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Protein

Tropinone reductase 2

Gene

TR2

Organism
Hyoscyamus niger (Black henbane)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the stereospecific reduction of tropinone to pseudotropine.

Catalytic activityi

Pseudotropine + NADP+ = tropinone + NADPH.

Pathwayi: tropane alkaloid biosynthesis

This protein is involved in the pathway tropane alkaloid biosynthesis, which is part of Alkaloid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway tropane alkaloid biosynthesis and in Alkaloid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei146 – 1461SubstrateBy similarity
Active sitei159 – 1591Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi13 – 3725NADPBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00330.

Names & Taxonomyi

Protein namesi
Recommended name:
Tropinone reductase 2 (EC:1.1.1.236)
Alternative name(s):
Tropinone reductase II
Short name:
TR-II
Gene namesi
Name:TR2
OrganismiHyoscyamus niger (Black henbane)
Taxonomic identifieri4079 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeHyoscyameaeHyoscyamus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 260260Tropinone reductase 2PRO_0000054787Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP50164.
SMRiP50164. Positions 2-260.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50164-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGRWNLEGC TALVTGGSRG IGYGIVEELA NLGASVYTCS RNQKELDECL
60 70 80 90 100
TQWRSKGFNV EASVCDLSSR SEREEFMKTV SNHFHGKLNI LVNNAGIVIY
110 120 130 140 150
KEAKDYTMED YSHIMSINFE AAYHLSVLAH PFLKASERGN VVFISSISGA
160 170 180 190 200
SALPYEAVYG ATKGAMDQLT RCLAFEWAKD NIRVNGVGPG VIATSMVEMT
210 220 230 240 250
IQDPEQKENL DKLIDRCALR RMGEPKELAA VVAFLCFPAA SYVTGQIIYV
260
DGGFMANGGF
Length:260
Mass (Da):28,437
Last modified:October 1, 1996 - v1
Checksum:i6CA7AF85CAA128FC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L20485 mRNA. Translation: AAB09776.1.
AB026545 Genomic DNA. Translation: BAA85845.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L20485 mRNA. Translation: AAB09776.1.
AB026545 Genomic DNA. Translation: BAA85845.1.

3D structure databases

ProteinModelPortaliP50164.
SMRiP50164. Positions 2-260.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00330.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "cDNA encoding tropinone reductase-II from Hyoscyamus niger."
    Nakajima K., Hashimoto T., Yamada Y.
    Plant Physiol. 103:1465-1466(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Cultured root.
  2. "Molecular cloning, expression and characterization of tropinone reductase II, an enzyme of the SDR family in Solanum tuberosum (L.)."
    Keiner R., Kaiser H., Nakajima K., Hashimoto T., Drager B.
    Plant Mol. Biol. 48:299-308(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiTRN2_HYONI
AccessioniPrimary (citable) accession number: P50164
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 11, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.