P50168 (DHI2_SHEEP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Corticosteroid 11-beta-dehydrogenase isozyme 2
EC=1.1.1.-

Alternative name(s):
11-beta-hydroxysteroid dehydrogenase type 2
Short name=11-DH2
Short name=11-beta-HSD2
NAD-dependent 11-beta-hydroxysteroid dehydrogenase

Gene names

Name:	HSD11B2
Synonyms:	HSD11K

Organism

Ovis aries (Sheep)

Taxonomic identifier

9940 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Ovis

Protein attributes

Sequence length	427 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the conversion of cortisol to the inactive metabolite cortisone. Modulates intracellular glucocorticoid levels, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids.
Catalytic activity	An 11-beta-hydroxysteroid + NAD+ = an 11-oxosteroid + NADH.
Subunit structure	Interacts with ligand-free cytoplasmic NR3C2 By similarity.
Subcellular location	Microsome. Endoplasmic reticulum Potential.
Tissue specificity	Highly expressed in the kidney and adrenal and at lower levels in the colon.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Microsome
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	nucleotide binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 427	427	Corticosteroid 11-beta-dehydrogenase isozyme 2		PRO_0000054631
Regions
	Nucleotide binding	82 – 111	30	NAD By similarity
Sites
	Active site	232	1	Proton acceptor By similarity
	Binding site	219	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P50168 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 4641749BE408E2B1	FASTA	427	46,669
	10 20 30 40 50 60 MESWPWPSGG AWLLVAARAL LQLLRADLRL GRPLLAALAL LAALDWLCQR LLPPLAALAV 70 80 90 100 110 120 LAATGWIVLS RLARPQRLPV ATRAVLITGC DSGFGNATAK KLDAMGFTVL ATVLDLNSPG 130 140 150 160 170 180 ALELRACCSS RLQLLQMDLT KPADISRVLE FTKVHTASTG LWGLVNNAGQ NIFVADAELC 190 200 210 220 230 240 PVATFRTCME VNFFGALEMT KGLLPLLRRS SGRIVTVSSP AGDMPFPCLA AYGTSKAALA 250 260 270 280 290 300 LLMGNFSCEL LPWGVKVSII LPACFKTESV KDVHQWEERK QQLLATLPQE LLQAYGEDYI 310 320 330 340 350 360 EHLNGQFLHS LSQALPDLSP VVDAITDALL AAQPRRRYYP GHGLGLIYFI HYYLPEGCGR 370 380 390 400 410 420 VSCSPSSSVP MCQEHYRLPA WPYLCPGHSP GPRPQTGPLS HCPVSRAHVE QLQQRRFLVP LLFFQVF

References

[1]	"NAD(+)-dependent isoform of 11 beta-hydroxysteroid dehydrogenase. Cloning and characterization of cDNA from sheep kidney." Agarwal A.K., Mune T., Monder C., White P.C. J. Biol. Chem. 269:25959-25962(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney.
[2]	"Cloning of cDNA encoding an NAD(+)-dependent isoform of 11 beta-hydroxysteroid dehydrogenase in sheep kidney." Agarwal A.K., Mune T., Monder C., White P.C. Endocr. Res. 21:389-397(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U14128 mRNA. Translation: AAA93156.1. Sequence problems.
PIR	A55353.
RefSeq	NP_001009460.1. NM_001009460.1.
UniGene	Oar.416.
3D structure databases
ProteinModelPortal	P50168.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	443530.
Organism-specific databases
CTD	443530.
Phylogenomic databases
HOVERGEN	HBG005482.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR020904. Sc_DH/Rdtase_CS. [Graphical view]
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHI2_SHEEP

Accession

Primary (citable) accession number: P50168

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program