Reviewed, UniProtKB/Swiss-Prot P50169 (RDH3_RAT)

Names and origin

Protein names

Recommended name:
    Retinol dehydrogenase 3
    EC=1.1.1.105
Alternative name(s):
    Retinol dehydrogenase type I
      Short name=RODH I

Gene names

Name:

Rdh3

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Acts on retinol bound on cellular retinol-binding protein (CRBP). Has higher activity with NADP rather than NAD.
Catalytic activity	Retinol + NAD+ = retinal + NADH.
Pathway	Cofactor metabolism; retinol metabolism.
Subcellular location	Microsome. Endoplasmic reticulum Potential.
Tissue specificity	Liver >> lung > testis > brain > kidney.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Microsome
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	binding Inferred from electronic annotation. Source: InterPro retinol dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 317	317	Retinol dehydrogenase 3		PRO_0000054760
Regions
	Nucleotide binding	33 – 57	25	NADP By similarity
Sites
	Active site	176	1	Proton acceptor By similarity
	Binding site	164	1	Substrate By similarity
Experimental info
	Sequence conflict	17 – 18	2	LF → FL in AAB07997. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P50169-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 59B66DDC92E4E24D	FASTA	317	35,662
	10 20 30 40 50 60 MWLYLLALVG LWNLLRLFRE RKVVSHLQDK YVFITGCDSG FGNLLARQLD RRGMRVLAAC 70 80 90 100 110 120 LTEKGAEQLR SKTSDRLETV ILDVTKTESI VAATQWVKER VGNRGLWGLV NNAGISVPVG 130 140 150 160 170 180 PNEWMRKKDF ASVLDVNLLG VIEVTLNMLP LVRKARGRVV NIASTMGRMS LVGGGYCISK 190 200 210 220 230 240 YGVEAFSDSL RRELTYFGVK VAIIEPGGFK TNVTNMERLS DNLKKLWDQT TEEVKEIYGE 250 260 270 280 290 300 KFQDSYMKAM ESLVNTCSGD LSLVTDCMEH ALTSCHPRTR YSPGWDAKFF YLPMSYLPTF 310 LSDAVIHWGS VKPARAL

References

[1]	"Cloning of a cDNA for liver microsomal retinol dehydrogenase. A tissue-specific, short-chain alcohol dehydrogenase." Chai X., Boerman M.H.E.M., Zhai Y., Napoli J.L. J. Biol. Chem. 270:3900-3904(1995) [PubMed: 7876135] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: Sprague-Dawley. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18762 mRNA. Translation: AAB07997.1.
IPI	IPI00195585.
PIR	A55884.
UniGene	Rn.31786
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	P50169.
Genome annotation databases
Ensembl	ENSRNOT00000005875; ENSRNOP00000005875; ENSRNOG00000004391; Rattus norvegicus. [Genome view]
Organism-specific databases
RGD	631370. Rdh3.
Phylogenomic databases
eggNOG	roNOG11851.
HOVERGEN	P50169.
InParanoid	P50169.
PhylomeDB	P50169.
Enzyme and pathway databases
BRENDA	1.1.1.105. 248.
Gene expression databases
ArrayExpress	P50169.
Genevestigator	P50169.
GermOnline	ENSRNOG00000004391. Rattus norvegicus.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR020904. Sc_DH/Rdtase_CS. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RDH3_RAT

Accession

Primary (citable) accession number: P50169

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	January 19, 2010
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)