Reviewed, UniProtKB/Swiss-Prot P50167 (ARDH_PICST)

Names and origin

Protein names

Recommended name:
    D-arabinitol 2-dehydrogenase [ribulose-forming]
      Short name=ARDH
    EC=1.1.1.250

Gene names

Name:	ARDH
Synonyms:	ARD2
ORF Names:	PICST_65696

Organism

Pichia stipitis (Yeast) [Complete proteome]

Taxonomic identifier

4924 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Pichia

Protein attributes

Sequence length	278 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	D-arabinitol + NAD+ = D-ribulose + NADH.
Pathway	Carbohydrate metabolism; D-arabinitol metabolism.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	D-arabinitol 2-dehydrogenase activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 278	278	D-arabinitol 2-dehydrogenase [ribulose-forming]		PRO_0000054519
Regions
	Nucleotide binding	22 – 44	23	NAD By similarity
Sites
	Active site	181	1	Proton acceptor By similarity
	Binding site	166	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P50167-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 36869165F23964F6	FASTA	278	30,003
	10 20 30 40 50 60 MDYSYANVVP NFRLDGRLAI ITGGSGGLAA VISRALLAQG ADVALIDMNL ERTKSAAKEV 70 80 90 100 110 120 LGWGEETLKG EHASAIGQVS AWSCNIGDAE AVDATFSSIN EHHGKIADLL INTAGYCENF 130 140 150 160 170 180 PAETYPATNA ESIMKVNGLG SFYVSQSFAR PLIQNNLRGS IILIGSMSGT IVNDPQPQCM 190 200 210 220 230 240 YNMSKAGVIH LVRSLACEWA KYNIRVNTLS PGYILTPLTR NVISGHTEMK EAWESKIPMK 250 260 270 RMAEPKEFVG SILYLASETA SSYTTGHNLV VDGGYECW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A short-chain dehydrogenase gene from Pichia stipitis having D-arabinitol dehydrogenase activity." Hallborn J., Walfridsson M., Penttilae M., Keraenen S., Hahn-Haegerdal B. Yeast 11:839-847(1995) [PubMed: 7483848] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: ATCC 58785 / CBS 6054 / IFO 10063 / NRRL Y-11545.
[2]	"Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis." Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A., Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.-S., Passoth V., Richardson P.M. Nat. Biotechnol. 25:319-326(2007) [PubMed: 17334359] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 58785 / CBS 6054 / IFO 10063 / NRRL Y-11545.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z46866 mRNA. Translation: CAA86939.1. CP000499 Genomic DNA. Translation: ABN67006.1.
PIR	S57351.
RefSeq	XP_001385035.1.
3D structure databases
HSSP	HSSP built from PDB template 1FK8 based on UniProtKB Q9ZFY9.
ModBase	Search...
Genome annotation databases
GeneID	4839199.
KEGG	pic:PICST_65696.
Phylogenomic databases
OMA	P50167. IVNDPQP.
Enzyme and pathway databases
BRENDA	1.1.1.250. 81766.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARDH_PICST

Accession

Primary (citable) accession number: P50167
Secondary accession number(s): A3LUR3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)