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DBA/2J. Tissue: Kidney.
[2]	Erratum Aziz N., Maxwell M.M., St Jacques B., Brenner B.M. Mol. Cell. Biol. 13:6614-6614(1993)
[3]	"Ke 6 gene. Sequence and organization and aberrant regulation in murine polycystic kidney disease." Maxwell M.M., Nearing J., Aziz N. J. Biol. Chem. 270:25213-25219(1995) [PubMed: 7559658] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C57BL/6.
[4]	"Sequence of the mouse major histocomaptibility locus class II region." Rowen L., Qin S., Madan A., Loretz C., James R., Dors M., Mix L., Hall J., Lasky S., Hood L. Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 129/SvJ.
[5]	"Characterization of Ke 6, a new 17beta-hydroxysteroid dehydrogenase, and its expression in gonadal tissues." Fomitcheva J., Baker M.E., Anderson E., Lee G.Y., Aziz N. J. Biol. Chem. 273:22664-22671(1998) [PubMed: 9712896] [Abstract] Cited for: CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
[6]	"Localization of type 8 17beta-hydroxysteroid dehydrogenase mRNA in mouse tissues as studied by in situ hybridization." Pelletier G., Luu-The V., Li S., Labrie F. J. Histochem. Cytochem. 53:1257-1271(2005) [PubMed: 15923359] [Abstract] Cited for: SUBCELLULAR LOCATION.
[7]	"Substrate and functional diversity of lysine acetylation revealed by a proteomics survey." Kim S.C., Sprung R., Chen Y., Xu Y., Ball H., Pei J., Cheng T., Kho Y., Xiao H., Xiao L., Grishin N.V., White M., Yang X.-J., Zhao Y. Mol. Cell 23:607-618(2006) [PubMed: 16916647] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-67, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

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Sequence databases
	U34072 Genomic DNA. Translation: AAC53573.1. U34072 Genomic DNA. Translation: AAC53574.1. AF100956 Genomic DNA. Translation: AAC69902.1.
PIR	A48154.
UniGene	Mm.275452
3D structure databases
HSSP	HSSP built from PDB template 1GZ6 based on UniProtKB P97852.
ModBase	Search...
PTM databases
PhosphoSite	P50171.
2-D gel databases
REPRODUCTION-2DPAGE	P50171.
Genome annotation databases
Ensembl	ENSMUSG00000073422. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:95911. H2-Ke6.
Phylogenomic databases
HOGENOM	P50171.
HOVERGEN	P50171.
Gene expression databases
CleanEx	MM_H2-KE6.
GermOnline	ENSMUSG00000073422. Mus musculus.
Family and domain databases
InterPro	IPR002198. DHase_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DHase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

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Entry name

DHB8_MOUSE

Accession

Primary (citable) accession number: P50171
Secondary accession number(s): Q60958, Q60959, Q9Z1W2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 25, 2008
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Short (identifier: P50171-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Long (identifier: P50171-2)

The sequence of this isoform differs from the canonical sequence as follows:
257-260: GLFM → MRPSWGGGQENRTQVVMRK