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P50169 (RDH3_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Retinol dehydrogenase 3
EC=1.1.1.105
Alternative name(s):
Retinol dehydrogenase type I
Short name=RODH I
Gene names
Name:Rdh3
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length317 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts on retinol bound on cellular retinol-binding protein (CRBP). Has higher activity with NADP rather than NAD.

Catalytic activity

All-trans-retinol-[cellular-retinol-binding-protein] + NAD+ = all-trans-retinal-[cellular-retinol-binding-protein] + NADH.

Pathway

Cofactor metabolism; retinol metabolism.

Subcellular location

Microsome. Endoplasmic reticulum Potential.

Tissue specificity

Liver >> lung > testis > brain > kidney.

Sequence similarities

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
   Cellular componentEndoplasmic reticulum
Microsome
   LigandNAD
NADP
   Molecular functionOxidoreductase
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processretinol metabolic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentendoplasmic reticulum

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionnucleotide binding

Inferred from electronic annotation. Source: InterPro

retinol dehydrogenase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 317317Retinol dehydrogenase 3
PRO_0000054760

Regions

Nucleotide binding33 – 5725NADP By similarity

Sites

Active site1761Proton acceptor By similarity
Binding site1641Substrate By similarity

Experimental info

Sequence conflict17 – 182LF → FL in AAB07997. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P50169 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 59B66DDC92E4E24D

FASTA31735,662
        10         20         30         40         50         60 
MWLYLLALVG LWNLLRLFRE RKVVSHLQDK YVFITGCDSG FGNLLARQLD RRGMRVLAAC 

        70         80         90        100        110        120 
LTEKGAEQLR SKTSDRLETV ILDVTKTESI VAATQWVKER VGNRGLWGLV NNAGISVPVG 

       130        140        150        160        170        180 
PNEWMRKKDF ASVLDVNLLG VIEVTLNMLP LVRKARGRVV NIASTMGRMS LVGGGYCISK 

       190        200        210        220        230        240 
YGVEAFSDSL RRELTYFGVK VAIIEPGGFK TNVTNMERLS DNLKKLWDQT TEEVKEIYGE 

       250        260        270        280        290        300 
KFQDSYMKAM ESLVNTCSGD LSLVTDCMEH ALTSCHPRTR YSPGWDAKFF YLPMSYLPTF 

       310 
LSDAVIHWGS VKPARAL

« Hide

References

[1]"Cloning of a cDNA for liver microsomal retinol dehydrogenase. A tissue-specific, short-chain alcohol dehydrogenase."
Chai X., Boerman M.H.E.M., Zhai Y., Napoli J.L.
J. Biol. Chem. 270:3900-3904(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
Strain: Sprague-Dawley.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U18762 mRNA. Translation: AAB07997.1.
IPIIPI00195585.
PIRA55884.
UniGeneRn.31786.

3D structure databases

ProteinModelPortalP50169.
ModBaseSearch...

Protein-protein interaction databases

IntActP50169. 1 interaction.

Proteomic databases

PaxDbP50169.
PRIDEP50169.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

RGD631370. Rdh3.

Phylogenomic databases

eggNOGCOG1028.
HOVERGENHBG005482.
InParanoidP50169.
OrthoDBEOG4STS56.

Enzyme and pathway databases

UniPathwayUPA00912.

Gene expression databases

ArrayExpressP50169.
GenevestigatorP50169.
GermOnlineENSRNOG00000004391. Rattus norvegicus.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSPR00081. GDHRDH.
PR00080. SDRFAMILY.
PROSITEPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRDH3_RAT
AccessionPrimary (citable) accession number: P50169
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 3, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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