P50170 (RDH2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Retinol dehydrogenase 2
EC=1.1.1.105

Alternative name(s):
29 kDa protein
Retinol dehydrogenase type II
Short name=RODH II

Gene names

Name:	Rdh2
Synonyms:	Rodhii

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts on retinol bound on cellular retinol-binding protein (CRBP). Has higher activity with NADP rather than NAD.
Catalytic activity	All-trans-retinol-[cellular-retinol-binding-protein] + NAD+ = all-trans-retinal-[cellular-retinol-binding-protein] + NADH.
Pathway	Cofactor metabolism; retinol metabolism.
Subcellular location	Microsome. Endoplasmic reticulum Potential.
Tissue specificity	Liver > kidney > brain > lung > testis.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Microsome
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	retinoic acid metabolic process Inferred from direct assay Ref.1. Source: RGD retinol metabolic process Inferred from direct assay Ref.1. Source: RGD
Cellular_component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	retinol dehydrogenase activity Inferred from direct assay Ref.1. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 317	317	Retinol dehydrogenase 2		PRO_0000054759
Regions
	Nucleotide binding	33 – 57	25	NADP By similarity
Sites
	Active site	176	1	Proton acceptor By similarity
	Binding site	164	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P50170 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 13BF030FFC17BCB8	FASTA	317	35,597
	10 20 30 40 50 60 MWLYLLALVG LWNLLRLFRE RKVVSHLQDK YVFITGCDSG FGNLLARQLD RRGMRVLAAC 70 80 90 100 110 120 LTEKGAEQLR SKTSDRLETV ILDVTKTESI VAATQWVKER VGNTGLWGLV NNAGISGHLG 130 140 150 160 170 180 PNEWMNKQNI ASVLDVNLLG MIEVTLSTVP LVRKARGRVV NVASIAGRLS FCGGGYCISK 190 200 210 220 230 240 YGVEAFSDSL RRELSYFGVK VAIVEPGFFR TDVTNGVTLS SNFQMLWDQT SSEVREVYGE 250 260 270 280 290 300 NYLASYLKML NGLDQRCNKD LSLVTDCMEH ALTSCHPRTR YSAGWDAKFF YLPMSYLPTF 310 LVDALFYWTS PKPEKAL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a cDNA for a second retinol dehydrogenase type II. Expression of its mRNA relative to type I." Chai X., Zhai Y., Popescu G., Napoli J.L. J. Biol. Chem. 270:28408-28412(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[3]	"Characterization of two P-450 isozymes placed in the rat CYP2D subfamily." Ohishi N., Imaoka S., Suzuki T., Funae Y. Biochim. Biophys. Acta 1158:227-236(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-10. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U33500 mRNA. Translation: AAC52316.1. BC079153 mRNA. Translation: AAH79153.1.
IPI	IPI00195614.
PIR	I55462.
RefSeq	NP_954678.1. NM_199208.1.
UniGene	Rn.94108.
3D structure databases
ProteinModelPortal	P50170.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-4576403.
STRING	10116.ENSRNOP00000039311.
Proteomic databases
PaxDb	P50170.
PRIDE	P50170.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	299511.
KEGG	rno:299511.
UCSC	RGD:735050. rat.
Organism-specific databases
CTD	8608.
RGD	735050. Rdh2.
Phylogenomic databases
eggNOG	COG1028.
HOVERGEN	HBG005482.
InParanoid	P50170.
Enzyme and pathway databases
BRENDA	1.1.1.105. 5301.
UniPathway	UPA00912.
Gene expression databases
ArrayExpress	P50170.
Genevestigator	P50170.
GermOnline	ENSRNOG00000029651. Rattus norvegicus.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR020904. Sc_DH/Rdtase_CS. [Graphical view]
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	645355.

Entry information

Entry name

RDH2_RAT

Accession

Primary (citable) accession number: P50170

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 29, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program